A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence
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D Menachery, Vineet, et al. A Sars-like Cluster of Circulating Bat Coronaviruses Shows Potential for Human Emergence. Nature America, Inc., 2015. https://doi.org/10.17615/kx4a-mx62APA
D Menachery, V., L Yount Jr, B., Debbink, K., Agnihothram, S., E Gralinski, L., A Plante, J., L Graham, R., Scobey, T., Yi Ge, X., F Donaldson, E., H Randell, S., Lanzavecchia, A., A Marasco, W., Li Shi, Z., & S Baric, R. (2015). A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. Nature America, Inc. https://doi.org/10.17615/kx4a-mx62Chicago
D Menachery, Vineet, Boyd L Yount Jr, Kari Debbink, Sudhakar Agnihothram, Lisa E Gralinski, Jessica A Plante, Rachel L Graham et al. 2015. A Sars-Like Cluster of Circulating Bat Coronaviruses Shows Potential for Human Emergence. Nature America, Inc.. https://doi.org/10.17615/kx4a-mx62- Creator
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Vineet D Menachery
- Other Affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Boyd L Yount Jr
- Other Affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Kari Debbink
- Other Affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA ; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Sudhakar Agnihothram
- Other Affiliation: National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas, USA
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Lisa E Gralinski
- Other Affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Jessica A Plante
- Other Affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Rachel L Graham
- Other Affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Trevor Scobey
- Other Affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Xing-Yi Ge
- Other Affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
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Eric F Donaldson
- Other Affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Scott H Randell
- Other Affiliation: Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA ; Cystic Fibrosis Center, Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Antonio Lanzavecchia
- Other Affiliation: Institute for Research in Biomedicine, Bellinzona Institute of Microbiology, Zurich, Switzerland
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Wayne A Marasco
- Other Affiliation: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
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Zhengli-Li Shi
- Other Affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
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Ralph S Baric
- Other Affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA ; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
- Abstract
- The emergence of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Middle East Respiratory Syndrome (MERS)-CoV underscores the threat of cross-species transmission events leading to outbreaks in humans. In this study, we examine the disease potential for SARS-like CoVs currently circulating in Chinese horseshoe bat populations. Utilizing the SARS-CoV infectious clone, we generated and characterized a chimeric virus expressing the spike of bat coronavirus SHC014 in a mouse adapted SARS-CoV backbone. The results indicate that group 2b viruses encoding the SHC014 spike in a wild type backbone can efficiently utilize multiple ACE2 receptor orthologs, replicate efficiently in primary human airway cells, and achieve in vitro titers equivalent to epidemic strains of SARS-CoV. Additionally, in vivo experiments demonstrate replication of the chimeric virus in mouse lung with notable pathogenesis. Evaluation of available SARS-based immune-therapeutic and prophylactic modalities revealed poor efficacy; both monoclonal antibody and vaccine approaches failed to neutralize and protect from CoVs utilizing the novel spike protein. Importantly, based on these findings, we synthetically rederived an infectious full length SHC014 recombinant virus and demonstrate robust viral replication both in vitro and in vivo. Together, the work highlights a continued risk of SARS-CoV reemergence from viruses currently circulating in bat populations.
- Date of publication
- 2015
- Keyword
- Subject
- DOI
- Related resource URL
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Nature Medicine
- Journal volume
- 21
- Journal issue
- 12
- Page start
- 1508
- Page end
- 1513
- Language
- English
- ISSN
- 1546-170X
- 1078-8956
- Publisher
- Nature America, Inc.
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