Increased plasma mannose binding lectin levels are associated with bronchiolitis obliterans after lung transplantation Public Deposited

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Creator
  • Tilley, Stephen
    • Affiliation: School of Medicine, Department of Medicine, Division of Pulmonary Diseases and Critical Care Medicine
  • Aris, Robert M
    • Affiliation: School of Medicine, Department of Medicine, Division of Pulmonary Diseases and Critical Care Medicine
  • Medaiyese, Ayorinde A
    • Other Affiliation: Pikeville Medical Cen, Pikeville, KY, USA
  • Budd, Steven J
    • Other Affiliation: Protochips, Raleigh, NC, USA
  • Neuringer, Isabel P
    • Other Affiliation: Pulmonary and Critical Care Unit, Massachusetts General Hospital, 148 Bulfinch Bldg., 55 Fruit Street, Boston, MA 02114, USA
Abstract
  • Abstract Background Long-term lung allograft survival is limited by bronchiolitis obliterans syndrome (BOS). Mannose binding lectin (MBL) belongs to the innate immune system, participates in complement activation, and may predispose to graft rejection. We investigated mannose binding (MBL) during cold ischemia and in tissue samples from explanted lungs with BOS, and assessed MBL and complement proteins in plasma post-lung transplantation relative to BOS staging. Methods MBL was detected by immunohistochemistry lung tissue at the time of cold ischemia and in samples with BOS. MBL was assayed in the peripheral blood of 66 lung transplant patients transplanted between 1990–2007. Results MBL localized to vasculature and basement membrane during cold ischemia and BOS. Patients further out post-lung transplant > 5 years (n = 33), had significantly lower levels of MBL in the blood compared to lung transplant patients < 5 years with BOS Op-3 (n = 17), 1738 ± 250 ng/ml vs 3198 ± 370 ng/ml, p = 0.027, and similar levels to lung transplant patients < 5 years with BOS 0 (n = 16), 1738 ± 250 ng/ml vs 1808 ± 345 ng/ml. MBL levels in all BOS 0 (n = 30) vs. all BOS Op-3 (n = 36) were 1378 ± 275 ng/ml vs. 2578 ± 390 ng/ml, p = 0.001, respectively. C3 plasma levels in BOS 0 (n = 30) vs. BOS Op-3 (n = 36) were 101 ± 19.8 mg/ml vs. 114 ± 25.2 mg/ml, p = 0.024, respectively. Conclusions MBL localizes within the lung during graft ischemia and BOS, higher levels of plasma MBL are associated with BOS Op-3 and < 5 years post-transplant, and higher level of plasma complement protein C3 was associated with BOS Op-3 clinical status. MBL may serve as a biomarker for poorer outcome post-lung transplantation.
Date of publication
Identifier
  • 22762710
  • doi:10.1186/1465-9921-13-56
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Steven J Budd et al.; licensee BioMed Central Ltd.
License
Journal title
  • Respiratory Research
Journal volume
  • 13
Journal issue
  • 1
Page start
  • 56
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1465-9921
Bibliographic citation
  • Respiratory Research. 2012 Jul 04;13(1):56
Access
  • Open Access
Publisher
  • BioMed Central Ltd
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