In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies
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Li, Dapeng, et al. In Vitro and In Vivo Functions of Sars-cov-2 Infection-enhancing and Neutralizing Antibodies. 2021. https://doi.org/10.17615/tj07-s761APA
Li, D., Edwards, R., Manne, K., Martinez, D., Schäfer, A., Alam1, S., Wiehe, K., Lu, X., Parks, R., Sutherland, L., Oguin Iii, T., Mc Danal, C., Perez, L., Mansouri, K., Gobeil, S., Janowska, K., Stalls, V., Kopp, M., Cai, F., Lee, E., Foulger, A., Hernandez, G., Sanzone, A., Tilahun, K., Jiang, C., Tse, L., Bock, K., Minai, M., Nagata, B., Cronin, K., Gee Lai, V., Deyton, M., Barr, M., Von Holle, T., Macintyre, A., Stover, E., Feldman, J., Hauser, B., Caradonna, T., Scobey, T., Rountree, W., Wang, Y., Moody, M., Cain, D., De Marco, C., Denny, T., Woods, C., Petzold, E., Schmidt, A., Teng, I., Zhou, T., Kwong, P., Mascola, J., Graham, B., Moore, I., Seder, R., Andersen, H., Lewis, M., Montefiori, D., Sempowski, G., Baric, R., Acharya1, P., Haynes, B., & Saunders, K. (2021). In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies. https://doi.org/10.17615/tj07-s761Chicago
Li, Dapeng, Robert J Edwards, Kartik Manne, David R. Martinez, Alexandra Schäfer, S. Munir Alam1, Kevin Wiehe et al. 2021. In Vitro and In Vivo Functions of Sars-Cov-2 Infection-Enhancing and Neutralizing Antibodies. https://doi.org/10.17615/tj07-s761- Creator
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Li, Dapeng
- Other Affiliation: Duke University School of Medicine
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Edwards, Robert J.
- Other Affiliation: Duke University School of Medicine
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Manne, Kartik
- Other Affiliation: Duke University School of Medicine
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Martinez, David R.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Schäfer, Alexandra
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Alam1, S. Munir
- Other Affiliation: Duke University School of Medicine
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Wiehe, Kevin
- Other Affiliation: Duke University School of Medicine
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Lu, Xiaozhi
- Other Affiliation: Duke University School of Medicine
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Parks, Robert
- Other Affiliation: Duke University School of Medicine
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Sutherland, Laura L.
- Other Affiliation: Duke University School of Medicine
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Oguin III, Thomas H.
- Other Affiliation: Duke University School of Medicine
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McDanal, Charlene
- Other Affiliation: National Institutes of Health
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Perez, Lautaro G.
- Other Affiliation: National Institutes of Health
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Mansouri, Katayoun
- Other Affiliation: Duke University School of Medicine
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Gobeil, Sophie M. C.
- Other Affiliation: Duke University School of Medicine
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Janowska, Katarzyna J.
- Other Affiliation: Duke University School of Medicine
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Stalls, Victoria
- Other Affiliation: Duke University School of Medicine
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Kopp, Megan
- Other Affiliation: Duke University School of Medicine
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Cai, Fangping
- Other Affiliation: Duke University School of Medicine
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Lee, Esther
- Other Affiliation: Duke University School of Medicine
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Foulger, Andrew
- Other Affiliation: Duke University School of Medicine
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Hernandez, Giovanna E.
- Other Affiliation: Duke University School of Medicine
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Sanzone, Aja
- Other Affiliation: Duke University School of Medicine
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Tilahun, Kedamawit
- Other Affiliation: Duke University School of Medicine
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Jiang, Chuancang
- Other Affiliation: Duke University School of Medicine
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Tse, Longping V.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Bock, Kevin W.
- Other Affiliation: National Institutes of Health
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Minai, Mahnaz
- Other Affiliation: National Institutes of Health
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Nagata, Bianca M.
- Other Affiliation: National Institutes of Health
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Cronin, Kenneth
- Other Affiliation: Duke University School of Medicine
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Gee-Lai, Victoria
- Other Affiliation: Duke University School of Medicine
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Deyton, Margaret
- Other Affiliation: Duke University School of Medicine
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Barr, Maggie
- Other Affiliation: Duke University School of Medicine
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Von Holle, Tarra
- Other Affiliation: Duke University School of Medicine
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Macintyre, Andrew N.
- Other Affiliation: Duke University School of Medicine
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Stover, Erica
- Other Affiliation: Duke University School of Medicine
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Feldman, Jared
- Other Affiliation: Ragon Institute of MGH, MIT and Harvard
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Hauser, Blake M.
- Other Affiliation: Ragon Institute of MGH, MIT and Harvard
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Caradonna, Timothy M.
- Other Affiliation: Ragon Institute of MGH, MIT and Harvard
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Scobey, Trevor D.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Rountree, Wes
- Other Affiliation: Duke University School of Medicine
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Wang, Yunfei
- Other Affiliation: Duke University School of Medicine
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Moody, M. Anthony
- Other Affiliation: Duke University School of Medicine
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Cain, Derek W.
- Other Affiliation: Duke University School of Medicine
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DeMarco, C. Todd
- Other Affiliation: Duke University School of Medicine
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Denny, Thomas N.
- Other Affiliation: Duke University School of Medicine
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Woods, Christopher W.
- Other Affiliation: Duke University School of Medicine
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Petzold, Elizabeth W.
- Other Affiliation: Duke University Medical Center
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Schmidt, Aaron G.
- Other Affiliation: Ragon Institute of MGH, MIT and Harvard
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Teng, I-Ting
- Other Affiliation: National Institutes of Health
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Zhou, Tongqing
- Other Affiliation: National Institutes of Health
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Kwong, Peter D.
- Other Affiliation: National Institutes of Health
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Mascola, John R.
- Other Affiliation: National Institutes of Health
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Graham, Barney S.
- Other Affiliation: National Institutes of Health
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Moore, Ian N.
- Other Affiliation: National Institutes of Health
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Seder, Robert
- Other Affiliation: National Institutes of Health
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Andersen, Hanne
- Other Affiliation: BIOQUAL
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Lewis, Mark G.
- Other Affiliation: BIOQUAL
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Montefiori, David C.
- Other Affiliation: Duke University
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Sempowski, Gregory D.
- Other Affiliation: Duke University School of Medicine
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Baric, Ralph S.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Acharya1, Priyamvada
- Other Affiliation: Duke University School of Medicine
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Haynes, Barton F.
- Other Affiliation: Duke University School of Medicine
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Saunders, Kevin O.
- Other Affiliation: Duke University School of Medicine
- Abstract
- SARS-CoV-2 neutralizing antibodies (NAbs) protect against COVID-19. A concern regarding SARS-CoV-2 antibodies is whether they mediate disease enhancement. Here, we isolated NAbs against the receptor-binding domain (RBD) and the N-terminal domain (NTD) of SARS-CoV-2 spike from individuals with acute or convalescent SARS-CoV-2 or a history of SARS-CoV infection. Cryo-electron microscopy of RBD and NTD antibodies demonstrated function-specific modes of binding. Select RBD NAbs also demonstrated Fc receptor-g (FcgR)-mediated enhancement of virus infection in vitro, while five non-neutralizing NTD antibodies mediated FcgR-independent in vitro infection enhancement. However, both types of infection-enhancing antibodies protected from SARS-CoV-2 replication in monkeys and mice. Three of 46 monkeys infused with enhancing antibodies had higher lung inflammation scores compared to controls. One monkey had alveolar edema and elevated bronchoalveolar lavage inflammatory cytokines. Thus, while in vitro antibody-enhanced infection does not necessarily herald enhanced infection in vivo, increased lung inflammation can rarely occur in SARS-CoV-2 antibody-infused macaques.
- Date of publication
- 2021
- Keyword
- DOI
- Identifier
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Cell
- Language
- English
- Version
- Postprint
- ISSN
- 0092-8674
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