Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium

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  • Vorn, Rany
    • Other Affiliation: Johns Hopkins University
  • Mithani, Sara
    • Other Affiliation: National Institutes of Health
  • Devoto, Christina
    • Other Affiliation: National Institutes of Health
  • Meier, Timothy B.
    • Other Affiliation: Medical College of Wisconsin
  • Lai, Chen
    • Other Affiliation: National Institutes of Health
  • Yun, Sijung
    • Other Affiliation: Predictiv Care
  • Broglio, Steven P.
    • Other Affiliation: University of Michigan
  • McAllister, Thomas W.
    • Other Affiliation: Indiana University School of Medicine
  • Giza, Christopher C.
    • Other Affiliation: University of California, Los Angeles
  • Kim, Hyung-Suk
    • Other Affiliation: National Institutes of Health
  • Huber, Daniel
    • Other Affiliation: Medical College of Wisconsin
  • Harezlak, Jaroslaw
    • Other Affiliation: Indiana University, Bloomington
  • Cameron, Kenneth L.
    • Other Affiliation: Keller Army Hospital
  • McGinty, Gerald
    • Other Affiliation: United States Air Force Academy
  • Jackson, Jonathan
    • Other Affiliation: United States Air Force Academy
  • Guskiewicz, Kevin M.
    • Affiliation: College of Arts and Sciences, Department of Exercise and Sport Science
  • Mihalik, Jason P.
    • Affiliation: College of Arts and Sciences, Department of Exercise and Sport Science
  • Brooks, Alison
    • Other Affiliation: University of Wisconsin-Madison
  • Duma, Stefan
    • Other Affiliation: Virginia Tech
  • Rowson, Steven
    • Other Affiliation: Virginia Tech
  • Nelson, Lindsay D.
    • Other Affiliation: Medical College of Wisconsin
  • Pasquina, Paul
    • Other Affiliation: Uniformed Services University of the Health Sciences
  • McCrea, Michael A.
    • Other Affiliation: Medical College of Wisconsin
  • Gill, Jessica M.
    • Other Affiliation: Johns Hopkins University
  • CARE Consortium Investigators
Abstract
  • ObjectiveTo investigate the plasma proteomic profiling in identifying biomarkers related to return to sport (RTS) following a sport-related concussion (SRC).MethodsThis multicenter, prospective, case-control study was part of a larger cohort study conducted by the NCAA-DoD Concussion Assessment, Research, and Education (CARE) Consortium, athletes (n = 140) with blood collected within 48 h of injury and reported day to asymptomatic were included in this study, divided into two groups: (1) recovery <14-days (n = 99) and (2) recovery ≥14-days (n = 41). We applied a highly multiplexed proteomic technique that uses DNA aptamers assay to target 1,305 proteins in plasma samples from concussed athletes with <14-days and ≥14-days.ResultsWe identified 87 plasma proteins significantly dysregulated (32 upregulated and 55 downregulated) in concussed athletes with recovery ≥14-days relative to recovery <14-days groups. The significantly dysregulated proteins were uploaded to Ingenuity Pathway Analysis (IPA) software for analysis. Pathway analysis showed that significantly dysregulated proteins were associated with STAT3 pathway, regulation of the epithelial mesenchymal transition by growth factors pathway, and acute phase response signaling.ConclusionOur data showed the feasibility of large-scale plasma proteomic profiling in concussed athletes with a <14-days and ≥ 14-days recovery. These findings provide a possible understanding of the pathophysiological mechanism in neurobiological recovery. Further study is required to determine whether these proteins can aid clinicians in RTS decisions.
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  • Article
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  • In Copyright
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  • Attribution 4.0 International
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  • Frontiers in Neurology
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  • 13
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  • English
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  • 1664-2295

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