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1771. Sustainable Cost Models for mHealth at Scale: Modeling Program Data from m4RH Tanzania

Title Tesim:
Sustainable Cost Models for mHealth at Scale: Modeling Program Data from m4RH Tanzania
Creator:
Mangone, Emily R., Lasway, Christine, Agarwal, Smisha, Zan, Trinity, Orkis, Jennifer, Van Beijma, Hajo, Karam, Robert, and L'Engle, Kelly
Date of publication:
2016
Abstract Tesim:
BackgroundThere is increasing evidence that mobile phone health interventions (“mHealth”) can improve health behaviors and outcomes and are critically important in low-resource, low-access settings. However, the majority of mHealth programs in developing countries fail to reach scale. One reason may be the challenge of developing financially sustainable programs. The goal of this paper is to explore strategies for mHealth program sustainability and develop cost-recovery models for program implementers using 2014 operational program data from Mobile for Reproductive Health (m4RH), a national text-message (SMS) based health communication service in Tanzania.MethodsWe delineated 2014 m4RH program costs and considered three strategies for cost-recovery for the m4RH program: user pay-for-service, SMS cost reduction, and strategic partnerships. These inputs were used to develop four different cost-recovery scenarios. The four scenarios leveraged strategic partnerships to reduce per-SMS program costs and create per-SMS program revenue and varied the structure for user financial contribution. Finally, we conducted break-even and uncertainty analyses to evaluate the costs and revenues of these models at the 2014 user volume (125,320) and at any possible break-even volume.ResultsIn three of four scenarios, costs exceeded revenue by $94,596, $34,443, and $84,571 at the 2014 user volume. However, these costs represented large reductions (54%, 83%, and 58%, respectively) from the 2014 program cost of $203,475. Scenario four, in which the lowest per-SMS rate ($0.01 per SMS) was negotiated and users paid for all m4RH SMS sent or received, achieved a $5,660 profit at the 2014 user volume. A Monte Carlo uncertainty analysis demonstrated that break-even points were driven by user volume rather than variations in program costs.ConclusionsThese results reveal that breaking even was only probable when all SMS costs were transferred to users and the lowest per-SMS cost was negotiated with telecom partners. While this strategy was sustainable for the implementer, a central concern is that health information may not reach those who are too poor to pay, limiting the program’s reach and impact. Incorporating strategies presented here may make mHealth programs more appealing to funders and investors but need further consideration to balance sustainability, scale, and impact.
Resource type:
Article
Affiliation Label Tesim:
Gillings School of Global Public Health and Kenan-Flagler Business School
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/jbp2-qw35
Identifier:
Publisher DOI: https://doi.org/10.1371/journal.pone.0148011, PMID: 26824747, Onescience id: 312275df5dbeb4bb1b1dbd4ca46d7b7470a13fc5, and PMCID: PMC4733101
ISSN:
1932-6203
Journal Issue:
1
Journal Title:
PloS One
Journal Volume:
11
Keyword:
Tanzania, Text Messaging, Telemedicine, Cost-Benefit Analysis, Contraception Behavior, Condoms, Adult, Male, Female, Models, Economic, Humans, Family Planning Services, Contraceptive Agents, and Monte Carlo Method
Language Label:
English
ORCID:
Other Affiliation:
FHI 360, Johns Hopkins Center for Communication Programs, Text to Change, and School of Nursing and Health Professions; University of San Francisco
Page Start:
e0148011
Person:
Mangone, Emily R., Lasway, Christine, Agarwal, Smisha, Zan, Trinity, Orkis, Jennifer, Van Beijma, Hajo, Karam, Robert, and L'Engle, Kelly
Rights Statement Label:
In Copyright
Source:
h415pg56s

1772. Structural Determinants of Antiretroviral Therapy Use, HIV Care Attendance, and Viral Suppression among Adolescents and Young Adults Living with HIV

Title Tesim:
Structural Determinants of Antiretroviral Therapy Use, HIV Care Attendance, and Viral Suppression among Adolescents and Young Adults Living with HIV
Creator:
Fernandez, Maria I., Bauermeister, Jose A., Bruce, Douglas, Hightow-Weidman, Lisa B., Kahana, Shoshana Y., and Jenkins, Richard A.
Date of publication:
2016
Abstract Tesim:
BackgroundThe authors examined associations between structural characteristics and HIV disease management among a geographically diverse sample of behaviorally and perinatally HIV-infected adolescents and young adults in the United States.MethodsThe sample included 1891 adolescents and young adults living with HIV (27.8% perinatally infected; 72.2% behaviorally infected) who were linked to care through 20 Adolescent Medicine Trials Network for HIV/AIDS Interventions Units. All completed audio computer–assisted self-interview surveys. Chart abstraction or blood draw provided viral load data. Geographic-level variables were extracted from the United States Census Bureau (e.g., socioeconomic disadvantage, percent of Black and Latino households, percent rural) and Esri Crime (e.g., global crime index) databases as Zip Code Tabulation Areas. AIDSVu data (e.g., prevalence of HIV among youth) were extracted at the county-level. Using HLM v.7, the authors conducted means-as-outcomes random effects multi-level models to examine the association between structural-level and individual-level factors and (1) being on antiretroviral therapy (ART) currently; (2) being on ART for at least 6 months; (3) missed HIV care appointments (not having missed any vs. having missed one or more appointments) over the past 12 months; and (4) viral suppression (defined by the corresponding assay cutoff for the lower limit of viral load at each participating site which denoted nondetectability vs. detectability).ResultsFrequencies for the 4 primary outcomes were as follows: current ART use (n = 1120, 59.23%); ART use for ≥6 months (n = 861, 45.53%); at least one missed HIV care appointment (n = 936, 49.50); and viral suppression (n = 577, 30.51%). After adjusting for individual-level factors, youth living in more disadvantaged areas (defined by a composite score derived from 2010 Census indicators including percent poverty, percent receiving public assistance, percent of female, single-headed households, percent unemployment, and percent of people with less than a high school degree) were less likely to report current ART use (OR: 0.85, 95% CI: 0.72–1.00, p = .05). Among current ART users, living in more disadvantaged areas was associated with greater likelihood of having used ART for ≥6 months. Participants living in counties with greater HIV prevalence among 13–24 year olds were more likely to report current ART use (OR: 1.32, 95% CI: 1.05–1.65, p = .02), ≥6 months ART use (OR: 1.32, 95% CI: 1.05–1.65, p = .02), and to be virally suppressed (OR: 1.50, 95% CI: 1.20–1.87, p = .001); however, youth in these areas were also more likely to report missed medical appointments (OR: 1.32, 95% CI: 1.07–1.63, p = .008).ConclusionsThe findings underscore the multi-level and structural factors associated with ART use, missed HIV care appointments, and viral suppression for adolescents and young adults in the United States. Consideration of these factors is strongly recommended in future intervention, clinical practice, and policy research that seek to understand the contextual influences on individuals’ health behaviors.
Resource type:
Article
Affiliation Label Tesim:
Department of Medicine
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/wf1m-by29
Identifier:
PMCID: PMC4817971, Publisher DOI: https://doi.org/10.1371/journal.pone.0151106, Onescience id: cff7bddb6e14c85e68b6acfdb3b3773dca88ceef, and PMID: 27035905
ISSN:
1932-6203
Journal Issue:
4
Journal Title:
PloS One
Journal Volume:
11
Keyword:
HIV Infections, Adolescent, Hispanic Americans, United States, Female, African Continental Ancestry Group, HIV, Humans, Health Behavior, Young Adult, Male, Anti-Retroviral Agents, and Socioeconomic Factors
Language Label:
English
ORCID:
Other Affiliation:
Department of Preventive Medicine; Department of Public Health Program; College of Osteopathic Medicine; Nova Southeastern University, Department of Health Behavior and Health Education; School of Public Health; University of Michigan, Department of Health Sciences; DePaul University, and Division of Epidemiology; Services; and Prevention Research; National Institute on Drug Abuse; National Institutes of Health
Page Start:
e0151106
Person:
Fernandez, Maria I., Bauermeister, Jose A., Bruce, Douglas, Hightow-Weidman, Lisa B., Kahana, Shoshana Y., and Jenkins, Richard A.
Rights Statement Label:
In Copyright
Source:
kw52jd79k

1773. Strategies for Enriching Variant Coverage in Candidate Disease Loci on a Multiethnic Genotyping Array

Title Tesim:
Strategies for Enriching Variant Coverage in Candidate Disease Loci on a Multiethnic Genotyping Array
Creator:
Haiman, Christopher A., Haessler, Jeffrey, Buyske, Steven, Peters, Ulrike, Martin, Alicia R., Marchand, Loic Le, Carlson, Christopher S., Wojcik, Genevieve L., Martin, Lisa W., Franceschini, Nora, Loos, Ruth J. F., Hindorff, Lucia, James, Regina, Kocarnik, Jonathan M., Kooperberg, Charles L., Kenny, Eimear E., Zubair, Niha, Matise, Tara, Bustamante, Carlos D., Graff, Mariaelisa, Bien, Stephanie A., Walker, Ryan W., Xia, Lucy, Gignoux, Christopher R., North, Kari E., and Cheng, Iona
Date of publication:
2016
Abstract Tesim:
Investigating genetic architecture of complex traits in ancestrally diverse populations is imperative to understand the etiology of disease. However, the current paucity of genetic research in people of African and Latin American ancestry, Hispanic and indigenous peoples in the United States is likely to exacerbate existing health disparities for many common diseases. The Population Architecture using Genomics and Epidemiology, Phase II (PAGE II), Study was initiated in 2013 by the National Human Genome Research Institute to expand our understanding of complex trait loci in ethnically diverse and well characterized study populations. To meet this goal, the Multi-Ethnic Genotyping Array (MEGA) was designed to substantially improve fine-mapping and functional discovery by increasing variant coverage across multiple ethnicities at known loci for metabolic, cardiovascular, renal, inflammatory, anthropometric, and a variety of lifestyle traits. Studying the frequency distribution of clinically relevant mutations, putative risk alleles, and known functional variants across multiple populations will provide important insight into the genetic architecture of complex diseases and facilitate the discovery of novel, sometimes population-specific, disease associations. DNA samples from 51,650 self-identified African ancestry (17,328), Hispanic/Latino (22,379), Asian/Pacific Islander (8,640), and American Indian (653) and an additional 2,650 participants of either South Asian or European ancestry, and other reference panels have been genotyped on MEGA by PAGE II. MEGA was designed as a new resource for studying ancestrally diverse populations. Here, we describe the methodology for selecting trait-specific content for use in multi-ethnic populations and how enriching MEGA for this content may contribute to deeper biological understanding of the genetic etiology of complex disease.
Resource type:
Article
Affiliation Label Tesim:
Department of Epidemiology
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/p9sn-mc37
Identifier:
Publisher DOI: https://doi.org/10.1371/journal.pone.0167758, PMCID: PMC5156387, Onescience id: bb579486adbd0c6ab3ba3f9502803277e9af51ba, and PMID: 27973554
ISSN:
1932-6203
Journal Issue:
12
Journal Title:
PloS One
Journal Volume:
11
Keyword:
Genotype, United States, Ethnic Groups, Genome, Human, Genome-Wide Association Study, Mutation, Female, Chromosome Mapping, Alleles, Anthropometry, Humans, Male, Genetic Variation, Genomics, and Exome
Language Label:
English
ORCID:
Other Affiliation:
Department of Preventive Medicine; Keck School of Medicine; University of Southern California/Norris Comprehensive Cancer Center, Division of Public Health Sciences; Fred Hutchinson Cancer Research Center, Department of Genetics; School of Arts and Sciences; Rutgers University, Department of Epidemiology; University of Washington, Department of Genetics; Stanford University, Department of Epidemiology Program; University of Hawai'i Cancer Center, Division of Cardiology; George Washington University; School of Medicine and Health Sciences, Department of Preventive Medicine; Icahn School of Medicine at Mount Sinai, Charles Bronfman Institute for Personalized Medicine; Icahn School of Medicine at Mount Sinai, Division of Genomic Medicine; National Human Genome Research Institute; National Institutes of Health, Division of Intramural Research; National Institute of Child Health and Human Development; National Institutes of Health, and Cancer Prevention Institute of California
Page Start:
e0167758
Person:
Haiman, Christopher A., Haessler, Jeffrey, Buyske, Steven, Peters, Ulrike, Martin, Alicia R., Marchand, Loic Le, Carlson, Christopher S., Wojcik, Genevieve L., Martin, Lisa W., Franceschini, Nora, Loos, Ruth J. F., Hindorff, Lucia, James, Regina, Kocarnik, Jonathan M., Kooperberg, Charles L., Kenny, Eimear E., Zubair, Niha, Matise, Tara, Bustamante, Carlos D., Graff, Mariaelisa, Bien, Stephanie A., Walker, Ryan W., Xia, Lucy, Gignoux, Christopher R., North, Kari E., and Cheng, Iona
Rights Statement Label:
In Copyright
Source:
2z10ww017

1774. Stage-Specific MicroRNAs and Their Role in the Anticancer Effects of Calorie Restriction in a Rat Model of ER-Positive Luminal Breast Cancer

Title Tesim:
Stage-Specific MicroRNAs and Their Role in the Anticancer Effects of Calorie Restriction in a Rat Model of ER-Positive Luminal Breast Cancer
Creator:
Sanford, Tiffany, Lebourgeois, Paul, Harrison, Lauren M., Devlin, Kaylyn L., Mambo, Elizabeth, Lashinger, Laura M., and Hursting, Stephen D.
Date of publication:
2016
Abstract Tesim:
MicroRNAs have emerged as ubiquitous post-transcriptional regulators that coordinate many fundamental processes within cells, including those commonly linked to cancer when dysregulated. Profiling microRNAs across stages of cancer progression provides focus as to which microRNAs are key players in cancer development and are therefore important to manipulate with interventions to delay cancer onset and progression. Calorie restriction is one of the most effective preventive interventions across many types of cancer, although its effects on microRNAs have not been well characterized. We used the dimethylbenz[a]-anthracene-induced model of luminal mammary cancer in Sprague Dawley rats to elucidate which microRNAs are linked to progression in this type of cancer and, subsequently, to study how calorie restriction affects such microRNAs. We identified eight microRNAs (miR-10a, miR-10b, miR-21, miR-124, miR-125b, miR-126, miR-145 and miR-200a) to be associated with DMBA-induced mammary tumor progression. Calorie restriction, which greatly increased tumor-free survival and decreased the overall size of tumors that did develop, significantly decreased the expression of one microRNA, miR-200a, which was positively associated with tumor progression. We further showed that inhibition of miR-200a function, mimicking the effect of calorie restriction on this microRNA, inhibited proliferation in both rat (LA7) and human (MCF7) luminal mammary cancer cell lines. These findings present, for the first time, a stage-specific profile of microRNAs in a rodent model of luminal mammary cancer. Furthermore, we have identified the regulation of miR-200a, a microRNA that is positively associated with progression in this model, as a possible mechanism contributing to the anticancer effects of calorie restriction.
Resource type:
Article
Affiliation Label Tesim:
Department of Nutrition
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/dk3d-f718
Identifier:
Onescience id: 6d76dbfdc39c9ded2faf2fa55812aef1e39a00dc, PMCID: PMC4951048, Publisher DOI: https://doi.org/10.1371/journal.pone.0159686, and PMID: 27433802
ISSN:
1932-6203
Journal Issue:
7
Journal Title:
PloS One
Journal Volume:
11
Keyword:
Mir200a, Mir145, Neoplasms, MIR126, MIR21, MicroRNAs, Cell Line, Mir126, MIR145, Mir21, Mir10b, Homo sapiens, Rattus norvegicus, MIR10B, MIR200A, Cells, Anthracenes, Phenobarbital, Mir10a, MIR10A, and Breast Neoplasms
Language Label:
English
ORCID:
Other Affiliation:
Asuragen Incorporated, Luminex Corporation, Live Edge LLC, Department of Nutritional Sciences; University of Texas at Austin, Department of Molecular Biosciences; University of Texas at Austin, and Molecular Health
Page Start:
e0159686
Person:
Sanford, Tiffany, Lebourgeois, Paul, Harrison, Lauren M., Devlin, Kaylyn L., Mambo, Elizabeth, Lashinger, Laura M., and Hursting, Stephen D.
Rights Statement Label:
In Copyright
Source:
pz50h242n

1775. Staff Nurses’ Perceptions and Experiences about Structural Empowerment: A Qualitative Phenomenological Study

Title Tesim:
Staff Nurses’ Perceptions and Experiences about Structural Empowerment: A Qualitative Phenomenological Study
Creator:
Havens, Donna Sullivan, Van Rompaey, Bart, Van Bogaert, Peter, Van Heusden, Danny, Diltour, Nadine, Peremans, Lieve, and Dilles, Tinne
Date of publication:
2016
Abstract Tesim:
The aim of the study reported in this article was to investigate staff nurses’ perceptions and experiences about structural empowerment and perceptions regarding the extent to which structural empowerment supports safe quality patient care. To address the complex needs of patients, staff nurse involvement in clinical and organizational decision-making processes within interdisciplinary care settings is crucial. A qualitative study was conducted using individual semi-structured interviews of 11 staff nurses assigned to medical or surgical units in a 600-bed university hospital in Belgium. During the study period, the hospital was going through an organizational transformation process to move from a classic hierarchical and departmental organizational structure to one that was flat and interdisciplinary. Staff nurses reported experiencing structural empowerment and they were willing to be involved in decision-making processes primarily about patient care within the context of their practice unit. However, participants were not always fully aware of the challenges and the effect of empowerment on their daily practice, the quality of care and patient safety. Ongoing hospital change initiatives supported staff nurses’ involvement in decision-making processes for certain matters but for some decisions, a classic hierarchical and departmental process still remained. Nurses perceived relatively high work demands and at times viewed empowerment as presenting additional. Staff nurses recognized the opportunities structural empowerment provided within their daily practice. Nurse managers and unit climate were seen as crucial for success while lack of time and perceived work demands were viewed as barriers to empowerment.
Resource type:
Article
Affiliation Label Tesim:
University of North Carolina at Chapel Hill
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/kph8-xe07
Identifier:
PMCID: PMC4818078, Publisher DOI: https://doi.org/10.1371/journal.pone.0152654, Onescience id: 57b35f9da84dfdcecd6bce12a2d541e5179159a0, and PMID: 27035457
ISSN:
1932-6203
Journal Issue:
4
Journal Title:
PloS One
Journal Volume:
11
Keyword:
Decision Making, Nursing Staff, Population Groupings, Nursing Science, Hospital, Labor Economics, Medicine, Hospitals, Allied Health Care Professionals, Health Care Providers, Physicians, Economics, Professions, Political Science, Research Article, Science, People and Places, Cognition, Middle Aged, Employment, Social Sciences, Cognitive Science, Health Care, Nurses, Humans, R, Neuroscience, Medicine and Health Sciences, Medical Doctors, Jobs, Labor Studies, Power (Psychology), Biology and Life Sciences, Adult, Quality of Care, and Q
Language Label:
English
ORCID:
Other Affiliation:
Nursing and Midwifery Sciences; Centre for Research and Innovation in Care; Faculty of Medicine and Health Sciences; University of Antwerp, Nursing Department; Antwerp University Hospital, Department of Primary and Interdisciplinary Care; University of Antwerp, and Mental Health and Wellbeing Research Group; Vrije Universiteit Brussel
Page Start:
e0152654
Person:
Havens, Donna Sullivan, Van Rompaey, Bart, Van Bogaert, Peter, Van Heusden, Danny, Diltour, Nadine, Peremans, Lieve, and Dilles, Tinne
Rights Statement Label:
In Copyright
Source:
n009w731t

1776. Spatial Disassociation of Disrupted Functional Connectivity for the Default Mode Network in Patients with End-Stage Renal Disease

Title Tesim:
Spatial Disassociation of Disrupted Functional Connectivity for the Default Mode Network in Patients with End-Stage Renal Disease
Creator:
Ma, Xiaofen, Xu, Yikai, Dong, Jianwei, Zong, Xiaopeng, Li, Zibo, Tian, Junzhang, Jiang, Guihua, Zhan, Wenfeng, and Wu, Zhanhong
Date of publication:
2016
Abstract Tesim:
To investigate the aberrant functional connectivity of the default mode network (DMN) in patients with end-stage renal disease (ESRD) and their clinical relevance.
Resource type:
Article
Affiliation Label Tesim:
Department of Radiology
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/exwa-p738
Identifier:
PMID: 27560146, Publisher DOI: https://doi.org/10.1371/journal.pone.0161392, PMCID: PMC4999135, and Onescience id: f22c58900b669d2ffe877d95c7c0f497c1ffe706
ISSN:
1932-6203
Journal Issue:
8
Journal Title:
PloS One
Journal Volume:
11
Keyword:
Magnetic Resonance Imaging, Image Processing, Computer-Assisted, Kidney Failure, Chronic, Brain, Adult, Neural Pathways, Case-Control Studies, Rest, Humans, Female, Middle Aged, Male, Gyrus Cinguli, Frontal Lobe, and Brain Mapping
Language Label:
English
ORCID:
Other Affiliation:
Department of Medical Imaging; Guangdong Provincial No.2 People's Hospital, Department of Medical Imaging Center; Nanfang Hospital; Southern Medical University, and Department of Mathematics; Guangdong Pharmaceutical University
Page Start:
e0161392
Person:
Ma, Xiaofen, Xu, Yikai, Dong, Jianwei, Zong, Xiaopeng, Li, Zibo, Tian, Junzhang, Jiang, Guihua, Zhan, Wenfeng, and Wu, Zhanhong
Rights Statement Label:
In Copyright
Source:
q811kq78p

1777. Spaceflight Activates Lipotoxic Pathways in Mouse Liver

Title Tesim:
Spaceflight Activates Lipotoxic Pathways in Mouse Liver
Creator:
Bateman, Ted A., Stodieck, Louis S., Ferguson, Virginia L., Orlicky, David J., Gridley, Daila S., Pecaut, Michael J., Levi, Moshe, Alfonso-Garcia, Alba, Jonscher, Karen R., Bouxsein, Mary L., Potma, Eric O., Suhalim, Jeffrey L., and Friedman, Jacob E.
Date of publication:
2016
Abstract Tesim:
Spaceflight affects numerous organ systems in the body, leading to metabolic dysfunction that may have long-term consequences. Microgravity-induced alterations in liver metabolism, particularly with respect to lipids, remain largely unexplored. Here we utilize a novel systems biology approach, combining metabolomics and transcriptomics with advanced Raman microscopy, to investigate altered hepatic lipid metabolism in mice following short duration spaceflight. Mice flown aboard Space Transportation System -135, the last Shuttle mission, lose weight but redistribute lipids, particularly to the liver. Intriguingly, spaceflight mice lose retinol from lipid droplets. Both mRNA and metabolite changes suggest the retinol loss is linked to activation of PPARα-mediated pathways and potentially to hepatic stellate cell activation, both of which may be coincident with increased bile acids and early signs of liver injury. Although the 13-day flight duration is too short for frank fibrosis to develop, the retinol loss plus changes in markers of extracellular matrix remodeling raise the concern that longer duration exposure to the space environment may result in progressive liver damage, increasing the risk for nonalcoholic fatty liver disease.
Resource type:
Article
Affiliation Label Tesim:
UNC/NCSU Joint Department of Biomedical Engineering
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/gpdr-we90
Identifier:
Onescience id: 606b01e9a97f36ed3d6a90619f87c474be0668a2, Publisher DOI: https://doi.org/10.1371/journal.pone.0152877, PMCID: PMC4838331, and PMID: 27097220
ISSN:
1932-6203
Journal Issue:
4
Journal Title:
PloS One
Journal Volume:
11
Keyword:
Liver Diseases, Biology and Life Sciences, Fatty Liver, Vitamins, Genetics, Astronomical Sciences, Metabolism, Spaceflight, Biochemistry, Physiology, Anatomy, Chemistry, Gene Expression, Chemical compounds, Metabolic Pathways, Fatty Acids, Lipids, Space Exploration, Body Fluids, Organic compounds, Bile, Physical Sciences, Gastroenterology and Hepatology, Physical sciences, Medicine and Health Sciences, and Vitamin A
Language Label:
English
ORCID:
Other Affiliation:
BioServe Space Technologies; University of Colorado at Boulder, Department of Mechanical Engineering; University of Colorado at Boulder, Department of Pathology; University of Colorado Anschutz Medical Campus, Department of Basic Sciences; Division of Radiation Research; Loma Linda University School of Medicine, Department of Medicine; University of Colorado Anschutz Medical Campus, Department of Biomedical Engineering; University of California Irvine, Department of Anesthesiology; University of Colorado Anschutz Medical Campus, Department of Orthopedic Surgery; Harvard Medical School, Beckman Laser Institute and Medical Clinic; University of California Irvine, and Department of Pediatrics; University of Colorado Anschutz Medical Campus
Page Start:
e0152877
Person:
Bateman, Ted A., Stodieck, Louis S., Ferguson, Virginia L., Orlicky, David J., Gridley, Daila S., Pecaut, Michael J., Levi, Moshe, Alfonso-Garcia, Alba, Jonscher, Karen R., Bouxsein, Mary L., Potma, Eric O., Suhalim, Jeffrey L., and Friedman, Jacob E.
Rights Statement Label:
In Copyright
Source:
sf2689769

1778. Single-Step Fabrication of Computationally Designed Microneedles by Continuous Liquid Interface Production

Title Tesim:
Single-Step Fabrication of Computationally Designed Microneedles by Continuous Liquid Interface Production
Creator:
Moga, Katherine A., Caudill, Cassie L., Luft, J. Christopher, Johnson, Ashley R., Bloomquist, Cameron J., Shirvanyants, David, Mecham, Sue J., Tumbleston, John R., Ermoshkin, Alexander, and De Simone, Joseph M.
Date of publication:
2016
Abstract Tesim:
Microneedles, arrays of micron-sized needles that painlessly puncture the skin, enable transdermal delivery of medications that are difficult to deliver using more traditional routes. Many important design parameters, such as microneedle size, shape, spacing, and composition, are known to influence efficacy, but are notoriously difficult to alter due to the complex nature of microfabrication techniques. Herein, we utilize a novel additive manufacturing (“3D printing”) technique called Continuous Liquid Interface Production (CLIP) to rapidly prototype sharp microneedles with tuneable geometries (size, shape, aspect ratio, spacing). This technology allows for mold-independent, one-step manufacturing of microneedle arrays of virtually any design in less than 10 minutes per patch. Square pyramidal CLIP microneedles composed of trimethylolpropane triacrylate, polyacrylic acid and photopolymerizable derivatives of polyethylene glycol and polycaprolactone were fabricated to demonstrate the range of materials that can be utilized within this platform for encapsulating and controlling the release of therapeutics. These CLIP microneedles effectively pierced murine skin ex vivo and released the fluorescent drug surrogate rhodamine.
Resource type:
Article
Affiliation Label Tesim:
Department of Chemistry, Division of Molecular Pharmaceutics, Joint Department of Biomedical Engineering, and University of North Carolina at Chapel Hill
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/73kn-3y48
Identifier:
PMID: 27607247, PMCID: PMC5015976, Publisher DOI: https://doi.org/10.1371/journal.pone.0162518, and Onescience id: a0c215a158c532a2eeaf7db1bf2188f13268b8f4
ISSN:
1932-6203
Journal Issue:
9
Journal Title:
PloS One
Journal Volume:
11
Keyword:
Fluorescent Dyes, Permeability, Microinjections, Mice, Nude, trimethylolpropane triacrylate, Acrylates, Animals, Skin Absorption, and Drug Delivery Systems
Language Label:
English
ORCID:
Other Affiliation:
Carbon, Division of Molecular Pharmaceutics, University of North Carolina at Chapel Hill, Department of Chemistry, and Joint Department of Biomedical Engineering
Page Start:
e0162518
Person:
Moga, Katherine A., Caudill, Cassie L., Luft, J. Christopher, Johnson, Ashley R., Bloomquist, Cameron J., Shirvanyants, David, Mecham, Sue J., Tumbleston, John R., Ermoshkin, Alexander, and De Simone, Joseph M.
Rights Statement Label:
In Copyright
Source:
2227mv75q

1779. Single Synonymous Mutations in KRAS Cause Transformed Phenotypes in NIH3T3 Cells

Title Tesim:
Single Synonymous Mutations in KRAS Cause Transformed Phenotypes in NIH3T3 Cells
Creator:
Hartley, James L., Waters, Andrew M., Bagni, Rachel, and Portugal, Franklin
Date of publication:
2016
Abstract Tesim:
Synonymous mutations in the KRAS gene are clustered at G12, G13, and G60 in human cancers. We constructed 9 stable NIH3T3 cell lines expressing KRAS, each with one of these synonymous mutations. Compared to the negative control cell line expressing the wild type human KRAS gene, all the synonymous mutant lines expressed more KRAS protein, grew more rapidly and to higher densities, and were more invasive in multiple assays. Three of the cell lines showed dramatic loss of contact inhibition, were more refractile under phase contrast, and their refractility was greatly reduced by treatment with trametinib. Codon usage at these glycines is highly conserved in KRAS compared to HRAS, indicating selective pressure. These transformed phenotypes suggest that synonymous mutations found in driver genes such as KRAS may play a role in human cancers.
Resource type:
Article
Affiliation Label Tesim:
University of North Carolina at Chapel Hill
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/3q93-s212
Identifier:
PMCID: PMC5042562, Publisher DOI: https://doi.org/10.1371/journal.pone.0163272, PMID: 27684555, and Onescience id: f5d409aa2fb4d30928fc4c9f1952c755d2505c80
ISSN:
1932-6203
Journal Issue:
9
Journal Title:
PloS One
Journal Volume:
11
Keyword:
MAPK signaling cascades, Glycine, Mutation, Protein expression, Missense mutation, Hyperexpression techniques, Transformed cell lines, and Extracellular matrix
Language Label:
English
ORCID:
Other Affiliation:
Cancer Research Technology Program; Frederick National Laboratory for Cancer Research; Leidos Biomedical Research; Inc. and Biology Department; Catholic University of America
Page Start:
e0163272
Person:
Hartley, James L., Waters, Andrew M., Bagni, Rachel, and Portugal, Franklin
Rights Statement Label:
In Copyright
Source:
6682x950f

1780. Simulating Flying Insects Using Dynamics and Data-Driven Noise Modeling to Generate Diverse Collective Behaviors

Title Tesim:
Simulating Flying Insects Using Dynamics and Data-Driven Noise Modeling to Generate Diverse Collective Behaviors
Creator:
Manocha, Dinesh, Wang, Xinjie, Ren, Jiaping, and Jin, Xiaogang
Date of publication:
2016
Abstract Tesim:
We present a biologically plausible dynamics model to simulate swarms of flying insects. Our formulation, which is based on biological conclusions and experimental observations, is designed to simulate large insect swarms of varying densities. We use a force-based model that captures different interactions between the insects and the environment and computes collision-free trajectories for each individual insect. Furthermore, we model the noise as a constructive force at the collective level and present a technique to generate noise-induced insect movements in a large swarm that are similar to those observed in real-world trajectories. We use a data-driven formulation that is based on pre-recorded insect trajectories. We also present a novel evaluation metric and a statistical validation approach that takes into account various characteristics of insect motions. In practice, the combination of Curl noise function with our dynamics model is used to generate realistic swarm simulations and emergent behaviors. We highlight its performance for simulating large flying swarms of midges, fruit fly, locusts and moths and demonstrate many collective behaviors, including aggregation, migration, phase transition, and escape responses.
Resource type:
Article
Affiliation Label Tesim:
Department of Computer Science
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/w2km-sw91
Identifier:
Onescience id: e07b53c19f0b27a095d595a4e8665a405180878e, PMCID: PMC4871504, PMID: 27187068, and Publisher DOI: https://doi.org/10.1371/journal.pone.0155698
ISSN:
1932-6203
Journal Issue:
5
Journal Title:
PloS One
Journal Volume:
11
Keyword:
Models, Biological, Drosophila, Behavior, Animal, Flight, Animal, Female, Computer Simulation, Insecta, Grasshoppers, Male, and Animals
Language Label:
English
ORCID:
Other Affiliation:
State Key Lab. of CAD and CG; Zhejiang University
Page Start:
e0155698
Person:
Manocha, Dinesh, Wang, Xinjie, Ren, Jiaping, and Jin, Xiaogang
Rights Statement Label:
In Copyright
Source:
pc289q04r