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UNC-authored articles published by the Public Library of Science (PLoS)

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1891. Comparison of the adolescent and adult mouse prefrontal cortex proteome

Title Tesim:
Comparison of the adolescent and adult mouse prefrontal cortex proteome
Creator:
Agoglia, Abigail E., Spanos, Marina, Holstein, Sarah E., Small, Amanda T., Hodge, Clyde W., and Burrus, Brainard M.
Date of publication:
2017
Abstract Tesim:
Adolescence is a developmental period characterized by unique behavioral phenotypes (increased novelty seeking, risk taking, sociability and impulsivity) and increased risk for destructive behaviors, impaired decision making and psychiatric illness. Adaptive and maladaptive adolescent traits have been associated with development of the medial prefrontal cortex (mPFC), a brain region that mediates regulatory control of behavior. However, the molecular changes that underlie brain development and behavioral vulnerability have not been fully characterized. Using high-throughput 2D DIGE spot profiling with identification by MALDI-TOF mass spectrometry, we identified 62 spots in the PFC that exhibited age-dependent differences in expression. Identified proteins were associated with diverse cellular functions, including intracellular signaling, synaptic plasticity, cellular organization and metabolism. Separate Western blot analyses confirmed age-related changes in DPYSL2, DNM1, STXBP1 and CFL1 in the mPFC and expanded these findings to the dorsal striatum, nucleus accumbens, motor cortex, amygdala and ventral tegmental area. Ingenuity Pathway Analysis (IPA) identified functional interaction networks enriched with proteins identified in the proteomics screen, linking age-related alterations in protein expression to cellular assembly and development, cell signaling and behavior, and psychiatric illness. These results provide insight into potential molecular components of adolescent cortical development, implicating structural processes that begin during embryonic development as well as plastic adaptations in signaling that may work in concert to bring the cortex, and other brain regions, into maturity.
Resource type:
Article
Affiliation Label Tesim:
School of Medicine
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/tbag-zb39
Identifier:
PMID: 28570644, Onescience id: c0eb529b3ed80ff696288a4346e56013d69fe969, Publisher DOI: https://doi.org/10.1371/journal.pone.0178391, and PMCID: PMC5453624
ISSN:
1932-6203
Journal Issue:
6
Journal Title:
PloS One
Journal Volume:
12
Keyword:
Nerve Tissue Proteins, Electrophoresis, Gel, Two-Dimensional, Mass Spectrometry, Mice, Inbred C57BL, Prefrontal Cortex, Mice, Animals, Proteome, and Male
Language Label:
English
ORCID:
Other Affiliation:
Neuroscience Curriculum and Department of Psychiatry
Page Start:
e0178391
Person:
Agoglia, Abigail E., Spanos, Marina, Holstein, Sarah E., Small, Amanda T., Hodge, Clyde W., and Burrus, Brainard M.
Rights Statement Label:
In Copyright
Source:
c247dz097

1892. Community burden and prognostic impact of reduced kidney function among patients hospitalized with acute decompensated heart failure: The Atherosclerosis Risk in Communities (ARIC) Study Community Surveillance

Title Tesim:
Community burden and prognostic impact of reduced kidney function among patients hospitalized with acute decompensated heart failure: The Atherosclerosis Risk in Communities (ARIC) Study Community Surveillance
Creator:
Coresh, Josef, Wruck, Lisa M., Chang, Patricia P., Rosamond, Wayne, Hyun, Noorie, Matsushita, Kunihiro, Kwak, Lucia, Agarwal, Sunil K., Loehr, Laura R., Bessel, Marina, and Ni, Hanyu
Date of publication:
2017
Abstract Tesim:
Kidney dysfunction is prevalent and impacts prognosis in patients with acute decompensated heart failure (ADHF). However, most previous reports were from a single hospital, limiting their generalizability. Also, contemporary data using new equation for estimated glomerular filtration rate (eGFR) are needed.
Resource type:
Article
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/9z0h-5g95
Identifier:
Onescience id: 1adab922a71dfdd4d47243ec5d0b836c93ba758c, PMID: 28793319, Publisher DOI: https://doi.org/10.1371/journal.pone.0181373, and PMCID: PMC5549913
ISSN:
1932-6203
Journal Issue:
8
Journal Title:
PloS One
Journal Volume:
12
Keyword:
Male, Risk Assessment, Kidney Function Tests, Public Health Surveillance, Blood Urea Nitrogen, Creatinine, Kidney, Atherosclerosis, Cost of Illness, Kidney Diseases, Prognosis, Glomerular Filtration Rate, Aged, Humans, Heart Failure, and Female
Language Label:
English
ORCID:
and 0000-0002-7179-718X
Other Affiliation:
Duke Clinical Research Institute; United States of America
Page Start:
e0181373
Person:
Coresh, Josef, Wruck, Lisa M., Chang, Patricia P., Rosamond, Wayne, Hyun, Noorie, Matsushita, Kunihiro, Kwak, Lucia, Agarwal, Sunil K., Loehr, Laura R., Bessel, Marina, and Ni, Hanyu
Rights Statement Label:
In Copyright
Source:
j098zh29g

1893. Characteristics of HIV-Infected Children at Enrollment into Care and at Antiretroviral Therapy Initiation in Central Africa

Title Tesim:
Characteristics of HIV-Infected Children at Enrollment into Care and at Antiretroviral Therapy Initiation in Central Africa
Creator:
Edmonds, Andrew, Anastos, Kathryn, Sinayobye, Jean D'Amour, Nduwimana, Martin, Adedimeji, Adebola, Lelo, Patricia, Yotebieng, Marcel, Shi, Qiuhu, Hoover, Donald, and Nash, Denis
Date of publication:
2017
Abstract Tesim:
Despite the World Health Organization (WHO) regularly updating guidelines to recommend earlier initiation of antiretroviral therapy (ART) in children, timely enrollment into care and initiation of ART in sub-Saharan Africa in children lags behind that of adults. The impact of implementing increasingly less restrictive ART guidelines on ART initiation in Central Africa has not been described.
Resource type:
Article
Affiliation Label Tesim:
Department of Epidemiology
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/tysj-5578
Identifier:
PMCID: PMC5230784, Onescience id: 6b0505caabb209058571f0eddc803c7fa7d56c31, PMID: 28081230, and Publisher DOI: https://doi.org/10.1371/journal.pone.0169871
ISSN:
1932-6203
Journal Issue:
1
Journal Title:
PloS One
Journal Volume:
12
Keyword:
Practice Guidelines as Topic, Rwanda, Humans, Child, Retrospective Studies, Adolescent, Infant, Newborn, Anti-Retroviral Agents, HIV-1, Infant, Burundi, Child, Preschool, Africa, Central, HIV Infections, Delivery of Health Care, Male, and Female
Language Label:
English
ORCID:
Other Affiliation:
Department of Epidemiology and Population Health; Albert Einstein College of Medicine, Department of Medicine; Montefiore Medical Center, Division of Research and Clinical Education; Rwanda Military Hospital, Department of Pediatrics; University Hospital of Kamenge; Faculty of Medicine; University of Burundi, Kalembe Lembe Pediatric Hospital, College of Public Health; Division of Epidemiology; Ohio State University, Department of Epidemiology and Community Health; New York Medical College, Department of Statistics; Rutgers University, and Epidemiology and Biostatistics Program; City University of New York; School of Public Health
Page Start:
e0169871
Person:
Edmonds, Andrew, Anastos, Kathryn, Sinayobye, Jean D'Amour, Nduwimana, Martin, Adedimeji, Adebola, Lelo, Patricia, Yotebieng, Marcel, Shi, Qiuhu, Hoover, Donald, and Nash, Denis
Rights Statement Label:
In Copyright
Source:
9p290g34v

1894. Characteristics and outcomes of older HIV-infected patients receiving antiretroviral therapy in Malawi: A retrospective observation cohort study

Title Tesim:
Characteristics and outcomes of older HIV-infected patients receiving antiretroviral therapy in Malawi: A retrospective observation cohort study
Creator:
Phiri, Sam, Makwinja, Agness, Feldacker, Caryl, Heller, Tom, Gugsa, Salem, Tweya, Hannock, Kalulu, Mike, Chiwoko, Jane, Ng'ambi, Wingston, Banda, Rabecca, and Nthala, Omisher
Date of publication:
2017
Abstract Tesim:
To estimate patients enrolling on antiretroviral therapy (ART) over time; describe trends in baseline characteristics; and compare immunological response, loss to follow-up (LTFU), and mortality by three age groups (25–39, 40–49 and ≥50 years).
Resource type:
Article
Affiliation Label Tesim:
Department of Medicine
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/sbms-2380
Identifier:
PMID: 28686636, Onescience id: 74fd766c11d443a7993fd261466715bf8b6478fb, Publisher DOI: https://doi.org/10.1371/journal.pone.0180232, and PMCID: PMC5501463
ISSN:
1932-6203
Journal Issue:
7
Journal Title:
PloS One
Journal Volume:
12
Keyword:
Age Factors, Survival Analysis, CD4 Lymphocyte Count, Treatment Outcome, Anti-HIV Agents, Malawi, Retrospective Studies, Immunity, Innate, Female, Middle Aged, Adult, Male, Humans, CD4-Positive T-Lymphocytes, HIV Infections, and Lost to Follow-Up
Language Label:
English
ORCID:
Other Affiliation:
Lighthouse Trust, International Training and Education Center for Health; University of Washington, and International Union Against Tuberculosis and Lung Disease
Page Start:
e0180232
Person:
Phiri, Sam, Makwinja, Agness, Feldacker, Caryl, Heller, Tom, Gugsa, Salem, Tweya, Hannock, Kalulu, Mike, Chiwoko, Jane, Ng'ambi, Wingston, Banda, Rabecca, and Nthala, Omisher
Rights Statement Label:
In Copyright
Source:
rv043079q

1895. Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice

Title Tesim:
Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice
Creator:
Forsberg, Lawrence J., Sexton, Jonathan Z., Renner, Sarah W., Walker, Lauren M., and Brenman, Jay E.
Date of publication:
2017
Abstract Tesim:
Carbonic anhydrases are a family of enzymes that catalyze the reversible condensation of water and carbon dioxide to carbonic acid, which spontaneously dissociates to bicarbonate. Carbonic anhydrase III (Car3) is nutritionally regulated at both the mRNA and protein level. It is highly enriched in tissues that synthesize and/or store fat: liver, white adipose tissue, brown adipose tissue, and skeletal muscle. Previous characterization of Car3 knockout mice focused on mice fed standard diets, not high-fat diets that significantly alter the tissues that highly express Car3. We observed lower protein levels of Car3 in high-fat diet fed mice treated with niclosamide, a drug published to improve fatty liver symptoms in mice. However, it is unknown if Car3 is simply a biomarker reflecting lipid accumulation or whether it has a functional role in regulating lipid metabolism. We focused our in vitro studies toward metabolic pathways that require bicarbonate. To further determine the role of Car3 in metabolism, we measured de novo fatty acid synthesis with in vitro radiolabeled experiments and examined metabolic biomarkers in Car3 knockout and wild type mice fed high-fat diet. Specifically, we analyzed body weight, body composition, metabolic rate, insulin resistance, serum and tissue triglycerides. Our results indicate that Car3 is not required for de novo lipogenesis, and Car3 knockout mice fed high-fat diet do not have significant differences in responses to various diets to wild type mice.
Resource type:
Article
Affiliation Label Tesim:
School of Medicine and Curriculum in Genetics and Molecular Biology
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/2hr0-2a05
Identifier:
PMID: 28437447, PMCID: PMC5402959, Onescience id: bb39c7358bb99ad6cec15e71049820af5b849626, and Publisher DOI: https://doi.org/10.1371/journal.pone.0176502
ISSN:
1932-6203
Journal Issue:
4
Journal Title:
PloS One
Journal Volume:
12
Keyword:
Body Composition, Male, Diet, High-Fat, Fatty Acids, Mice, Knockout, Carbonic Anhydrase III, Liver, Animals, Adipose Tissue, White, Body Weight, Insulin Resistance, Lipogenesis, Adipose Tissue, Brown, Lipid Metabolism, and Mice
Language Label:
English
ORCID:
Other Affiliation:
Biomanufacturing Research Institute and Technology Enterprise; North Carolina Central University, School of Medicine, and Department of Cell Biology and Physiology
Page Start:
e0176502
Person:
Forsberg, Lawrence J., Sexton, Jonathan Z., Renner, Sarah W., Walker, Lauren M., and Brenman, Jay E.
Rights Statement Label:
In Copyright
Source:
9p290g33k

1896. Can a simulation-based training program impact the use of evidence based routine practices at birth? Results of a hospital-based cluster randomized trial in Mexico

Title Tesim:
Can a simulation-based training program impact the use of evidence based routine practices at birth? Results of a hospital-based cluster randomized trial in Mexico
Creator:
Angeles, Gustavo, Lamadrid-Figueroa, Hector, Cohen, Susanna, Walker, Dilys M., and Fritz, Jimena
Date of publication:
2017
Abstract Tesim:
In Mexico, although the majority of births are attended in hospitals, reports have emerged of obstetric violence, use of unsafe practices, and failure to employ evidence-based practices (EBP). Recent attention has refocused global efforts towards provision of quality care that is both patient-centered and evidence-based. Scaling up of local interventions should rely on strong evidence of effectiveness.
Resource type:
Article
Affiliation Label Tesim:
Department of Maternal and Child Health
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/jrb3-5n18
Identifier:
PMID: 28319122, Publisher DOI: https://doi.org/10.1371/journal.pone.0172623, Onescience id: 9a07a23a46c134c89b68b7b7e3f935a8b5706993, and PMCID: PMC5358738
ISSN:
1932-6203
Journal Issue:
3
Journal Title:
PloS One
Journal Volume:
12
Keyword:
Mexico, Hospitals, Autonomic Fibers, Preganglionic, Delivery, Obstetric, Evidence-Based Practice, Quality Improvement, Patient Care Team, Humans, Intensive Care, Neonatal, Emergency Medical Services, and Health Personnel
Language Label:
English
ORCID:
Other Affiliation:
Division of Reproductive Health; Research Center for Population Health; National Institute of Public Health (INSP), College of Nursing; University of Utah, and Department of Obstetrics; Gynecology and Reproductive Sciences; Bixby Center for Global Reproductive Health; University of California in San Francisco (UCSF)
Page Start:
e0172623
Person:
Angeles, Gustavo, Lamadrid-Figueroa, Hector, Cohen, Susanna, Walker, Dilys M., and Fritz, Jimena
Rights Statement Label:
In Copyright
Source:
p8418t357

1897. BRL3 and AtRGS1 cooperate to fine tune growth inhibition and ROS activation

Title Tesim:
BRL3 and AtRGS1 cooperate to fine tune growth inhibition and ROS activation
Creator:
Tunc-Ozdemir, Meral and Jones, Alan M.
Date of publication:
2017
Abstract Tesim:
Plasma membrane-localized leucine-rich repeat receptor-like kinases directly activates G protein complex via interaction with seven transmembrane domain Regulator of G-protein Signaling 1 (AtRGS1) protein. Brassinosteroid insensitive 1 (BRI1) LIKE3 (BRL3) phosphorylates AtRGS1 in vitro. FRET analysis showed that BRL3 and AtRGS1 interaction dynamics change in response to glucose and flg22. Both BRL3 and AtRGS1 function in glucose sensing and brl3 and rgs1-2 single mutants are hyposensitive to high glucose as well as the brl3/rgs1 double mutant. BRL3 and AtRGS1 function in the same pathway linked to high glucose sensing. Hypocotyl elongation, another sugar-mediated pathway, is also implicated to be partially mediated by BRL3 and AtRGS1 because rgs1-2, brl3-2 and brl3-2/ rgs1-2 mutants share the long hypocotyl phenotype. BRL3 and AtRGS1 modulate the flg22-induced ROS burst and block one or more components positively regulating ROS production because the brl3/rgs1 double mutant has ~60% more ROS production than wild type while rgs1-2 has a partial ROS burst impairment and brl3 has slightly more ROS production. Here, we proposed a simple model where both BRL3 and AtRGS1 are part of a fine-tuning mechanism sensing glucose and flg22 to prevent excess ROS burst and control growth inhibition.
Resource type:
Article
Affiliation Label Tesim:
Department of Biology
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/xfjh-xv11
Identifier:
PMCID: PMC5436702, Onescience id: 32934dc6a60587290880e52475f8062870490e59, PMID: 28545052, and Publisher DOI: https://doi.org/10.1371/journal.pone.0177400
ISSN:
1932-6203
Journal Issue:
5
Journal Title:
PloS One
Journal Volume:
12
Keyword:
RGS Proteins, BRL3 protein, Arabidopsis, Mutation, Arabidopsis, Reactive Oxygen Species, Receptors, Cell Surface, Peptides, Glucose, and Arabidopsis Proteins
Language Label:
English
ORCID:
Other Affiliation:
Department of Pharmacology
Page Start:
e0177400
Person:
Tunc-Ozdemir, Meral and Jones, Alan M.
Rights Statement Label:
In Copyright
Source:
hd76s490v

1898. BIRC6 mediates imatinib resistance independently of Mcl-1

Title Tesim:
BIRC6 mediates imatinib resistance independently of Mcl-1
Creator:
Graves, Lee M., Zhang, Raymond, Zhang, Yanping, Herring, Laura E., Gilbert, Thomas S. K., East, Michael P., Okumu, Denis O., Levine, Merlin, and Litchfield, David W.
Date of publication:
2017
Abstract Tesim:
Baculoviral IAP repeat containing 6 (BIRC6) is a member of the inhibitors of apoptosis proteins (IAPs), a family of functionally and structurally related proteins that inhibit apoptosis. BIRC6 has been implicated in drug resistance in several different human cancers, however mechanisms regulating BIRC6 have not been extensively explored. Our phosphoproteomic analysis of an imatinib-resistant chronic myelogenous leukemia (CML) cell line (MYL-R) identified increased amounts of a BIRC6 peptide phosphorylated at S480, S482, and S486 compared to imatinib-sensitive CML cells (MYL). Thus we investigated the role of BIRC6 in mediating imatinib resistance and compared it to the well-characterized anti-apoptotic protein, Mcl-1. Both BIRC6 and Mcl-1 were elevated in MYL-R compared to MYL cells. Lentiviral shRNA knockdown of BIRC6 in MYL-R cells increased imatinib-stimulated caspase activation and resulted in a ~20-25-fold increase in imatinib sensitivity, without affecting Mcl-1. Treating MYL-R cells with CDK9 inhibitors decreased BIRC6 mRNA, but not BIRC6 protein levels. By contrast, while CDK9 inhibitors reduced Mcl-1 mRNA and protein, they did not affect imatinib sensitivity. Since the Src family kinase Lyn is highly expressed and active in MYL-R cells, we tested the effects of Lyn inhibition on BIRC6 and Mcl-1. RNAi-mediated knockdown or inhibition of Lyn (dasatinib/ponatinib) reduced BIRC6 protein stability and increased caspase activation. Inhibition of Lyn also increased formation of an N-terminal BIRC6 fragment in parallel with reduced amount of the BIRC6 phosphopeptide, suggesting that Lyn may regulate BIRC6 phosphorylation and stability. In summary, our data show that BIRC6 stability is dependent on Lyn, and that BIRC6 mediates imatinib sensitivity independently of Mcl-1 or CDK9. Hence, BIRC6 may be a novel target for the treatment of drug-resistant CML where Mcl-1 or CDK9 inhibitors have failed.
Resource type:
Article
Affiliation Label Tesim:
Department of Pharmacology and Department of Chemistry
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/qjbq-b671
Identifier:
PMCID: PMC5433768, Publisher DOI: https://doi.org/10.1371/journal.pone.0177871, Onescience id: 968099adf57541bed16f1d73508b6e555ecdd608, and PMID: 28520795
ISSN:
1932-6203
Journal Issue:
5
Journal Title:
PloS One
Journal Volume:
12
Keyword:
Humans, Imatinib Mesylate, Cell Line, Tumor, src-Family Kinases, Cyclin-Dependent Kinase 9, Drug Resistance, Neoplasm, Inhibitor of Apoptosis Proteins, BIRC6 protein, human, Antineoplastic Agents, Myeloid Cell Leukemia Sequence 1 Protein, and Apoptosis
Language Label:
English
ORCID:
Other Affiliation:
University of North Carolina at Chapel Hill, Department of Radiation Oncology, and Department of Biochemistry; Schulich School of Medicine and Dentistry; Western University
Page Start:
e0177871
Person:
Graves, Lee M., Zhang, Raymond, Zhang, Yanping, Herring, Laura E., Gilbert, Thomas S. K., East, Michael P., Okumu, Denis O., Levine, Merlin, and Litchfield, David W.
Rights Statement Label:
In Copyright
Source:
76537668p

1899. Awareness and trust of the FDA and CDC: Results from a national sample of US adults and adolescents

Title Tesim:
Awareness and trust of the FDA and CDC: Results from a national sample of US adults and adolescents
Creator:
Hannan, Anika, Schmidt, Allison M., Kowitt, Sarah D., and Goldstein, Adam O.
Date of publication:
2017
Abstract Tesim:
Trust in government agencies plays a key role in advancing these organizations' agendas, influencing behaviors, and effectively implementing policies. However, few studies have examined the extent to which individuals are aware of and trust the leading United States agencies devoted to protecting the public’s health. Using two national samples of adolescents (N = 1,125) and adults (N = 5,014), we examined demographic factors, with a focus on vulnerable groups, as correlates of awareness of and trust in the Centers for Disease Control and Prevention (CDC), Food and Drug Administration (FDA), and the federal government. From nine different weighted and adjusted logistic regression models, we found high levels of awareness of the existence of the FDA and CDC (ranging from 55.7% for adolescents’ awareness of the CDC to 94.3% for adults’ awareness of the FDA) and moderate levels of trust (ranging from a low of 41.8% for adults’ trust in the federal government and a high of 78.8% for adolescents’ trust of the FDA). In the adolescent and adult samples, awareness was higher among non-Hispanic Blacks and respondents with low numeracy. With respect to trust, few consistent demographic differences emerged. Our findings provide novel insights regarding awareness and trust in the federal government and specific United States public health agencies. Our findings suggest groups to whom these agencies may want to selectively communicate to enhance trust and thus facilitate their communication and regulatory agendas.
Resource type:
Article
Affiliation Label Tesim:
University of North Carolina at Chapel Hill and Department of Health Behavior
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/1s15-et95
Identifier:
Publisher DOI: https://doi.org/10.1371/journal.pone.0177546, PMID: 28520750, Onescience id: 3ce48a298528caa000ba63b8c3ccb6bc18c27eeb, and PMCID: PMC5433718
ISSN:
1932-6203
Journal Issue:
5
Journal Title:
PloS One
Journal Volume:
12
Keyword:
Male, Federal Government, Adolescent, United States, Young Adult, United States Food and Drug Administration, Trust, Middle Aged, Centers for Disease Control and Prevention (U.S.), Humans, Surveys and Questionnaires, Female, Adult, Awareness, and Public Opinion
Language Label:
English
ORCID:
Other Affiliation:
Department of Family Medicine
Page Start:
e0177546
Person:
Hannan, Anika, Schmidt, Allison M., Kowitt, Sarah D., and Goldstein, Adam O.
Rights Statement Label:
In Copyright
Source:
jd4733040

1900. Association of HIV diversity and virologic outcomes in early antiretroviral treatment: HPTN 052

Title Tesim:
Association of HIV diversity and virologic outcomes in early antiretroviral treatment: HPTN 052
Creator:
Eshleman, Susan H., Kumwenda, Newton, McCauley, Marybeth, Gamble, Theresa, Kumarasamy, Nagalingeswaran, Godbole, Sheela V., Makhema, Joseph, Palumbo, Philip J., Hosseinipour, Mina C., Melo, Marineide G., Hakim, James G., Pilotto, Jose H., Grinsztejn, Beatriz, Chariyalertsak, Suwat, Chen, Ying Q., Cohen, Myron S., Panchia, Ravindre, Wilson, Ethan A., Fogel, Jessica M., and Piwowar-Manning, Estelle
Date of publication:
2017
Abstract Tesim:
Higher HIV diversity has been associated with virologic outcomes in children on antiretroviral treatment (ART). We examined the association of HIV diversity with virologic outcomes in adults from the HPTN 052 trial who initiated ART at CD4 cell counts of 350–550 cells/mm3. A high resolution melting (HRM) assay was used to analyze baseline (pre-treatment) HIV diversity in six regions in the HIV genome (two in gag, one in pol, and three in env) from 95 participants who failed ART. We analyzed the association of HIV diversity in each genomic region with baseline (pre-treatment) factors and three clinical outcomes: time to virologic suppression after ART initiation, time to ART failure, and emergence of HIV drug resistance at ART failure. After correcting for multiple comparisons, we did not find any association of baseline HIV diversity with demographic, laboratory, or clinical characteristics. For the 18 analyses performed for clinical outcomes evaluated, there was only one significant association: higher baseline HIV diversity in one of the three HIV env regions was associated with longer time to ART failure (p = 0.008). The HRM diversity assay may be useful in future studies exploring the relationship between HIV diversity and clinical outcomes in individuals with HIV infection.
Resource type:
Article
Affiliation Label Tesim:
Division of Infectious Diseases and Department of Medicine
Deposit Record:
http://windsor.libint.unc.edu:8181/fcrepo/rest/prod/e2/82/bb/5c/e282bb5c-ecfc-4dd2-8603-2a46b5cc8e4b
Type:
http://purl.org/dc/dcmitype/Text
DOI:
https://doi.org/10.17615/5s2y-pb56
Identifier:
Onescience id: f2b24122d653bcca54103f9da48897b86ca85289, Publisher DOI: https://doi.org/10.1371/journal.pone.0177281, PMCID: PMC5421787, and PMID: 28481902
ISSN:
1932-6203
Journal Issue:
5
Journal Title:
PloS One
Journal Volume:
12
Keyword:
Child, HIV, Viral Load, CD4 Lymphocyte Count, Anti-HIV Agents, Treatment Outcome, Male, Cohort Studies, HIV Infections, Humans, and Female
Language Label:
English
ORCID:
Other Affiliation:
Dept. of Pathology; Johns Hopkins Univ.; School of Medicine, College of Medicine-Johns Hopkins Project, Science Facilitation Department; FHI 360, CART CRS; YRGCARE Medical Centre; VHS, National AIDS Research Institute; ICMR, Botswana-Harvard AIDS Institute Partnership, UNC Project-Malawi; Institute for Global Health and Infectious Diseases, Hospital Nossa Senhora da Conceicaõ; Servico de Infectologia, Dept. of Medicine; Univ. of Zimbabwe, Hospital Geral de Nova Iguacu; Laboratorio de AIDS e Imunologia Molecular-IOC/Fiocruz, Instituto Nacional de Infectologia Evandro Chagas-INI-Fiocruz, Research Institute for Health Sciences; Chiang Mai University, Vaccine and Infectious Disease Division; Fred Hutchinson Cancer Research Center, and Univ. of the Witwatersrand; Perinatal HIV Research Unit; Soweto HPTN CRS
Page Start:
e0177281
Person:
Eshleman, Susan H., Kumwenda, Newton, McCauley, Marybeth, Gamble, Theresa, Kumarasamy, Nagalingeswaran, Godbole, Sheela V., Makhema, Joseph, Palumbo, Philip J., Hosseinipour, Mina C., Melo, Marineide G., Hakim, James G., Pilotto, Jose H., Grinsztejn, Beatriz, Chariyalertsak, Suwat, Chen, Ying Q., Cohen, Myron S., Panchia, Ravindre, Wilson, Ethan A., Fogel, Jessica M., and Piwowar-Manning, Estelle
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