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UNC-authored articles published by Frontiers
Works (762)
501. Some Good and Some Bad: Sand Fly Salivary Proteins in the Control of Leishmaniasis and in Autoimmunity
- Title Tesim:
- Some Good and Some Bad: Sand Fly Salivary Proteins in the Control of Leishmaniasis and in Autoimmunity
- Creator:
- Cecilio, Pedro, Aoki, Valeria, Diaz, Luis A., Abdeladhim, Maha, Prisayanh, Phillip, Li, Ning, and Valenzuela, Jesus G.
- Date of publication:
- 2022
- Abstract Tesim:
- Sand flies are hematophagous insects responsible for the transmission of vector-borne diseases to humans. Prominent among these diseases is Leishmaniasis that affects the skin and mucous surfaces and organs such as liver and spleen. Importantly, the function of blood-sucking arthropods goes beyond merely transporting pathogens. The saliva of vectors of disease contains pharmacologically active components that facilitate blood feeding and often pathogen establishment. Transcriptomic and proteomic studies have enumerated the repertoire of sand fly salivary proteins and their potential use for the control of Leishmaniasis, either as biomarkers of vector exposure or as anti-Leishmania vaccines. However, a group of specific sand fly salivary proteins triggers formation of cross-reactive antibodies that bind the ectodomain of human desmoglein 1, a member of the epidermal desmosomal cadherins. These cross-reactive antibodies are associated with skin autoimmune blistering diseases, such as pemphigus, in certain immunogenetically predisposed individuals. In this review, we focus on two different aspects of sand fly salivary proteins in the context of human disease: The good, which refers to salivary proteins functioning as biomarkers of exposure or as anti-Leishmania vaccines, and the bad, which refers to salivary proteins as environmental triggers of autoimmune skin diseases.
- Resource type:
- Article
- Affiliation Label Tesim:
- Department of Dermatology
- DOI:
- https://doi.org/10.17615/qyqk-7s23
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fcimb.2022.839932
- ISSN:
- 2235-2988
- Journal Title:
- Frontiers in Cellular and Infection Microbiology
- Journal Volume:
- 12
- Keyword:
- antibodies, cellular immunity, salivary proteins, immunogenicity, sand fly, and autoimmunity
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Other Affiliation:
- National Institutes of Health and Universidade de Sao Paulo
- Person:
- Cecilio, Pedro, Aoki, Valeria, Diaz, Luis A., Abdeladhim, Maha, Prisayanh, Phillip, Li, Ning, and Valenzuela, Jesus G.
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_12
502. Microbial Hydrocarbon Degradation in Guaymas Basin—Exploring the Roles and Potential Interactions of Fungi and Sulfate-Reducing Bacteria
- Title Tesim:
- Microbial Hydrocarbon Degradation in Guaymas Basin—Exploring the Roles and Potential Interactions of Fungi and Sulfate-Reducing Bacteria
- Creator:
- Mara, Paraskevi, Edgcomb, Virginia P., and Teske, Andreas P.
- Date of publication:
- 2022
- Abstract Tesim:
- Hydrocarbons are degraded by specialized types of bacteria, archaea, and fungi. Their occurrence in marine hydrocarbon seeps and sediments prompted a study of their role and their potential interactions, using the hydrocarbon-rich hydrothermal sediments of Guaymas Basin in the Gulf of California as a model system. This sedimented vent site is characterized by localized hydrothermal circulation that introduces seawater sulfate into methane- and hydrocarbon-rich sediments, and thus selects for diverse hydrocarbon-degrading communities of which methane, alkane- and aromatics-oxidizing sulfate-reducing bacteria and archaea have been especially well-studied. Current molecular and cultivation surveys are detecting diverse fungi in Guaymas Basin hydrothermal sediments, and draw attention to possible fungal-bacterial interactions. In this Hypothesis and Theory article, we report on background, recent results and outcomes, and underlying hypotheses that guide current experiments on this topic in the Edgcomb and Teske labs in 2021, and that we will revisit during our ongoing investigations of bacterial, archaeal, and fungal communities in the deep sedimentary subsurface of Guaymas Basin.
- Resource type:
- Article
- Affiliation Label Tesim:
- University of North Carolina at Chapel Hill
- DOI:
- https://doi.org/10.17615/pe48-yv11
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fmicb.2022.831828
- ISSN:
- 1664-302X
- Journal Title:
- Frontiers in Microbiology
- Journal Volume:
- 13
- Keyword:
- sulfate-reducing bacteria, microbial interaction, fungi, Guaymas Basin, and hydrocarbon
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Other Affiliation:
- Woods Hole Oceanographic Institution
- Person:
- Mara, Paraskevi, Edgcomb, Virginia P., and Teske, Andreas P.
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_11
503. Regional Differences in Mucociliary Clearance in the Upper and Lower Airways
- Title Tesim:
- Regional Differences in Mucociliary Clearance in the Upper and Lower Airways
- Creator:
- Grubb, Barbara R., Rogers, Troy D., Button, Brian, Esther, Charles R., Kelada, Samir N. P., Ostrowski, Lawrence E., Gutay, Mark I., and Livraghi-Butrico, Alessandra
- Date of publication:
- 2022
- Abstract Tesim:
- As the nasal cavity is the portal of entry for inspired air in mammals, this region is exposed to the highest concentration of inhaled particulate matter and pathogens, which must be removed to keep the lower airways sterile. Thus, one might expect vigorous removal of these substances via mucociliary clearance (MCC) in this region. We have investigated the rate of MCC in the murine nasal cavity compared to the more distal airways (trachea). The rate of MCC in the nasal cavity (posterior nasopharynx, PNP) was ∼3–4× greater than on the tracheal wall. This appeared to be due to a more abundant population of ciliated cells in the nasal cavity (∼80%) compared to the more sparsely ciliated trachea (∼40%). Interestingly, the tracheal ventral wall exhibited a significantly lower rate of MCC than the tracheal posterior membrane. The trachealis muscle underlying the ciliated epithelium on the posterior membrane appeared to control the surface architecture and likely in part the rate of MCC in this tracheal region. In one of our mouse models (Bpifb1 KO) exhibiting a 3-fold increase in MUC5B protein in lavage fluid, MCC particle transport on the tracheal walls was severely compromised, yet normal MCC occurred on the tracheal posterior membrane. While a blanket of mucus covered the surface of both the PNP and trachea, this mucus appeared to be transported as a blanket by MCC only in the PNP. In contrast, particles appeared to be transported as discrete patches or streams of mucus in the trachea. In addition, particle transport in the PNP was fairly linear, in contrast transport of particles in the trachea often followed a more non-linear route. The thick, viscoelastic mucus blanket that covered the PNP, which exhibited ∼10-fold greater mass of mucus than did the blanket covering the surface of the trachea, could be transported over large areas completely devoid of cells (made by a breach in the epithelial layer). In contrast, particles could not be transported over even a small epithelial breach in the trachea. The thick mucus blanket in the PNP likely aids in particle transport over the non-ciliated olfactory cells in the nasal cavity and likely contributes to humidification and more efficient particle trapping in this upper airway region.
- Resource type:
- Article
- Affiliation Label Tesim:
- Marsico Lung Institute/UNC Cystic Fibrosis Center
- DOI:
- https://doi.org/10.17615/8cvq-yz24
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fphys.2022.842592
- ISSN:
- 1664-042X
- Journal Title:
- Frontiers in Physiology
- Journal Volume:
- 13
- Keyword:
- mucus blanket, tracheal walls, tracheal posterior membrane, mucociliary clearance, and posterior nasopharynx
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Person:
- Grubb, Barbara R., Rogers, Troy D., Button, Brian, Esther, Charles R., Kelada, Samir N. P., Ostrowski, Lawrence E., Gutay, Mark I., and Livraghi-Butrico, Alessandra
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_10
504. Microglia-Specific Promoter Activities of HEXB Gene
- Title Tesim:
- Microglia-Specific Promoter Activities of HEXB Gene
- Creator:
- Tang, Yuyang, Garcia, J. Victor, Bodnar, Brittany, Saribas, Sami, Wong, Lilly M., Shah, Sahil, Wang, Hong, Hu, Wenhui, Wei, Zhengyu, Jiang, Guochun, Wang, Xianwei, Margolis, David M., Ellis, Kendra, Ling, Binhua, and Ting, Julia
- Date of publication:
- 2022
- Abstract Tesim:
- Adeno-associated virus (AAV)-mediated genetic targeting of microglia remains a challenge. Overcoming this hurdle is essential for gene editing in the central nervous system (CNS). Here, we characterized the minimal/native promoter of the HEXB gene, which is known to be specifically and stably expressed in the microglia during homeostatic and pathological conditions. Dual reporter and serial deletion assays identified the critical role of the natural 5’ untranslated region (−97 bp related to the first ATG) in driving transcriptional activity of the mouse Hexb gene. The native promoter region of mouse, human, and monkey HEXB are located at −135, −134, and −170 bp to the first ATG, respectively. These promoters were highly active and specific in microglia with strong cross-species transcriptional activities, but did not exhibit activity in primary astrocytes. In addition, we identified a 135 bp promoter of CD68 gene that was highly active in microglia but not in astrocytes. Considering that HEXB is specifically expressed in microglia, these data suggest that the newly characterized microglia-specific HEXB minimal/native promoter can be an ideal candidate for microglia-targeting AAV gene therapy in the CNS.
- Resource type:
- Article
- Affiliation Label Tesim:
- HIV Cure Center, Center for AIDS Research, and Department of Medicine
- DOI:
- https://doi.org/10.17615/7av7-5v14
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fncel.2022.808598
- ISSN:
- 1662-5102
- Journal Title:
- Frontiers in Cellular Neuroscience
- Journal Volume:
- 16
- Keyword:
- CD68, gene therapy, microglia, astrocytes, HexB, and gene editing
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Other Affiliation:
- Temple University Lewis Katz School of Medicine and Texas Biomedical Research Institute
- Person:
- Tang, Yuyang, Garcia, J. Victor, Bodnar, Brittany, Saribas, Sami, Wong, Lilly M., Shah, Sahil, Wang, Hong, Hu, Wenhui, Wei, Zhengyu, Jiang, Guochun, Wang, Xianwei, Margolis, David M., Ellis, Kendra, Ling, Binhua, and Ting, Julia
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_9
505. Clinical Team Response to the Impact of COVID-19 on Diabetes Self-Management: Findings From a Qualitative Study
- Title Tesim:
- Clinical Team Response to the Impact of COVID-19 on Diabetes Self-Management: Findings From a Qualitative Study
- Creator:
- Donahue, Katrina E., Young, Laura, Rees, Jennifer, Cameron, Thomas C., Richman, Erica, Johnson, Asia, Hale, Lily, Vu, Maihan B., and Leeman, Jennifer
- Date of publication:
- 2022
- Abstract Tesim:
- The aims of this study were to explore providers’ perceptions of how COVID-19 affected patients’ psychological wellbeing and diabetes self-care and discover how providers responded to sustain and improve patients’ psychological health and diabetes management during the pandemic. Twenty-four semi-structured interviews were completed with primary care providers (n=14) and endocrine specialty clinicians (n=10) across sixteen clinics in North Carolina. Interview topics included: (1) current glucose monitoring approaches and diabetes management strategies for people with diabetes (2) barriers and unintended consequences encountered with respect to diabetes self-management, and (3) innovative strategies developed to overcome barriers. Interview transcripts were coded using qualitative analysis software and analyzed to identify cross-cutting themes and differences between participants. Primary care providers and endocrine specialty clinicians reported that people with diabetes experienced increased mental health symptoms, increased financial challenges and positive and negative changes in self-care routines due to COVID-19. To offer support, primary care providers and endocrine specialty providers focused discussions on lifestyle management and utilized telemedicine to connect with patients. Additionally, endocrine specialty clinicians helped patients access financial assistance programs. Findings indicate that people with diabetes experienced unique challenges to self-management during the pandemic and providers responded with targeted support strategies. Future research should explore the effectiveness of these provider interventions as the pandemic continues to evolve.
- Resource type:
- Article
- Affiliation Label Tesim:
- Department of Family Medicine, Department of Medicine, North Carolina Translational and Clinical Sciences Institute, Cecil G. Sheps Center for Health Services Research, School of Medicine, Department of Health Behavior, and School of Nursing
- DOI:
- https://doi.org/10.17615/hwzj-nw40
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fcdhc.2022.835845
- ISSN:
- 2673-6616
- Journal Title:
- Frontiers in Clinical Diabetes and Healthcare
- Journal Volume:
- 3
- Keyword:
- qualitative research, mental health, COVID-19, self-management, self-care, and diabetes
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Person:
- Donahue, Katrina E., Young, Laura, Rees, Jennifer, Cameron, Thomas C., Richman, Erica, Johnson, Asia, Hale, Lily, Vu, Maihan B., and Leeman, Jennifer
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_8
506. Human Immunodeficiency Virus-1 Latency Reversal via the Induction of Early Growth Response Protein 1 to Bypass Protein Kinase C Agonist-Associated Immune Activation
- Title Tesim:
- Human Immunodeficiency Virus-1 Latency Reversal via the Induction of Early Growth Response Protein 1 to Bypass Protein Kinase C Agonist-Associated Immune Activation
- Creator:
- Wong, Lilly M., Tang, Yuyang, Li, Dajiang, Dandekar, Satya, Méndez-Lagares, Gema, Thompson, George R., Jiang, Guochun, and Hartigan-O'Connor, Dennis J.
- Date of publication:
- 2022
- Abstract Tesim:
- Human Immunodeficiency Virus-1 (HIV) remains a global health challenge due to the latent HIV reservoirs in people living with HIV (PLWH). Dormant yet replication competent HIV harbored in the resting CD4+ T cells cannot be purged by antiretroviral therapy (ART) alone. One approach of HIV cure is the “Kick and Kill” strategy where latency reversal agents (LRAs) have been implemented to disrupt latent HIV, expecting to eradicate HIV reservoirs by viral cytopathic effect or immune-mediated clearance. Protein Kinase C agonists (PKCa), a family of LRAs, have demonstrated the ability to disrupt latent HIV to an extent. However, the toxicity of PKCa remains a concern in vivo. Early growth response protein 1 (EGR1) is a downstream target of PKCa during latency reversal. Here, we show that PKCa induces EGR1 which directly drives Tat-dependent HIV transcription. Resveratrol, a natural phytoalexin found in grapes and various plants, induces Egr1 expression and disrupts latent HIV in several HIV latency models in vitro and in CD4+ T cells isolated from ART-suppressed PLWH ex vivo. In the primary CD4+ T cells, resveratrol does not induce immune activation at the dosage that it reverses latency, indicating that targeting EGR1 may be able to reverse latency and bypass PKCa-induced immune activation.
- Resource type:
- Article
- Affiliation Label Tesim:
- HIV Cure Center
- DOI:
- https://doi.org/10.17615/p3g5-8b11
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fmicb.2022.836831
- ISSN:
- 1664-302X
- Journal Title:
- Frontiers in Microbiology
- Journal Volume:
- 13
- Keyword:
- immune response, kick and kill, HIV reservoir, HIV latency, EGR1, protein kinase C, and HIV cure
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Other Affiliation:
- University of California, Davis
- Person:
- Wong, Lilly M., Tang, Yuyang, Li, Dajiang, Dandekar, Satya, Méndez-Lagares, Gema, Thompson, George R., Jiang, Guochun, and Hartigan-O'Connor, Dennis J.
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_7
507. Reduction of Stabilin-2 Contributes to a Protection Against Atherosclerosis
- Title Tesim:
- Reduction of Stabilin-2 Contributes to a Protection Against Atherosclerosis
- Creator:
- Li, Feng, Hagaman, John, Lewis, Amanda M., Kayashima, Yukako, Sun, Xinghui, Huynh, Phillip, Wilder, Jennifer, Hiller, Sylvia, Maeda-Smithies, Nobuyo, Clanton, Connor A., and Harris, Edward N.
- Date of publication:
- 2022
- Abstract Tesim:
- We have previously identified a novel atherosclerosis quantitative trait locus (QTL), Arch atherosclerosis 5 (Aath5), on mouse chromosome 10 by three-way QTL analyses between Apoe−/− mice on a DBA/2J, 129S6 and C57BL/6J background. The DBA/2J haplotype at the Aath5 locus was associated with smaller plaque size. One of the candidate genes underlying Aath5 was Stabilin-2 (Stab2), which encodes a clearance receptor for hyaluronan (HA) predominantly expressed in liver sinusoidal endothelial cells (LSECs). However, the role of Stab2 in atherosclerosis is unknown. A congenic line of Apoe−/− mice carrying Aath5 covering the Stab2DBA allele on a background of 129S6 confirmed the small reductions of atherosclerotic plaque development. To further determine whether Stab2 is an underlying gene for Aath5, we generated Stab2−/−Apoe−/− mice on a C57BL/6J background. When fed with a Western diet for 8 weeks, Stab2−/−Apoe−/− males developed approximately 30% smaller plaques than Stab2+/+Apoe−/− mice. HA was accumulated in circulation but not in major organs in the Stab2 deficient mice. STAB2-binding molecules that are involved in atherosclerosis, including acLDL, apoptotic cells, heparin and vWF were not likely the direct cause of the protection in the Stab2−/−Apoe−/− males. These data indicate that reduction of Stab2 is protective against atherosclerotic plaque development, and that Stab2 is a contributing gene underlying Aath5, although its effect is small. To test whether non-synonymous amino acid changes unique to DBA/2J affect the function of STAB2 protein, we made HEK293 cell lines expressing STAB2129 or STAB2DBA proteins, as well as STAB2129 proteins carrying each of five DBA-unique replacements that have been predicted to be deleterious. These mutant cells were capable of internalizing 125I -HA and DiI-acLDL similarly to the control cells. These results indicate that the amino acid changes unique to DBA/2J are not affecting the function of STAB2 protein, and support our previous observation that the reduced transcription of Stab2 in the liver sinusoid as a consequence of the insertion of a viral-derived sequence, intracisternal A particle, is the primary contributor to the athero-protection conferred by the DBA/2J allele.
- Resource type:
- Article
- Affiliation Label Tesim:
- Department of Pathology and Laboratory Medicine
- DOI:
- https://doi.org/10.17615/37zt-x391
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fcvm.2022.818662
- ISSN:
- 2297-055X
- Journal Title:
- Frontiers in Cardiovascular Medicine
- Journal Volume:
- 9
- Keyword:
- hyaluronic acid, aorta, stabilin 2, mouse, and atherosclerosis
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Other Affiliation:
- University of Nebraska
- Person:
- Li, Feng, Hagaman, John, Lewis, Amanda M., Kayashima, Yukako, Sun, Xinghui, Huynh, Phillip, Wilder, Jennifer, Hiller, Sylvia, Maeda-Smithies, Nobuyo, Clanton, Connor A., and Harris, Edward N.
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_6
508. HIV Co-infection Augments EBV-Induced Tumorigenesis in vivo
- Title Tesim:
- HIV Co-infection Augments EBV-Induced Tumorigenesis in vivo
- Creator:
- Spagnuolo, Rae Ann, Whitehurst, Christopher B., Wahl, Angela, Teklezghi, Adonay, Rizk, Monica, and Pagano, Joseph S.
- Date of publication:
- 2022
- Abstract Tesim:
- In most individuals, EBV maintains a life-long asymptomatic latent infection. However, EBV can induce the formation of B cell lymphomas in immune suppressed individuals including people living with HIV (PLWH). Most individuals who acquire HIV are already infected with EBV as EBV infection is primarily acquired during childhood and adolescence. Although antiretroviral therapy (ART) has substantially reduced the incidence of AIDS-associated malignancies, EBV positive PLWH are at an increased risk of developing lymphomas compared to the general population. The direct effect of HIV co-infection on EBV replication and EBV-induced tumorigenesis has not been experimentally examined. Using a humanized mouse model of EBV infection, we demonstrate that HIV co-infection enhances systemic EBV replication and immune activation. Importantly, EBV-induced tumorigenesis was augmented in EBV/HIV co-infected mice. Collectively, these results demonstrate a direct effect of HIV co-infection on EBV pathogenesis and disease progression and will facilitate future studies to address why the incidence of certain types of EBV-associated malignancies are stable or increasing in ART treated PLWH.
- Resource type:
- Article
- Affiliation Label Tesim:
- Department of Medicine and Department of Microbiology and Immunology
- DOI:
- https://doi.org/10.17615/0wav-zw72
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fviro.2022.861628
- ISSN:
- 2673-818X
- Journal Title:
- Frontiers in Virology
- Journal Volume:
- 2
- Keyword:
- human immunodeficiency virus (HIV), co-infection, replication, Epstein-Barr Virus (EBV), B cell lymphoma, tumorigenesis, and humanized mice
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Person:
- Spagnuolo, Rae Ann, Whitehurst, Christopher B., Wahl, Angela, Teklezghi, Adonay, Rizk, Monica, and Pagano, Joseph S.
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_5
509. Anti-Tumor and Anti-Invasive Effects of ONC201 on Ovarian Cancer Cells and a Transgenic Mouse Model of Serous Ovarian Cancer
- Title Tesim:
- Anti-Tumor and Anti-Invasive Effects of ONC201 on Ovarian Cancer Cells and a Transgenic Mouse Model of Serous Ovarian Cancer
- Creator:
- Pierce, Stuart R., Prabhu, Varun, Sun, Wenchuan, Allen, Joshua E., Staley, Allison, Fang, Ziwei, Zhou, Chunxiao, Yin, Yajie, Fan, Yali, Bae-Jump, Victoria L., Tucker, Katherine, Kong, Weimin, Wang, Jiandong, and West, Lindsay
- Date of publication:
- 2022
- Abstract Tesim:
- ONC201 is a promising first-in-class small molecule that has been reported to have anti-neoplastic activity in various types of cancer through activation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) as well as activation of mitochondrial caseinolytic protease P (ClpP). The present study was to explore the anti-tumor potential effect of ONC201 in ovarian cancer cell lines and in a transgenic mouse model of high grade serous ovarian cancer under obese (high fat diet) and lean (low fat diet) conditions. ONC201 significantly suppressed cell proliferation, induced arrest in G1 phase, and increased cellular stress and apoptosis, accompanied by dual inhibition of the AKT/mTOR/S6 and MAPK pathways in OC cells. ONC201 also resulted in inhibition of adhesion and invasion via epithelial–mesenchymal transition and reduction of VEGF expression. Pre-treatment with the anti-oxidant, N-acetylcysteine (NAC), reversed the ONC201-induced oxidative stress response, and prevented ONC201-reduced VEGF and cell invasion by regulating epithelial–mesenchymal transition protein expression. Knockdown of ClpP in ovarian cancer cells reduced ONC201 mediated the anti-tumor activity and cellular stress. Diet-induced obesity accelerated ovarian tumor growth in the KpB mouse model. ONC201 significantly suppressed tumor growth, and decreased serum VEGF production in obese and lean mice, leading to a decrease in tumoral expression of Ki-67, VEGF and phosphorylation of p42/44 and S6 and an increase in ClpP and DRD5, as assessed by immunohistochemistry. These results suggest that ONC201 may be a promising therapeutic agent to be explored in future clinical trials in high-grade serous ovarian cancer.
- Resource type:
- Article
- Affiliation Label Tesim:
- University of North Carolina at Chapel Hill
- DOI:
- https://doi.org/10.17615/8rts-tb68
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fonc.2022.789450
- ISSN:
- 2234-943X
- Journal Title:
- Frontiers in Oncology
- Journal Volume:
- 12
- Keyword:
- invasion, proliferation, DRD2, ONC201, and ovarian cancer
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Other Affiliation:
- Oncoceutics and Capital Medical University
- Person:
- Pierce, Stuart R., Prabhu, Varun, Sun, Wenchuan, Allen, Joshua E., Staley, Allison, Fang, Ziwei, Zhou, Chunxiao, Yin, Yajie, Fan, Yali, Bae-Jump, Victoria L., Tucker, Katherine, Kong, Weimin, Wang, Jiandong, and West, Lindsay
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_4
510. Exploring Mental Health and Academic Outcomes of Children Receiving Non-manualized, Transdiagnostic, Task-Shifted Mental Health Care From Their Teachers in a Low-and-Middle Income Country
- Title Tesim:
- Exploring Mental Health and Academic Outcomes of Children Receiving Non-manualized, Transdiagnostic, Task-Shifted Mental Health Care From Their Teachers in a Low-and-Middle Income Country
- Creator:
- Thapa, Arpana, Vanderburg, Juliana L., Hampanda, Karen, Rauniyar, Abhishek K., Gaynes, Bradley N., Giri, Priscilla, Bhattarai, Surekha, Matergia, Michael, Lamb, Molly M., Cruz, Christina M., and Dukpa, Choden
- Date of publication:
- 2022
- Abstract Tesim:
- A majority of children worldwide who face mental health difficulties, especially in low-and-middle income countries, remain undiagnosed and untreated. This deficit roots in part from a lack of trained professionals qualified to provide care. Task-shifting the provision of treatment to teachers, individuals with consistent access to children, can reduce the care gap. The current study investigated whether the implementation of a pilot trial of Tealeaf-Mansik Swastha (Teachers Leading the Frontlines—Mental Health; “Tealeaf”) was associated with improvements in child mental health and academic outcomes. Tealeaf is a transdiagnostic, non-manualized, task-shifting intervention in which teachers identify students in need of mental health care and then provide task-shifted care for them using an emerging, novel therapy modality, “education as mental health therapy” (Ed-MH). Pre-post standardized quantitative measures focused on child mental health status and academics. The measures were completed by multiple raters and compared to determine whether changes occurred. Results indicated that primary teacher raters observed significant improvements in child mental health symptoms overall, while secondary teacher raters and caregivers noted improvement for certain diagnostic categories. Caregivers observed on average a decreased impact of their children's mental health symptoms on their children's lives. Academically, math scores significantly improved while reading trended toward significance. Preliminary evidence overall supports the viability of Tealeaf and Ed-MH for positively impacting child mental health and academics. Future directions include the implementation of a formalized, randomized-controlled trial to strengthen preliminary outcomes.
- Resource type:
- Article
- Affiliation Label Tesim:
- School Psychology Graduate Program and Department of Psychiatry
- DOI:
- https://doi.org/10.17615/3ma6-p510
- Edition:
- Publisher
- Identifier:
- https://dx.doi.org/10.3389/fped.2022.807178
- ISSN:
- 2296-2360
- Journal Title:
- Frontiers in Pediatrics
- Journal Volume:
- 10
- Keyword:
- child mental health, low-and-middle income countries (LMIC), school-based intervention, task-shifting, and teachers
- Language Label:
- English
- License Label:
- Attribution 4.0 International
- Other Affiliation:
- Darjeeling Ladenla Road Prerna and Colorado School of Public Health
- Person:
- Thapa, Arpana, Vanderburg, Juliana L., Hampanda, Karen, Rauniyar, Abhishek K., Gaynes, Bradley N., Giri, Priscilla, Bhattarai, Surekha, Matergia, Michael, Lamb, Molly M., Cruz, Christina M., and Dukpa, Choden
- Rights Statement Label:
- In Copyright
- Source:
- frontiers_backfiles_3
Collection Details
- Total items
-
762
- Size
-
unknown
- Date created
-
May 26, 2022