Nucleosome Turnover Regulates Histone Methylation Patterns over the Genome

Public Deposited
Analytics

Downloadable Content

Download PDF
Request Version for Screen Reader
Creator
  • Chory, E.J.
    • Other Affiliation: Stanford University School of Medicine
  • Calarco, J.P.
    • Other Affiliation: Stanford University School of Medicine
  • Hathaway, N.A.
    • Eshelman School of Pharmacy, Division of Chemical Biology and Medicinal Chemistry
  • Bell, O.
    • Other Affiliation: Vienna Biocenter
  • Neel, D.S.
    • Other Affiliation: Stanford University School of Medicine
  • Crabtree, G.R.
    • Other Affiliation: Stanford University School of Medicine
Abstract
  • Recent studies have indicated that nucleosome turnover is rapid, occurring several times per cell cycle. To access the effect of nucleosome turnover on the epigenetic landscape, we investigated H3K79 methylation, which is produced by a single methyltransferase (Dot1l) with no known demethylase. Using chemical-induced proximity (CIP), we find that the valency of H3K79 methylation (mono-, di-, and tri-) is determined by nucleosome turnover rates. Furthermore, propagation of this mark is predicted by nucleosome turnover simulations over the genome and accounts for the asymmetric distribution of H3K79me toward the transcriptional unit. More broadly, a meta-analysis of other conserved histone modifications demonstrates that nucleosome turnover models predict both valency and chromosomal propagation of methylation marks. Based on data from worms, flies, and mice, we propose that the turnover of modified nucleosomes is a general means of propagation of epigenetic marks and a determinant of methylation valence. © 2018Previous work has revealed that “writers” and “erasers” of histone modifications play a critical role in regulating gene expression. Now, studies by Chory et al. reveal that, in addition, the patterns, kinetics, and topology of histone modifications over the genome can be shaped by the rate of nucleosome turnover.
Date of publication
Keyword
DOI
Identifier
Resource type
  • Article
Rights statement
  • In Copyright
Journal title
  • Molecular Cell
Journal volume
  • 73
Journal issue
  • 1
Page start
  • 61
Page end
  • 72.e3
Language
  • English
ISSN
  • 1097-2765
Publisher
  • Cell Press

Relations

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
file details Nucleosome_turnover_regulates_histone_methylation.pdf 2022-04-13 Public Download