Control of T lymphocyte morphology by the GTPase Rho Public Deposited

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Creator
  • Wooten, David K
    • Other Affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, 77030, USA
  • Teague, T Kent
    • Other Affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, 77030, USA; Department of Surgery, Oklahoma City Health Science Center, Oklahoma City, OK, USA
  • Woodside, Darren G
    • Other Affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, 77030, USA; Department of Immunology, Texas Biotechnology Corporation, Houston, TX, USA
  • Caudell, Eva G
    • Other Affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, 77030, USA
  • Udagawa, Taturo
    • Other Affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, 77030, USA; Children's Hospital, Department of Surgical Research, Enders-10, 300 Longwood Ave., Boston, MA 02115, USA
  • Andruss, Bernard F
    • Other Affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, 77030, USA
  • Miyamoto, Yuko J
    • Other Affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, 77030, USA
  • McIntyre, Bradley W
    • Other Affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, 77030, USA; Children's Hospital, Department of Surgical Research, Enders-10, 300 Longwood Ave., Boston, MA 02115, USA
Abstract
  • Abstract Background Rho family GTPase regulation of the actin cytoskeleton governs a variety of cell responses. In this report, we have analyzed the role of the GTPase Rho in maintenance of the T lymphocyte actin cytoskeleton. Results Inactivation of the GTPase Rho in the human T lymphocytic cell line HPB-ALL does not inhibit constitutively high adhesion to the integrin β1 substrate fibronectin. It did however result in the aberrant extension of finger-like dendritic processes on the substrates VCAM-1, Fn, and mAb specific to β1 integrins. Time-lapse video microscopy demonstrated that C3 induced extensions were primarily the result of an altered pseudopod elongation rather than retraction. Once the stellate pseudopodia extended, none retracted, and cells became completely immobile. Filipodial structures were absent and the dendritic-like processes in C3 treated cells were rich in filamentous actin. Immunolocalization of RhoA in untreated HPB-ALL cells spreading on fibronectin demonstrated a diffuse staining pattern within the pseudopodia. In C3 treated cells, clusters of RhoA were pronounced and localized within the altered extensions. Conclusions GTPase Rho is actively involved in the regulation of T lymphocyte morphology and motility.
Date of publication
Identifier
  • doi:10.1186/1471-2121-4-2
  • 12600279
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Darren G Woodside et al.; licensee BioMed Central Ltd.
Journal title
  • BMC Cell Biology
Journal volume
  • 4
Journal issue
  • 1
Page start
  • 2
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1471-2121
Bibliographic citation
  • BMC Cell Biology. 2003 Feb 24;4(1):2
Access
  • Open Access
Publisher
  • BioMed Central Ltd
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