DNA vaccine protection against SARS-CoV-2 in rhesus macaques
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Yu, J, et al. Dna Vaccine Protection Against Sars-cov-2 In Rhesus Macaques. American Association for the Advancement of Science, 2020. https://doi.org/10.17615/7ywj-4d04APA
Yu, J., Tostanoski, L., Peter, L., Mercado, N., Mc Mahan, K., Mahrokhian, S., Nkolola, J., Liu, J., Li, Z., Chandrashekar, A., Martinez, D., Loos, C., Atyeo, C., Fischinger, S., Burke, J., Slein, M., Chen, Y., Zuiani, A., Lelis, F., Travers, M., Habibi, S., Pessaint, L., Van Ry, A., Blade, K., Brown, R., Cook, A., Finneyfrock, B., Dodson, A., Teow, E., Velasco, J., Zahn, R., Wegmann, F., Bondzie, E., Dagotto, G., Gebre, M., He, X., Jacob Dolan, C., Kirilova, M., Kordana, N., Lin, Z., Maxfield, L., Nampanya, F., Nityanandam, R., Ventura, J., Wan, H., Cai, Y., Chen, B., Schmidt, A., Wesemann, D., Baric, R., Alter, G., Andersen, H., Lewis, M., & Barouch, D. (2020). DNA vaccine protection against SARS-CoV-2 in rhesus macaques. American Association for the Advancement of Science. https://doi.org/10.17615/7ywj-4d04Chicago
Yu, J., L.H Tostanoski, L Peter, N.B Mercado, K Mc Mahan, S.H Mahrokhian, J.P Nkolola et al. 2020. Dna Vaccine Protection Against Sars-Cov-2 In Rhesus Macaques. American Association for the Advancement of Science. https://doi.org/10.17615/7ywj-4d04- Creator
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Yu, J.
- Other Affiliation: Harvard Medical School
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Tostanoski, L.H.
- Other Affiliation: Harvard Medical School
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Peter, L.
- Other Affiliation: Harvard Medical School
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Mercado, N.B.
- Other Affiliation: Harvard Medical School
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McMahan, K.
- Other Affiliation: Harvard Medical School
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Mahrokhian, S.H.
- Other Affiliation: Harvard Medical School
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Nkolola, J.P.
- Other Affiliation: Harvard Medical School
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Liu, J.
- Other Affiliation: Harvard Medical School
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Li, Z.
- Other Affiliation: Harvard Medical School
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Chandrashekar, A.
- Other Affiliation: Harvard Medical School
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Martinez, D.R.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Loos, C.
- Other Affiliation: Ragon Institute of MGH, MIT, and Harvard
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Atyeo, C.
- Other Affiliation: Ragon Institute of MGH, MIT, and Harvard
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Fischinger, S.
- Other Affiliation: Ragon Institute of MGH, MIT, and Harvard
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Burke, J.S.
- Other Affiliation: Ragon Institute of MGH, MIT, and Harvard
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Slein, M.D.
- Other Affiliation: Ragon Institute of MGH, MIT, and Harvard
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Chen, Y.
- Other Affiliation: Harvard Medical School
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Zuiani, A.
- Other Affiliation: Harvard Medical School
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Lelis, F.J.N.
- Other Affiliation: Harvard Medical School
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Travers, M.
- Other Affiliation: Harvard Medical School
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Habibi, S.
- Other Affiliation: Harvard Medical School
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Pessaint, L.
- Other Affiliation: Bioqual
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Van Ry, A.
- Other Affiliation: Bioqual
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Blade, K.
- Other Affiliation: Bioqual
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Brown, R.
- Other Affiliation: Bioqual
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Cook, A.
- Other Affiliation: Bioqual
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Finneyfrock, B.
- Other Affiliation: Bioqual
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Dodson, A.
- Other Affiliation: Bioqual
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Teow, E.
- Other Affiliation: Bioqual
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Velasco, J.
- Other Affiliation: Bioqual
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Zahn, R.
- Other Affiliation: Janssen Vaccines & Prevention BV
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Wegmann, F.
- Other Affiliation: Janssen Vaccines & Prevention BV
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Bondzie, E.A.
- Other Affiliation: Harvard Medical School
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Dagotto, G.
- Other Affiliation: Harvard Medical School
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Gebre, M.S.
- Other Affiliation: Harvard Medical School
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He, X.
- Other Affiliation: Harvard Medical School
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Jacob-Dolan, C.
- Other Affiliation: Harvard Medical School
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Kirilova, M.
- Other Affiliation: Harvard Medical School
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Kordana, N.
- Other Affiliation: Harvard Medical School
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Lin, Z.
- Other Affiliation: Harvard Medical School
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Maxfield, L.F.
- Other Affiliation: Harvard Medical School
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Nampanya, F.
- Other Affiliation: Harvard Medical School
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Nityanandam, R.
- Other Affiliation: Harvard Medical School
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Ventura, J.D.
- Other Affiliation: Harvard Medical School
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Wan, H.
- Other Affiliation: Harvard Medical School
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Cai, Y.
- Other Affiliation: Children's Hospital, Boston
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Chen, B.
- Other Affiliation: Children's Hospital, Boston
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Schmidt, A.G.
- Other Affiliation: Ragon Institute of MGH, MIT, and Harvard
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Wesemann, D.R.
- Other Affiliation: Harvard Medical School
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Baric, R.S.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Alter, G.
- Other Affiliation: Ragon Institute of MGH, MIT, and Harvard
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Andersen, H.
- Other Affiliation: Bioqual
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Lewis, M.G.
- Other Affiliation: Bioqual
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Barouch, D.H.
- Other Affiliation: Harvard Medical School
- Abstract
- The global coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made the development of a vaccine a top biomedical priority. In this study, we developed a series of DNA vaccine candidates expressing different forms of the SARS-CoV-2 spike (S) protein and evaluated them in 35 rhesus macaques. Vaccinated animals developed humoral and cellular immune responses, including neutralizing antibody titers at levels comparable to those found in convalescent humans and macaques infected with SARS-CoV-2. After vaccination, all animals were challenged with SARS-CoV-2, and the vaccine encoding the full-length S protein resulted in >3.1 and >3.7 log10 reductions in median viral loads in bronchoalveolar lavage and nasal mucosa, respectively, as compared with viral loads in sham controls. Vaccine-elicited neutralizing antibody titers correlated with protective efficacy, suggesting an immune correlate of protection. These data demonstrate vaccine protection against SARS-CoV-2 in nonhuman primates.
- Date of publication
- 2020
- Keyword
- Macaca mulatta
- Viral Vaccines
- Adjuvants, Immunologic
- Nasal Mucosa
- Vaccines, DNA
- Vaccination
- Betacoronavirus
- Immunization, Secondary
- Immunity, Cellular
- Primates
- Viral Load
- Mutant Proteins
- Animalia
- SARS coronavirus
- Disease Models, Animal
- Humans
- Male
- Antibodies, Viral
- Animals
- Pandemics
- Female
- Bronchoalveolar Lavage Fluid
- Coronavirus
- Immunity, Humoral
- Spike Glycoprotein, Coronavirus
- Immunogenicity, Vaccine
- Macaca
- Pneumonia, Viral
- Immunologic Memory
- Antibodies, Neutralizing
- Coronavirus Infections
- Protein Domains
- DOI
- Identifier
- https://dx.doi.org/10.1126/science.abc6284
- PMID 32434945
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Science
- Journal volume
- 369
- Journal issue
- 6505
- Page start
- 806
- Page end
- 811
- Language
- English
- ISSN
- 0036-8075
- Publisher
- American Association for the Advancement of Science
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