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Works (1066)

31. Eicosapentaenoic Acid Suppresses Tumor Growth and Enhances Chemosensitivity via AKT/mTOR Signaling in Uterine Serous Carcinoma.

Title Tesim:: Eicosapentaenoic Acid Suppresses Tumor Growth and Enhances Chemosensitivity via AKT/mTOR Signaling in Uterine Serous Carcinoma.
Creator:: Zhou, Chunxiao, Zhang, Haomeng, Bae-Jump, Victoria L, Singleton, Miller, Boyles, Glenn, Kong, Weimin, Shen, Xiaochang, Diggs, Alexandra, Vranes, Chelsey, and Chen, Shuning
Date of publication:: 2026
Abstract Tesim:: Uterine serous carcinoma (USC) is an aggressive subtype of endometrial cancer characterized by high recurrence rates and poor response to conventional therapies, resulting in unfavorable clinical outcomes. Eicosapentaenoic acid (EPA), an omega-3 polyunsaturated fatty acid, has demonstrated anti-cancer activity in multiple malignancies.This study used two USC cell lines, ARK1 and SPEC2, to evaluate the effects of EPA on cell proliferation, invasion, cell cycle profile, stress response, and apoptosis. The potential synergistic effects of EPA combined with carboplatin were also examined. Western blotting was used to examine EPA's effects on downstream pathways related to cellular stress, inflammation, and epithelial-mesenchymal transition (EMT).EPA treatment markedly reduced cell proliferation and colony formation, with IC50 values of 28.96 µM for ARK-1 cells and 14.96 µM for SPEC-2 cells compared with control groups. It also induced G1 phase cell cycle arrest, increased cellular stress, triggered caspase-dependent apoptotic cell death, and suppressed invasive capacity. Moreover, EPA effectively counteracted TNF-α-stimulated upregulation of COX-2 and phosphorylated NF-κB. The combined treatment with EPA and carboplatin resulted in synergistic inhibition of cell viability and migration. Western blotting analysis showed that EPA attenuates the NF-κB and AKT/mTOR signaling pathways, promotes the expression of cellular stress-related proteins, and inhibits the expression of EMT-related proteins in both cell lines.EPA exhibits potent anti-tumor activity against USC cells and enhances the efficacy of carboplatin. These data indicate that EPA has potential as a low-toxicity, multi-target adjuvant treatment for USC, necessitating additional pre-clinical and clinical investigation.
Resource type:: Article
Affiliation Label Tesim:: School of Medicine and Department of Obstetrics and Gynecology
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/stjg-ep48
Edition:: Publisher
Identifier:: DOI: https://dx.doi.org/10.3390/cancers18071120, PMCID: PMC13072313, and PMID: 41976343
ISSN:: 2072-6694
Journal Issue:: 7
Journal Title:: Cancers
Journal Volume:: 18
Keyword:: eicosapentaenoic acid (EPA), apoptosis, synergy, uterine serous carcinoma, and invasion
License Label:: Attribution 4.0 International
ORCID:: https://orcid.org/0000-0002-1257-9537, , https://orcid.org/0000-0001-6778-0951, and https://orcid.org/0000-0001-9648-9864
Other Affiliation:: and Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China.
Person:: Zhou, Chunxiao, Zhang, Haomeng, Bae-Jump, Victoria L, Singleton, Miller, Boyles, Glenn, Kong, Weimin, Shen, Xiaochang, Diggs, Alexandra, Vranes, Chelsey, and Chen, Shuning
Rights Statement Label:: In Copyright

32. Deciphering RTK-RAS and MAPK Pathway Dependencies in Gemcitabine-Treated Pancreatic Ductal Adenocarcinoma Through Conversational Artificial Intelligence

Title Tesim:: Deciphering RTK-RAS and MAPK Pathway Dependencies in Gemcitabine-Treated Pancreatic Ductal Adenocarcinoma Through Conversational Artificial Intelligence
Creator:: Carranza, Francisco G., Waldrup, Brigette, Velazquez-Villarreal, Enrique, Manjarrez, Sophia, and Diaz, Fernando C.
Date of publication:: 2026
Abstract Tesim:: Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy marked by substantial molecular heterogeneity and variable response to gemcitabine-based therapy. While KRAS mutations are nearly universal, the broader RTK-RAS and MAPK signaling architecture and its relationship to treatment response remain incompletely defined. We conducted an integrative clinical-genomic analysis of 184 PDAC tumors stratified by age at diagnosis and gemcitabine exposure, interrogating somatic alterations across curated RTK-RAS/MAPK gene sets. Conversational artificial intelligence agents (AI-HOPE-RTK-RAS and AI-HOPE-MAPK) enabled dynamic cohort construction and pathway-level analyses, with findings validated using standard statistical methods. In late-onset PDAC, ERBB2 and RET mutations were significantly enriched in gemcitabine-treated tumors. Early-onset cases demonstrated differential enrichment of CACNA2D family alterations in non-treated tumors and higher frequencies of FLNB and TP53 mutations in treated disease. Importantly, late-onset patients not treated with gemcitabine who lacked RTK-RAS or MAPK alterations exhibited significantly improved overall survival. These findings reveal age- and treatment-dependent pathway dependencies beyond canonical KRAS status and support a precision oncology framework in PDAC. Conversational AI facilitated rapid, multidimensional clinical–genomic integration to uncover clinically relevant signaling substructures.
Resource type:: Article
Affiliation Label Tesim:: N.C. Cancer Hospital and UNC Lineberger Comprehensive Cancer Center
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/052k-yg27
Edition:: Publisher
Identifier:: PMCID: PMC13072942, PMID: 41977200, and DOI: https://dx.doi.org/10.3390/ijms27073011
ISSN:: 1422-0067
Journal Issue:: 7
Journal Title:: International Journal of Molecular Sciences
Journal Volume:: 27
Keyword:: UK Biobank, auxin transport, mineral distribution, polymer, computed tomography angiography, root morphology, access to dental care, salinity, uveal melanoma, sericin-derived oligopeptides, OECD guidelines, edible coatings, microcephaly, pancreatic ductal adenocarcinoma, operative vaginal delivery, cellular senescence, simulation, angle closure, thermal–mass coupling, topical therapy, NIR, updated Schwartz bedside formula, food preservation, paper barrier, 4-phenylbutyrate, precision cardiology, hardness, contaminants, hydrogen production, aneurysm, release of pollutants, molecular targets, recombinant inbred lines, wear analysis, genomic medicine, agro-industrial waste, effector protein, CI, conducting polymers, medication de-escalation, PRS, thyroid laser therapy, SEM-RDS, Paspalum vaginatum, telomere length, compression parallel to grain, FNAC, immune-suppressive factor, nanocomposites, in utero exposome, metabolic regulation, multiple organ injuries, wollastonite, electrochemistry, litter decomposition dynamics, tyrosinase, BCR::ABL1, inflammatory bowel disease, quality control, stress indices, dysplasia surveillance, synergistic effect, CML, deodorization mechanism, subacute stroke, co-expression network, maternal stress, hydraulic fracturing, intraoperative neurophysiologic monitoring, polyurethane foams, thyroid cancer, post-synthesis modification, omega-3 fatty acids, development, bone metabolism, tumor suppressor genes, plastics recycling, polyvinylidene fluoride (PVDF), C2C12 myoblasts, interfacial bonding, MnO2/acetylene black cathode, carvacrol, ischemia/reperfusion, human transglutaminases, physiological response, osteocalcin, tauroursodeoxycholic acid, Malus domestica, glucocerebrosidase, endoscopy, shaker K+ channels, PCA, nanocomposite, calcium binding, morpho-physiological traits, SNP, SbMIKC17, chromatography, CDKN1A (p21), gene-set scoring, redox enzymes, HDAC6, epigallocatechin gallate, sugar beet, nanotechnology, single-needle electrospinning, Ideonella sakaiensis, imatinib, orthodontically induced external root resorption, forest management, TMD, chronic disease prognosis, pneumococcus, LC-MS/MS, human papillomavirus, gene density, minced meat, alcohol, immunosuppression, brachial plexus, macrophages, risk assessment, decolorization, elastin, sentinel lymph node biopsy, bite force, QTL mapping, maternal toxicity, KASP marker, electrochemical performance, precision medicine, thermal criteria, urban wetland, renal function, lead optimization, expression analysis, entropy, biological activity, YOLO26, cell morphology, morphology, P. vulgaris, anchorage system development, phenotypic plasticity, intraocular pressure, diabetes, structured-light metrology, conformational dynamics, 5-HT receptors, antioxidant enzymes, dental implants, extracellular vesicles, acute right heart failure, sarcopenia, post-consumer material, mass loss, polymer gears, LC-ESI-QToF-MS/MS, postharvest, pregnancy, IGF-1, application tests, bonding performance, LLM, systematic review, RNA interference, general anesthesia, hybrid optoelectronic devices, metal partitioning, proteasome inhibitors, endothelial dysfunction, biotic stress, HERVs, Glycine max, silk lutein, molecularly imprinted polymers (MIPs), renal trajectory, stellate cells, statins, medium-density fiberboard, QSPR, ultrasonography, terrestrial ecosystem, multi-target silk bioactives, food packaging, chronic autoimmune thyroiditis, oxygen vacancies, thermal desorption, self-powered devices, silicone oil removal, muscle tone, cardiotoxicity, opioid, proteins, dasatinib, beta cell, hemostasis, reporting odds ratio, SNV, obstructive, Yamane technique, polymer-based formulation, plant-derived bioactive compounds, mitochondria, GlyT2, NaV1.8, fat distribution, concentrated solar power, complications, structural decoupling, genetic disorders, overview, iridotomy, CIP2A, siRNA, cytotoxic activity, artificial intelligence, grain refinement, mass spectrometry, cultivar-specific response, thermal barrier, anthropogenic disturbance, undercarboxylated osteocalcin, PRP, management, cytokinins, amplicon metasequencing, ultrasonic stability, lithium-ion battery, genome-wide association study (GWAS), clinical trial, crumb rubber modified asphalt, postural orthostatic tachycardia syndrome, anemia, ultrasound elastography, PPARα, idiopathic pulmonary fibrosis, gastroesophageal reflux, high-speed aircraft, photoelectrochemistry, surgical navigation, trauma care, compatibilizer, wettability alteration, Damage Control Resuscitation, Ochratoxin A, desorption agent, osteosarcopenia, regular physical exercise, ZIF-8, carotid body tumor, biomedical polymers, poultry, synergistic effects, COVID-19, hormone metabolism, VEGFR-2 inhibition, molecular responses (EMR, responsivity, personalized medical devices, compressive residual strength, benzydamine, GmVTC1, qPCR, chronic inflammation, epiblepharon, stitched composite laminate, oxidative DNA damage, wastewater, CMK-3, YOLO-based visual inspection, target validation, pharmacovigilance, strong-gluten wheat, dual-stage modification, correlation analysis, treatment-free remission (TFR), vitamin K-dependent carboxylation, lymphadenectomy, treatment failure, battery safety, parameter optimization, postpartum, experimental study, cardioprotection, photosynthetic pigments, shear force, bleeding, NAD+ metabolism, sustainability, cox regression, mouse embryonic stem cells, XRF, packaging, proton pumping, skin aging, patient height, MyotonPRO, hypothermia, newborn screening, marker-assisted selection, algal oil, C4-NAD-ME and C4-NADP-ME species, patient satisfaction, non-steroidal anti-inflammatory drugs (NSAIDs), metabolic homeostasis, DSC-defined thermal activation, capsule, cardiology, EBV, RNA-seq, islet amyloid polypeptide, P3HT:PCBM, image-enhanced endoscopy, conservative treatment, lung–kidney interorgan crosstalk, rare diseases, metabolic syndrome, coagulopathy, hyperekplexia, dental biomaterials, α-glucosidase, cytotoxicity, treatment, scalable emulsification, functional ambulation category, nanocoating, biomaterials for medical imaging, deep sedation, rust fungi, microphase dilation, maize, nutrient imbalance, Peto’s paradox, mononucleosis, statistically more accurate formula, mice, seasonality, molecular dynamics simulation, type 1 diabetes, Polycarpon tetraphyllum s.l., glycine transport, polycystic ovary syndrome, thioredoxin-interacting protein, transfusion risk, BiFeO3 thin films, wood-decay fungi, CAPE, dual GIP/GLP-1 receptor agonist, external validation, gastric cancer, wear resistance, adaptation, Cadmium stress, radiation therapy, digital surgery, cv. McIntosh, 3D printing, observational studies, 6-benzyladenine, cellulose nanofibers, energy coupling, balloon test occlusion, titanium dioxide, microsurgical resection, ecological preservation, colchicine derivatives, epithelial-to-mesenchymal transition, polysaccharides, hypoxia inducible factors, periodontitis, mortality, bone mineral density, histochemistry, phenolic acids, predictor, precision oncology, multilayers, liver function, functional characterization, salt tolerance, resuscitation strategies, Mixograph properties, conscious sedation, nerve transfer, multi-material printing, LaNi5, NAFLD, isothermal adsorption, drylands, polygenic risk score, starch biosynthesis, histone deacetylase (HDAC), density functional theory (DFT), AMPs, polyetheretherketone, genetic diversity, coronary reimplantation, bone marrow burden, low-level laser therapy, macular edema, microRNA (miRNA), preoperative embolization, waste valorization, brain slices, degradation kinetics, manufacturing-induced defects, auto-delineation, life cycle assessment, mineral materials, TRPA1, nephron-sparing surgery, photosynthesis, AI-agents, paeoniflorin, shoot culture, cytokinin, brain aging, time series model, carbon nanotubes (CNT), hormone signaling, patch clamp, protein folding rates, heme oxygenase 1, electromyography, pancreatic lipase inhibition, low-velocity impact, WRKY, self-assembly, hemophilia B, biomarkers, heart failure, alkali-activated material, neoadjuvant chemotherapy, cold stress, cardio-oncology, heat tolerance evaluation, pain predictors, DNA repair, dental sedation, thyroid function, HDL–BMI, metabolomics, trauma, selection signature, drug compounds, gene family, tolerance grade, ammonium bisulfite, reaction kinetics, polyaniline, heat-responsive gene expression, clear cell renal cell carcinoma, fat metabolism, new combination, risk stratification, PCOS, orofacial pain, optical properties, adipose tissue, macrophage polarization, CaH2 reduction, invasive species, strength, silent brain lesions, enzyme replacement therapy, germination indices, molecular modeling, deprescribing, stroke rehabilitation, serum creatinine, borderline IQ, acute coronary syndrome, creatine phosphokinase, LED, biochemical alterations, antagonistic pleiotropy, pancreatic ductal adenocarcinoma (PDAC), carotid ulceration, essential oils, deodorization effect, vegetable sweet potato shoot tip, sequence length, ocular surface inflammation, water splitting, euthyroid sick syndrome, acute pancreatitis, copper, cholesterol metabolism, lead enrichment, treatment planning, shear wave elastography, Suzatrigine, plantation forest, collagen, biological durability, chemical decomposition, placenta, α-Amyl cinnamic aldehyde, inter-operator variability, immune-inflammatory microenvironment, finite element analysis, head and neck tumors, prostate cancer, DESTINY, sorghum, workability, exophthalmos, hydrostatic extrusion, corneal endothelial cells, liver cirrhosis, chronic pain, pediatric kidney transplantation, ARIMA model, clinical research, labor, sigma factor, arthralgia, cardiac sarcoidosis, cardiovascular–kidney–metabolic syndrome, genome-wide identification, tenocyte, physicochemical property, H4C11 gene, tethered homopolymer, LC–MS/MS, hazard ratio meta-analysis, Alchemilla alpina, thermal degradation, temporal dynamics of SASP, paclitaxel, rotator cuff, comparative analysis, pharmacological potential, luting materials, gonioscopy, Senegal, Gestational Diabetes Mellitus (GDM), wheat, transcriptional regulation, Spearman correlation coefficient meta-analysis, organic photodetectors, hole electrospinning, two-stage heating sequence, HNP, prediction model, endometriosis, chronic kidney disease, polymer compatibility, coronary button, abscisic acid, perioperative care, NaV1.7, photobiomodulation, cisplatin, spasticity, kinetin, cervical metastasis, organophosphorus pesticide removal, urea–metabisulfite, Teflon felt, large language models, composite foam electrodes, children, autoimmune diseases, tumor therapy, depression, anterior chamber, pressure pain threshold, lignin, LED lighting, thyroidectomy, HCN, synaptic plasticity, phenolic compounds, polysaccharide, emergency medicine, athletes, seed development, geraniol, piglet model, shock management, cataract surgery, TiCN, offspring, eyelid speculum placement, low-molecular-weight organic acid, elevated temperature, siRNA drug nanoparticles, biodegradation, Tessadori–Bicknell–van Haaften syndrome 2, grain quality, hypercalcitoninemia, chronic obstructive pulmonary disease (COPD), perforator method, root traits, customized crack propagation sensor, Saxony, defensin, range shifts, anthracyclines, rice, GA3, radiotherapy, doxorubicin, Brunnstrom recovery stage, visible light communication, structure–function relationship, fish oil, meta-analysis, orphan window, hyperandrogenemia, high temperature resistance, cheating, dissociation behavior, QSAR, K+/Na+ ratio, tumor microenvironment, treatment outcome, liraglutide, PRDX6, colchicine, duplex ultrasound, ND2, composite, isoflavonoid biosynthetic, formaldehyde scavenger, antioxidant defense system, defect taxonomy, SASP score/EGS, risk prediction, Crohn’s disease, detoxification, Africa, nateglinide, outcome assessment, analogue, protein crosslinking, multimorbidity, MAPK pathway, mitochondrial membrane potential, desert ephemerals, intact periodontium, atherosclerosis, heart arrest, deep coal-bed gas, population differentiation, Danio rerio, functional outcomes, root dentin, Th2-M2 responses, exertional rhabdomyolysis, post-marketing safety, cross-species genomics, extracorporeal membrane oxygenation, lipid metabolism, heat-shrinkable polymer, PETase, GAP-43, frictional drag, thermoplastic PP–CF and PP–GF UD composite tapes, green chemistry, dissipative particle dynamics, Ebola virus, cognitive decline, block copolymer/homopolymer blends, case report, intellectual disability, venous congestion, androgens, Brassicaceae, surface topography, fire service, SIRT1, breast phantom, desmosine, UTUC, table olives, tirzepatide, DFT calculations, light intensity, neuroprotection, climate change, ion homeostasis, quality of healthcare, monosaccharide composition, chitosan, supramolecular structures, abstinence, cytokines, monitoring instrument, tonsillitis, primary care, endocrine signaling, confocal laser endomicroscopy, mechanized harvesting parameters, simultaneous thermal analysis, therapeutic drug monitoring, skin fibrosis, frozen section, γH2AX, EudraVigilance, single-cell RNA sequencing, MMP2, metabolic disease, protein engineering, body mass index, activation energy, cesarean delivery, GEN1, chloride impurity, lipids, antioxidant activity, cell migration, combustion characteristic, mutualism, MD simulation, saline-alkali soil, perovskite photodetectors, mitochondrial dysfunction, patient motivation, soybean, conventional properties, chewing efficiency, machine learning, multimodal imaging, lamellar swelling, vacuum assisted delivery, spatiotemporal evolution, T cell differentiation, hemorrhage control, hormones, muscle, sleep apnea, microphase separation, fungicidal activity, visual outcome, false negatives, insulin resistance, bermudagrass, immune system, natural biocide, fibroblast subpopulations, lipid peroxidation, tissue-mimicking materials, paraganglioma, ZrCN, oxidative phosphorylation, telerehabilitation, estrogen receptor, active packaging, hyaluronic acid, thermal stability, public health, radical nephroureterectomy, orthostatic hypotension, biomarker, drought, neck dissection, out of hospital cardiac arrest, hyperalgesia, non-cooperative patients, GH deficiency, hot plate test, herbal medicine, crinkle leaf disease, water sorption, MRI, solar fuels, transfusion therapy, renal replacement therapy, perivascular adipose tissue, oxygen evolution reaction, fatty acid, ulcerative colitis, robot-assisted gait training, analytical chemistry, electromagnetic interference shielding, morphogenetic apoptosis, Riedel’s thyroiditis, bioenergetics, coating, enzyme inhibition, myo-inositol, hemophilia A, determination, linoleic acid, naproxen, inflammation, volatile organic compounds, aortic root surgery, blood–testis barrier (BTB), venous thromboembolism, molecular mechanism, breast cancer, Sertoli cells, sensors, miR-4516, transcriptomics, acute cor pulmonale, patient blood management, composites, mitochondrial signaling, programmed cell death, hybrid bottlebrush copolymer, haskap, crystalline rock, crosslinked polyethylene (PEX), nutrient uptake, MIKC-type MADS-box, oncological surgery, polymer adhesion, acute kidney injury, endometrial organoids, maternal nutrition, light spectrum, NanoBiT, hospital stay, type 2 diabetes, free surface electrospinning, islet cell transplant, lipophilic active delivery, vegetation structure, automotive assembly, polyvinylpyrrolidone, internal medicine, reactive oxygen species, patient-reported outcomes, universal adhesive, macrophase separation, anterior cruciate ligament reconstruction, resveratrol, supraspinatus tendon, human pain, elevated CO2, controlled environment agriculture, α-amylase, Fourier Transform Infrared Imaging spectroscopy, Soybean mosaic virus, electro discharge machining, amyloid, incretin signaling, biopolymer, platelet-rich plasma, HDAC inhibitor (HDACi), network toxicology, glaucoma, PVD coatings, modified silicon quantum dot, PvHAK12, low-energy phase inversion, experimental data deviation, bleomycin, PBAT, milk-derived extracellular vesicles, low molar ratio, recombinant human GH, polyhydroxyalkanoates, graphene, temporomandibular joint disorders, high temperatures at initial grain filling, homologous recombination, prenatal exposure, phase diagram, lipid-rich plaque, elastase, Polycarpon prostratum var. littorale, neural network, autoimmune thyroiditis, fiber post, pancreatic steatosis, ubiquinone, rhesus macaques, local drug delivery systems, doe study, nerve action potential, fatigue and relaxation performance, calcitonin, failure criteria, phase field method, clinical consensus, MM/PBSA, suitable harvest stage, pain therapeutics, acetylcholinesterase inhibition, zinc oxide, ibuprofen, neuron differentiation, aquaporins, velaglucerase alfa, single-nucleotide polymorphism (SNP), norfloxacin, plateau iris, dipeptides, nanofibers, Delphi methodology, CsPbBr3 perovskite nanoparticles, non-human primate, cochlear nucleus, irreversible damage, anti-microbial therapy, vaginal intraepithelial neoplasia, neoplasms, neutrophil-to-lymphocyte ratio, Bacillus, thermal insulation materials, anxiety, epigenetics, C3 species, GPRC6A/GPR158 pathways, specular microscopy, quality, molten carbonate salt, urea–formaldehyde resin, curing behavior, orthodontic movements, immune checkpoint inhibitors, respiratory infection, zinc-manganese flexible battery, pediatric diabetic ketoacidosis, diagnosis, bandwidth, azo dyes, corrosion, renewable materials, LY3526318, hydration characteristics, interdisciplinary management, hobbing, valorization, activating mechanism, thermal runaway propagation, adaptive tooling, Pistacia lentiscus, subacromial bursa, clinical decision support, aldo-keto reductase, fetal growth restriction, Hypericum amblysepalum, denture base resin, maltose-binding protein, hydrothermal carbonization, Parkinson’s disease, polyvinyl chloride (PVC), hyaluronidase, large-scale productions, valence reconstruction, nanoemulsion, fentanyl, mechanical properties, PspA, monoterpenoids, hierarchical porous structure, tear film homeostasis, ligamentization, fragment-based drug design, carbon-fiber-reinforced polymer (CFRP) cables, analytical validation, blood products, trans-cinnamaldehyde, SNAI2, personalized therapy, strain imaging, evolutionary paradox, ketum, baloxavir, GmCSN5B, web server, estimated glomerular filtration rate, natural product, special care dentistry, cardiac disease, multiple myeloma, modified Bentall procedure, PLA, periplasmic translocation, semaglutide, comparative oncology, nondestructive investigation, aging, trabecular meshwork, XRD, p-toluidine-based thiazolidinone-quinoline hybrids, MR2 and MR3), dry eye disease, corona treatment, Gaucher disease, Fe2O3 nanoparticles, natural high temperatures, acoustic indices, asymptomatic carotid stenosis, cv. Húsvéti rozmaring, quarter thaler, CNV atlas, Chlamydia muridarum, IGFBP7, SREBP-1c, physicochemical stability, SARS-CoV-2, fig-fig wasp interactions, computer-aided drug discovery (CADD), DiGeorge syndrome critical region 8 (Dgcr8), mitochondria-associated endoplasmic reticulum membranes, cinnamic series, potassium uptake, T1–T2 N0, ribosomal subunit, taste stability, SLAB51, microstructure, continuous venovenous hemofiltration, response contour plot, obligate associations, human genetics, c-cell hyperplasia, cardiorenal syndrome, nutritional vulnerability, RTK-RAS pathway, severity, meibomian gland dysfunction, electrochemical deposition, apoptosis, knee stability, imiquimod, allosteric regulation, exosome, AKI–CKD transition, NanoBRET, metal hydrides, benzoic series, photoelectrodes, poly(ethylene terephthalate) hydrolase, peri-implantitis, Chlamydomonas reinhardtii, osteoporosis, R-BoT Plus, differential gene expression, Oberlin, nitrogen application rate, soundscape ecology, fatty acid metabolism, bioeconomy, unfolded protein response, residence time, failure, obesity, bisphenol A, ascorbic acid (AsA), cardiac rehabilitation, involute gears, medicinal plants, model validation, oxidative stress, identification, alpha-synuclein, Kaplan–Meier, lifestyle medicine, salt stress, PC12 cells, susceptibility, women’s reproductive health, SPIRIT2, autophagy, risk factors, gemcitabine, male population, reciprocal transplant experiment, additive manufacturing, Iris pumila L., copolymacromer, chronic histiocytic villitis, airway obstruction, hydrodistillation by-products, field application in civil infrastructure, plaque vulnerability, extended reality, EBOV-GP, autonomic medicine, poly(methyl methacrylate), bio-based coatings, infertility, oral cancer, mitragynine, molecular docking, antinociception, vascular smooth muscle cells, LFT-D, ARPIs, narcotics, NGAL, delamination damage, real-world data, molecular imaging, BCL-XL, structural modification, gut microbiota, oxy-fuel combustion, endoplasmic reticulum stress, cardiopulmonary resuscitation, DNA methylation age, mineral composition, hyperlipidemic rat, sugarcane bagasse waste, multi-omics, candidate gene, indoxyl sulfate, PROS1, ferroptosis, metabolic reprogramming, MCF-7, grain size, X-ray single crystal structures, VX993, ubiquitin proteasome system, thermal modification, and prevention
License Label:: Attribution 4.0 International
ORCID:: https://orcid.org/0000-0003-1789-4197, https://orcid.org/0009-0009-5991-9779, https://orcid.org/0000-0002-3603-6414, and
Other Affiliation:: Department of Integrative Translational Sciences, Beckman Research Institute, City of Hope, Duarte, CA and
Person:: Carranza, Francisco G., Waldrup, Brigette, Velazquez-Villarreal, Enrique, Manjarrez, Sophia, and Diaz, Fernando C.
Publisher:: Multidisciplinary Digital Publishing Institute (MDPI)
Rights Statement Label:: In Copyright

33. Orthodontists’ Perceived Knowledge, Confidence, and Clinical Practices in Pediatric Temporomandibular Disorders

Title Tesim:: Orthodontists’ Perceived Knowledge, Confidence, and Clinical Practices in Pediatric Temporomandibular Disorders
Creator:: Southern, Thomas, Sangalli, Linda, Marando, Calli A., and Sawicki, Caroline M.
Date of publication:: March 25, 2026
Abstract Tesim:: Background/Objectives: Temporomandibular disorders (TMD) are common in pediatric patients, yet limited data exist on orthodontists’ knowledge, confidence, and clinical practices related to pediatric TMD. This cross-sectional study aimed to characterize orthodontists’ perceived knowledge, confidence, training, and practice patterns, and examine associations between routine screening behaviors and perceived confidence. Methods: A 34-item anonymous survey was distributed to orthodontists and orthodontic residents enrolled in or graduated from U.S. Commission on Dental Accreditation (CODA)-accredited programs. The survey assessed perceived knowledge, confidence in screening, diagnosis, and management of pediatric TMD, adequacy of residency training (on 0–10 numerical rating scale), frequency of routine TMD screening and examination practices, and referral patterns. Respondents were compared in study outcomes according to years of clinical practice with ANOVA. Respondents were categorized according to frequency of TMD screening (always/some of the time vs. sometimes/never) and compared in study outcomes using independent t-tests. Results: Out of 83 respondents, perceived knowledge (56.8 ± 26.9), confidence with screening (62.0 ± 30.5), diagnosis (59.4 ± 29.8), and management (50.8 ± 30.9) of pediatric TMD were moderate. Less than half of respondents (45.8%) reported routinely screening pediatric patients using standardized screening questions. Orthodontists who reported routine screening demonstrated significantly greater perceived knowledge and confidence in screening, diagnosis, and management compared with those who screened less frequently (all p’s ≤ 0.018, effect size between 0.57 and 0.78). Greater use of specific history-taking and clinical examination components was also associated with higher perceived confidence (all p’s between 0.001 and 0.046, effect size between 0.53 and 1.01). Confidence differed by years in practice, with lower scores reported among residents and mid-career practitioners (p < 0.05). Conclusions: Variability exists in orthodontists’ perceived knowledge, confidence, and clinical practices regarding pediatric TMD. Routine screening was associated with greater perceived competence. These findings highlight potential alignment between structured screening behaviors and self-reported confidence and may inform educational strategies in orthodontic training. and Highlights What are the main findings? <ul> <li>Fewer than half of orthodontists reported routinely using validated screening questions for pediatric temporomandibular disorders (TMD) and overall perceived knowledge and confidence were moderate.</li> <li>Routine use of structured TMD screening was independently associated with significantly higher perceived knowledge and confidence in screening, diagnosis, and management.</li> </ul> What is the implication of the main findings? <ul> <li>Standardized pediatric TMD screening may function not only as a diagnostic tool but also as an educational scaffold that reinforces clinical reasoning and provider confidence.</li> <li>Enhancing orthodontic training and continuing education aligned with the Diagnostic Criteria for TMD pediatric recommendations may reduce variability and support earlier identification and appropriate referral of pediatric TMD.</li> </ul>
Resource type:: Article
Affiliation Label Tesim:: School of Dentistry, Department of Orthodontics, and Department of Pediatric Dentistry
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/h411-2n56
Edition:: Publisher
Identifier:: PMCID: PMC13115295, PMID: 42073022, and DOI: https://dx.doi.org/10.3390/children13040445
ISSN:: 2227-9067
Journal Issue:: 4
Journal Title:: Children
Journal Volume:: 13
Keyword:: subcellular localization, apoptosis, gene rearrangement, metabolic dysfunction-associated steatohepatitis, complicated appendicitis, stress, parasitic infection, serotonin, longitudinal panel, antimicrobial resistance, positive predictive value, Opioid use disorder (OUD), liposomes, bipolar disorders, combination therapy, threat perception, colchicine, emotional embodiment, Na+/H+ Exchanger 3, OsPP2C61, glymphatic system, obturator hernia, transcriptional response, moderately preterm, Thrombosis, wild mushroom distribution, latent class analysis, gut microbiota, serine protease inhibitors, cross-protection, well-being, prolotherapy, rituximab, tumour microenvironment, basal ganglia, positivity assumption, Molecular Biology of Disease, Cardiopulmonary risk, disproportionate reporting, TASK, sustainable nanotechnology, organizational psychological ownership, Sweden, Endovenous ablation, autism, needlestick injuries, TIM-1, polysaccharide nanogels, bio-based nanomaterials, preventive dentistry, ICGN, dead zone compensation, Faeces, sleep disturbances, Candida albicans, antioxidant defense, advanced packaging, cell cycle, Peripatopsidae, PRRSV, C. elegans, icthyosiform mycosis fungoides, Gegen Qinlian Decoction, anomaly detection, tau protein, odontogenic abscess, amebiasis, electroless copper plating, Pediatric cancer, maltreatment, employment discrimination, progress in application, ocular barriers, anti-infective drug discovery, Aboriginal and Torres Strait Islander, cirurgia, alkaline electrochemistry, Systemic vascular resistance (SVR), children, primary care, malaria, resistance mechanisms, GWAS, squamous cell carcinoma, interfacial water structure, user experience, Childhood Cancer Research Network, global linearized sparse prediction, long COVID, CRISPR–Cas, smoking, accuracy (trueness and precision), minimally invasive surgery, pain medicine, metabolic dysfunction–associated steatotic liver disease, DNA ligase fidelity, ophthalmic drug-delivery, VEGF, safeguarding, single-molecule detection, core active component set, bioinformatics, transparency, Virtual reality exposure therapy, dopamine receptors, childhood trauma, pathology foundation model, monitoring, head posture, reddit, Mouse Embryonic Stem Cells, lignocellulosic nanomaterials, drug safety, matrix metalloproteinases, Haemorrhage, life course, multitarget pharmacology, reproducibility, psychotherapy, genetic diversity, Female-prone cardiac concentric remodeling, growth factors, temperature compensation, mammary gland, Metastasis, urinary incontinence, bioelectronics, Calvert formula, glycoproteomics, case–control studies, panic disorder, single-cell RNA sequencing, sex steroids, pagetoid reticulosis, blood stasis syndrome, Membranes & Trafficking, uridine modification, flexible electronics, protein degradation, milk somatic cells, quality by design, autism spectrum disorders, drug compounding, thermal conductivity, Lean body mass (LBM), serpins, piezoresistive pressure sensor, polysaccharides, genomic alteration prediction, transcriptomic, People with lived experience (PWLE), therapeutic proteins, timing, Scenedesmus sp., Shandong province, red deer, root canal disinfection, shear strength, CD274 (PD-L1), PRRSV-2, extracellular vesicles, traumatic brain injury, Sex dimorphism, metabolic biomarkers, Opisthorchis felineus, data fusion, Molecular docking, chlorhexidine, Adaptive behavioral intervention, pediatric practice, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, HMGB1–RAGE, macrophage polarization, edge computing, signaling regulation, renewable resources, intestinal barrier, hypersensitivity, mitochondrial genome, change mechanism, adverse childhood experiences, rare cardiac diseases, chitosan, hematopoietic stem cell transplantation, clustering, microwave ablation, laparotomy, Meta-analysis, lipid peroxidation, Transmission model, development trend, autoimmune cardiopathy, ischaemic heart disease, healthy eating, Post-trauma follow-up, RBM20, dental restoration failure, hypoxia, CETP, microbiome, VEGFA, platinum surfaces, Finger dislocation, preclinical studies, human dendritic cell, deterministic lateral displacement, polyphosphoesters, recurrence, precision medicine, dog, sacrocervicopexy, Riesgo cardiopulmonar, Socioeconomic status, quercetin, digitization of emotions, Enfermedad cardiovascular, bioaccessibility, pain assessment, echocardiography, handgrip asymmetry, coping strategies, boroxazolidones, psychological capital, median sternotomy, Immunomodulation , precautionary principle, HPLC-UV, toxoplasmosis, TGR5, tryptophan metabolism, calcium-ion batteries, Appropriate use criteria, Infection prevention, online health information seeking, Cardiovascular disease, Panarthropoda, workplace harassment, mutation, intraoral scanners, embryokines, thoracic surgery, biodiesel, electroporation, moral intuition, mental health, food-medicine homologous formula, thyroid disease, exercise, Southern Italy, Medicaid expansion, candidate major genes, developmental and cultural perspective, dimensional psychopathology, standing wave, causes of death, functional disability, recombination, skeletal muscle, phase transitions, γ-PGA synthesis, dental caries removal, Nursing staff exposure, women, pulmonary hypertension, Brucella canis, ATPase associated with diverse cellular activities, cattle, poikilodermatous mycosis fungoides, lung cancer, vaping, T-cell mediated, Bayes’ rule, emotional intelligence, college English teachers, heterologous immunity, pair bonding, Generative modeling, smart hydrogel, efficient AI, adverse drug reaction, acetylation, Connexin-43 modulator, lateral flow strip, vascular remodeling, molecular identification, SUPTRS block grant, thymoma, dental caries, phytosome, hypogonadism, neurodegeneration, COVID-19, digital wellbeing, TNM, acetonitrile, preschool teachers, Oral exercise, Oral health, Arterial elastance, PEDV, Ixodes scapularis, Psychotherapy, protozoa, immune modulation, FLNC, non-melanoma skin cancer, upper or middle thoracic esophageal cancer, DTMOS, Biodiesel, Bluetooth low energy transmitter, comparative genomics, notoginsenoside R1, Usher syndrome, health anxiety, drug delivery systems, lithium, Atención multidisciplinar, flavourings, valvular heart disease, adolescent, cardio-rheumatology, venous thromboembolism, congenital disorder, bovine milk, Patient–nurse interaction, molecular mechanisms, skin graft, tricuspid valve, Scaphoid, sex differences, dyslipdemia, third-party punishment, PRRSV-1, oral health, charge transport mechanisms, Neuroinflammation, ritual, tumor-tropism therapy, granulomatous slack skin, transfection efficiency, biomolecular condensates, multi-omics, temporomandibular osteoarthritis, ultrasounds, neurocysticercosis, myeloproliferative neoplasms (MPNs), protein quality control, abdominal aortic aneurysm (AAA), nephropathy, ketamine, machine learning, QuPath, Hematopoietic Stem and Progenitor Cells, temperature variation, network meta-analysis, Dendrobium, feces-induced peritonitis, lipid nanocarriers, mycosis fungoides, lipid nanoparticles, eosinophil count, matching control, magnetophoresis, Onychophora, pediatric, trazodone, psychological reserve, variant interpretation, exploitation, PTSD symptom severity, cardiovascular mortality, E-cadherin, pulmonary embolism, family work conflict, biostatistics, tratamiento, prediction intervals, gender identity, esophagectomy, metabolic syndrome, lichen planus, vascularized composite allotransplantation (VCA), Oral frailty, co-culture, frailty, school bullying, testosterone, ecological study, tissue engineering, Parkinson, gene pathways, β/ε subunits, Enterococcus faecalis, Seymour fracture, scoping review, oxidative stress, ocular bioavailability, X-ray-induced photodynamic therapy, lactation, SCFA, ethnobotany, correlative factors, protein conductance, kidney disease, ring VCO, locomotive syndrome, Renal Fanconi Syndrome, Unidades cardiopulmonares, pathological motor features, work meaning, cardiomyopathy, age-standardized death rate, clonal hematopoiesis of intermediate potential (CHIP), middle cerebral artery velocity, triboelectric nanogenerators, acyl-CoA carboxylase, kidney, memristor emulator, wild mushroom intoxications, SEGE index, Nutrition, cortisol, engineered exosome, polymorphisms, therapeutic potential, internal psychological control, peduncle length, intubation time, DNA Replication, Recombination & Repair, OXPHOS, anterior segment, systemic inflammatory indices, DNA–protein interaction, helminths, Acromial stress fracture, maxilar, Chronic obstructive pulmonary disease, functional nucleic acids, CRISPR/Cas12a, flavour ban, Hallucinogens, angiogenesis, Bipolar disorder, relationship type congruence, e-cigarettes, kinesin inhibitors, methionine oxidation, physical health, model predictive control, cyberchondria, Well-differentiated neuroendocrine tumors, tratamento, MRI, Reverse shoulder arthroplasty, deep venous thrombosis, disability, Alhagi maurorum, protein losing nephropathy, student reflection, Lingguizhugan decoction, variant allele frequency (VAF), antidiabetic effect, earthquakes, phylogeny, endobronchial ultrasound, low temperature, straw biodegradation, inpatient care, LGBTQ+ employees, chondrocyte, Multidisciplinary care, spatiotemporal expression, artificial intelligence, K2P, acute appendicitis, Plasma cell pododermatitis, postattenuation seizures, catheter duration, acute leukemias, thermal conductive silicone adhesives, surgery, SARS-CoV-2, urticaria, heterocyclic compound, Healthcare worker safety, centrum semiovale, Tumor microenvironment, bootlegging innovation behavior, GPCR, wound healing, Extracellular matrix, in vitro environment, comorbidity, mortality, diabetic nephropathy, stressful events, ICD, meat quality, firefighters and emergency medical responders, wearable electronics, type 2 diabetes mellitus, Varicose veins, CTG repeat polymorphism, Catalysis, adverse events, agent, social relationships, lifecycle assessment, thematic analysis, kidney transplantation, Mechanical signaling, AI literacy, prion-like domain, therapeutic resistance, blastocyst, metal-induced oxidation, Serotonin 5-HT2A receptor, corticosteroids, odontogenic, crRNA, thoracic aortic aneurysm (TAA), LMNA, safety-engineered device, chronic kidney disease, high load resistance training, whole-exome sequencing, maxilla, ARTP mutagenesis, multi-attribute method, HIF-1α, sexual dysfunction, immunosuppression, transgender employees, Outcome, bystander intervention, peptide mapping, multi-drug resistance, epidural catheter, angle classification, retinal dystrophy, ALS, postoperative complication, Anticoagulation, oligosaccharides, NF-κB signaling pathway, HALP score, nebulization devices, warm-needle acupuncture, education, repeat visits, population pharmacokinetic model, self-powered sensors, late preterm, CRP, schistosomiasis, Sustainability, de novo urinary incontinence, adaptive expertise, fibrinogen, surgical ligation, chromosome, ischemic priapism, Euglena gracilis, non-12-OH BAs, FAERS, gut–liver axis, DEGs, sustained-release device, treatment, cutaneous T-cell lymphoma, GIDL erase, maxila, thermogenesis, Lactiplantibacillus plantarum, biorelevant dissolution, Community engagement, lecithin-based quercetin formulation, nitrogen upcycling, G protein-coupled receptor (GPCR), knife crime, mHealth engagement, gutta-percha, sharps injuries, diffuse coronary artery disease, clinical translation, plant-based biomaterials, minimally invasive surgical procedures, early neonatal mortality, qualitative analysis, psychiatric outcomes, JAK2 V617F, blood pressure, workplace mistreatment, epigenetic regulation, AI integration, LC-MS, bronchoscopy, immunomodulation, phylogeography, enlarged perivascular spaces, transcriptomics analysis, lymph node staging, healthcare utilization, polyphenols, rice (Oryza sativa L.), hnRNPA1, youth justice, fibrillation, whole-slide imaging, Australia, public health policy, membrane interactions, violence, kinase inhibitors, mixotrophy, Streptomyces coelicolor, ESKAPE pathogens, laser-induced transfer, adaptive Kalman observer, inflammation, malocclusions, victims’ coping strategies model in China, nodular onchocercosis, ab initio moleculardynamics, mitochondria, sepsis, endodontic biofilm, Opisthorchis viverrini, sustainability, cardiac MRI, province, debris sorting, wireless telemetry, family, Clostridium tertium, sternal closure, Levy classification system, carnosine, circumcision, healthcare system, affective symptoms, Transesterification, obicetrapib, papaverine, hard gelatin capsules, tooth, electrochemical double-layer, Close contact, mixoma, bacteriocin, Antipsychotic drugs, Liver fluke, deterrence theory, Botanical fungicide, regression, DNA, biomarkers, cirugía, side-effects, gene network, Cardiopulmonary units, pig, supraclavicular lymph node dissection, antioxidant enzymes, cholesterol, DNA methylation, polymorphism, Phytophthora parasitica, meta-analysis, physiotherapy, ginkgetin, post-weaning diarrhea, genetic susceptibility, phimosis, schoolchildren, Transcription Regulation, gene expression level, deciduous, social connectedness, catheter-related infection, urban health systems, SDS-PAGE, balneotherapy, television, Decision making, low back pain, diabetes mellitus, omics, post-transplant cyclophosphamide, ezetimibe, external psychological control, Pediatric hand, socioeconomic inequalities, glycolysis, gene mapping, sacrocolpopexy, Large White pigs, serum sickness, mitotic regulators, Cercospora beticola, Clonorchis sinensis, molecular epidemiology, Xuefu Zhuyu Decoction, Modified Slime Mold Algorithm (MSMA), hydrogenunderpotential deposition, ventricular arrhythmias, traveling wave, LLPS, outcome-wide, immunosuppressive therapy, emotion regulation, non-HDL cholesterol, Older adults, TMTC enzymes, proteinuria, Brix 3000, MEMS, Children’s Oncology Group, homelessness, 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prediction models, carnosinase, dual-mode oscillator, bystander responses, vinblastine, critical thinking, two-stage sorting, tri-subunit complex, Stroke, oocyte maturation, breast cancer, steroid hormone biosynthesis, non-redox proteins, systemic immune-inflammation index (SII), meteor craters, mechanical interlocking, sudden cardiac death, electrochemical impedance spectroscopy, mechanism of action elucidation, malignant biliary obstruction, viral evolution, Boron, Chromatin, Transcription & Genomics, RNA-seq, double-layercapacitance, beef marbling, DNA ligation, quorum sensing, hydrogels, biomedical signal conditioning, Sorghum, health personnel, plant extracts, adolescence, non-neutropenic host, pelvic organ prolapse, Enfermedad pulmonar obstructiva crónica, reverse transcription recombinase-aided amplification, syringotropic mycosis fungoides, cardiometabolic risk, computational pharmacology, peroxisomal disorders, social behavior, pediatric immunology, ultraviolet C, nicotine, chemotherapy, cognitive impairment, metabolomic, autoclaving, GC skew, Onchocerca flexuosa, nanomaterials, temporomandibular disorders, suicidal ideation, psychiatric comorbidity, hypopigmented mycosis fungoides, sleep health, cholangiocarcinoma, Cell Fate Transitions, seroprevalence, Drug utilization study, tick feeding, TWIK, biosecurity, Alzheimer’s disease, leishmaniasis, Epigenomic Remodeling During Differentiation, ANGPT2, Arteriviridae, Plant–pathogen interaction, writing assignment, abuse, Surgical management, late neonatal mortality, Darwinian evolution, Peripatidae, Intervention strategies, empathy, anaphylaxis, physical activity, Japanese Black cattle, FLIM, digital dental impressions, swine, IgG4-related disease, lyophilisation, Sustainable agriculture, molecular sieve-assisted drying, New Zealand, 3D NAND flash memory, LDH, nanocarriers, Proximal Tubular Cells, glomerular disease, dose administered, friendship, late gadolinium enhancement, GMP scale-up, orthodontists, sociology of emotions, Cystinosis, carnosine metabolism, Mannheim variant, computational pathology, folliculotropic mycosis fungoides, quality of life, natural products, reassurance-seeking behavior, motor disturbances, cardiometabolic risk factors, Fibromyalgia, cell on peripheral, occupational safety, diabetic kidney disease, posttraumatic stress disorder, intra-abdominal infection, piezoelectric actuator, electrolyte dehydration engineering, safety, mistreatment prototypes, Computer assisted grading, kynurenine pathway, Hand trauma, atherosclerosis, Diabetic macular edema, frequent attenders, direct laryngoscopes, indirect laryngoscopes, Prevalence use, facial transplantation, public behavioral health systems, age-related trends, Parkinson’s disease, laparoscopic, paediatric, cellular disruption, desmoplakin, buccal cells, phenotype-genotype correlation, respiratory toxicity, Heimler syndrome, eGFP mRNA, CD variation, discrimination, Knee osteoarthritis, pigeon rotavirus A, sorting, desmosomal genes, probiotics, aging, high volume resistance training, carboplatin, Huntington’s disease, biological sciences, Cardiac elastances, Bacillus velezensis GY1, job burnout, Ki-67, immune complex glomerulonephritis, photo-dynamic therapy, origin of life, CNDP1, pneumoperitoneum, neurotoxicity, cognitive frailty, supported lipid bilayers, procalcitonin, occlusal plane, myxoma, multivalent ion electrochemistry, pediatric dentistry, cardiovascular diseases, PEX6, O-mannose glycosylation, Microporous materials, year, DNA enzyme, magnesium, instability index, Mechanotransduction, affinity proteomics, LUSMs, reaction to Dacron material, microwaves, artificial oxidation, hemiplegia rehabilitation, in vitro gastrointestinal model, neutrophil-to-lymphocyte ratio (NLR), cutaneous malignancy, trypanosomiasis, consistency, aggregation, attachment style, malignancy, authentic emotional displays, neutralizing antibodies, epidural port, Lung neoplasms, Finger fracture, FiberWire, IFAT, ligase fidelity, cardiac pacemaker, immune infiltration, sternal fixation, geriatric syndromes, C-reactive protein, immunotherapy, criminal-justice system, nonsteroidal anti-inflammatory drugs, ammonium nitrogen removal, transportation-related activity, immunology, older adults, defense response-related genes, resistance exercise, care systems, embedded system implementation, genotype–phenotype, epidemiology, chirp generator, oophorectomy, social intelligence, green synthesis, register study, postneonatal mortality, and Wastewater
License Label:: Attribution 4.0 International
ORCID:: , https://orcid.org/0000-0002-1386-5594, https://orcid.org/0009-0000-5191-4169, and https://orcid.org/0000-0001-9295-9849
Other Affiliation:: and College of Dental Medicine—Illinois, Midwestern University, Downers Grove, IL
Person:: Southern, Thomas, Sangalli, Linda, Marando, Calli A., and Sawicki, Caroline M.
Publisher:: Multidisciplinary Digital Publishing Institute (MDPI)
Rights Statement Label:: In Copyright

34. Quality of ChatGPT-Generated Responses to Common Patient Questions About Peripheral Nerve Stimulation: A Cross-Sectional Study

Title Tesim:: Quality of ChatGPT-Generated Responses to Common Patient Questions About Peripheral Nerve Stimulation: A Cross-Sectional Study
Creator:: Smith, Christina, Osuagwu, Uzondu, Hsu, Annie W., Siddique, Muhammad Uzair, Adekoya, Peju, Comrie, Frederick K., Odonkor, Charles A., Abd El Sayed, Alaa, Prabhakar, Hari, Pokuri, Sreekrishna, Locklear, Jacob, Paek, Jonathan, Martins, Yuri Chaves, Palaniappan, Sarvesh, and Adler, Alexandra
Date of publication:: 2026
Abstract Tesim:: Background: Peripheral nerve stimulation (PNS) is increasingly used in selected patients with neuropathic pain, and many individuals seek supplemental online information to clarify procedural expectations and postoperative care. Large language models such as ChatGPT may provide scalable patient education; however, their performance for PNS-related questions has not been evaluated. This study assessed the reliability, accuracy, and comprehensibility of ChatGPT-5.0 responses to common PNS patient questions. Methods: We conducted a cross-sectional evaluation of ChatGPT-5.0 responses to 21 standardized questions derived through expert consensus, spanning pre-implantation, implantation, and post-implantation domains. Sixteen board-certified interventional pain specialists and a nurse educator independently rated each response using validated scales for reliability (1–6), accuracy (1–3), and comprehensibility (1–3). Descriptive statistics were calculated, and domain-level patterns were examined. Results: Clinician ratings demonstrated generally strong performance across all domains. Mean reliability was 4.7 ± 1.4, mean accuracy 2.6 ± 0.6, and mean comprehensibility 2.8 ± 0.5. Foundational questions addressing mechanisms, expectations, and postoperative care received the highest ratings. Lower ratings were observed for implantation-focused items requiring procedural nuance. No response fell below predefined acceptability thresholds, and sensitivity analyses confirmed that including one partial evaluator did not alter the observed trends. Conclusions: ChatGPT-5.0 generated responses to PNS-related patient questions that clinicians rated as generally reliable, accurate, and understandable, particularly for foundational and postoperative topics. Performance was more variable for procedural questions, underscoring the need for clinician oversight and verification. These findings provide a benchmark of current LLM capabilities and highlight the importance of ongoing evaluation as models evolve and as patients access versions with differing functionalities.
Resource type:: Article
Affiliation Label Tesim:: College of Arts and Sciences and Department of Biology
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/1tf4-1k33
Edition:: Publisher
Identifier:: DOI: https://dx.doi.org/10.3390/clinpract16040066, PMID: 42041943, and PMCID: PMC13138679
ISSN:: 2039-7283
Journal Title:: Clinics and Practice
Journal Volume:: 16
Keyword:: postspinal hypotension, Tuberculosis, In situ releasing, pulmonary physiology, necroptosis, Cardiogenic shock, Immortalization, Central venous pressure, Survival, Bolivia, data acquisition, Ommaya pump, Oral squamous cell carcinoma, JAK-STAT signaling, Depression, Quality of life, Chronic hepatitis B virus infection, Lysinibacillus sphaericus, aCLL, Risk factor, FibreSimulator, COPD, Newborn neonates, Phosphorus availability, Brain abscess, Reproductive health, Sleep deprivation, Aspergillus fumigatus, aztreonam, Gender-based violence, Analgesic, Decision-tree model, Endoplasmic Reticulum, Prostate cancer, pseudorabies virus, Endonasal endoscopic dacryocystorhinostomy, Extrapulmonary tuberculosis, pulmonary rehabilitation, Ageing, Bloodstream infection, Single-cell transcriptomics, Neutrophil-to-lymphocyte ratio, peripheral nerve stimulation, High altitude, Silicone intubation, Rep_3/OrfX plasmid, Congenital heart disease, NEC, Disease progression, CD70, KAT2A, synchrotron instrumentation, Ketamine and morphine are individually effective analgesics for trauma-related pain. This study confirms that their combination enhances pain control, shows a trend toward reduced opioid consumption, and maintains a favorable safety profile, supporting its use in emergency trauma care., Biomolecular condensates, severe hemophilia A, type 2 diabetes, Rhodotorula toruloides, Cost-effectiveness analysis, Vaginal discharge, Centenarians, neuropathic pain, Osteoarthritis, Superfical zone damage detection, Bulgarian cohort, Dose summation, Time-of-flight mass spectrometer, Early maladaptive schemas, UFMylation, Appetite, Phylogeny, denoising, Logistic regression, Mouse brain delineation, Non-Attendance, Central sleep apnea, depression, serotyping, bacterial cell wall, Mycobacterium avium complex, Caregiving Impact, breast neoplasms, VEGF-C, Neurology, Cesarean delivery, neonatal sepsis, Eating Disorder Examination Questionnaire, Version 6 (EDE-Q), nerve block, Pharmacogenomics, Intervention, Adherence, Virtual reality, Fat fraction, photon energy, Intestinal strangulation, calves, feature weighting, reliability analysis, Emerging, GPX4, postoperative pain, Safety outcomes, Naïve Bayes, chronic inflammation, Associated factors, Reproducibility, Apoptosis, Laying hens, Alzheimer's disease, WHO HEAT, ceftazidime–avibactam, Ki-67, Socioeconomic factors, gut microbiome, research needs, Ecology, Torulene, Catch-up vaccination, c-Myc, antifungal tolerance, PresbyMAX, C2H2 zinc finger protein, Magnetic resonance angiography, azithromycin derivatives, Rhizosphere microbial community, single-source extraction, Inequity, heat value, Gaussian process, Scale Development, Muscle performance, Curative effect, Translation, Fatal outcome, CFS, modified Delphi process, PET/CT, Rhizosphere metabolome, Post-translational Modifications & Proteolysis, Prescription medication use, Cancer-testis long noncoding RNA, forensic radiology, FEV1, health services research, Intention to use, Cancer, reperfusion, beamline endstation, glycaemic control, Qualitative study, Three-dimensional characterization, cystic fibrosis (CF), Validity, Systematic review, Bibliometrics, C-reactive protein, SLC19A1, pdif site, nucleic acid extraction, Biology, Patient-centered outcomes, Medical research, Multiple myeloma, gene dosage effect, Mitochondria-targeted, Malaria transmission model, extracellular signal-regulated kinases 1/2, medial clavicular epiphysis, Trace mineral supplementation, Immune-based combinations, Interstitial, Surgery, antimicrobial agents, Chromatin, Transcription & Genomics, mild cognitive impairment, Hemangioma, Traditional medicine, Image-guided radiotherapy, soil virome, Ribosome Stalling, Antenatal Care, NRCS-A, Chemotherapy resistance, Implementation science, Fish cell line, Human Immunodeficiency Virus, vasopressor administration, Intra-abdominal hypertension, Kidney genomics care, Coinfection, Infant, Research trends, Enzymatic hydrolysis, LPS, ligament tension assessment, Molecular mechanism, Longitudinal analysis, Vietnam, mHealth, monochromator, angular resolution, Neonatal, Metastatic disease, necrotizing granuloma, Membranes & Trafficking, Fatty liver hemorrhagic syndrome, dead hyphae, Canine gastrointestinal disease, Suicide Stem Completion Measurement, Work fatigue risk, T Cell Engager, Genetic kidney disease, innate response, Macrophage, interventional, X-linked inhibitor of apoptosis protein, vertical transmission, inhibitors, BRD, Glioblastoma, Antifungal susceptibility, Sulfur chemiluminescence, Adhesion/ECM, Knee arthroplasty, randomized controlled trial, Vascular malformation, NLRP3 Stability, Palmitoylation, RBBP-5, Transcriptionally earliest state, Proximity labeling, Thrombocytopenia, Leishmania (Viannia) lainsoni, trauma, ARPES, chest computed tomography, Lung adenocarcinoma, Left ventricular assist device, YY1, High-risk clones, unified protocol, Mesenchymal stem cells, rest confirmed, Biomarker, Metastasis, dif module, Maternal–fetal interface, telemedicine, Diet, valence-to-core emission spectroscopy, Virulence factor genes, Suicide risk, stroke, Pain, Citation analysis, Cucumber Fusarium wilt, lung biopsy, adolescents, real‐world evidence, cellulose column chromatography, regression analysis, Diagnosis, high-throughput experimentation, Post-traumatic stress, Intrathecal therapy, fNIRS, Hypertension, Difference, Gender norms, Cumulative dose, Transition window, detectors, First-line registered nurses, Hot spots, Candida, metabolomics, major adverse cardiovascular events, EDHS, Protein-losing enteropathy, Datathon, PD-1/PD-L1 inhibitor, Vein of Labbé, Patient and public involvement, Attention fusion, Gene families, Lower limb fractures, Phrenic nerve injury, Pulmonary embolism, Extracellular vesicle, Multifocal, Ras/ERK pathway, MEK/ERK, Multivariate logistic regression analysis, Anorexia nervosa (AN), Prognosis, Alcaligenes faecalis, ATMPs, plasmid outbreak, Albuvirtide, Invasive fungal infections, Physical function, data analysis, CNTN3, Mixed-effects model, synchrotron, SOCS3, Caregivers, optics, Safety, Treatment response, Immune thrombotic thrombocytopenic purpura, PCR, Vascular anomalies, Ganoderic acid, Household water access, Scenario modelling, early relational health programs, Afghanistan, Clinical trials, Time series, Hypofractionated radiotherapy, organizing pneumonia, Single nuclei sequencing, Prevention, Proteomics, Adhesive small bowel obstruction (ASBO), Idiographic symptom networks, Observational study, Methyltransferases, benzoxaboroles, BRCA1, large genomic deletion, Brain volume, Dose accumulation, beam-positioning monitor, Primary acquired nasolacrimal duct obstruction, De novo lipogenesis, cross-sectional, Psychometrics, Stem Cells & Regenerative Medicine, EMT, Next‑generation sequencing, Recruitment interventions, microRNA, Somatic, artificial intelligence, Oxygen therapy, WDR-5, random forest, Machine learning, shotgun metagenomics, Heart Failure, Stress, Dog, intubation’, tramadol, β-Carotene, Marker-assisted selection, CFA, Dairy cow gut microbiome, Osteosarcopenia, cardiovascular outcomes, Single-cell, Non-Small Cell Lung cancer, virulence, Early Endosome, robotic‐assisted surgery, Brevundimonas diminuta, GLP‐1, RNA processing, rVSV, Pseudomonas aeruginosa, Lung diseases, Inflammatory markers, Structured follow-up, Competency, Tuberculous pleurisy, Infective endocarditis, emission sphere, Presbyopia, Hippocampus, Health literacy, Fluorescence microscopy, Global burden of disease, electronic structure, Multidrug resistance, Nicotinamide riboside (NR), PRRX2, resistance, multi-omics, Diagnostics, pathobiome, comparative effectiveness, Ganoderma lucidum, Contraceptive methods, mortality, Combination therapy, gestational diabetes, emm type, Hepatocellular carcinoma, Mental health, Transdifferentiation, Thematic analysis, Disease surveillance, Transparency, Attachment styles, Privacy budget, Embryonic Stem Cells, vaccination, SPring-8, Mitochondrial unfolded protein response (UPRmt), Prognostic model, post operative nausea and vomiting (PONV), Adult plant resistance, Resistome, Image registration, Latent profile analysis, Older Adults, ST-segment elevation myocardial infarction, Articular cartilage, XAS, Senescence, CETN2, Intensive care unit, group A Streptococcus, Teacher support, NF1, infarct-related artery, postoperative, Divergence, phage tail-like bacteriocins (PTLBs), Ovarian cancer, Cognitive-behavioral therapy, Liver cancer, clinical research, EFA, aminoglycoside phosphotransferase, endogenous CRISPR-based genome editing, MLL/SET/COMPASS complex, Acute kidney injury, Surgical method, Chemotherapy, HIV post-exposure prophylaxis, Secondary metabolism, computed tomography, Esophageal cancer, Weil’s disease, Cell therapy, high-performance computing, Antimicrobial resistance genes, ChatGPT, R- and F-subtype PTLBs, Immunotherapy, Immune, Latent transition analysis, Flow modulation, Metal-organic framework, Children, Ferroptosis inducer, ANZICS, Rab11-FIP2, drug susceptibility, Severity of illness index, Metagenomics, Group therapy, EQD2Gy, ccRCC, Rapid progression, Corneal aberrations, Glycosylation, COVID-19, Germline, Cell-free DNA, Immunology, Health Equity, vaccine, Angioarchitecture, Tanzania, Pex8, Reproductive stress, Data augmentation, Violence against women, Thermal radiofrequency ablation, Streptococcus pneumoniae, APP, energy distribution curve, nocturnal symptoms, Critical care, Therapeutic resistance, Privacy-preserving algorithms, Community, Pediatric viral pneumonia, predictive modeling, SLS 2.0, H3K4 methylation, placenta, Phylogenetics, diffuse alveolar damage, Primary care, Technology acceptance model, Predictive model, Whole-genome sequencing, Chromosome X, Candidemia, immune tolerance induction, translation, X-chromosome Inactivation, NHANES, MU opioid receptor, Cross-cultural, Outcome, medication delivery systems, pre‐excision, Therapeutics, self-management, aerobic exercise, Immune-related genes, chronic obstructive pulmonary disease, Mass spectrometry, female, Lung injury, Chronic spontaneous urticaria, In-service examination, SBRT, Rheumatoid arthritis, inhaler technique, Chronic pain, HDAC, 18F-FDG, Streptococcus suis, Gastric mucosal hypertrophy, Bioactive compounds, morphine, Homologous recombination, Men who have sex with men, Pittsburgh Sleep Quality Index, semaglutide, Quality of work life, LMM, Lignocellulosic biomass, Neutrophilia, Cattle, Bacteriophage, Heterochromatin, phylogenetic clustering, Wheat, Organelles, viromics, Dengue virus, Acetylation, imaging in beam monitor, Inflammation, Open fractures, APX, Traumatic brain injury, Medical education, Kidney tubular cell stress, Immune infiltration, localization, Critical illness, Inflammasome Activation, band structure, trephine biopsy, Male contraception, Radiotherapy, Practice-based intervention, Acetazolamide, Masculinity, multidrug-resistance (MDR) bacteria, Cost-effective analysis, Adolescents, Parental educational anxiety, large language models, angiogenesis, Health, Cefazolin, virus replication kinetics, MPT64 antigen, Vascular, Mendelian randomization, chronic pain, Intensive care units, outcomes, Canine distemper, ketamine, beam profile monitor, Hemoglobin-A1c, Medical data sharing, Biomarkers, brain health, Economic Status, AC007271.3, quasi-elastic gamma-ray scattering, Reactive oxygen species, Pregnant Women, Acinetobacter species plasmids, linkage, transmission electron microscopy, alpha-1 antitrypsin deficiency, clavicle length, IUGR, Ultrasound, ultrasonography, Protocol, Nanobody, Alzheimer’s disease, Diseases, Cell line establishment, Cervical cancer, GNRI, beamline design, conventional radiography, Discogenic low back pain, Biorefinery, emergency medical services, Bone-muscle axis, age estimation, Venous thromboembolism, imageless, aspergilloma, Reirradiation, Childhood trauma, Healthcare professionals, viral particle enrichment, 1.5-stage, Glycation, quality of life, TP53, Vein of Trolard, human microbiome, Autophagy & Cell Death, Estradiol, Gut virome, Job satisfaction, C. elegans embryo, Hepatitis E, Internal carotid siphon aneurysm, Jaundice, Airway management, antimicrobial resistance, Nucleolus, prokaryotic engineering, FAM83D, Inferior anastomotic vein, OPRM1, Support vector machines (SVM), School-aged children, Continuous positive airway pressure, Nephrogenetics, Cardiovascular diseases, Candida blankii, Theory, Stenotrophomonas maltophilia complex, Escherichia coli, rVSVΔG-ZEBOV-GP, Unintended pregnancies, Proteostasis, Immunosuppression, Machine learning algorithms, FGF4, LOS, autoencoder, Immune-related adverse events (irAEs), fungus ball, Early ambulation, Acute respiratory distress syndrome, Candida albicans, Network meta-analysis (NMA), Hospital-based surveillance, sleep quality, Endovascular treatment, isoflavones, Filarial nematode parasite, neuromodulation, Administrative health data, Craving, total knee replacement, 4CP/PheSv counterselection, Pharmacists, Anxiety, blood–brain barrier (BBB), regional, bovine, saliva, daptomycin resistance, Urinary incontinence, Health care, Therapy, cancer pain, 18F-PSMA-1007, Ovarian Cancer, Malaria vaccine, Mortality, Methamphetamine, Unimproved sources, diamond sensor, Streptococcus pyogenes, Comprehensive GC × GC, hard X-ray, machine learning, Superior anastomotic vein, Kidney genetics clinic, soil microbiome, Pediatric cardiac surgery, 2-photon, Self-efficacy, modified radical, virome, teach-to-goal, digital PCR, Efficacy, Perioperative neurocognitive disorders, Quantitative MRI, Clinical Frailty Scale, Heart rate variability, Transcriptome, nursing home, Personalized psychiatry, Claudin-6, Development, Self-amplifying RNA, Neuroimmunology, Organelle-targeted drug delivery, intubating laryngeal tube suction, respiratory morbidity, pediatric primary care, Mesenchymal stromal cells, Genome-wide association studies, miR-206-3p, Metabolomics, Recurrent pregnancy loss, DLEU2, gene amplification, Classification, Treatment efficacy, Evolution, Drug resistance, maternal-fetal health, Septic emboli, Mental Illness, PJI, Amphiphilic properties, Flow diversion, male, X-ray emission spectrometer, Vasectomy utilization, Local differential privacy, IA stage, Platinum drug resistance, intubating laryngeal mask airway, Eating disorders (ED), Multi-organ dysfunction, Multi-team analysis, Aging females, Ferroptosis, and Marker trait associations, Parity-related outcomes, Brain atlas, Graph representation learning, Riemerella anatipestifer, tuberculosis diagnosis, mouse model, waist‐to‐height ratio, Reliability, Hyperbaric oxygen therapy, adults, exercise-induced desaturation, Multiorgan infarction, Ethiopia, Intranasal, severe obesity, dsRNA, pain, aztreonam–avibactam, Cost-effectiveness, Quality of care, Management, This study showed the incidence of NP is high among patients undergoing BST cancer surgery, particularly amputation, pelvis tumors, and plastic reconstructions, with predisposition related to nerve handling and age., verbal fluency task, LPS Priming, Lung-brain axis, therapeutic virus production, Muscle satellite cells, mastectomy, patient education, Methotrexate, Social media, Older adult, Race, emicizumab, Frailty, Platelet-rich plasma, Family planning services, TurboID, gestational hypertension, Transcription factor, Bone soft tissue cancer surgery, Clinical practice, Kidney renal clear cell carcinoma, Parental involvement, Embryonic callus, Academic burden, type 2 diabete mellitus, Sierra Leone, Socioeconomic status, Ribosome-associated Quality Control, Dense segmentation, Anesthesia, phenylephrine, 6-minute walk test, Pregnancy, Veterans, Oxford Nanopore Technologies, Renal failure, Health literacy interventions, Systemic lupus erythematosus, Ischemic stroke, ERα, Hyponatremia, symptom burden, Capacity building, Cost barriers, N-Acetyl-Aspartyl-Glutamate, vesicular stomatitis virus, Ultra depth of field microscopy, Immersion therapy, Health Surveys, Obsessive-compulsive disorder, HMG-box protein, Meningitis, adverse events, CRISPR, foam cell, Chronic disease, Posterior reversible encephalopathy syndrome, Bibliometric analysis, CLE fusion protein, Roasted coffee, Endosomal trafficking, radiography, miR-330-5p, Racial differences, Lung cancer, T2 mapping, Rice, Case report, Epigenetic regulation, Translation & Protein Quality, food frequency questionnaire, Neurodevelopmental, Malaria control, meta-analysis, S. capitis, Multidisciplinary team, colorectal cancer, Eating disorders, Bibliometric, Severe leptospirosis, Chromatin, semi-mechanistic pharmacodynamic modeling, feature selection, Women employees, data reduction, gut microbiota, Neurobehavioral symptoms, catalysis, field-programmable gate arrays, Disease-snoRNA associations, mechanism of action, Molecular typing, Antibiotic prophylaxis, weight control, anoikis, C. elegans, Obstructive sleep apnea, Iptacopan, ECM remodeling, D-dimer, Cardiology residency, SORLA, value-based care, DIA proteomics, Shadow education, PEG, Type 2 diabetes mellitus, Veterinary regenerative medicine, cohort study, CRC, Behaviour change, context specificity, Aortic dissection, Mitophagy, Osteoprogenitor cells, Loa loa, oxygen therapy, Data sharing, macrolides, Reproductive Health, Bangladesh, C─H oxidation, Exosomes, Genetic nephrology, Spacer, Fungal, X-ray absorption spectroscopy, Nomenclature, Rusts, Heroin, Sex, Solid phase microextraction, animal model, antibiotic resistance, Joinpoint Model, Healthcare Disparities, Surgical site infection, Recurrence, Diaphragm paralysis, Neuroinflammation, South Africa, TP53 mutations, Differentiated callus, Agouti-related protein (AgRP), Delirium, Nursing, SIADH, Guidelines, Pathogenomics, Implicit measures, Heterogeneity, inflammation, lipid metabolism, Odor active sulfur compounds in food, thrombectomy, hepatocellular carcinoma, ZDHHC2, Prognostic signature, all‐cause mortality, Oncogenesis, DTT, nebulizer, Perioperative, Clinical decision support, elderly adults, Hospital pharmacy, SynCom, Meningeal lymphatics, APH(3′)-IVb, granuloma, Health care development, and computational modelling
License Label:: Attribution 4.0 International
ORCID:: , https://orcid.org/0000-0003-3395-9085, https://orcid.org/0009-0006-7682-5297, https://orcid.org/0000-0002-8280-4015, https://orcid.org/0000-0003-4220-4979, and https://orcid.org/0000-0002-8285-8785
Other Affiliation:: Department of Orthopaedics and Rehabilitation, Division of Physiatry, Yale New Haven Hospital, New Haven, CT, Department of Pain Medicine, Division of Anesthesiology, Critical Care, and Pain Medicine, University of Texas, MD Anderson Cancer Center, Houston, TX, Department of Anesthesia, Critical Care and Pain Medicine, Division of Pain Medicine, Johns Hopkins Hospital, Baltimore, MD, Department of Rehabilitation Medicine, Trinity Health of New England, Hartford, CT , Department of Anesthesiology, University of Wisconsin, Madison, Wisconsin, WI, Aviator Pain & Spine, Grovetown, GA, Department of Anesthesiology and Pain Medicine, Boston Medical Center, Boston, MA , , Houston Methodist, Department of Anesthesiology, Interventional Pain Medicine, Cypress, TX, Department of Anesthesiology, Saint Louis University School of Medicine, Saint Louis, MO , Boston University Chobanian and Avedisian School of Medicine, Boston, MA, and Department of Anesthesiology and Perioperative Medicine, School of Medicine, Tufts University, Boston, MA
Person:: Smith, Christina, Osuagwu, Uzondu, Hsu, Annie W., Siddique, Muhammad Uzair, Adekoya, Peju, Comrie, Frederick K., Odonkor, Charles A., Abd El Sayed, Alaa, Prabhakar, Hari, Pokuri, Sreekrishna, Locklear, Jacob, Paek, Jonathan, Martins, Yuri Chaves, Palaniappan, Sarvesh, and Adler, Alexandra
Publisher:: Multidisciplinary Digital Publishing Institute (MDPI)
Rights Statement Label:: In Copyright

35. Examining Discordance in Perceptions of COVID-19 and Influenza Vaccine Safety During Pregnancy Among a Cohort of US Adults

Title Tesim:: Examining Discordance in Perceptions of COVID-19 and Influenza Vaccine Safety During Pregnancy Among a Cohort of US Adults
Creator:: Balasubramanian, Subha, Penrose, Kate, Parcesepe, Angela, Chan, Bai Xi Jasmine, Teasdale, Chloe, Nuñez Sahr, Josefina, Sanborn, Jenna, Piltch-Loeb, Rachael, Fleary, Sasha, and Robertson, McKaylee
Date of publication:: 2026
Abstract Tesim:: Background/Objectives: Vaccination is an important strategy to protect pregnant people, fetuses, and infants from severe influenza and COVID-19. However, as of April 2025 in the United States, only 14% of pregnant women had received the 2024–2025 COVID-19 vaccine, while influenza vaccine uptake among pregnant women was 38% compared to 57% in 2020. Our study assessed the perceived safety of receiving these vaccines during pregnancy within a community-based national cohort. Methods: Participants reported safety perceptions of vaccination during pregnancy and were categorized as (1) endorsing the safety of both COVID-19 and influenza vaccines, (2) endorsing the safety of only the influenza vaccine, (3) endorsing the safety of only the COVID-19 vaccine and (4) endorsing the safety of neither vaccine during pregnancy. We examined sociodemographics, behaviors, and beliefs correlated with these four categories. Log-binomial models were used to estimate the prevalence ratios of (a) endorsing the safety of only influenza vaccine versus endorsing the safety of both COVID-19 and influenza vaccines during pregnancy, and (b) endorsing safety of neither versus both vaccines during pregnancy. Results: In total, 40% of adults (N = 1725) endorsed the safety of both vaccines during pregnancy, 18% (N = 751) endorsed the safety of only the influenza vaccine, 4% (N = 183) endorsed the safety of only the COVID-19 vaccine, and 38% (N = 1621) did not endorse the safety of either vaccine during pregnancy. Participants who were non-Hispanic Black, reported inconsistent vaccination habits, and expressed low trust in government and healthcare providers were more likely to endorse the safety of only the influenza vaccine or neither vaccine compared with endorsing both vaccines. Conclusions: These findings highlight the need to build trust in the medical system, reduce access barriers, and use equitable, community-driven messaging to strengthen confidence in the safety of receiving the COVID-19 and influenza vaccines during pregnancy.
Resource type:: Article
Affiliation Label Tesim:: Gillings School of Global Public Health and Department of Maternal and Child Health
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/sgk2-x012
Edition:: Publisher
Identifier:: PMCID: PMC13030822, DOI: https://dx.doi.org/10.3390/vaccines14030274, and PMID: 41893810
ISSN:: 2076-393X
Journal Issue:: 3
Journal Title:: Vaccines
Journal Volume:: 14
Keyword:: existential psychiatry, amateur women’s football players, hypocalcemia, yellow fever, social network analysis, clinical factors, marine, LC–MS/MS, cholecystectomy, membrane, respiratory infections, heart failure, dapagliflozin, toll-like receptor, Malpighia glabra, foot deformities, reticulocyte Hb expression, earthquake, immunization, nurse retention, endurance running, NLRP3 inflammasome, Primarily gasless laparoscopy, liver fibrosis, mechanical properties, cluster analysis, bronchopulmonary dysplasia, sotagliflozin, vanadium, genome annotation, PHQ-9, oxidative stress, double laser zona drilling, counter-current flow, zero dose, CCHS, real-world evidence, 4-hydroxy-2-nonenal (HNE), TiNbSn alloy, adolescence, hypoxia, complications, reverse vaccinology, erythrocyte sedimentation rate, dual-camera system, dental visits, stress resilience, fungal genomics, precision medicine, enzymatic membrane reactor, sepsis, determinants, glutaminase-1, pediatric reference intervals, occupational health, smoking, mitochondria, apple polysaccharides, stroke, spinal alignment, cultivars, dentist-patient communication, drone detection, adolescent, bioactive glass, naringin, AI-assisted diagnosis, public health preparedness, nickel tungsten oxide, biomonitoring, hand, spinal arachnoid web, metabolome, retina, paradoxical embolism, apple pomace, assessment, sanitizer, functional foods, pediatric hypertension, predictive model, digital health, drug residues, Wnt/β-catenin signaling, antitumor activities, IDH, post-traumatic stress disorder, amino acids, development, anemia, challenges and opportunities, pharmacological mechanisms, temperature, agri-food sector, male depression, ganglioside GM3, extension, sulfurous thermal water, Equus caballus, liquid chromatography–tandem mass spectrometry (LC–MS/MS), radiomics, health, amino functionalization, sow, light intensity, computer-aided screening, adherence, genomic features, emergency care, capital income, fracture, navigation, osteopontin, rosmarinic acid, mastoid pneumatization, EjOSC, label-free identification, loquat, contrast-enhanced MRI, anti-aging, multi-epitope vaccine, positive psychiatry, untargeted metabolomics, cat, treatment outcomes, lactate, pathobiont, barium, allorecognition, psychological resilience, ACE inhibitor, Asian seabass, ultrasonography, cancer immunotherapy, MRI, CSF leak, foot biomechanics, oral hygiene, embolism, sheep breeds, canagliflozin, child mental health, circadian rhythm, no-reference image quality assessment, growth-defense trade-off, relative energy deficiency, older adults, service and delivery, LEAF-Q, diabetic cognitive impairment, D17 cells, insomnia, vaccination, agency, ankle-brachial index, GHG calculation, communication, manual therapy, inertial sensors, neonatal calf, metabolomic pathways, ADH activation, ring-enhancing brain lesions, neurodegenerative diseases, mediation, precision agriculture, superficial siderosis, milk, formal childcare, metabolism, inborn errors of metabolism (IEMs), inflammation, geogenic background, Pleurotus ostreatus, hybrid filtration–adsorption, spinal cord herniation, healthcare management, EGCG derivatives, antibacterial activity, perioperative metabolic–inflammatory modulation, Penicillium sclerotigenum, cutting guides, vaccine, acute pelvic pain, placenta, occupational diseases, emergency medical services, acute myocardial infarction, Oklahoma, emotion regulation, immune response, erythropoiesis, spinal injury, immunity, investigational drug, source attribution, co-pyrolysis, Brix refractometer, kidney, multiple myeloma, oral wound healing, people living with HIV (PLHIV), body mass index, recreational exercise, reticulocyte volume, equine internal medicine, epistemic trust, ischemia, brain metastasis, psychological support, AMP-activated protein kinase, robot-assisted surgery, diabetic foot ulcers, injecting drug users (IDUs), resistance, LPA receptors, liver cancer, rapid infectious disease diagnostics, lysophosphatidic acid (LPA), heavy oil biodegradation, pulmonary, circular economy, MMR, reactive oxygen species, interfragmentary strain, anti-inflammatory, MIC, Mongolian horse, presoaking, meat products, weaning, gut microbiota modulation, antioxidative activities, posture, lactoferrin, light scattering, irritable bowel syndrome, high-cost drug, chemometrics, mesoporous bioactive glass, method agreement, immunometabolism, Chest Pain Score (CPS), polymorph transformation, precision oncology, preterm rabbit, sex differences, balneology, mycotoxins, digestive enzymes, dun, multivariable regression, empagliflozin, quantitative proteomics, DTaP, YOLOv11, physical activity, coronary fistulas, micro-assembly, human organoids, targeted therapy, interaction, Salmonella, pig behavior recognition, transcriptional regulation, androgen receptor, triage, rice seedling, TIMI risk Score, iPSC-derived cardiomyocytes, knee injury, photochemical regeneration, multidrug-resistant bacteria, precision nursing, nephrology, EPA and ARA, vaccine hesitancy, cardio–renal axis, steatosis, apple variety, ambulatory blood pressure monitoring, poultry by-products, venetoclax, chronic kidney disease, efficacy, tuberculosis prevention, Acute Coronary Syndrome (ACS), cardiovascular disease, myopathy, computerized medical records systems, postmenopausal women, energy metabolism, RGB-HSV feature fusion, inconstant foramen, lipids, bereavement, sports medicine, sustainable water remediation, sleep disturbance, chest pain, omega-3 fatty acids, emulsified sausages, chronic disease prevention, strabismus, pyelonephritis, oral cancer, dental rehabilitation, FLT3, secondary traumatic stress, ultra-processed foods, antibiotic growth promoter alternative, bibliometric analysis, monocytes, protein–mineral mass, Bayesian analysis, diol, pandemic (COVID-19), migraines, functional properties, multimodal fusion, pandemic, triterpene metabolites, community stability, stabilization mechanism, ambulance, multi-element fingerprinting, anatomy, cancer, ventral spinal fluid collection, strontium, health policy, foot, micro-vision, reperfusion, emergency department, retrospective study, litter performance, thermostability, vitamins, HEART score, sleep satisfaction, comparison analysis, special health care needs, biodegradable plastics, Shift Work Schedule, crop improvement, developmental language disorder, thrombus, hospital workers, Acinetobacter baumannii, biomarker discovery, transgenic mouse, edible oils, Box–Behnken design, abdominal obesity, food pleasure, retinal ganglion cells, real-time object detection, nutritional modification, artificial intelligence, PHOX2B mutation, Parkinson’s disease, social–emotional functioning, retrospective studies, enteric disease, bioremediation, lipid–protein complex, amantadine, pulsatile tinnitus, MAPK and NF-κB, podocytes, fatty acid synthase, physical therapy, periadrenal varix, m6A methylation, C-reactive protein, resistance training, anxiety, hepatic stellate cells, safeguards, trauma, endothelial dysfunction, metacompetencies, systematic review, spinal cord tumors, chronic critical illness, transplantation immunology, reactive nitrogen species, intestinal barrier function, SGLT2i, nitrogen-containing platform molecules, organ-on-chip, milk withdrawal period, phacoemulsification, three-dimensional modeling, ascending thoracic aortic aneurysm, Sambucus nigra, standard urine culture, forestry waste, exercise, intravenous lidocaine, tumor microenvironment, attention mechanism, mechanism of synthesis, visceral hypersensitivity, workforce sustainability, tobacco users, preimplantation genetic testing for aneuploidy (PGT-A), circular plastic economy, taxonomic revision, Versius surgical system, HIF-1α, ophthalmic imaging, non-linear dynamics, embolic stroke, IL-6, leader–member exchange, foramen of Vesalius, oxidative balance score, levodopa, mast cell, telewellness, colostrum specific gravity, type 2 diabetes, cone-beam CT, women’s health, macrophages, inflammatory–metabolic crosstalk, N-S equations, CRISPR metabolic engineering, gastric cancer, temperament, behaviour, vaccine formulation, fibroblast senescence, ultrasound contrast agent, chlorine tolerance, VMAT-SIB, macrolide antibiotic, cyclooxygenase 2, occupational stress, autofocus, diversity, U-Net, lekethromycin, internalizing and externalizing symptoms, extracellular vesicles, pituitary fossa, myocardial fibrosis, skull base, organic acids, contrast-enhanced ultrasound, rehabilitation, glycosaminoglycans, molecular docking, propolis, mammary epithelial cells, yuba, breast cancer, pharmacokinetics, epicardial adipose tissue, hexokinase-2, laser, hyperspectral imaging, PSA, microcirculation, diagnostic clinical score, burnout, exercise participation, nanomedicine, pes cavus, expenditure, synthetic consortia, pigmentation, ethylene response factors, attachment styles, timely response, research trends, TRP, employees’ health insurance, DPP-IV inhibitor, by-products, emotional bonding, gut health, adolescent/young adult (AYA), managed entry agreement, RICD, hyaluronic acid, chronic disease, provider perspectives, gels, oil palm empty fruit bunch, mentalization, metformin, anthropometry, medically compromised patients, ageing, microwave, JAK2 V617F mutation, depressive disorder, thermal regeneration, liproxstatin-1, caring, 3D printing, primary education, biology, epigenetics, ICS, periodontal therapy, remote sensing change detection, Idesia polycarpa, non-replicating Tiantan vaccinia virus (NTV), diabetes duration, premenstrual symptoms, PICS, metabolic mechanisms, feasibility study, corn peptide, lipid-poor adrenal adenoma, biological fixation, non-pharmacological intervention, polysaccharide utilization locus, health promotion, polycythemia vera, osteological collection, octenyl succinic anhydride esterified porous starch, balneotherapy, energy storage device, visceral crisis, C/H/N/O stable isotope, generative artificial intelligence, inorganic biomaterial, diagnostic imaging, fall risk, diagnostic test accuracy, enzalutamide, skeletal muscle, flavivirus, radiological displays, physiological mechanism, cardiac regeneration, jack bean, KoGES, training, angiogenesis, gene ontology, social determinants of health, pediatric hepatitis C virus (HCV) infection, Eastern Mediterranean Region, phased haemodialysis start, Lactobacillus rhamnosus, anxiety disorders, ATCAPA, foramen of Arnold, functional seafoods, medical informatics applications, hypervascular adrenal metastasis, EV bioengineering, fluorescence, patient discharge, organic–inorganic composites, autophagy, drug delivery, paranoia, invertebrates, nitrogen sources, carriage, lifestyle factors, Canada, psychological distress, passive immunity, surgical stress response, CFD, G protein-coupled receptors (GPCRs), suicide, geometric morphometrics, miR-146b, hypertension, microscopy image classification, nanoplastic pollution, activity, aquaculture, malondialdehyde, sterol profiling, MENTOR program, anticancer activity, immunisation, fatty acids, redox signaling, child health, atherosclerosis, health insurer, boron, functional mobility, functional biomaterials, China, liquid chromatography–tandem mass spectrometry, drying kinetics, cultured meat, lumbar injury, diabetic foot ulcer, boronosteroids, adenoviral vector, movement behaviors, quality of life, synergistic effect, chronic hepatitis C, antioxidant defense, brain abscess, healthcare, year variation, probiotics, reinforcement learning, Nigrospora musae ST1, plantar pressure, soymilk, cytoglobin, myogenesis, Trichoglyphs, behavioral intentions, peri-implant disease, influenza vaccine, ecological risks, secondary traumatic stress scale, security, childhood disability, biofloc, geographical origin traceability, athletic injuries, endophyte, multi-task deep learning, mental health, smart window, Porphyridium, Bayesian predictions, lifestyle, acute myeloid leukemia, fermented longan peel, DIA quantitative proteomics, metastatic triple-negative breast cancer, caspase-1, clonal evolution, menstrual health, dementia, genome-skimming, VEGF signaling, mobility limitations, procurement, phytochemicals, chronic obstructive pulmonary disease, adoption and integration, metabolites, oral mucositis, narrative review, Environmental influences on Child Health Outcomes (ECHO), vasculogenic mimicry, amoxicillin, pediatric dentistry, carnosine, blastocyst biopsy, food security status, coronary artery, polymer semiconductor, perceived social support, photocatalytic coupling reaction, YOLO11, nurses, systemic inflammation, cold-air pre-cooling, retinal ischemia–reperfusion, personalized medicine, low energy availability, cost saving, patient centered care, observational study, MAC-T cells, bioavailability, quantitative research, upper airway obstruction, Chlorella sorokiniana, functional development, gender, sputtering, muscles, lung slice, UAV dataset, wheat milling by-product, depression, adulteration, ethology, Bider markings, apoptosis, access, phytosterols, aortic growth rate, health disparities, COVID-19 vaccine, flexion, depletion kinetics, mathematical models, extraction, metabolomics, spatial-frequency modeling, molecular point-of-care testing, kidney failure, yeast, rare species, whole wheat, ARCAPA, glycosaminoglycan lyase, Unmanned Aerial Vehicle (UAV), spontaneous intracranial hypotension, metabolic biomarkers, risk factors, general anesthesia, epidemiology, molecular pathology, hypervascular adrenal nodule, V. volvacea, health services, respiratory tract infections, palladium nanoparticles, Self-Determination Theory, adrenal washout, canine cadaver model, clinical mastitis, tendons, vaccines, ketosis, motor development, immunotherapy, positive health, acrylamide, training patterns, GOLD classification, natural polyphenols, chronotype, predictive modeling, well-being, Roseihalotalea indica, regenerative medicine, somatometric indicators, minimally invasive surgery, antioxidant, XOD inhibition, pes planus, microbial communities, insecure attachment, immunomodulatory, bovine colostrum, conspiracy beliefs, consumption motivations, life satisfaction, bovine muscle stem cells, Nile tilapia, NF-κB/MAPK signaling, vessel density, autonomic dysfunction, cardiomyocyte maturation, elevation, NF-κB, amenorrhea, anti-inflammatory activity, marine bioactive compounds, disability perspectives, oral cancer examination (OCE), mucus proteins, sleep problems, mastitis, sidedness, immunosuppression and catabolism syndrome, EjCYP716A, Paraproteinemias, PI3K/AKT, algal biotoxins, chronomedicine, oral cancer screening, COVID-19, vulnerability to climate change, swine, proteolysis targeting chimera, Poiseuille flow, nicotine, lignocellulose degradation, osteogenic mineralization, percutaneous closure, hematocrit, skin aging, electromagnetic field therapy, dentists, genotyping, calcium signaling, photobiomodulation, postbiotic, research participation, brachycephalic obstructive airway syndrome, METTL3, green technologies, co-citation analysis, postpartum period, nosocomial pathogen, alcohol consumption, atrial septal defect, socioemotional development, dry skull, TMJ ankylosis, master athletes, information and communication technology, public–private partnership, exosomes, clam shell adsorbent, emotional freedom techniques, culture conditions, freeze-drying protective, gene expression, silicone gel insole, bentonite tubular membrane, dairy goat, omega-3 polyunsaturated fatty acids, DASS-21, steroids, robotic-assisted radical prostatectomy, biochemical composition, case report, histology, dilatation of ascending aorta, treatment of textile wastewater, carbon emissions, pregnancy, metabolic characteristics, mathematical model, developmental psychopathology, magnetic resonance imaging, biological aging, outbreak detection, quercetin, ChAdOx1, children hepatitis, fungal pathogens, electrochromic devices, consumer inspiration, Oviedo Sleep Questionnaire, exercise metabolism, Panax ginseng, telemedicine, coronary thrombosis, social media marketing activities, dermatomycoses, Zanthoxylum schinifolium Sieb. et Zucc., gut microbiota, wrist, whole genome sequencing technology, gelation, xanthan gum, scale, sustainable aquaculture, job satisfaction, fermentation, myeloproliferative neoplasms, patient discharge summaries, clinical decision support, ALCAPA, liver circadian clock, trophoblast, body composition, spirulina, growth productivity, food waste valorization, antioxidant capacity, waste valorization, optimization parameters, traditional dry sausage, prostaglandin E2, intracellular signaling peptides and proteins, borates, mitochondrial dysfunction, offloading, diagnostics, metabolic reprogramming, EPR, VOSviewer, jasmonic acid (JA) signaling and JA response, association, motor variability, network analysis, children, biomarkers, celecoxib, microwave-assisted extraction, nonthermal treatments, cardiovascular risks, toe-brachial index, tuberculosis, urinary tract infection, emotional health, Lactiplantibacillus plantarum FS4722, industrial wastewater treatment, multiplex PCR, water buffalo milk, molecular phylogeny, molecular profiling, exercise-related injuries, cardiovascular risk, oxidative phosphorylation, common marmoset, mitochondrial biogenesis, antioxidation, sigmoid sinus, social support, KEGG pathway, access to health services, CT myelography, early childhood caries, wound healing, transformational leadership, adverse reactions, Boletellus, health information systems, LC-MS, non-syndromic patients, immunogenicity, rheology, work environment, BCL2, dissolving microneedle (DMN), mixed lymphocyte culture, psychotherapy, pan-cancer, DNA methylation, whole-genome sequencing, BRAF fusion, pharmaceutical policy, head and neck cancer, plant growth promoting, sustainable meat processing, risk stratification, ferroptosis, eco-friendly ceramic filters, biomass-derived material, computed tomography, percutaneous aspiration thrombectomy, chemical oxygen demand (COD) removal, emergency medicine, Darcy’s law, rubella, automation, hematopathology, diabetes subtype, pulmonary function test, disability community, Nrf2 activation, caries, machine learning, exclusively breastfed, mobility, residual attention module, non-targeted metabolomics, public health, rapid molecular diagnostics, sleep quality, sperm cryo-damage, PAR2, piglets, livestock, post-weaning diarrhea, pyroptosis, textual analysis, dental occlusion, implantation rate, gender difference, receptor pharmacology, remote patient monitoring, NRF2, mass spectrometry, incremental HD, cellulose II nanocrystals, measles, positive synergistic effect, antioxidant activity, organizational factors, diabetes mellitus, prostate cancer, attachment, gintonin, Kazakhstan, choroid, tumor angiogenesis, dyskinesia, centroid size, medical assistance in dying, colostrum composition, bioactive peptides, mitochondrial respiration, pathways, common species, cataract, enterotoxins, COG functional classification, acylcarnitines, green synthesis, qualitative research, MENIN, targeted therapies, wastewater, pediatric radiology, TCGA, female players, digital transformation, healthcare utilization, surveillance, elderly, antifungal agents, immune defense, low Young’s modulus, neurovascular dysfunction, preeclampsia, phaeochromocytoma, health-related quality of life, pathogenic yeasts, CiteSpace, fermented coffee cherry pulp, transfer learning, Nrf2/HO-1 signaling pathway, lower extremity, innovative drug, nutraceuticals, live birth rate, review, protein hydrolysate, adult horses, yam, hypochlorite, sustainability, drug development, clinical scores, dialysis treatment, Thai native chickens, hope, liver dysfunction, budget, lightweight detection, eye tracking, 1H-NMR metabolomics, fatty acid distribution, phenolic compounds, deep learning, myology, EGR1/p53/xCT pathway, VSP, patient reported outcome measures, trigger points, CT examination, diabetic cardiomyopathy, metritis, intercellular signaling, adaptive segmentation, internal migrants, castration-resistant prostate cancer, overweight, adrenal nodule, osteogenic differentiation, alloreactivity, adsorption process, barometric whole-body plethysmography, pelvic alignment, barriers, dural tear, PSA dynamics, oversight, bovine β-casein-derived peptide, canine osteosarcoma, temperate forest, group fitness, decay, non-small cell lung cancer, musculoskeletal disorders, diabetes, and transcriptomics
License Label:: Attribution 4.0 International
ORCID:: , https://orcid.org/0000-0002-7808-7439, https://orcid.org/0000-0002-4321-125X, https://orcid.org/0009-0005-1459-836X, and https://orcid.org/0000-0002-8426-6572
Other Affiliation:: Institute for Implementation Science in Population Health, Graduate School of Public Health and Health Policy, City University of New York (CUNY), New York, NY and
Person:: Balasubramanian, Subha, Penrose, Kate, Parcesepe, Angela, Chan, Bai Xi Jasmine, Teasdale, Chloe, Nuñez Sahr, Josefina, Sanborn, Jenna, Piltch-Loeb, Rachael, Fleary, Sasha, and Robertson, McKaylee
Publisher:: Multidisciplinary Digital Publishing Institute (MDPI)
Rights Statement Label:: In Copyright

36. Creation of an Engineered Oxygen-Insensitive L-Glutamate Oxidase for the Application of Electrochemical L-Glutamate Sensors

Title Tesim:: Creation of an Engineered Oxygen-Insensitive L-Glutamate Oxidase for the Application of Electrochemical L-Glutamate Sensors
Creator:: Asano, Ryutaro, Ikebukuro, Kazunori, Sode, Koji, Hatada, Mika, Takamatsu, Shouhei, and Tsugawa, Wakako
Date of publication:: 2026
Abstract Tesim:: L-glutamate (L-Glu) is the primary excitatory neurotransmitter in the mammalian central nervous system. Developing a real-time monitoring system is essential to understanding the onset and progression of related conditions. However, the absence of an L-Glu dehydrogenase that is insensitive to oxygen limits the development of oxygen-independent electrochemical enzymatic sensors. Additionally, the most commonly used L-Glu-specific oxidase requires site-specific proteolytic post-translational modifications in specific host microorganisms, which makes protein engineering difficult. To address these issues, L-Glu oxidase derived from Streptomyces mobaraensis (SmEOx), which does not require post-translational modifications, was engineered to function as a dehydrogenase. Residues crucial for the oxidative half reaction with oxygen in SmEOx were identified, and mutagenesis studies were conducted. Mutant SmEOx variants with suppressed oxidase activity and improved dye-mediated dehydrogenase activity compared to the wild-type enzyme were successfully obtained. The ratio of dehydrogenase activity to oxidase activity (Dh/Ox) increased ~2900-fold in mutant M117I and ~6700-fold in mutant M117F/K400N compared to wild-type recombinant SmEOx. The resulting virtually L-Glu dehydrogenases (vEDHs) were modified with a redox mediator and evaluated using transient open-circuit potential (OCP)-based L-Glu measurements. As a result, the vEDH (M117F/K400N mutant)-immobilized electrode enabled electrochemical L-Glu detection under ambient oxygen without the need for an external electron mediator, unlike the wild-type enzyme. The created vEDH, together with the OCP sensor developed using it, paves the way for future development of miniaturized, real-time L-Glu monitoring systems with high temporal and spatial resolution.
Resource type:: Article
Affiliation Label Tesim:: School of Medicine and UNC/NCSU Joint Department of Biomedical Engineering
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/yt8v-0136
Edition:: Publisher
Identifier:: DOI: https://dx.doi.org/10.3390/ijms27062831, PMCID: PMC13026155, and PMID: 41898688
ISSN:: 1422-0067
Journal Issue:: 6
Journal Title:: International Journal of Molecular Sciences
Journal Volume:: 27
Keyword:: lipopolysaccharide, non-contrast-cardiac computed tomography, Akt/NF-κB, early pregnancy, docosahexaenoic acid, microfluidics, Global Burden of Disease, isomer differentiation, obesity, coronary artery disease, essential oil, baclofen, multiple sclerosis, mitosis, bollworm, pain severity, neurovascular coupling, Th1/Th17 immune signaling, A2.2.2 and B2 genotypes, ecosystem service, epigenetics, phytosanitary treatment, antibiofilm, primary cilia, hospital, cardiogenic shock, cardiovascular risk assessment, IPM, years of life lost, epilepsy, gene expression phenotypes, combined central and peripheral demyelination, posttraumatic growth, tumor, neutrophil, negative-pressure wound therapy, epithelial cells, cancers obesity connex, rhythm, positive outcome expectancies for aggression, cold tolerance, Alzheimer’s disease, tucatinib, Pakistan, pathological complete response, CNS progression, mechanical circulatory support, sodium–glucose coupling, interstitial lung disease (ILD), nervous system, IL-1β refractoriness, low-FODMAP diet, luminal cell lines, ectopic bone formation and MSC differentiation, circadian blood pressure pattern, neurotoxicity, rice, Botrytis cinerea, hepatic ectopic pregnancy, T helper cells, Perk/eIF2α/ATF4/CHOP, antiparasitic activity, soil contamination, vascular dysfunction, Acheta domesticus, oxidative stress signaling, immune system, anti-fraud warning, zoledronic acid, Beckwith–Wiedemann syndrome, topical probiotics, chronic subdural hematoma, MYO1E, ciliated protozoa, diabetic retinopathy, adolescents, gangliosides, intervertebral disc, adiponectin, macular edema, drug-resistant epilepsy, sexual disorders, suicide, serotonin transporter (SERT), cardiovascular diseases, biological activities, liver injury, Mediterranean diet, precision diagnosis, CRISPR technologies, thalassemia, EWSR1::ATF1 fusion, health system planning, chitin-triggered immunity, rumen fermentation, mast cells, brain derived estrogen, qPCR, environmental monitoring, tautomerase, remineralization, mobile payment, hemidesmosomes, total phenolic contents, patient satisfaction, minimal change disease, ABC transporters, probiotics, PREVENT equations, vascular ageing, Saudi Arabia cultivar, melanoma, acute-phase response, oxidized MIF (oxMIF), RTx, synaptic plasticity, cellular necrosis, free fatty acids, panitumumab, osteotoxicity, heterotopic ossification, κ-opioid receptor (KOR), cardiometabolic risk, academic achievement, geese, malvaceae, nursery pig, podocytes, clonal hematopoiesis, CCT361814, Phyllonorycter spp., 16S rDNA, cell death, mortality, Zeugodacus tau Walker, hand injuries, Apis mellifera, atherogenic index of plasma, aortic valve, Anthonomus pomorum, randomised controlled trial, cytokines, serum protein electrophoresis, disease control, liver X receptor, bleeding risk, encorafenib, muscle nutritional quality, molecular epidemiology, dihydrolipoamide dehydrogenase, primary peritoneal carcinoma, sleep disordered breathing, transcriptome analysis, podocyte cytoskeleton, egg laying, right ventricular pacing, Ménière disease, echocardiography, vitreous biopsy, nationwide analysis, metabolomics, prognosis, toxicology, familial, miR-27b-3p, microglial activation and neuroinflammation, temporal probability, medullary thyroid carcinoma, video head impulse test, basophils, astrocytes, laminar airflow, dietary patterns, transcriptome sequencing, dexamethasone, precision, Methotrexate, group polarization, photobiological activity, federated learning, angiopoietin-like protein 2, ANGPTL, osteosarcoma, chicken embryo extract, osteochondrogenesis, BRAF, non-coding variants, ambulatory blood pressure monitoring, ‘Skeena’, personalized, eye diseases, doxorubicin, vascular structure, patient perception, LIPUS, double product, ovarian cancer, signaling pathways, molecular dynamics simulation, latent profile analysis (LPA), patient outcome assessment, HERV-H, Cydia pomonella, insulin receptor, roux-en-Y gastric bypass, premature ovarian insufficiency, hepatitis B vaccine, viral surveillance, depression, subclinical depression, vitreoretinal lymphoma, MAGL inhibition, hospital admission, ‘Sunburst’, survival outcomes, endurance exercise, redox environment, personality, tretinoin, EfficientNetV2-S, Plasmodium falciparum, transcranial Doppler, glucose tolerance, sleeve gastrectomy, PD-L1, South Greece, MAFB, behavioral patterns, Streptococcus sanguinis, Longissimus lumborum, heart rate recovery, L-glutamate oxidase, canine mammary cancer, dairy cows, acute laminitis, methicillin-resistant Staphylococcus aureus (MRSA), biomarkers, nucleus pulposus, pulmonary hypertension, siRNA therapy, surgical flaps, cystic fibrosis, glutaminase, Riptortus pedestris, internal architecture, hepatocellular carcinoma, airborne contamination, STEP, blood–brain concordance, radiation-associated, facial anomalies, SFRP, RNA interference, non-hallucinogenic psychedelics, pediatric nephrology, phytopathogenic fungi, endothelial and microvascular dysfunction, CPAP intolerance, genetic factors, lipid peroxidation, microRNA, HNSCC, multimorbidity, microorganisms, spinal alignment, ectopic pregnancy, bone microenvironment, schistosomiasis, microtubules, perioperative chemotherapy, CDKL5 deficiency disorder, radiation-induced, retrograde intrarenal surgery, right ventricle, population expansion, psychological distress, genetic differentiation, diabetes, diagnostic yield, spasticity, presynaptic region, redox state, seasonal dormancy, gene family, macrophage migration inhibitory factor (MIF) polymorphisms, enamel, post-banding ulcer, alloimmunization, ipilimumab, sirtuins, endophthalmitis prevention, quality of life, GPX4, gene expression, intravitreal injection, microglia, PTP1B, machine learning, metformin, HIFU, deafblindness, liquid biopsies, Fabry cardiomyopathy, midkine, environmental stressors, chronic unpredictable stress (CUS), SNORA12, focal therapy, ciliary neurotrophic factor, CCT gene family, fatty acid profile, steroid-resistant nephrotic syndrome, exercise capacity, group alliance, stroke, gallop race, athlete personality types, Alzheimer’s, disease susceptibility, precision nutrition, human metapneumovirus, AI-adoption signaling, Nrf2, body surface area, chronic inflammatory demyelinating polyneuropathy, clinical gait analysis, focal segmental glomerulosclerosis, probiotic supplementation, deep learning, orange, prognostic factor, tenocytes, manhood, biofilms, fat mass, quasi-direct electron transfer, acute myocardial injury, self-deception, L-glutamate, antibodies, major depressive disorder, TAVR, tumor mutational burden, cardiomegaly, Porphyromonas gingivalis, trauma-induced coagulopathy, reproduction, snoring, longevity, teriparatide, targeted therapy, brain development, serum amyloid A, Mandarin, clinical case, Th17 cells, scale, eicosapentaenoic acid, ursodeoxycholic acid, hyperglycemia, renal cell carcinoma, anhedonia, Big Five traits of student athletes, autoinflammatory diseases, EXO70E2, Populus, sporadic, monocyte, MASLD, scleroderma, colonic fistula, glomerular filtration barrier, gastric cancer, anticoagulation, JAK-STAT1/3/5 lipidomics, monocyte-to-lymphocyte ratio, audiometry, school, atorvastatin, Fabry disease, predictive utility, pyrethroids, capsaicin, extracellular vesicles, electrospray ionization, reproducibility of results, sciatica, lipid profile, lifestyle factors, cirrhosis, Coilia nasus, socio-emotional well-being, centrosome anchorage, 6-min walking test, sex differences, morphology, cardiac alterations, RANKL, esophageal varices, intestinal health, carcass composition, photodynamic therapy, nitrosative stress, right thoracotomy, subjective value, tizanidine, athlete academic performance, subjective memory complaints, precision oncology, tumor microenvironment, upfront surgery, treadmill exercise testing, AI self-efficacy, motor neuron disease, polyethylene terephthalate, tandem mass spectrometry, Parkinson’s, RNAi, PP1, musculoskeletal diseases, mitochondrial bioenergetics, peritonsillar abscess, temporal preparation, special educational needs, ECMO, testicular neoplasm, Arabidopsis thaliana, Prunus avium L., genetic and epigenetic regulation, specificity of the antibodies, shelter, redox reactions, brain endothelial cells, transcription factors, genetics, dye-mediated dehydrogenase activity, wound healing, single-cell transcriptomics, TGF-β signaling, femoral arterial cannulation, sex, leptin, heat shock response, genes, gut microbiome, bone mineral density, inflammatory and nested testicular sex cord tumor, virulence factors, immunotherapy, relationship, HIF-2α, skin fibrosis, autosomal recessive inheritance, adolescent, Gram-positive bacteria, double-coil rPMS configuration, neoadjuvant treatment, BKPyV, metabolites, atherosclerotic cardiovascular disease, schizophrenia, APOE, disc degeneration, SKOV3 cells, mass rearing, nitrergic signaling, chest X-ray, antioxidant therapy, nanoparticles, severe trauma, renal dialysis, phylogeography, surgery, osteoporotic vertebral fracture, miscarriage, Syncytin, Interleukin-17A, neuroinflammation, bone-on-a-chip, intracerebral hemorrhage, immune evasion, aged, metabolism and immunity, vascular function, meningioma, Mexican Americans, antimicrobial activity, miracidia, oligofructose, stanines, chloropyridine derivative, experimental study, low-grade serous carcinoma, wood ash, FLOT, tumor regression grade, inhibitors, group psychology, hemolymph physiological indices, eye tracking, pseudotumoral mass, brain-derived neurotrophic factor—BDNF, transient open circuit potential, amino acid, mineral content, PTEN, lipid metabolism regulation, in vitro wound healing, gene regulation, biotic stress, CD109, brain health, ω-3 polyunsaturated fatty acids, chronic kidney disease, Bidah pomegranate, SEL, synaptic maturation and neuroprotection, norbinaltorphimine (nBNI), Madin–Darby canine kidney cells, neuro-inflammation, organizational attractiveness, annulus fibrosus, PIK3CA, social categorization, trabecular bone score and mediterranean diet, ultrastructure, HPV, parasite-host interaction, steroid-resistant nephrotic syndrome (SRNS), SERT-KO mice, sleep quality, estrogen receptor, anti-MDA5 antibody, CATT repeats, survival rate, nano-hydroxyapatite, solid-supported membrane electrophysiology, SIRT1/Nrf2/HO-1, therapeutic target, early rebleeding, primary prevention, natural enemies, amyotrophic lateral sclerosis, chronotype, metagenomic sequencing, self-efficacy, PAMORA, human endogenous retroviruses, drug-induced sleep endoscopy, adipogenesis, childhood trauma, ischemia and reperfusion, perennial cropping systems, Turner syndrome, intensive care unit, multi-omics, serum alternative, HSF1, ARHGDIB, neuroimmunology, endocannabinoid, mitral stenosis, gastroesophageal junction, tone preference, metabolic syndrome, radiology, nephrotic syndrome, biodiversity, sustainable medicinal plants, postsynaptic region, dermatomyositis, FMT, needle aspiration, irritable bowel syndrome, adolescent idiopathic scoliosis, retinal vein occlusion, syndromic hearing loss, tyrosine kinase inhibitor, AML, Tempol, self-esteem, fetal bovine serum, Rana dybowskii, pathology, firmness, B cells, minimally invasive cardiac, endocannabinoid system, prognostic signature, neurotrophins, genetic diagnosis, retained surgical sponge, electron microscopy, hematoma volume, boric acid, liver cancer, security behavior, autotaxin, colorectal cancer, LysM effectors, cadmium, addiction rehabilitation, putative neuropeptide, intestinal morphology, spinopelvic balance, rheopheresis, integrated pest management, ferulic acid, pitch preference, flavonoid, gene regulatory networks, social media, muscle, blood groups, retinal cells, central aortic cannulation, antibacterial activity, multimodal imaging, endoscopic variceal ligation, arachidonic acid, lung inflammation, hydride transfer chain, cigarette, genotypic identification, NRF2, untargeted analysis, circadian rhythms, laboratory maintenance, somatic mutation, bioactives, gossypiboma, romosozumab, genotypes, copper, developmental phases, reward motivation adaptation, short-chain fatty acids, anti-Ro52, CDK, inflammation, schoolchildren, aging, bone organoids, substrate binding cleft, privacy, surgical safety, imprinting disorders, cancer stem cells (CSCs), metacarpophalangeal joint, epidermal barrier dysfunction, HCC, gene therapy, biliopancreatic diversion, abiotic stress, coated betaine, G6PD, IL-24, schoolboys, active breaks, opioid-induced constipation, secretory cell, neurotransmitter, neurorehabilitation, opinion leaders, neoadjuvant, length of stay, celiac disease, prediabetes, type 2 diabetes mellitus, periodontitis, secondary, Phytoseiidae, anti-CD20 therapy, pain control, internet, APP/Aβ, metabolic dysfunction-associated steatotic liver disease, fungal communities, molecular mechanisms, MEK, prospective study, phytotherapy, impulsivity, Golgi apparatus, miR-16-5p, immune homeostasis, mitral anulus calcification, cyclin, neuromuscular junction, perceived innovation ability, gut-brain axis, stigma, demineralization, atrial fibrillation, hydrolyzed collagen, eQTL, antioxidant phytochemicals, Local Binary Pattern (LBP), heart failure, Vision 2030, mast cell stabilizers, disease-modifying therapies, bone morphogenetic protein (BMP) signaling, systematic review, Adoxophyes orana, cerebral circulation, molecular docking, temperature, venom apparatus, intestinal microbiota, skin microbiome, dihydroquercetin, muscle injury, tabernanthalog (TBG), volatile fatty acids, cochlear implant, TRIM28, TNBC, ferroptosis, liver, drainage, mating success, cerebral vasoreactivity, hyperuricemia, hearing aids, primary hypertension, inflammatory bowel disease, blood types, Schistosoma mansoni, practice patterns, reactive nitrogen species, apoptosis, SIRI, lysosome, m6A modification, HER2-positive breast cancer, ML210, calcineurin, trauma management, cyclophosphamide endoplasmic reticulum stress, preschool children, colour, emotions, Lipid droplets, neuron, antitumor activity, abdominal abscess, oxidative stress, cardiovascular disease, hibernation, silk moth, hemorheology, synthesis, opioid, metastasis, acute manganese stress, stem cells (SCs), phytochemicals, moral cost, methylene blue, fragmentation pathways, physical activity, EMT, network cluster behavior, portal hypertension, pertuzumab, CTLA-4 blockade, renal transplantation, intrinsic apoptosis, adjuvant chemotherapy, crustacean aquaculture, G protein–coupled receptor, tumor aggressiveness, healthcare utilization, kidney transplantation, biological control, pan-cancer, demyelination, breast cancer, total flavonoid contents, osteoarthritis, resveratrol, Bauhinia thailandica, national inpatient sample, SETDB1, radical gastrectomy, adaptability to pressure overloading, aquatic disease control, fetlock, mouse model, immunisation, regional anesthesia, Squeeze-and-Excitation, Tanzania, electrogenic transport, whole-exome sequencing, sickle cell anemia, age, arterial cannulation, cryoablation, grade point average (GPA), virtual gene panels, venous thromboembolism, ecological assessment, Duane retraction syndrome, nitric oxide, HERV-K, Warburg effect, repetitive peripheral magnetic stimulation, ROS production, mitochondria, sickle cell disease, long COVID, mesenchymal stem cells, asparaginyl endopeptidase, electrospun nanofibers, meta-analysis, PETase, Vibrio parahaemolyticus sodium–galactose transporter (vSGLT), chromatographical analysis, open healthcare data, medicinal chemistry, terminal UPR, immune disorders, peripheral blood immune cells, electrochemistry, hemorrhage, honey bees, body image, knee joint, predictors, chronic disease, cardiopulmonary bypass, sexuality, vestibular system, TMVR, LVAD, online disinhibition, interleukin-2 (IL-2), chevon, non-dipping phenotype, CD177, kavalactones, angiogenesis, OPA1, therapies, duck, SOX2, enzyme replacement therapy, NT-proBNP, blood–brain barrier, autism, rehabilitation, flavor compounds, imputation model, lymphopenia, survival analysis, NF-κB, cannabinoids, cancer stem-like cells, body composition, substance use disorder, laxatives, parent training, behavioural bioassay, years lived with disability, candidate gene discovery, gene silencing, PKR, 3D-DXA, functional autoantibody, fat-free mass, pathological response, NXP800, parasagittal groove, fan-beam CT, cross-cultural sport psychology, case report, primary vitreoretinal lymphoma, exercise physiology, biventricular volume, bone adaptation, Hounsfield Unit, DMT1, extracts, ADMET, bariatric surgery, psychoplastogens, race, computed tomography, recurrence, molecular dynamics, left ventricular ejection fraction, hippocampus, children, natural killer (NK) cells, Wuzhishan pig, genome editing, key determinants, EGFR, ketotifen, HSF1 pathway inhibitor, ER stress, fruit fly, olfactory receptors, v-ATPase, aneuploidy, Saudi Arabia, nanodiamonds, protein–protein interaction, severe malaria, dominant optic atrophy, neuropsychiatric disorders, neoadjuvant therapy, brace treatment, rural health systems, autoantibody, prostate cancer, immune checkpoint inhibitor, IL-17, albumin-to-globulin ratio, computerized dynamic posturography, speech perception, scratch assay, ZnO nanoparticles, phlebotomy, metabolism, microbiota, problematic smartphone use, tau, protective factors, testicular sex cord–stromal tumor, aortic regurgitation, PI3K, anthocyanins, gastrointestinal health, gut microbiota, molecular pathology, standard human index, inpatient outcomes, global longitudinal strain, incision, eccentric contraction, subarachnoid hemorrhage, 7-ketocholesterol, functional dysbiosis, methotrexate, total tannin contents, growth stages, aggression, paraplegia, ureteral access sheath, pressure overloading, third metacarpal bone, chemoresistance, chronic pain, carotid arteries disease—CAD, pelvic injury, predatory mites, laminar tissue, colorectal neoplasms, thyroid cancer, AOM/DSS, metabolic disorders, photosensitizer, diabetic foot ulcers, antibacterial, predatory bug, zinc oxide nanoparticles, surgical protocol, Sonic Hedgehog, diabetic retinopathy staging, right heart failure, metabolic liver cancer, parental active mediation, rectal peritoneum, seborrheic dermatitis, Impella, neurodevelopmental disorders, phosphatases, ketamine, hearing loss, liver steatosis, panel sequencing, obstructive sleep apnea, MAPK, rapidly progressive ILD, equine, HER2, G 111nt duplication, triple-negative breast cancer, OsCBL-OsCIPK module, traumatic heterotopic ossification, biomarker, anthropometry, blood transfusion, effort–reward imbalance, circulating analytes, cardioprotection, dissociation, tonsillitis, EIF2AK2, growth performance, hypertension, memory confusion paradigm, e-cigarette, prebiotics, auditory neuropathy spectrum disorder, genetic classification, Western diet, and calreticulin (CALR) mutation
License Label:: Attribution 4.0 International
ORCID:: https://orcid.org/0000-0001-6795-8377, https://orcid.org/0000-0003-2838-0562, https://orcid.org/0000-0002-9833-2091, https://orcid.org/0009-0005-8098-9561, , and https://orcid.org/0000-0001-6299-5267
Other Affiliation:: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan and
Person:: Asano, Ryutaro, Ikebukuro, Kazunori, Sode, Koji, Hatada, Mika, Takamatsu, Shouhei, and Tsugawa, Wakako
Publisher:: Multidisciplinary Digital Publishing Institute (MDPI)
Rights Statement Label:: In Copyright

37. Divergent Evolution of Tuberculosis Lesions During Treatment: A Longitudinal CT-Based Analysis of Progression and Regression Patterns

Title Tesim:: Divergent Evolution of Tuberculosis Lesions During Treatment: A Longitudinal CT-Based Analysis of Progression and Regression Patterns
Creator:: Ma, Shiran, Xie, Yingda L., Lv, Pingxin, Jiang, Jiaxin, Wang, Wei, Qin, Liyi, Li, Weimin, Takiff, Howard E., Liu, Xiaoming, and Liu, Qingyun
Date of publication:: 2026
Abstract Tesim:: Objectives: Lesion-level dynamics may reveal pulmonary tuberculosis (PTB) heterogeneity and help identify factors associated with treatment outcomes. Methods: A total of 288 serial Computed Tomography (CT) scans from 125 PTB patients were obtained from the National Institute of Allergy and Infectious Diseases (NIAID) TB Portals database (2008–2023). Lesions were segmented and annotated to obtain volume and imaging features, and a conservative longitudinal volume quantification method was used to characterize dynamic volume patterns. The proportion of lesions with different patterns was analyzed at the patient level to assess trajectory diversity. Firth’s penalized logistic regression was used to identify factors associated with treatment outcomes. Results: Among 435 lesions in 125 patients, five patterns emerged: Stable, Decrease, Increase, Mix-I-D (increase then decrease), and Mix-D-I (decrease then increase). Multiple patterns coexisted in 66.7% of treatment success patients and all treatment failure patients. Mix-D-I lesions were identified more frequently in treatment failure patients (25.0% vs. 1.4%, p = 0.027), and in multivariable analysis, the presence of Mix-D-I lesions was statistically associated with treatment failure (p = 0.024). Conclusions: PTB lesions showed high trajectory heterogeneity. The presence of Mix-D-I lesions may point to an unfavorable treatment course, suggesting lesion dynamics could serve as a potential indicator for poor outcomes. By quantifying lesion-level trajectories on serial CT scans, we extend PET/CT-based evidence and support the value of routine monitoring in clinical management of tuberculosis.
Resource type:: Article
Affiliation Label Tesim:: School of Medicine and Department of Genetics
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/g96j-rw96
Edition:: Publisher
Identifier:: DOI: https://dx.doi.org/10.3390/diagnostics16060892, PMID: 41897625, and PMCID: PMC13025260
ISSN:: 2075-4418
Journal Issue:: 6
Journal Title:: Diagnostics
Journal Volume:: 16
Keyword:: lactate dehydrogenase, nutrigenetics, chemerin, cardio-oncology, leftovers, cervical intervertebral disc degeneration, gastrointestinal malignant tumors, ensitrelvir, combination index, winter swimming, Bayesian projections, irritable bowel syndrome with diarrhea, CFD modeling, ice hockey, hepatokine, α-glucosidase inhibition, diet therapy, police officers, choledochojejunostomy, exercise, mechanobiology, apparent nutrient digestibility, obsessive–compulsive symptoms, radiomitigator and radioprotector, numerical simulation, feed intake regulation, Tc-99m-PSMA SPECT/CT, scale-up, mental health, TB care cascade, role ambiguity, fan practices, PDE1, C-peptide, kidney development, network pharmacology, type 2 diabetes mellitus (T2DM), Ca2+-ATPase, L-carnitine, mixed cultures, fatty acid oxidation, Achilles tendon, dental anesthesia, antimicrobial resistance, fibrosis, high-fat diet (HFD), outcomes, computed tomography, diagnostic yield, child and adolescent mental health, SMFR, marine fuels, sequential fermentation, Degeneration Paradox, cyclic tensile strain, nuclear pore complex, molnupiravir, short-chain fatty acids, honey, quality of life, workforce, chronic insomnia disorder, pullorum disease, superficial back line, sustainable healthcare, blood profile, postmortem aging, neurorehabilitation, personalized medicine, nutritional profile, click, risk factors, PAX proteins, crotoxin, Helicobacter pylori, CE-Chirp, yellow-feathered broilers, 3CL pro, climate change mitigation, precision feeding, citrus, intraoral injection, heart rate, total flavonoids of Sea buckthorn pericarp pomace (TFSP), satisfaction, hypoglycemia, children, HPLC-DAD, microencapsulation, packaging migration, hypoxemia, ADP-ribosylation, tuberculosis, ski-slope hearing loss, dog, quercetin, barrier dysfunction, age-period-cohort analysis, buprenorphine/naloxone, evolutionary conservation, kinase–substrate phosphomotifs, emergency treatment, vestibular rehabilitation, organ indices, CRISPR Cas, blood biochemistry, desiccating stress, corneal blood staining, health education, energy system contribution, Hu sheep, Bayesian age-period-cohort, bio-CO2, prognosis, creative thinking, complications, viscoelasticity, unmet healthcare needs, mycobiota, opioids, contrast-enhanced ultrasound, behavioral health, hematocornea, trauma-related sleep disturbances, necrosis, cyberbullying behaviors, shotgun sequencing, transmission electron microscopy, biochemical recurrence, apple extract, food safety knowledge, lactoferrin, HIV, age, lymphadenopathy, cyberbullying, orthotic treatment, soil plant transfer, adipokine, hours of digital media use, ice-cold strategy, cone beam computed tomography, keeping hot food hot, Penicillium nalgiovense, bioactive peptides, adenosine kinase, photoactivated adenylate cyclase, molecular docking, eFAST, safe food handling, ADPKD, onion peels, linguistic landscapes, LC-MS/MS, PI3K/AKT signaling pathway, therapeutic adherence, feline cardiology, antagonism, technology-based parenting, antibacterial activity, systemic inflammation, cone-beam computed tomography, body mass index, muscle fibres, electronical health records, cystometry, pleomorphic adenoma, reactive oxygen species (ROS), chronic venous disease, corn cleaning, organizational commitment, adolescent girls, endoscopic retrograde cholangiopancreatography, cAMP, Muscovy duck (Cairina moschata), risk stratification, hyperlipidemia, emergency medical care, pancreatitis, structural equation modeling, sustainable development, management, global navigation satellite system, developmental gradient, biliary anastomosis, molecular biomarkers, patient experiences, keratinocytes, internal load, mortality, weight reduction programs, school health programs, Saudi Arabia, phosphoproteomics, zoonotic disease, exposure therapy, genomic analysis, cardiotoxicity, MAPK, subaortic membrane, program quality efficiency, antioxidant, absorption coefficient, thymol, cerebrovascular reactivity, Bacillus cereus, antioxidant therapy, bioaccessibility, degree of enactability, vector, rabbits, green plum cultivars, organizational citizenship behavior, meat color, wine, environmental management, SHGS, vitamin K, lactoferrin peptides, lactic acid bacteria fermentation, oral surgical procedure, TGF-β, relational aggression, pomegranate waste extract, injury-initiation attribution, feline hypertrophic cardiomyopathy, opioid dependence, struvite, trauma-induced insomnia, advanced diagnostics, PfATP6, estrogen receptor, gravitaxis, pediatric thoracic trauma, ubiquitously expressed genes, attention deficit hyperactivity disorder (ADHD), dyspepsia, lactate, nesprins, live weight, caviomorpha, healthy lifestyle behaviours, methanol, epidemiology, comorbidities, gait, glymphatic system, bisphenol S, pediatric, ellagic acid, travelers, clinical decision-making, EW-7197, Francisella tularensis, iCEB, morphine, FAST, hypothalamus, tyrosine kinase inhibitors, distal fragment length, pig, Simmental, winter bathing, lamins, clinical staging, adenocarcinoma, diabetic ketoacidosis, clinical studies, next-generation antimicrobials, botulinum toxin, BERT, interventricular septum hypertrophy, progranulin, kidney–gut axis, anomalous right coronary artery from the pulmonary artery (ARCAPA), rehabilitation, PAX3, LC-MS, cecina, cellulose, EUV microscopy, apprenticeship, locking plate fixation, oxidative status, depression, autoimmune disease, perceval, withdrawal, absenteeism, cardiovascular disease, PFAS, emotion elicitation, anchor to the present moment, metabolic syndrome, aortic elasticity, natural language processing, calcium homeostasis, reflexive thematic analysis, persistent COVID-19, wrinkles, tomato polyphenols, response surface analysis, TEM, CTRP3, phytotoxins, 3D-MERGE, chitosan, osteogenic activity, upper lip dynamics, biliary tract ections, metabolomic profiling, evidence-based medicine, microbiota–gut–brain axis, sodium channels, computed coronary tomography angiography (CCTA), Cre-loxP, CEUS, Dammam, phenolic stability, CAKUT, LVOT, Lespedeza juncea tea, adolescent and young adult, gastritis, Jeddah, plant extracts, attention-deficit/hyperactivity disorder, immunological biomarkers, biomarker, HSP70, LUTS, medication, polymorphism, mood disorder risk, Thyroid, swelling, toy-breed dogs, bovine, children and adolescents, Moyamoya Angiopathy, gender norms, meat chemistry, periodontal disease, COVID-19, perinatal outcome, error reporting, bacteriostatic mechanism, untargeted metabolomics, innate immunity, squamous cell carcinoma, health behavior, nanoplastic-enhanced arsenic toxicity, ERBB signaling pathway, Penicillium, IFITM mutants and chimeras, antibiotics, plant–virus interactions, Slovenia, Bordetella pertussis, salivary gland tumors, law enforcement, gut microbiota, hypermobility spectrum disorders, rare congenital coronary artery anomaly, genetic testing, Doppler ultrasound, neutrophil extracellular traps, CO2 capture, botulinum toxin type A, anti-nutrients, epigenetic alterations, macroporous resin, cardiovascular risk, cognition, echocardiography, CFLD, traceability, endocrine-disrupting chemicals, mitochondrial metabolism, prompt engineering, sensory analysis, animal model, immunocompromised, incidence reporting, sulforaphane, conservation of resources theory, motor proficiency, shear strength, lindoldhamine, heat stress, trigeminal neuralgia, principal component analysis, enzymatic hydrolysis, malignant transformation, lactic acid bacteria, cerebral palsy, cell wall material, self-esteem, peptide drugs, case report, HSE Management Standards, lymph node venous network, occupational health, digital technologies, microbiological quality, Holstein, health policy, verbal aggression, range of motion, target cell protection, bisphenol F, ethics, hemodynamic instability, immunity, cosmetics, karyotype, Latina adults, cultivar regions, imaging, order effects, deep anterior lamellar keratoplasty, creativity awareness, bioinformatics, corneal transplant, tissues, imaging pathway, mice, cecal microbiota, phenolic compounds, membrane gas absorption, risk perception, epigenetic factor, body composition, urban trauma, muscle strength, cytokines, forces, GBD, neuroimmunity, mouse models, conservation, zebrafish larvae, obesity, colostrum, Cylindrotheca closterium, fecal metabolome, oral microbiome, production performance, VO2max, anthracyclines, Euglena gracilis, aggressive behavior, resource-limited settings, febrile seizure, anatomic variation, attribution theory, chimeric antigen receptor (CAR) immunotherapy, reconstruction–omission, job burnout, thawing, cystic fibrosis, high-frequency hearing loss, diagnostic accuracy, programmatic surveillance, aquatic toxicity, valorisation, survival, emotional exhaustion, metastasis, calcium oxalate, digestibility, upper limb, rat, air pollution, ischemic stroke, adenosine, recovery, Ziziphus jujuba Mill., fracture stabilization, semen quality, healthcare workforce, duodenogastric reflux, goslings, dry-cured meat, bladder disease, tattoo, substance use disorder, tularemia, accessibility, magnoflorine, legal decision-making, DNA damage, cardiology, carcass traits, infective endocarditis, blue nature, focus group, broiler, sensory evaluation, CRISPR/Cas12a, Staphylococcus aureus ATCC 25923 (S. aureus ATCC 25923), meta-analysis training, intramuscular fat, arsenic accumulation, aortic valve replacement, type 1 diabetes, fluorescence-based bioassay, drug delivery, blue space, pre-aortic dissection and post-aortic dissection type A, microbial fermentation, nephron progenitor, pressure gradient, gut health, volatile organic compounds, pediatric population, trunk muscles, black soldier fly larvae, diagnosis, longitudinal, action memory, animal models, weaned piglets, knee extensors, transcription factor, recombinase polymerase amplification, ZTE, finishing, subaortic stenosis, protein-DNA interactions, ocular surface inflammation, effectiveness, schizophrenia, DALK, nanoplastics, polyphenols, koala, beef cattle, non-immersive VR, [15O]water PET, arterial oxygen saturation, skeletal muscle, trace metal toxicity, Belgian-crossbred, enactment effect, neuroinflammation, creativity enhancement, Autosomal Dominant Polycystic Kidney Disease, fictional linguistic landscapes, theaflavins, diabetes complications, dietary supplement, blood pressure determination, myocarditis, metabolic regulatory pathways, iron metabolism, microbiota, broiler chickens, antioxidant activity, Nrf2, cardiovascular risk factor, chamomile hydrolate, marine bioresources, grain quality, workplace stress, integrated TB screening, role clarity, collagen, inversion-dominant phenotype, epileptogenesis, state/trait anxiety, autism spectrum disorder, motor information reactivation, qualitative, university hospitals, climate resilience, F-actin, protective antibodies, retrospective cohort study, bioavailability, substrate, radiation fibrosis, miRNA, food proteins, neuroprotection, artemisinin, epilepsy, sleep, sphenoid sinus, insect meal, Phascolarctos cinereus, recurrence, glycemic control (HbA1c), repeated high-intensity effort, symptom severity, glaucoma, dental bonding, psoriasis, post-ERCP pancreatitis, heart valve surgery, health systems performance, muscle, screen-based virtual reality, RT-DETR, sleep health, microbial imbalances, protein–protein interaction, angiogenesis, equinus, tendinopathy, endomyocardial biopsy, cabin crew, gestational diabetes, thoracic trauma, infrared camera, phosphorylation, endothelial dysfunction, anti-tumor treatment, Gi protein, cyberbullying coping strategies, chlorhexidine, gut microbiome, sarcoidosis, characteristic volatile compounds, risk predictors, genetic counseling, daily weight gain, job crafting, heat shock proteins, lip abnormalities, authenticity, sensory processing, kitesurfing, essential oils, targeted treatment, soybean meal replacement, ROC analysis, genotype, cell tomography, barriers, nasopharyngeal carcinoma, melanocyte, personalized nutrition, env-glycoproteins, dry eye disease, perceived organizational support, organic acid, early-life adversity, carcinoma ex pleomorphic adenoma, suicidal ideation, tourists, biodiversity, breath-hold functional MRI, differential metabolites, peer recovery specialist, sediment, meta-analysis, glutamate dehydrogenase, genetic predisposition to disease, inflammatory cardiomyopathy, live performance, 3D-MENSA, TEAC, regression, proxies, immune-epidermal crosstalk, neural crest, se-enriched eggs, hormones, antioxidant peptides, gingival display, frailness, hospitalisation, sacroiliitis, primary teeth, mechanotransduction, sick leave, rumen microbiome, One Health, SERCA, monoclonal antibodies, ulcerative colitis, pulse oximetry, spatial heterogeneity, mucin alteration, Y chromosome microdeletion, postural orthostatic tachycardia syndrome, PI3K/Akt, vermilion inversion, fibrosis MASLD, bipolar disorder, cream, nucleus pulposus cells, abscess, microRNA, metabolic profiles, research capacity building, the ethyl acetate extract from the root of Schisandra propinqua (Wall.) Baill. var. sinensis Oliv (Xiao Xue Teng), endocrine-disrupting compounds, Phaseolus vulgaris, ORAC, suicide attempts, bias mitigation, personalized treatment, redox homeostasis, pediatrics, cell lines, personal appearance, microplastics, survival analysis, psychosocial work environment, boric acid, oxidized albumin, urolithiasis, late-onset hypogonadism, infertility, subvalvular aortic stenosis, cerebral ischemia, athletic training, soil physicochemical properties, transcriptomic profiling, late pregnancy, back pain, RNA, orbicularis oris muscle, creativity, apoptosis, attachment, structural binding characterization, healthy aging, transnational youth, economic feed conversion ratio, cyberbullying roles, health institutions, Med-diet, gerontology, ‘Hujing Milu’ peach, kinase, dengue virus, remdesivir, intrinsically disordered protein, nutritional education, physicians’ attitudes, insomnia, lesion heterogeneity, heat treatment of food, gender disparities, cancer, vaccines, low-cost biomarkers, individualized ALPS pipeline, environmental status, equine infectious anemia virus, zygomatic implants, signaling pathway, blue crabs, conditional knockout, dental implant, randomized controlled trials, hypoxia-inducible factor-1α (HIF-1α), feed efficiency, arm, mental well-being, soilless cultivation system, body-checking, lipid metabolism, Framingham risk score, viral fusion, exosomes, mental peace, aortic valve stenosis, ionizing radiation, uveitis, population aging, immunomodulation, agricultural soil, metabolomics, meat quality, chest radiography, in-hospital mortality, prostate cancer, cognitive dysfunction, performance, disgust sensitivity, gender differences, gut–brain axis, carcass, early diagnosis, grape pomace, matrix effect, urgent health care, DTI-ALPS, atrial fibrillation, health personnel, controlled-environment chamber, mediation, Mediterranean recipes, low-protein diet, neurocritical care, HPV, infection, evidence-based veterinary medicine, oral squamous cell carcinoma, HS-SPME-GC-O-MS, health promotion, mechanotransdcution, spasticity, functional dairy products, soft X-ray microscopy, N,N-methyldomesticinium, cardiovascular health, bushfire, educational training, Keemun black tea, p16, neurotoxicity, pediatric audiology, gas cleaning, Thalassiosira weissflogii, nephrogenesis, erectile dysfunction, mechanosensing, temporal bone, physico-chemical traits, waste valorization, HSOPSC, AHRQ, HIV negative imprinting, cyclic AMP, legume valorization, pain, dual left anterior descending artery (dual LAD), testosterone, auditory brainstem response, Lynx pardinus, treatment setting, abiotic stress pre-exposure, Heart–Brain Team, viral pathogenesis, functional films, inflammation, horse meat, time efficient, transcriptome sequencing, food safety culture, dementia, retrospective study, dysautonomia, vascular compression syndrome, Fusobacterium nucleatum, anticoagulation, wall shear stress (WSS), dizziness, large language models, abusive supervision, cholecystitis, autoantibodies, refractory COVID-19, eating disorders, machine learning, atherosclerosis, diagnostic imaging, youth athletes, gene expression, menaquinone-4, avian leukemia, hypervigilance, acute phase proteins, early pregnancy, paraprofessional, parental assessment, reproductive health, pertussis toxin, orthobiologics, temporal trends, force output, lifestyle factors, Oula sheep, subcellular localization, nurses, malignancy, glioblastoma, attitudes and practices, body image, MRI, volatile compound, genome stability, cognitive biases, diagnostic sequencing, performance-promotion attribution, off-the-track Thoroughbred, vinpocetine, VRK3, molecular toxicity, burn, neighborhood stress, chest trauma, hypoxia, tumor microenvironment, inorganic arsenic, B and T-cell epitopes, cell stiffness, GPT, host-directed therapy, growth performance, environmental exposure, balance, peripheral vestibular hypofunction, behavioral toxicity, number needed to screen, zoonosis, safety assessment, sexual function, crayfish, registry, esterified catechins, career plateauing, hypertrophy, horse, small mammal, selection signatures, phototaxis, tissue regeneration, bacteriophages, heart failure, tomography, cholangiocarcinoma, nutrition, corneal graft, precision livestock farming, disordered eating patterns, reference interval, patient safety culture (PSC), softness/stiffness, pattern recognition receptors, breed, nocturnal arousal, passive transfer, sensorimotor integration, nocturnal penile tumescence, organizational support, CBX4, acute heart failure, ATR-FTIR, infectious diseases, artificial intelligence, retrospective cohort, risk assessment, cyberbullying definition, tomato cellulose-based bioplastics, sublethal biomarkers, glucagon-like peptide 1 receptor agonists, enzyme–microbe co-fermented Ganoderma lucidum spent substrate, glucose fluctuations, nile red lipid assay, giant panda, acute hepatitis E, endoperoxides, motor information, punicalagin, socioeconomic status, vactosertib, physical health, food safety, environmental contaminants, untargeted analysis, self-determination theory, plant protein ingredients, soft tissue tumor, co-infection, cottonseed protein, veterinary postgraduate education, poultry nutrition, anxiety disorders, seasonal variation, cattle well-being, melanoma, deep learning, NF-κB, food preservation, intestinal morphometry, human exposure assessment, fecal microbiota, tropical forages, COVID-19-related anxiety, contamination fear, void spot assay, visual detection, aortic stiffness, co-parenting, Rendena, gestational weight gain, goblet cell remodeling, Chinese yam, Mendelian randomization, hydroxyapatite, disinfectants, soybean, physical aggression, Qinghai–Tibet Plateau, Pseudomonas fluorescens, antioxidant capacity, dental leakage, pediatric imaging, internal states, virulence, wildfire, implant failure, stroke, early diabetes onset, influenza vaccines, ferroptosis, socioeconomic determinants, zygomatic complications, one-tube assay, myasthenia gravis (MG), adenosine receptor, rabbit, fining, aerobic capacity, symptoms, microsporidia, session rating of perceived exertion, sex, neonatal lamb, cardiac imaging techniques, mesenchymal stem cells, speckle tracking echocardiography, radiotherapy, redox-related enzyme response, task-oriented circuit training, molecular mechanism, behavior detection networks, intestinal flora, multilingualism, sensor technologies, intrahepatic cholangiocarcinoma, Mediterranean diet, oxidative stress, and polyphasic identification
License Label:: Attribution 4.0 International
ORCID:: and https://orcid.org/0000-0002-0480-0860
Other Affiliation:: Beijing Chest Hospital, Capital Medical University, Beijing, China, Department of Medicine, Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ, USA, Department of Radiology, Being Geriatric Hospital, Beijing, China, Department of Biostatistics, New York University, New York, NY, USA, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela, and
Person:: Ma, Shiran, Xie, Yingda L., Lv, Pingxin, Jiang, Jiaxin, Wang, Wei, Qin, Liyi, Li, Weimin, Takiff, Howard E., Liu, Xiaoming, and Liu, Qingyun
Publisher:: Multidisciplinary Digital Publishing Institute (MDPI)
Rights Statement Label:: In Copyright

38. Repeatability of Semi-Quantitative and Volumetric Features from Artificial-Intelligence-Guided Lesion Segmentation on 18F-DCFPyL PSMA-PET/CT Images: Results from a Test-Retest Cohort

Title Tesim:: Repeatability of Semi-Quantitative and Volumetric Features from Artificial-Intelligence-Guided Lesion Segmentation on 18F-DCFPyL PSMA-PET/CT Images: Results from a Test-Retest Cohort
Creator:: Wisniewski, Nicholas, Gorin, Michael A., Whang, Young E., Islam, Md Zobaer, Werner, Rudolf A., Pomper, Martin G., Bundschuh, Ralph A., Pienta, Kenneth J., Weisman, Amy, Perk, Timothy G., Markowski, Mark C., Rowe, Steven P., and Milowsky, Matthew I.
Date of publication:: March 11, 2026
Abstract Tesim:: Objectives: This study evaluated the test–retest repeatability of semi-quantitative and volumetric features derived from artificial intelligence (AI)-assisted lesion segmentation on 18F-DCFPyL Prostate Specific Membrane Antigen (PSMA)-PET/CT imaging of patients with prostate cancer (PCa). Specifically, we assessed the reliability of maximum, minimum and total standardized uptake values (SUVmax, SUVmean, SUVtotal) and lesion volume measurements across varying lesion sizes and explored the implications of variability for clinical decision-making. Methods: We analyzed 18F-DCFPyL PSMA-PET/CT images from 22 patients with metastatic PCa. Lesion segmentation was performed using the AI-guided TRAQinform IQ technology, followed by a manual review to eliminate potential false-positive sites of uptake. Lesion-level test–retest repeatability was evaluated using 95% limits of agreement (LOA), intra-class correlation coefficient (ICC), within-subject coefficient of variation (wCOV) and Bland–Altman analysis for SUV and volumetric parameters. Lesions were stratified by size (>1 cm3 and >1.5 cm3) to assess the impact of lesion volume cut-offs on measurement variability. Results: A total of 297 lesions were analyzed, including 191 lesions > 1 cm3 and 161 lesions > 1.5 cm3. Test–retest variability was higher in smaller lesions, with narrower LOA and lower wCOV for larger lesions. SUVmax and SUVmean exhibited lower variability than SUVtotal and lesion volume. The 95% LOA for SUVmax ranged from −33.81% to +38.02% for all lesions, improving to −31.82% to +31.01% for lesions > 1.5 cm3. Similar trends were observed for SUVmean, SUVtotal, and volume. Bland–Altman plots confirmed reduced variability in larger lesions, with no significant systematic bias. Conclusions: The test–retest repeatability of AI-assisted PSMA-PET/CT features varies by feature type, with semi-quantitative features demonstrating improved repeatability relative to volumetric features. Additionally, repeatability is influenced by lesion size, with larger lesions exhibiting greater reliability. These findings highlight the importance of lesion size-dependent thresholds in response assessment and variability-aware feature selection in prognostic models. Current algorithms may be better optimized for larger lesions and higher volumes of disease, with limitations remaining in the robust detection and segmentation of smaller/more subtle lesions. and Simple Summary: To date, the test–retest repeatability of lesion-level features derived from artificial intelligence (AI)-guided prostate-specific membrane antigen (PSMA)-PET lesion segmentation has not been systematically assessed. One reason for that is the lack of available data. We demonstrate that a unique test–retest dataset of PSMA-PET scans, i.e., paired scans of patients with metastatic prostate cancer obtained within one week of each other, provides a test bed for AI algorithms to demonstrate how repeatably they can identify and delineate tumors. The methodology we describe is a new means of assessing the validity of AI algorithms.
Resource type:: Article
Affiliation Label Tesim:: School of Medicine, Department of Radiology, and Department of Medicine
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/kavs-s569
Edition:: Publisher
Identifier:: PMCID: PMC13030691, PMID: 41893833, and DOI: https://dx.doi.org/10.3390/tomography12030038
ISSN:: 2379-139X
Journal Issue:: 3
Journal Title:: Tomography
Journal Volume:: 12
Keyword:: respiratory function tests, intramuscular innervation, color, skeletal muscle atrophy, cross-cultural perspective, molecular docking, gene family, Achiron classification, gender roles, public health, active–latent–relapse murine tuberculosis model, carboplatin, phototoxicity, bisexuality, oral health–related quality of life, maternal–fetal bond, Alzheimer’s disease, bioactive pigments, micronutrients, protein engineering, immunogenetics, discharge readiness, nursing, countermovement utilization ratio, herbal medicine, signaling pathway, joint species distribution modeling, light-induced retinal damage (LIRD), nitrate reductase, cell tracking, deep-fat frying, unknown herbs, recombinant protein, type-2 diabetes mellitus, pangenome analysis, perinatal palliative care, tract sealing, semaglutide, S-adenosyl-L-methionine, casein peptides, colorectal cancer, multi-omics, geographical location, aquaculture, cellulose nanocrystals (CNC), rehydration capacity, trivalent mRNA vaccine, functional compound, disease activity, Styrax, visual screening, respiratory virus, acute ischemic stroke, TgMORN1, severe asthma, hematological parameters, multimodal analgesia, ductus venosus, immune checkpoint inhibitor, Caco-2 cells, drug repurposing, physiology-guided microsurgery, inflammatory bowel disease, equids, pachychoroid, COPE, sudden cardiac death, HSV-1, microglia, adolescence, Single-Nucleotide Polymorphism (SNPs), PERMA, empathy, health disparities, hif, sleep deprivation, efficacy, TILs, dual-task gait, lipid oxidation, machine learning, serum neutralizing antibodies, calcium phosphates, vasculature, ultrasonography, Fanconi anemia pathway, risk behavior, class imbalance, myocardial dysfunction, pancreas, caffeoylquinic acids, verapamil, core–shell structure, hypereosinophilic syndrome, self-efficacy, risk assessment, airway remodeling, antibiotic resistance, expressive suppression, academic satisfaction, cellular and tissue response, differentiation, genital herpes, emulsion structuring, one-carbon metabolism, regurgitation, Contactin-1 (CNTN1), soybean, growth traits, food intake, social virtual environments, autism, immunity, fatty acid, PICRUSt, probiotics, nanoparticles, food safety, early childhood caries, antioxidant capacity, three-dimensional, secondary school, veterinary medicine, Afro-descendant population, flesh quality, cognitive reappraisal, thalamic tumor, inflammaging, plant seed, compliance, deoxynivalenol, 4AT, leukopoiesis-stimulating drugs, microbiome–hormone interactions, environmental policy, gatekeepers, Toxicodendron, test–retest, tray drying, dynamic tubulin microtubules, PPC program, motor skills, cytosol, GABA, ADHD, tin, salt stress, bioactive peptides (BPs), astrocytes, fatty acids, health equity, validation, beef cattle, Rho, Popperian animal, phytochemicals, neuromuscular compartmentalization, foodborne pathogen, chitosan, megakaryocyte, heterodimerized receptor, perceived stress, berries, choriocapillaris, genomic analysis, thermostability, Wanxi white geese, ultrasound imaging, secondary-related AML, second victim, RNFL, pediatrics, robotic-assisted bronchoscopy, laboratory experiment, clopidogrel, homodimerized receptor, thyroid nodules, theapigments, mental disorders, oppositional defiant disorder, lipidomic biomarkers, biomarker, health services, iron sulfur clusters, antioxidant activity, GCIPL, exploratory behaviour, EUS, whitening, lung diseases, buprenorphine, Medicaid enrollment, renicola, BLUPmrMLM, non-targeted metabolomics, synbiotics, farnesoid X receptor, quality of life, translational control, resource selection, Hovenia, pachychoroid pigment epitheliopathy, cytotoxicity, countermovement jump, interventional pulmonology, consideration of future consequences, necroptosis, healthy controls, nucleus, Saponaria officinalis, pharmacokinetic, organizational commitment, amyloid β, Arabidopsis thaliana, ETA and ETB receptors, PI3K/AKT signaling, epidemiology, body mass index, sorghum, postoperative pain, intestinal function, kidney diseases, SKS, fatty liver, lipase, VRChat, ambient storage, clinical outcome, psychosocial positive resources, ribosome biogenesis, Dermatophagoides pteronyssinus, antibiotics, pharmacokinetics, Maternal and Child Health Handbook, women’s microbiome, pain management, structure elucidation, badminton, inflammation, ventilator-associated pneumonia, growth-disease resistance trade-off, in vitro digestion, cool water, aroma, ursodeoxycholic acid, nurse–mother communication, exosome, healthcare performance indicators, HLA-DQB1 typing, HSV-2, education, intramuscular fat, seagrass, methotrexate, SARS-CoV-2, activity level, delegated care, CMOS, metallomics, adenoviral vectors, skin aging, health promotion, waste management, executive dysfunction, iron-induced gut dysbiosis, exploration level, pathology, fecal microbiota transplant, drugs of plant origin, canine, analytical methods, empathetic leadership, young carer, depression, Arabidopsis mutant, Castanea, ripening, adoption, bioactive peptides, microbiological quality, damage-associated molecular patterns, oxidative status, hyphenated techniques, prebiotics, interoception, hopelessness, job satisfaction, coping, LF-NMR, network pharmacology, extremely preterm infants, pigment epithelial detachment, metastasis, Populus, colon polyp, Transactional Model of Stress and Coping, DJ-1, sophomore, PAMAM and PLL dendrimers, life-limiting conditions, oxidative stress, colorectal surgery, genome-wide association study, gut microbiota, in vitro simulated digestion, well-being, anxiety, yeast-derived emulsifiers, p53, nutraceuticals, zebrafish (Danio rerio), flow experience, lung, ASA score, neuroprotection, strawberry tree leaves, health-promoting properties, PET features, neonatal hyperglycemia, demethylation, anthocyanins, in vivo, tryptophan metabolism, nanocellulose, teachers, solid-state nanopores, FISH, rotavirus gastroenteritis, thalamocortical networks, hypofractionated IMRT, sickle cell disease, immunometabolism, religiosity, cat roaming, retina, low-fat cake, miRNAs, mutagenesis, reactive oxygen species (ROS), eicosanoids, hypoxia stress, early detection, dogs, pyroptosis, starch digestion, social determinants of health, human study, extraction solvent, biodegradation, prevention, field cancerization, breast cancer, cell-mediated immunity, delirium, suicide, treatment resistance, QTL mapping, ET-1, licochalcone A, DNA repair pathways, environmental responses, interspecific associations, neurodiversity-affirming research, eye infection, ethnobotany, hydrolysable tannins, acute myeloid leukemia, mind–body relationship, pneumonitis, theory of mind, metformin, endovascular thrombectomy, cholesterol metabolism, battle-of-the-sexes, feed processing, zeaxanthin, breathing practice, digital pathology, work engagement, pulmonary fibrosis, extrusion, HNSCC patients, cell therapy, immunization, microwaves, liver fibrosis, lysophosphatidic acid, fibrosis, parental educational involvement, lactic acid bacteria (LAB), psychiatry, occupational health nursing, SCP, epithelial–mesenchymal transition (EMT), echocardiography, genomic context, meaning integration, precision medicine, protein lipoylation, zein, foodborne pathogens, MD simulations, implementation and dissemination science, protocatechualdehyde, nurses, catfish powder, edible herbs, cis-regulatory module, septic shock, aged, migraine, framework, angiogenesis, ventricular atrium, bio-accessibility, aortic valve annulus, Sihler’s staining, hilar chlangiocarcinoma (HC), cell-free DNA (cfDNA), movement behavior, docking, gender stereotypes, microbiota–gut–brain axis, neurodegenerative diseases, mental health, cytoskeleton neurodegeneration, multiple sclerosis, UV-C treatment, CNP, chronic inflammation, Th17 cell plasticity, postbiotics, photodynamic therapy, TaqMan, oleic acid, CT-guided biopsy, lysine, biosensors, acute appendicitis, clinical ethics, RSV, patient experience, mechanisms, proactive personality, life satisfaction, DCP, family medicine, Zhoushan Fishing Ground, exercise therapy, Spheniscus humboldti, DNA sequencing, freeze-drying, normal fibroblasts, orthopedic surgery, risk factors, mediation analysis, fibroblast-to-myofibroblast differentiation, flavivirus, therapeutic alliance, inference by exclusion, antisocial personality disorder, GPS tracking, scale development, young adult carer, metabolic parameters, myeloid, body image, macrophages, nanoparticle, Nicotiana benthamiana, telemedicine, teach-back, post-mortem study, Xrcc1, women’s health, Australian wildlife, N-acetylcysteine (NAC), psychological recovery, emotion intensity, implementation science, cigarette smoking, vitreous humor, lipoxins, Robinia, crumb texture, acute radiodermatitis, endo-polygalacturonase, sleep, skin barrier repair, gE2, glutamate metabolism, bronchoscopy, AI, neuroendocrine neoplasms, microstructure, EU countries, small-breed dogs, surgical outcomes, teaching, ultrasounds, lipid peroxidation, lung cancer, qualitative synthesis, abdominal aortic aneurysm, tumor suppressor genes, lactate shuttle, drugs of cytokine origin, tetracycline, winemaking practices, montmorillonite (MMT), complementary treatment, tramadol–lidocaine–ketamine, therapeutic strategies, cuproptosis, multielemental analysis, emotion, junior school students, m6A, Switzerland, vaccine, TGF-β1, prognosis, atherosclerosis, phosphatidic acid, insertion orientation, qualitative research, calcium, fermented composite juice, lamina cribrosa cells, epithelial–mesenchymal transition, emotional intelligence, intestinal barrier, microenvironmental regulation, texture profile, fat replacer, rehabilitation, composite materials, angiotensin II, RPE, eating disorders, sensitivity, longitudinal data, explainable AI, 2-methoxystypandrone, biofilm, oxysterol, platypus, benzothiophene, ribosome heterogeneity, E1A enhancer, NRF2/HO-1 pathway, redox buffering, mitochondrial metabolism, Japan, Alternaria toxins, aphasia, MLL, attention mechanism, DNA polymerase, refrigerated storage, DNA damage response, veterinary training, graphic novel, major late promoter (MLP), E3 promoter, fish community, dental home, co-production, retrospective review, gut–vaginal axis, mitochondrial ROS, high-grade ovarian cancer, trace elements, autotaxin, reflexive thematic analysis, carnitine, maternal misperception, fertility, wound healing, pediatric dentistry, lesbian, matrikines, energy metabolism, popliteus muscle, functional food, metabolic pathway, gut microbiome, mycotoxin, histologically normal, diosmectite, dietary exposure, Türkiye, Listeria monocytogenes, speech and language therapy, life-threatening conditions, pork, bile acid, muscone, QSAR modeling, dog, butterfly pea flower, severity of illness index, pneumothorax, environmental DNA, irritable bowel syndrome, Jining Grey goat, quail, ductal carcinoma in situ, toxic metals, eggshell morphology, environmental pathogen monitoring, noise reduction, sexual orientation, LIRD, glutathione, amino acid, medical mediation, homologous recombination repair, genetic predisposition, ipsilateral recurrence, ultrasound, protein hydrolysate, drugs of bone marrow origin, saccharification, growth monitoring, metabolites, healthcare practitioners, tension-type headache, immune function, sexual minorities (SM), neuroinvasion, Integration Host Factor, multiplex real-time quantitative PCR, functional foods, lysophosphatidic acid receptor, honey bees, insecticide residue, Zika virus, meat product, cross-sectional studies, far infrared radiation, transcriptional signature, emergency nursing, MRI scans, liraglutide, veterinary anesthesia, opioids, SAH, SOFA, participatory methods, PET/CT, glycemic index, aminoquinazolines, pregnancy, health-promoting effects, instant noodles, durvalumab consolidation, synthetic promoter, athletes, Nubian, suicide risk, intraventricular extension, endoscopy, Ca2+ signaling, MYBPC3 gene, rRT-qPCR, mental health care, cancer, cardiomyopathy, aged mice, culture, clinical psychology, clean label, lutein, nanomedicine, vascular smooth muscle cells, self-defining memory, ex situ conservation, Ki67 and PSMA PET/CT, gD2, OCT, children, Conservation of Resources Theory, absolute quantitative sequence, heterosexuality, DR Congo, microgreens, surface functionalization, political climate, chromosomal abnormalities, gene therapy, cell migration, antioxidants, practical rationality, forest musk deer (Moschus berezovskii), aggression and impulsivity, anthocyanin-protein interactions, immunotherapy, smell, product labelling, digital health, point-of-care testing, Wagyu beef, mesenchymal stem cell, saporin, tirzepatide, stereotypic behaviors, cross-professional comparison, maresins, heritage wheat, computational drug design, post-fermentation, dairy cattle, abusive supervision, platelet yield, SPR, 5′UTR, food wastage, academic level, social communication, molecular docking studies, protein level, human eye, exercise enjoyment, OCTA, whole-genome sequencing, treatment response, artificial intelligence, transgene expression, Müller glia, speckle-tracking, neuroinflammation, mitochondrial respiration, germline mutations, retinal regeneration, Sansui ducks, cortex, conference report, Awassi sheep, MASLD, squat jump, rumenitis, circular economy, self-control, sensory analysis, gut–brain interaction, amoxicillin, complex karyotype, focal loss, α-GalCer, carrot waste LAB fermentation, CREB3L1, safety, propensity score matching, exercise addiction risk, retinal microvascular changes, DXS gene expression, university students, posterolateral complex, domestic cats, spine apparatus, cell proliferation, qualitative, additive manufacturing, PSMA expression, Camellia sinensis, integrated laboratory diagnostics, patient care, pirfenidone, smoking cessation, ROS, obesity phenotypes, intracranial compliance, umbilical–portal–systemic venous shunt, Communication Accommodation Theory, immune dysregulation, image preprocessing, Eupalinolide B, drugs of microbial origin, IoT, selective imitation, blepharoconjunctivitis, suicide-related knowledge, metabolomics, latent class analysis, perinatal outcomes, bioaccessibility, biodegradable polymers, mercury species, physical activity, iNKT cells, zoonotic gastrointestinal protozoa, theranostic biomarker, aroma persistence, deep learning, anti-influenza H1N1, pre-emulsification, fluoride bioavailability, pressure pain threshold, human vascular smooth muscle, immunosenescence, Modified Vaccinia Ankara virus, siRNAs, pollution, fermented meat product, evidence translation, SAM, oral bioavailability, transient expression, multi-locus GWAS, adverse-risk, habitat selection, growth performance, child body size, national values, vascular dementia, overweight, information-seeking, forensic psychiatry, medical records, glaucoma, interferon signaling, gestures, wine preference, specificity, Parkinson’s disease, meta-analysis, antisolvent precipitation, stroke, vaccination, liquid chromatography, acquired skill, paclitaxel, perilla seed meal, lipid oxidation stability, lesion localization, house dust mite allergy, glia, host response, foodborne diseases, memory, employee engagement, support, compulsive exercise, Mendelian randomization, HLA-DRB1 typing, rheumatoid arthritis, dendrimer–boron clusters, cancer immunotherapy, chemical proteomics, teletherapy, exosomes, phytobezoar, meat products, copper homeostasis, preschool children, transcriptomics, glycoproteins gC2, trace minerals, internalization, fish diversity, biomarkers, antibacterial activity, neonatal intensive care, molecular dynamics, neutralizing antibodies, oral retention, resource availability, chemotherapy, homing, mice, vitamin D, rheology, multicopper oxidase, translocation speed control, fermentation microecology, social skills, influenza virus, academic delay of gratification, lavender by-product, dietary supplements, IL-17 signaling, renal fibrosis, biomaterials, gay, policy, mechanical hyperalgesia, multi-habitat, edible tree peony flower, methylmercury, redox signaling, dendritic spines, radiosensitivity, Alzheimer’s disease animal models, oral treatment, telerehabilitation, payoff asymmetry, yeast mannoproteins, anti-obesity therapy, Rho associated coiled coil forming protein kinase (ROCK), crossbreeding, molecular biology, Fusarium toxins, Ki-67, saponins, ASPECTS, performance, silver diamine fluoride, non-alcoholic fatty liver disease, candidate genes, perception, antioxidant, ferroptosis, imidazole-containing drugs, spirituality, rabbits, resolvins, ready-to-eat foods, compromise, health, nasopharyngeal swabs, genetic independence, obesity, emotion regulation, embodied cognition, adverse events, microbiome, particle size, methionine, ensemble learning, intercellular signaling, public relations, known odour, network phenotyping, nanopore clogging, diagnostic accuracy, pointing, mitochondrial dysfunction, skin health, mycobiome, ductal remodeling, PSMA-PET/CT, endogenous adenoviral promoters, neutrophil degranulation, scenario-based survey, matrisome, ICP-MS, virome, health claims, drugs of chemically pure origin, combined or multiplex RDT, mineral elements, known taste, anti-wrinkle, autopsy, emotional intensity, epigallocatechin gallate (EGCG), MYC, post-harvest microbial control, pharmacogenomics, sepsis, radiation therapy, plasmid conjugation, autism spectrum disorder, adolescents, university counselling, tumor stage, COVID-19, native flora, DNA polymerase beta, prevalence, serum, alternation, cone-beam computed tomography, Toxoplasma gondii, disease detection, exercise, Mycobacterium tuberculosis, tumor margin, N-acetylcysteine, vision transformer, extraction, Campylobacter, lncRNA sequencing, caries risk assessment, boron neutron capture therapy, comparative genomics, biodiversity, plasma biochemistry, dimercaprol, early childhood, G protein, tea polysaccharides, endotyping, ROP, calf, DNA global methylation, network-level fusion, valorization, emerging adults, Enterocytozoon bieneusi, egg quality, CMPS-SF, tumor microenvironment, timeliness, polyphenols, PRDX4, apoptosis, senescence amelioration, chlorogenic acid, anastomotic leak, Larimichthys crocea, home range, immunoassay, drainage, 8-oxo-dATP, artificial intelligence integration, base excision repair, shelf life, Fujian province, transfer learning, survival, incretins, drugs of thymic origin, juice extraction, red cabbage, emotions, cat, hydrogen peroxide elicitation, high-density genetic map, monoamine oxidase, Listeria spp., hydromorphone, low-dose fractionated radiation, HRS, MHC class II, virtual reality, simulation optimization, estrobolome, healthcare conflict, quiet quitting, target identification, molecular dynamics simulation, vaccines, sodium iodate, endoscopic ultrasound, heat stress, nitric oxide, exercise motivation, amino acids, yeast protein extracts, reactive oxygen species, and nanocomposite coating
License Label:: Attribution 4.0 International
ORCID:: , https://orcid.org/0000-0002-8315-6603, https://orcid.org/0000-0002-1621-6661, https://orcid.org/0000-0003-3372-6046, https://orcid.org/0000-0001-6753-3010, https://orcid.org/0000-0002-4138-2186, https://orcid.org/0000-0001-5230-7782, https://orcid.org/0000-0002-9906-5087, https://orcid.org/0000-0003-2780-5100, and https://orcid.org/0000-0002-8965-8129
Other Affiliation:: , Milton and Carroll Petrie Department of Urology, Icahn School of Medicine at Mount Sinai, New York, NY, USA, Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX, USA, Department of Nuclear Medicine, LMU University Hospital, LMU Munich, Munich, Germany, Department of Nuclear Medicine, Universitätsklinikum Carl Gustav Carus at the TU Dresden, Dresden, Germany, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA, and AIQ Solutions, Madison, WI, USA
Page Start:: 38
Person:: Wisniewski, Nicholas, Gorin, Michael A., Whang, Young E., Islam, Md Zobaer, Werner, Rudolf A., Pomper, Martin G., Bundschuh, Ralph A., Pienta, Kenneth J., Weisman, Amy, Perk, Timothy G., Markowski, Mark C., Rowe, Steven P., and Milowsky, Matthew I.
Publisher:: Multidisciplinary Digital Publishing Institute (MDPI)
Rights Statement Label:: In Copyright

39. Assessing Willingness to Pay for Genetic Testing Among Adults: A Cross-Sectional Study Using Data from the Omnibus Survey 2022

Title Tesim:: Assessing Willingness to Pay for Genetic Testing Among Adults: A Cross-Sectional Study Using Data from the Omnibus Survey 2022
Creator:: Navas, Angelo, Hendy, Lauren, and Roberts, Megan
Date of publication:: 2026
Abstract Tesim:: Background: Population genetic screening (PGS) serves an essential role in identifying individuals at higher risk for hereditary cancer and cardiovascular disease. Nevertheless, the current lack of insurance coverage for screening costs might pose a barrier to its adoption. Health systems might contemplate covering these test expenses, but individuals covered by Medicaid and Medicare may not qualify for cost-free screening due to constraints related to the Beneficiary Inducement Statute. Methods: A cross-sectional online survey was administered to 602 US adults in January 2023. Andersen’s model guided variable selection. An ordered probit model was deployed to explore the association between insurance type and willingness to pay (WTP) for PGS, controlling for demographic and healthcare characteristics. Results: Among the 602 respondents, 524 (87%) were included in our analysis. Over 70% (n = 373) of participants expressed WTP for genetic testing. A similar proportion of respondents with Medicare and Medicaid expressed WTP for screening (68%, and 70%, respectively). Insurance type was not significantly associated with WTP for genetic testing. Notably, lower trust levels and absence of family cancer history were associated with a lower probability of expressing high WTP compared to the reference categories (high levels of trust and having a family cancer history). Conclusions: WTP for genetic testing was not significantly associated with insurance type. Almost 30% of our sample were unwilling to pay for PGS, suggesting variability in WTP for PGS and adding to the limited literature on how individuals value genomic screening tests.
Resource type:: Article
Affiliation Label Tesim:: Eshelman School of Pharmacy and Division of Pharmaceutical Outcomes and Policy
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/m73m-re03
Edition:: Publisher
Identifier:: PMCID: PMC13027871, PMID: 41893022, and DOI: https://dx.doi.org/10.3390/jpm16030154
ISSN:: 2075-4426
Journal Issue:: 3
Journal Title:: Journal of Personalized Medicine
Journal Volume:: 16
Keyword:: Cognitive workload, reactive oxygen species, neural networks, source-oriented exposure, Cancer, corneal tissue, dysphagia, sputum, primary school children, pediatric vertigo, sedative agent, psychiatry, twin transformation, communicable diseases, sex, Medicine, calcium intake, antibiotic prophylaxis, sleep, trace-element load, myristic acid, human growth hormone, female reproduction, illumination correction, Video assisted surgery, pediatric ischemic stroke, smear layer, tissue engineering, rootstocks, wildlife caregiving, Blood brain barrier (BBB), older adults, body composition, K-medoids clustering, outcome measures, crop resilience, surveillance study, systemic inflammation, oral motor rehabilitation, TCRAT, ATG5, COPD, cardiothoracic surgery, redox signalling, brain cooling, central sensitization, migrant health, Argentina, immune microenvironment, buffers, carotid artery, Trade openness, positive and negative affect, hydrates, PTSD, PED, drying-rewetting cycles, Tunisian Arabic, soft tissue tumors, Monte Carlo multi-track chemical modeling, antioxidant, Physics, Cell biology, phylogenetic analysis, colorectal cancer, Newcastle disease virus (NDV), cellulose nanofibers, ROS1, anti-wetting modification, extracellular vesicles, diagnostic imaging, Energy systems, children, landfill leachate, fracture, proactive health behaviors, psychometric properties, radiolytic oxygen depletion (consumption) (ROD), Drug delivery system, episiotomy, microbiota, young people, Mental health, Molecular mechanism of gene regulation, influenza vaccine manufacturing, nursing, Balb/c mice, P-glycoprotein, machine learning, radiation track structure, white blood cell count, foodstuff, multidrug resistance reversal, Stress, aluminum hydroxide gel, Electrochemistry, physicochemical stability, concomitant vaccination, heterogeneity, personalized COPD management, menopause, lipid profile, Transformer, scoping review, psychotherapy, stable isotope and radiocarbon, group-based trajectory modeling, manganese dioxide, cirrhosis, Escherichia coli, antimicrobial activity, systematic reviews, MACC1, brain cortical thickness, zinc oxide nanoparticles, human and veterinary medicine, cellulose synthases, encoding scheme, cylindrospermopsin, burn, EQUATOR network, Microbiology, CGC, synthetic musks, Koch’s postulates, oxidation stability, Electrophoretic Displays (EPDs), neglected tropical disease, pelvis, total IgE, prostate cancer, vagus nerve, functional pituitary tumors, pandemic influenza, tocilizumab, conscious sedation, wound dressings, twenty-four-hour movement behaviors, culture, NMR, retroperitoneal, pleckstrin homology domain, nephrology, Climate disaster, latent class analysis, Klebsiella pneumoniae, alirocumab, Buenos Aires, rehabilitation, antidepressants, adipocytes, BDNF, Research design, case series, gellan gum, salicylic acid, NFκB, signal transduction, low-intensity ultrasound, aging, associated vaccine, super-resolution and recognition of text images, vaginal delivery, SNR efficiency, metabolomics, animal study, whole-genome sequencing (WGS), sensorimotor integration, synthetic vitamin D analogs, Trachystemon orientalis (L.) G. Don, gender-specific healthcare, microbiome, stomatal conductance, simulation frame, type-II diabetes, medicine, vessel-selective, Distraction osteogenesis, Anticancer activity, place-based approaches, CVB3, ST11, source-separated urine, multi-modal diagnosis, fatty acids, telemedicine, GABA, silicosis, artificial intelligence, age-related, Australia, vestibular disorders, vaccination coverage, immunosuppression, male infertility, blepharophimosis, ptosis, and epicanthus inversus, child, capping agents, X-rays, functional food, Therapy, shooting performance, children and adolescent, glycosyltransferase family 2, rare diseases, treatment patterns, polycyclic aromatic hydrocarbons, lipid nanoparticles (LNPs), sustainable nanomaterials, endoscope, AI-assisted learning, skin, Copy number variation, secretion, Electrochemical materials science, evaluation, macrophage activation, meta-analysis, chest X-ray, migration, cryoprotectants, Streptococcus mutans, organotypic brain culture, Performance analysis, kitchen ventilation, site 1-binding helix, mtHsp70, stabilizers, Abdominal wall reconstruction, cyanotoxin, sex differences, volatile organic compounds (VOCs), Prediction, ferronickel slag, health risks, Transcriptomics, copper and iron metabolism, brain networks, excision, eosinophilia, type 2 diabetes mellitus, Echium amoenum, hospitalization, melanoma, autoimmune disease, emotion regulation, Ediacaran, targeted temperature management, neurogenetics, vaccine delivery, cost-benefit analysis, sodium alginate, sexual harassment, Dynamical system, blood–retinal barrier, inpatients, formulation optimization, surgical wound infection, neonatal cardiac surgery, Biochemistry, gastrointestinal cancer, Weissella cibaria, Agriculture, myolipoma, Kazakhstan, alkali-activated slag, men’s health, PTH, dexmedetomidine, metastatic, chronic pain, implant, screening, solvent removal, Enterobacterales, infectious laryngotracheitis virus (ILTV), male osteoporosis, Stem cells research, etiology, hadrontherapy, dispersive solid-phase extraction, physical activity, phenolic compounds, glutathione, vascular endothelial growth factor, systemic lupus erythematous, innate immunity, cytokine production, talent development, Molecular mechanism, survey, Depression, jamming, lung ultrasound, Uniportal-VATS, racial harassment, co-culture fermentation, antibiotics, sensory re-weighting, ascorbate–glutathione cycle, neonatal hypoxia, purpose, hydrogels, otitis externa, biomarker development, problem-solving, allergic rhinitis, MRI, Mesh reinforcement, exercise, preschool, antegrade perfusion, Doushantuo formation, fibrosis, lactic acid bacteria, urachus, fluid overload, survival, microglia, immunogenicity, soft tissue attachment, DOC, Nerve conduction velocity (NCV), seafarers, orthodontics biomechanics, anti-neutrophil cytoplasmic antibody (ANCA), Small for gestational age, meningohypophyseal trunk aneurysm, spatio-temporal variation, sedation, solution-focused brief counseling, eating disorders, autochthonous cases, Granger causality analysis, matching-adjusted indirect comparison, Kinematics, aged, treatment, spine surgery, cold chain, clinical assessment, stress, radiation, digital transformation, cardiac arrest, Cardiovascular risk, antioxidants, older persons, diabetic neuropathy, burnout, peroxiredoxin–thioredoxin, flavonol, Functional aspects of cell biology, total coronary revascularization, spermatogenic failure, bladder, Gross domestic product, qualitative data, Low-speed motor vehicle collisions, subjective well-being, profiles, mediastinum, obesity, risk factors, bone anabolic therapies, Variant interpretation, pain measurement, LSNS-6, vessel-encoded arterial spin labeling (VEASL), PCSK9, peritoneal dialysis, Neck injury, ROS, interviews, oomycete pathogenesis, natural product chemistry, alginate, neonatal intensive care, postpartum, blaNDM-5, virtual reality, intraoperative fluorescence, transcriptional regulation, psychological flexibility, vaccination, oxidative stress, immune checkpoint inhibitors (ICIs), industrial processing of material, Evolution, freeze-drying, atomic absorption spectroscopy, growth charts, behavioral adaptation, foreign language learners, prevention, Developmental biology, Body dysmorphic disorder, multimorbidity, safety behaviors, Strongyloides stercoralis, coherence, Lipoprotein(a), core genome analysis, Complex reconstruction, Physiology, anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV), nociplastic pain, blepharoptosis, palatal lift prosthesis, herpes zoster vaccine, cross-sectional research, Psychology, lyophilization, High-risk pregnancy, Chicken meat production, flavonoids, pathogen, Mitochondrial unfolded protein response, urban WWTPs, development strategies, Sabin-strain inactivated poliovirus vaccine, aerobic training, DTR, amyloid β-Protein, cooking fuel, NF-κB pathway, computer calculations, SIX transcription factors, PTG, MgO, Obstetrics, multilocus phylogeny, mental health, vaccine effectiveness, fatigue, diagnostic errors, Lubben Social Network Scale, green healthcare, antimicrobial resistance, shotgun metagenomic sequencing, non-small cell lung cancer, MDSC, dose–response, 100 Days Mission, CCL12, soil CO2 pulsesand emissions, metabolic reprogramming, pathophysiology, growth monitoring, gut-kidney axis, selective nuclear receptor modulators, developmental language disorder, hormones, avian influenza, grapevine, endotoxin tolerance, influenza vaccine, urinary VOC metabolites, soft robotics, influenza, Vitamin D Receptor (VDR), test-negative design, migraine, Systems biology, Alzheimer’s disease, fermentation, mathematical giftedness, dental caries, digital orthodontics, Internal medicine, perineal lacerations, adipokines, gut-liver axis, cardiopulmonary bypass, Diet, Healthcare staff, healing, copper nanoparticles, humor, multi-sensory stimulation, generalized structural equation modeling, VTE prevention, membrane distillation, Omics, Ceramiales, surveys and questionnaires, gastrointestinal diseases, dengue/complications, pulmonary embolism, extract, chemosensitization, coping styles, selectivity index, retinal vein occlusion, hickory nut oil, Bioengineering, learner agency, nitrous oxide, neuroinflammation, Adolescents, Rhizopogon roseolus, cyclodextrin, Reporting standards, anticariogenic, epidemiology, Kisantu, vaccine platforms, vaccine, deep learning, cellular water radiolysis, Chicken meat consumption, COM-B model, Energy engineering, hydrocarbon stapling, Lipoproteins, cancer immunotherapy, stereotactic brain biopsy, exoscope, oncogenesis, biomedical health trainees, SARS-CoV-2, systematic review, JS-K, bloodstream infection, Materials science, gender, empathy, MAPK signaling, Cognitive biotypes, endoscopic disease detection, wellbeing, temperate Europe, attitudes, incivility, adenoid hypertrophy, hybrid genome assembly, IFN-α, multidrug resistance, Pedicled thigh flap, marine lectins, social support, urachal signet-ring cell carcinoma, mechanical properties, influencing factors, essential oils, MCF-7 cell line, automated path planning, External fixation, family, water-use efficiency, KNHANES, neonatal puppy, wastewater surveillance, photobiomodulation, low-dose naltrexone, chronic liver disease, root canal microbiota, Cassano classification, Improved Whale Optimization Algorithm-based Multi-scale Fusion Attention enhanced SwinIR (IWOA-MFA-SwinIR) model, pneumoconiosis, neuroimmune crosstalk, mercury, XDR, Robotic assisted surgery, Dynamic Time Warping, green synthesis, elderly, Artificial intelligence, model robustness, mouse, antiviral activity, Electromyogram (EMG), cytotoxicity, Magnetic materials, abiotic stress, microbiome-mitochondria axis, pediatric, NADPH, thymus, cooking duration, ovary, non-typhoidal Salmonella, glutaredoxin, antioxidant potential, thrombosed aneurysm, Automated tools, linear energy transfer (LET), Autophagy, Professional Achievement in Mathematics (PAM), individual differences, drylands, Animal science, whole genome, circadian disruption, biomarker discovery, ESKAPEE pathogens, expanded dengue, pediatric dentistry, willingness to pay, medical postgraduate students, oral appliance, Cellular neuroscience, neuronal development and homeostasis, refrigerated storage, indocyanine green, measurement equivalence, vaccinology, DEIM, bioactivity, metabolic pathway, endoscopic transsphenoidal surgery, mcr-1.1, folliculogenesis, Apolipoprotein B, thrombectomy, caries, carbapenem-resistance, AlphaFold-multimer, dementia, IER2, Biotechnology, follow-up, treatment outcome, glioblastoma, exo-metabolome, recombinant viral protein rVP1, Female, Entomology, multimodal large language model, medical specialty, octogenarians, dizziness, Sleeve resection, polyphenol, mRNA vaccines, China, QE duration, irrigation, pembrolizumab, lipid peroxidation, EPR spectroscopy, sensitization, polychlorinated biphenyls, exacerbation, proliferation, ligand-dependent transcription, orthodontics, locally advanced, human growth hormone receptor, Health informatics, lactic acid bacteria (LAB), immunomodulation, neuroplasticity, Hernia repair, mitochondria, ST307, finite element analysis, population genetic screening, simulated treatment comparison, pulmonary tuberculosis, activities of daily living, global health security, venous thromboembolism, histoplasma, HMGB1, Cardiac arrest, supercapacitors, Molecular biology, beneficiary inducement statue, TGF-β proteins, distance-based effectiveness, vaccine strains, Extreme weather, 3D model, implementation science, vascular territory, Machine learning, tumor microenvironment (TME), rheumatoid arthritis, MSWI-FA, Engineering, real world, endovascular coiling, basketball, metastasis, infectious complications, particulate matter, N-gram chi-square feature selection, FLASH radiotherapy, biological sciences, Ilizarov technique, AMH, velopharyngeal dysfunction, DRC, multi-class detection, gas exchange, Osteotomy, peptide antagonist, Biodevices, multimodal analysis, pediatric disease diagnosis, ECG, Health sciences, IgG4-related disease, organophosphate esters, pandemic preparedness, cognitive dysfunction, Rear impact, NSCLC, atypical symptoms, periodontitis, swine, multimodal pain management, Arabic validation, Immune response, oral health, abducens nerve palsy, smoking, Crohn’s disease, sport participation, samplingstrategy, hip, glucocorticoids, vertebral artery, process data, significance, Jordan, ApoBAsians, cognition, end-stage kidney disease, pain, distribution patterns, neoplasm, ionizing, mRNA-LNPs, ketamine, microstructure, bacitracin, macrophage polarization, Toll-like receptor 4, microcystins, cancer stem cells, inflammation, Electrical engineering, endo-metabolome, chemical yields (G values), deep vein thrombosis, organochlorine pesticides, adverse event following immunization, 6-methoxykaempferol, long-term storage, surgical intervention, Dusp15, psilocybin, early identification, extracellular, volunteering, real-world evidence, inactivated combined vaccine, cognitive function, brain injury, digital twins, perspective-taking, retrospective studies, microaggressions, Coffee, genome stability, dental treatment, bone mineral density, 3D scanning, etidronate, Cardiovascular medicine, endocrinology, adolescence, pomegranate, wound analysis, child development, robotic surgery, 23-valent pneumococcal polysaccharide vaccine, Anterolateral thigh flap, adoptive immunotherapy, NLRP3, Anxiety, coinfection, co-morbidity, helix-12 conformational change, meaning in life, materials synthesis, pro-AMH, Clustering, health insurance, Cancer systems biology, resistance training, 16S rRNA gene sequencing, Neuroscience, Reproducibility of results, industrial chemistry, oral mucosa, Surgical education, pulmonary edema, public health, emotional intelligence, Monte Carlo, IncHI2, carbon nanotubes, neurology, kidney biopsy, technical bottleneck, Perceptual bias, histomorphometry, Medical specialty, chemotherapy, PEDV, item response theory, TP53, sustainability, chronic apical periodontitis, entomology, aerogels, self-confidence, diabetic nephropathy, acute kidney injury, Optix/SIX3, influence of sex, vaccine safety, steam curing, behaviour, mycobacteria, One Health, Biological sciences, childhood, estrogen, type 1 diabetes mellitus, Microenvironment, outcomes, computer-aided diagnosis, central aortic cannulation, augmented reality, postural control, dengue, social media, helical peptide, alphaherpesvirus, AI, Cross-sectional study, ammonia nitrogen recovery, hypercalcemia, digital health, Lipids, organophosphate pesticides, Lp(a), medical image diagnosis, translational neuroscience, cetuximab, multiple sclerosis, brain functional connectivity, Bone transport, Rhodophyta, tuberculosis, ST258, health sciences, genome editing, psychedelic treatment, ESBL, surface electromyography, cancer, dermocosmetic, ultrasonography, endocrine disruptors, speciation, Colorectal Cancer, astrocytes, palatal augmentation prosthesis, midazolam, in situ forming gel, canine ear microbiota, carcinoma, brominated flame retardants, incidence, immune-enhancement, neuronavigation, Diabetic cardiomyopathy, personalized management strategies, root canal, sampling frequency, social isolation, and Healthcare workers
License Label:: Attribution 4.0 International
ORCID:: https://orcid.org/0000-0002-9036-4486 and
Other Affiliation:
Person:: Navas, Angelo, Hendy, Lauren, and Roberts, Megan
Publisher:: Multidisciplinary Digital Publishing Institute (MDPI)
Rights Statement Label:: In Copyright

40. A Unique Insertion Loop Facilitates Tight NAD+ Binding in Nicotinoprotein: Insights from In Vitro Loop Engineering and In Silico Studies

Title Tesim:: A Unique Insertion Loop Facilitates Tight NAD+ Binding in Nicotinoprotein: Insights from In Vitro Loop Engineering and In Silico Studies
Creator:: Ikebukuro, Kazunori, Okuda-Shimazaki, Junko, Yanase, Takumi, Xue, Houcheng, Sode, Koji, Miura, Daimei, Tsugawa, Wakako, Kawai, Haruka, and Asano, Ryutaro
Date of publication:: 2026
Abstract Tesim:: Nicotinoproteins are a group of NAD+-dependent dehydrogenases that bind NAD+ tightly and catalyze reactions without using free NAD+. In this study, we investigated the role of the unique insertion loop in nicotinoproteins. Carveol dehydrogenase (CADh), a short-chain dehydrogenase/reductase (SDR) nicotinoprotein, and β-hydroxybutyrate dehydrogenase from Alcaligenes faecalis (AfBHBDh), a non-nicotinoprotein counterpart, were used as model enzymes. An insertion loop-deleted mutant, CADh Δ39–49, was constructed. An insertion loop from Mycobacterium paratuberculosis CADh (MpCADh) was introduced into AfBHBDh to generate the two mutants. The results showed that CADh Δ39–49 lost NAD+ tight binding capacity and could not utilize free NAD+. In contrast, the AfBHBDh mutants showed no dye-mediated dehydrogenase activity. Moreover, the KM and KD values for NAD+ were higher than those of the wild-type enzyme. Docking simulations revealed a stronger binding affinity between NAD+ and the mutants than with the wild-type AfBHBDh. Taken together, these results suggest that the insertion loop interferes with NAD+ entry into the active site of the enzyme while creating a more energetically favorable binding environment. This loop is necessary but alone is insufficient to achieve NAD+ tight binding. This study deepens understanding of NAD+ binding in SDR nicotinoproteins and provides insights for SDR enzyme engineering.
Resource type:: Article
Affiliation Label Tesim:: School of Medicine and UNC/NCSU Joint Department of Biomedical Engineering
Type:: http://purl.org/dc/dcmitype/Text
DOI:: https://doi.org/10.17615/dsjw-7631
Edition:: Publisher
Identifier:: PMCID: PMC12985038, DOI: https://dx.doi.org/10.3390/ijms27052367, and PMID: 41828585
ISSN:: 1422-0067
Journal Issue:: 5
Journal Title:: International Journal of Molecular Sciences
Journal Volume:: 27
Keyword:: preference, laser in situ keratomileusis, B pillar, pediatric to adult care, bridge therapy, ATP synthesis, cholelithiasis, Lebanese population, nutrition, corneal biomechanics, ΔΔCq, neurovascular resilience, dementia, esophageal atresia, partial nephrectomy, Icaritin, molecular profiling, short-chain fatty acids, hot spring, refractories, amyloid-β, modeling immune patterns, tool wear and surface roughness, review, gate-all-around (GAA), bump height, metallurgical quality, measurement uncertainty, sunlight exposure, super-resolution, psychosocial health, ascending aortic aneurysm, albumin, inhaled corticosteroid therapy, responsivity, pulmonary tuberculosis, gene profiling, Rigenera, perceived health, renal cell carcinoma, alpha-synuclein, mitochondria, ischemia–reperfusion injury, older adults, MPS, surface chemistry, estradiol (E2), chronic wounds, ferric derisomaltose, peripheral screw, neural networks, spinal cord injury, Ménière’s disease, psoriasis, virtual reality, LED, residual stress, stroke, bone mineral density, chronic intermittent hypoxia, intergenerational commensality, iron deficiency anemia, virtual monoenergetic imaging, cross-sectional study, cervicovaginal microbiome, plasma treatment, molecular diagnosis, sexual function, automated image analysis, feasibility study, canine, saline–alkali adaptation, transition readiness, electrocardiogram, TRAMP, Parkinson’s disease dementia, white blood cell count, home care, phylogenetics, infections, wear, TCA cycle, Calyptratae, cryogenic, pediatric renal tumors, dementia with Lewy bodies, photon-counting detector computed tomography, Dieulafoy’s disease, cracks, HER2-low, trachea, superaged societies, Rett syndrome, mitosis, PCSK9 inhibitor, dietary habits, adiponectin, vHIT, pH, immunotherapy, multi-omics analysis, subjective well-being, bevacizumab, functional difficulty, genetic counseling, malignancy, antifungal agents, stress responses, protein C, neuromodulation, cancer survivorship, 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disease, Human–Computer Interaction (HCI), patient safety, gut–liver axis, redo-TAVR, EN AW 7075, convolutional neural network (CNN), physicians, neurite outgrowth, 3D printing, P2X7R, welding heat input, lifetime management, PARP1, 3D testbeds, occipital neuralgia, precipitation, COSMIN methodology, suicide risk, hot-press forming, visual acuity, machine learning, aging, transcatheter aortic valve replacement, corneal resistance factor, 3D staining and imaging, delays, Lactobacillus-to-anaerobe log-ratio, cardiotoxicity, COPD, bone metastasis, recurrent admission, polycrystalline diamond, root length, HER2-zero, diabetes mellitus, living donor liver transplantation, species abundance, proteostasis, monogamous, β-synuclein, immunogenetics, reactivation from latency, piezoelectric bimaterials, cephalometric analysis, growth-defense trade-off, copolymers, HFNC, pathology modeling, myopia, ageing population, lung metastases, atogepant, computed tomography, medical education, VEGF-TKI, upper tract urothelial carcinoma, atrial septal defect, glenoid baseplate, triclosan, graft failure, non-small cell lung cancer (NSCLC), metabolic disorder, Krebs cycle, DSC, PDS, Healthgrades, crisis, tetraantennary glycans, non-operative management, stiffness degradation, Inconel 600, meta-analysis, Wilms’ tumor, neutrophil percentage, antioxidant peptide, uremic toxin clearance, 25-hydroxyvitamin D, geranyl methacrylate, head and neck cancer, long non-coding RNAs, bath, inborn errors of metabolism, porous structure, beta-blockers, nitrogen concentration, interaction design framework, Squeeze-and-Excitation, elective surgery, pediatric leukemia, suicidal ideation, artificial intelligence, HSV-1, mAbs, postoperative pain, alkenes, drone, guanine nucleotide exchange factor, fever, facilitators, TiCl4 refining, safe zones, infraclavicular region, T47D, multiple system atrophy, AECOPD, swept-source OCT, lifestyle factors, anti-VEGF, vestibular physical therapy, percutaneous closure, phosphatase of regenerating liver-3, molecular docking, hybrid-type glycans, hidden blood loss, laparoscopic living donor hepatectomy, graft-versus-leukemia, thermal decomposition, stage IV melanoma, AI, CAPD scale, kidney replacement therapy, CT-osteoabsorptiometry, choroidal melanoma, skeletal muscle, caregiver, RECIST 1.1, somatization, organic reagent, reaction mechanism, Origanum vulgare, grey relational analysis (GRA), tumor-associated macrophages, intensive care unit-acquired weakness, adenoid cystic carcinoma, steel C45, blood–brain barrier, chronic intermittent ethanol, M2 macrophages, Cited2, estetrol (E4), trunk, real-time PCR (qPCR), cerebellar mutism, BEVAR, metabolism, cold rolling, shear band, ROP, ocular graft-versus-host disease, integral transformation, achilles tendon, tibial footprint, fungal infections, [68Ga]PSMA-11, PAED scale, regeneration, multi-channel image fusion, interatrial block, environmental stressor, laser shock peening, divergence time estimation, osteoporosis, ADCs, skin, CGRP receptor antagonists, microvascular dysfunction, functionalization, neurosurgery, lymphatic invasion, ZW, visual large language models, MPMCA, pilot RCT, cisplatin, seawater, vestibular migraine, pluripotency and cell fate regulation, CT abnormalities, thermo-mechanical properties, hydrogen embrittlement susceptibility, SIRT6, patient-specific, anticoagulation, Akt, diagnostic modeling, glia, rheological behavior, adenocarcinoma, picromerite, patient-reported outcomes, spatial attention module, tissue engineering, induced pluripotent stem cells, deep learning, porosity, aluminum, short-chain dehydrogenase/reductase, total blood loss, intellectual disabilities, reverse shoulder arthroplasty, habitat fragmentation, sleep apnea, spine, cryo-electron tomography, autophagy, epigenetics, echocardiography, TBS, glucocorticoid receptor (GR), zongertinib, culturally tailored interventions, kidney fibrosis, root fungal endophyte, nickel-modified biocarbon, wound healing, bimetallic catalyst, high-fat diet, guidelines, myokine, bulk-GeOI, dry eye disease, concrete aggregates, prognosis, sarcoma, mechanical enhancement, quantification, thermal simulation, thermophysical properties, sustainable machining, NACT, infection, pulmonary arterial hypertension, ceralasertib (AZD6738), epiboly, audiovisual digital phenotyping, liver, peripheral nerve block, rural older adults, nerve injury, densenet121, glycome, endoscopic laser ablation, nursing homes, histological healing, micronuclei, and chronic GvHD
License Label:: Attribution 4.0 International
ORCID:: https://orcid.org/0000-0003-2838-0562, https://orcid.org/0000-0001-5802-7124, https://orcid.org/0000-0003-2284-6499, , https://orcid.org/0000-0002-9833-2091, https://orcid.org/0000-0001-6299-5267, and https://orcid.org/0000-0001-6795-8377
Other Affiliation:: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan and
Person:: Ikebukuro, Kazunori, Okuda-Shimazaki, Junko, Yanase, Takumi, Xue, Houcheng, Sode, Koji, Miura, Daimei, Tsugawa, Wakako, Kawai, Haruka, and Asano, Ryutaro
Publisher:: Multidisciplinary Digital Publishing Institute (MDPI)
Rights Statement Label:: In Copyright