Chronic Methamphetamine Effects on Brain Structure and Function in Rats
Public DepositedAdd to collection
You do not have access to any existing collections. You may create a new collection.
Downloadable Content
Download PDFCitation
MLA
Thanos, Panayotis K, et al. Chronic Methamphetamine Effects On Brain Structure and Function In Rats. 2016. https://doi.org/10.17615/6dmq-7d35APA
Thanos, P., Kim, R., Delis, F., Ananth, M., Chachati, G., Rocco, M., Masad, I., Muniz, J., Grant, S., Gold, M., Cadet, J., & Volkow, N. (2016). Chronic Methamphetamine Effects on Brain Structure and Function in Rats. https://doi.org/10.17615/6dmq-7d35Chicago
Thanos, Panayotis K., Ronald Kim, Foteini Delis, Mala Ananth, George Chachati, Mark J Rocco, Ihssan Masad et al. 2016. Chronic Methamphetamine Effects On Brain Structure and Function In Rats. https://doi.org/10.17615/6dmq-7d35- Creator
-
Thanos, Panayotis K.
- Other Affiliation: Behavioral Neuropharmacology and Neuroimaging Laboratory on Addictions; Research Institute on Addictions; University at Buffalo
-
Kim, Ronald
- Affiliation: College of Arts and Sciences, Department of Psychology and Neuroscience
-
Delis, Foteini
- Other Affiliation: Department of Pharmacology; School of Medicine; University of Ioannina
-
Ananth, Mala
- Other Affiliation: Department of Neuroscience; Stony Brook University
-
Chachati, George
- Other Affiliation: Behavioral Neuropharmacology and Neuroimaging Laboratory on Addictions; Research Institute on Addictions; University at Buffalo
-
Rocco, Mark J.
- Other Affiliation: Behavioral Neuropharmacology and Neuroimaging Laboratory on Addictions; Research Institute on Addictions; University at Buffalo
-
Masad, Ihssan
- Other Affiliation: National High Magnetic Field Laboratory
-
Muniz, Jose A.
- Other Affiliation: National High Magnetic Field Laboratory
-
Grant, Samuel C.
- Other Affiliation: National High Magnetic Field Laboratory
-
Gold, Mark S.
- Other Affiliation: Washington University School of Medicine; Department of Psychiatry
-
Cadet, Jean Lud
- Other Affiliation: Molecular Neuropsychiatry Research Branch; NIDA; NIH; Department of Health and Human Services
-
Volkow, Nora D.
- Other Affiliation: Laboratory of Neuroimaging; NIAAA; NIH; Department of Health and Human Services
- Abstract
- Methamphetamine (MA) addiction is a growing epidemic worldwide. Chronic MA use has been shown to lead to neurotoxicity in rodents and humans. Magnetic resonance imaging (MRI) studies in MA users have shown enlarged striatal volumes and positron emission tomography (PET) studies have shown decreased brain glucose metabolism (BGluM) in the striatum of detoxified MA users. The present study examines structural changes of the brain, observes microglial activation, and assesses changes in brain function, in response to chronic MA treatment. Rats were randomly split into three distinct treatment groups and treated daily for four months, via i.p. injection, with saline (controls), or low dose (LD) MA (4 mg/kg), or high dose (HD) MA (8 mg/kg). Sixteen weeks into the treatment period, rats were injected with a glucose analog, [18F] fluorodeoxyglucose (FDG), and their brains were scanned with micro-PET to assess regional BGluM. At the end of MA treatment, magnetic resonance imaging at 21T was performed on perfused rats to determine regional brain volume and in vitro [3H]PK 11195 autoradiography was performed on fresh-frozen brain tissue to measure microglia activation. When compared with controls, chronic HD MA-treated rats had enlarged striatal volumes and increases in [3H]PK 11195 binding in striatum, the nucleus accumbens, frontal cortical areas, the rhinal cortices, and the cerebellar nuclei. FDG microPET imaging showed that LD MA-treated rats had higher BGluM in insular and somatosensory cortices, face sensory nucleus of the thalamus, and brainstem reticular formation, while HD MA-treated rats had higher BGluM in primary and higher order somatosensory and the retrosplenial cortices, compared with controls. HD and LD MA-treated rats had lower BGluM in the tail of the striatum, rhinal cortex, and subiculum and HD MA also had lower BGluM in hippocampus than controls. These results corroborate clinical findings and help further examine the mechanisms behind MA-induced neurotoxicity.
- Date of publication
- 2016
- Keyword
- DOI
- Identifier
- Publisher DOI: https://doi.org/10.1371/journal.pone.0155457
- PMCID: PMC4898739
- PMID: 27275601
- Onescience id: 9eb3440d22c39965d739480e46a6531794642fc3
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- PloS One
- Journal volume
- 11
- Journal issue
- 6
- Page start
- e0155457
- Language
- English
- ISSN
- 1932-6203
Relations
- Parents:
- In Collection:
This work has no parents.
Items
Thumbnail | Title | Date Uploaded | Visibility | Actions |
---|---|---|---|---|
PubMedCentral-PMC4898739.pdf | 2016 | Public | Download |