Differentially charged isoforms of apolipoprotein E from human blood are potential biomarkers of Alzheimer’s disease Public Deposited

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Creator
  • DeKroon, Robert M
    • Affiliation: School of Medicine
  • Osorio, Cristina
    • Affiliation: School of Medicine
  • Gunawardena, Harsha P
    • Affiliation: School of Medicine, Department of Biochemistry and Biophysics
  • Corcimaru, Ana
    • Affiliation: School of Medicine
  • Alzate, Oscar
    • Affiliation: School of Medicine
    • Other Affiliation: School of Medicine, Universidad Pontificia Bolivariana, Medellin, Colombia; Current address: 108 Reynolds Medical Building, College of Medicine, Texas A&M Health Science Center, College Station, TX 77843-1114, USA
Abstract
  • Abstract Introduction Alzheimer’s disease (AD) is the major cause of dementia among the elderly. Finding blood-based biomarkers for disease diagnosis and prognosis is urgently needed. Methods We studied protein distributions in brain tissues, cerebrospinal fluid (CSF), and blood of AD patients by using proteomics and a new proteomic method that we call “2D multiplexed Western blot” (2D mxWd). This method allows us to determine in multiple samples the electrophoretic patterns of protein isoforms with different isoelectric points. Results Apolipoprotein E (ApoE) displays a unique distribution of electrophoretic isoforms in the presence of AD and also a unique pattern specific to the APOE genotype. Conclusions The isoelectric distribution of differentially charged ApoE isoforms was used to determine the presence of AD in a small group of samples. Further studies are needed to validate their use as predictors of disease onset and progression, and as biomarkers for determining the efficacy of therapeutic treatments.
Date of publication
Identifier
  • doi:10.1186/alzrt273
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Oscar Alzate et al.; licensee BioMed Central Ltd.
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Journal title
  • Alzheimer's Research & Therapy
Journal volume
  • 6
Journal issue
  • 4
Page start
  • 43
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1758-9193
Bibliographic citation
  • Alzheimer's Research & Therapy. 2014 Jul 14;6(4):43
Access
  • Open Access
Publisher
  • BioMed Central Ltd
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