MicroRNA circulating in the early aftermath of motor vehicle collision predict persistent pain development and suggest a role for microRNA in sex-specific pain differences

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  • Linnstaedt, Sarah
    • Affiliation: School of Medicine, Department of Anesthesiology
    • Other Affiliation: TRYUMPH Research Program, Chapel Hill, NC, USA
  • Walker, Margaret G
    • Affiliation: School of Medicine, Department of Anesthesiology
    • Other Affiliation: TRYUMPH Research Program, Chapel Hill, NC, USA
  • Parker, Joel
    • Affiliation: N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
  • Yeh, Eunice
    • Affiliation: School of Medicine, Department of Anesthesiology
    • Other Affiliation: TRYUMPH Research Program, Chapel Hill, NC, USA
  • Sons, Robert L
    • Affiliation: School of Medicine, Department of Cell Biology and Physiology
  • Zimny, Erin
    • Other Affiliation: Department of Emergency Medicine, Henry Ford Hospital, Detroit, MI, USA
  • Lewandowski, Christopher
    • Other Affiliation: Department of Emergency Medicine, Henry Ford Hospital, Detroit, MI, USA
  • Hendry, Phyllis L
    • Other Affiliation: Department of Emergency Medicine, University of Florida College of Medicine-Jacksonville, Gainesville, FL, USA
  • Damiron, Kathia
    • Other Affiliation: Department of Emergency Medicine, Albert Einstein Medical Center, Philadelphia, PA, USA
  • Pearson, Claire
    • Other Affiliation: Department of Emergency Medicine, Detroit Receiving, Detroit, MI, USA
  • Velilla, Marc-Anthony
    • Other Affiliation: Department of Emergency Medicine, Sinai Grace, Detroit, MI, USA
  • O’Neil, Brian J
    • Other Affiliation: Department of Emergency Medicine, Wayne State University, Detroit, MI, USA; The Cardiovascular Research Institute, School of Medicine, Wayne State University, Detroit, MI, USA
  • Jones, Jeffrey
    • Other Affiliation: Department of Emergency Medicine, Spectrum Health Butterworth Campus, Grand Rapids, MI, USA
  • Swor, Robert
    • Other Affiliation: Department of Emergency Medicine, William Beaumont Hospital, Troy, MI, USA
  • Domeier, Robert
    • Other Affiliation: Department of Emergency Medicine, St Joseph Mercy Health System, Ypsilanti, MI, USA
  • Hammond, Scott
    • Affiliation: School of Medicine, Department of Cell Biology and Physiology
  • McLean, Samuel A
    • Affiliation: School of Medicine, Department of Anesthesiology
    • Other Affiliation: TRYUMPH Research Program, Chapel Hill, NC, USA
Abstract
  • Abstract Background Molecular mediators influencing the transition from acute to persistent musculoskeletal pain following common stress exposures such as motor vehicle collision (MVC) remain poorly understood. In this exploratory, proof of concept study, we compared circulating microRNA (miRNA) expression profiles in the early aftermath of MVC among individuals who did and did not subsequently develop persistent pain. Blood RNA samples were obtained from African American individuals (n = 53) who presented to the emergency department after MVC and were discharged to home after evaluation. The presence or absence of severe pain in the axial region, the most common and morbid region in which post-MVC pain occurs, was assessed 6 weeks following MVC via standardized questionnaire. miRNA expression was determined using miRNA-sequencing; nonparametric analyses were used to compare miRNA expression levels among individuals with and without persistent pain. Results Thirty-two mature miRNA were differentially expressed (p < 0.05) in those with and without severe axial pain at 6 weeks. miR-135a-5p, a regulator of the serotonin receptor that is known to be stress-responsive, differed most significantly between groups (p = 3 × 10−4). This miRNA, and miR-3613-3p (p = 0.001) survived correction for multiple testing (FDR = 0.15) in this small sample. Interestingly, differentially expressed miRNA were enriched for X chromosome location. In secondary analyses, the eight X chromosome miRNA were (a) more significantly associated with axial pain in women than men, (b) expressed more highly in the peripheral blood of women than men, and (c) predicted in pathway analyses (DIANA miRPath v 2.0) to regulate neuronal and neuroendocrine pathways previously implicated in various pain pathologies. Conclusions These results show that circulating miRNA predict persistent severe axial pain after MVC and suggest that they may be involved in the pathogenesis of post-traumatic musculoskeletal pain. However, further studies are needed to determine if these miRNA play a direct causal role.
Date of publication
DOI
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Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Linnstaedt et al.
Journal title
  • Molecular Pain
Journal volume
  • 11
Journal issue
  • 1
Page start
  • 66
Language
  • English
Bibliographic citation
  • Molecular Pain. 2015 Oct 24;11(1):66
Publisher
  • BioMed Central

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file details MicroRNA circulating in the early aftermath of motor vehicle collision predict persistent pain development and suggest a role for microRNA in sex-specific pain differences 2019-05-07 Public Download
file details Additional file 4: Table S4 2019-05-07 Public Download
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file details Additional file 1: Table S1 2019-05-07 Public Download
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