Systemic Dietary Hesperidin Modulation of Osteoclastogenesis, Bone Homeostasis and Periodontal Disease in Mice
Public Deposited- Creator
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de Paiva Gonçalves, Vinícius
- Affiliation: School of Dentistry
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Musskopf, Marta Liliana
- Affiliation: School of Dentistry
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Rivera-Concepcion, Angeliz
- Affiliation: School of Dentistry
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Yu, Christina
- Affiliation: School of Dentistry
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Wong, Sing Wai
- Affiliation: School of Dentistry
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Tuin, Stephen A.
- Affiliation: School of Dentistry
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Jiao, Yizu
- Affiliation: School of Dentistry
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Susin, Cristiano
- Affiliation: School of Dentistry
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Spolidorio, Luís Carlos
- Other Affiliation: Araraquara School of Dentistry
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Miguez, Patricia Almeida
- Affiliation: School of Dentistry
- Abstract
- This study aimed to evaluate the effects of hesperidin (HE) on in vitro osteoclastogenesis and dietary supplem entation on mouse periodontal disease and femoral bone phenotype. RAW 264.7 cells were stimulated with RANKL in the presence or absence of HE (1, 100 or 500 µM) for 5 days, and evaluated by TRAP, TUNEL and Western Blot (WB) analyses. In vivo, C57BL/6 mice were given HE via oral gavage (125, 250 and 500 mg/kg) for 4 weeks. A sterile silk ligature was placed between the first and second right maxillary molars for 10 days and microcomputed tomography (μCT), histopathological and immunohistochemical evaluation were performed. Femoral bones subjected or not to dietary HE (500 mg/kg) for 6 and 12 weeks were evaluated using μCT. In vitro, HE 500 µM reduced formation of RANKL-stimulated TRAP-positive(+) multinucleated cells (500 µM) as well as c-Fos and NFATc1 protein expression (p < 0.05), markers of osteoclasts. In vivo, dietary HE 500 mg/kg increased the alveolar bone resorption in ligated teeth (p < 0.05) and resulted in a significant increase in TRAP+ cells (p < 0.05). Gingival inflammatory infiltrate was greater in the HE 500 mg/kg group even in the absence of ligature. In femurs, HE 500 mg/kg protected trabecular and cortical bone mass at 6 weeks of treatment. In conclusion, HE impaired in vitro osteoclastogenesis, but on the contrary, oral administration of a high concentration of dietary HE increased osteoclast numbers and promoted inflammation-induced alveolar bone loss. However, HE at 500 mg/kg can promote a bone-sparing effect on skeletal bone under physiological conditions.
- Date of publication
- 2022
- Keyword
- DOI
- Identifier
- Resource type
- Article
- Rights statement
- In Copyright
- License
- Attribution 3.0 United States
- Journal title
- International Journal of Molecular Sciences
- Journal volume
- 23
- Journal issue
- 13
- Page start
- 7100
- Language
- English
- Version
- Publisher
- ISSN
- 1422-0067
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This work has no parents.
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