Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine
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Li, D, et al. Breadth of Sars-cov-2 Neutralization and Protection Induced by a Nanoparticle Vaccine. 2022. https://doi.org/10.17615/csn8-gx69APA
Li, D., Martinez, D., Schäfer, A., Chen, H., Barr, M., Sutherland, L., Lee, E., Parks, R., Mielke, D., Edwards, W., Newman, A., Bock, K., Minai, M., Nagata, B., Gagne, M., Douek, D., De Marco, C., Denny, T., Oguin, 3., Brown, A., Rountree, W., Wang, Y., Mansouri, K., Edwards, R., Ferrari, G., Sempowski, G., Eaton, A., Tang, J., Cain, D., Santra, S., Pardi, N., Weissman, D., Tomai, M., Fox, C., Moore, I., Andersen, H., Lewis, M., Golding, H., Seder, R., Khurana, S., Baric, R., Montefiori, D., Saunders, K., & Haynes, B. (2022). Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine. https://doi.org/10.17615/csn8-gx69Chicago
Li, D., D.R Martinez, A Schäfer, H Chen, M Barr, L.L Sutherland, E Lee et al. 2022. Breadth of Sars-Cov-2 Neutralization and Protection Induced by a Nanoparticle Vaccine. https://doi.org/10.17615/csn8-gx69- Creator
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Li, D.
- Other Affiliation: Duke University School of Medicine
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Martinez, D.R.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Schäfer, A.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Chen, H.
- Other Affiliation: Duke University School of Medicine
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Barr, M.
- Other Affiliation: Duke University School of Medicine
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Sutherland, L.L.
- Other Affiliation: Duke University School of Medicine
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Lee, E.
- Other Affiliation: Duke University School of Medicine
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Parks, R.
- Other Affiliation: Duke University School of Medicine
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Mielke, D.
- Other Affiliation: Duke University School of Medicine
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Edwards, W.
- Other Affiliation: Duke University School of Medicine
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Newman, A.
- Other Affiliation: Duke University School of Medicine
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Bock, K.W.
- Other Affiliation: National Institutes of Health
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Minai, M.
- Other Affiliation: National Institutes of Health
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Nagata, B.M.
- Other Affiliation: National Institutes of Health
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Gagne, M.
- Other Affiliation: National Institutes of Health
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Douek, D.C.
- Other Affiliation: National Institutes of Health
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DeMarco, C.T.
- Other Affiliation: Duke University School of Medicine
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Denny, T.N.
- Other Affiliation: Duke University School of Medicine
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Oguin, T.H., 3rd
- Other Affiliation: Duke University School of Medicine
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Brown, A.
- Other Affiliation: Duke University School of Medicine
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Rountree, W.
- Other Affiliation: Duke University School of Medicine
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Wang, Y.
- Other Affiliation: Duke University School of Medicine
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Mansouri, K.
- Other Affiliation: Duke University School of Medicine
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Edwards, R.J.
- Other Affiliation: Duke University School of Medicine
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Ferrari, G.
- Other Affiliation: Duke University School of Medicine
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Sempowski, G.D.
- Other Affiliation: Duke University School of Medicine
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Eaton, A.
- Other Affiliation: Duke University School of Medicine
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Tang, J.
- Other Affiliation: Food and Drug Administration
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Cain, D.W.
- Other Affiliation: Duke University School of Medicine
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Santra, S.
- Other Affiliation: Beth Israel Deaconess Medical Center
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Pardi, N.
- Other Affiliation: University of Pennsylvania
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Weissman, D.
- Other Affiliation: University of Pennsylvania
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Tomai, M.A.
- Other Affiliation: 3M Company
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Fox, C.B.
- Other Affiliation: Infectious Disease Research Institute
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Moore, I.N.
- Other Affiliation: National Institutes of Health
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Andersen, H.
- Other Affiliation: BIOQUAL
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Lewis, M.G.
- Other Affiliation: BIOQUAL
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Golding, H.
- Other Affiliation: Food and Drug Administration
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Seder, R.
- Other Affiliation: National Institutes of Health
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Khurana, S.
- Other Affiliation: Food and Drug Administration
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Baric, R.S.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Montefiori, D.C.
- Other Affiliation: Duke University School of Medicine
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Saunders, K.O.
- Other Affiliation: Duke University School of Medicine
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Haynes, B.F.
- Other Affiliation: Duke University School of Medicine
- Abstract
- Coronavirus vaccines that are highly effective against current and anticipated SARS-CoV-2 variants are needed to control COVID-19. We previously reported a receptor-binding domain (RBD)-sortase A-conjugated ferritin nanoparticle (scNP) vaccine that induced neutralizing antibodies against SARS-CoV-2 and pre-emergent sarbecoviruses and protected non-human primates (NHPs) from SARS-CoV-2 WA-1 infection. Here, we find the RBD-scNP induced neutralizing antibodies in NHPs against pseudoviruses of SARS-CoV and SARS-CoV-2 variants including 614G, Beta, Delta, Omicron BA.1, BA.2, BA.2.12.1, and BA.4/BA.5, and a designed variant with escape mutations, PMS20. Adjuvant studies demonstrate variant neutralization titers are highest with 3M-052-aqueous formulation (AF). Immunization twice with RBD-scNPs protect NHPs from SARS-CoV-2 WA-1, Beta, and Delta variant challenge, and protect mice from challenges of SARS-CoV-2 Beta variant and two other heterologous sarbecoviruses. These results demonstrate the ability of RBD-scNPs to induce broad neutralization of SARS-CoV-2 variants and to protect animals from multiple different SARS-related viruses. Such a vaccine could provide broad immunity to SARS-CoV-2 variants.
- Date of publication
- 2022
- DOI
- Identifier
- Resource type
- Article
- License
- Attribution 4.0 International
- Journal title
- Nature communications
- Journal volume
- 13
- Journal issue
- 1
- Page start
- 6309
- Language
- English
- Version
- Publisher
- ISSN
- 20411723
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