Characterization of an immediate-early gene induced in adherent monocytes that encodes IκB-like activity Public Deposited

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Creator
  • Baldwin, Albert
    • ORCID: https://orcid.org/0000-0003-1246-1347
    • Affiliation: College of Arts and Sciences, Department of Biology, N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center, School of Medicine, Curriculum in Genetics and Molecular Biology
  • Sampson-Johannes, Adam
    • Other Affiliation: Department of Cell Biology, Cetus Corporation, Emeryville, California 94808
  • Ralph, Peter
    • Other Affiliation: Department of Cell Biology, Cetus Corporation, Emeryville, California 94808
  • Yurochko, Andrew D.
    • Affiliation: N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center, School of Medicine
  • Tompkins, S. Mark
    • Affiliation: N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center, School of Medicine
  • Haskill, Stephen
    • Affiliation: School of Medicine, Department of Microbiology and Immunology, N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center, Department of Obstetrics and Gynecology
  • Morris, John
    • Affiliation: N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center, School of Medicine
  • Berg, Amer A.
    • Affiliation: College of Arts and Sciences, Department of Biology, N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center, School of Medicine
  • Mondal, Krishna
    • Affiliation: School of Medicine, Department of Microbiology and Immunology
Abstract
  • We have cloned a group of cDNAs representing mRNAs that are rapidly induced following adherence of human monocytes. One of the induced transcripts (MAD-3) encodes a protein of 317 amino acids with one domain containing five tandem repeats of the cdc10/ankyrin motif, which is 60% similar (46% identical) to the ankyrin repeat region of the precursor of NF-κBKBF1 p50. The C-terminus has a putative protein kinase C phosphorylation site. In vitro translated MAD-3 protein was found to specifically inhibit the DNA-binding activity of the p50p65 NF-κB complex but not that of the p50p50 KBF1 factor or of other DNA-binding proteins. The MAD-3 cDNA encodes an IκB-like protein that is likely to be involved in regulation of transcriptional responses to NF-κB, including adhesion-dependent pathways of monocyte activation.
Date of publication
Identifier
  • 2-s2.0-0025812292
  • doi:10.1016/0092-8674(91)90022-Q
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Resource type
  • Article
Rights statement
  • In Copyright
Journal title
  • Cell
Journal volume
  • 65
Journal issue
  • 7
Page start
  • 1281
Page end
  • 1289
Language
  • English
Version
  • Postprint
ISSN
  • 0092-8674
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