Bioluminescence imaging to track bacterial dissemination of Yersinia pestis using different routes of infection in mice Public Deposited

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Creator
  • Frothingham, Richard
    • Other Affiliation: Department of Medicine and Duke Human Vaccine Institute, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA
  • Weening, Eric H.
    • Affiliation: School of Medicine, Department of Genetics
  • Gonzalez, Rodrigo J.
    • Affiliation: School of Medicine, Department of Microbiology and Immunology
  • Miller, Virginia
    • Affiliation: School of Medicine, Department of Genetics, Department of Microbiology and Immunology
  • Sempowski, Gregory D.
    • Other Affiliation: Department of Medicine and Duke Human Vaccine Institute, Department of Pathology, Duke University Medical Center, Durham, NC, USA
Abstract
  • Abstract: Background: Plague is caused by Yersinia pestis, a bacterium that disseminates inside of the host at remarkably high rates. Plague bacilli disrupt normal immune responses in the host allowing for systematic spread that is fatal if left untreated. How Y. pestis disseminates from the site of infection to deeper tissues is unknown. Dissemination studies for plague are typically performed in mice by determining the bacterial burden in specific organs at various time points. To follow bacterial dissemination during plague infections in mice we tested the possibility of using bioluminescence imaging (BLI), an alternative non-invasive approach. Fully virulent Y. pestis was transformed with a plasmid containing the luxCDABE genes, making it able to produce light; this lux-expressing strain was used to infect mice by subcutaneous, intradermal or intranasal inoculation. Results: We successfully obtained images from infected animals and were able to follow bacterial dissemination over time for each of the three different routes of inoculation. We also compared the radiance signal from animals infected with a wild type strain and a Δcaf1ΔpsaA mutant that we previously showed to be attenuated in colonization of the lymph node and systemic dissemination. Radiance signals from mice infected with the wild type strain were larger than values obtained from mice infected with the mutant strain (linear regression of normalized values, P<0.05). Conclusions: We demonstrate that BLI is useful for monitoring dissemination from multiple inoculation sites, and for characterization of mutants with defects in colonization or dissemination.
Date of publication
Identifier
  • 22827851
  • doi:10.1186/1471-2180-12-147
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Rodrigo J Gonzalez et al.; licensee BioMed Central Ltd.
License
Journal title
  • BMC Microbiology
Journal volume
  • 12
Journal issue
  • 1
Page start
  • 147
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1471-2180
Bibliographic citation
  • BMC Microbiology. 2012 Jul 24;12(1):147
Access
  • Open Access
Publisher
  • BioMed Central Ltd
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