Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes

Public Deposited
Analytics

Downloadable Content

Download PDF
Request Version for Screen Reader
Creator
  • Voss, William N.
  • Hou, Yixuan J.
    • Affiliation: Gillings School of Global Public Health, Department of Epidemiology
  • Johnson, Nicole V.
  • Delidakis, George
  • Kim, Jin Eyun
  • Javanmardi, Kamyab
  • Horton, Andrew P.
  • Bartzoka, Foteini
  • Paresi, Chelsea J.
  • Tanno, Yuri
  • Chou, Chia-Wei
  • Abbasi, Shawn A.
  • Pickens, Whitney
  • George, Katia
  • Boutz, Daniel R.
  • Towers, Dalton M.
  • McDaniel, Jonathan R.
  • Billick, Daniel
  • Goike, Jule
  • Rowe, Lori
  • Batra, Dhwani
  • Pohl, Jan
  • Lee, Justin
  • Gangappa, Shivaprakash
  • Sambhara, Suryaprakash
  • Gadush, Michelle
  • Wang, Nianshuang
  • Person, Maria D.
  • Iverson, Brent L.
  • Gollihar, Jimmy D.
  • Dye, John
  • Herbert, Andrew
  • Finkelstein, Ilya J.
  • Baric, Ralph S.
    • Affiliation: Gillings School of Global Public Health, Department of Epidemiology
  • McLellan, Jason S.
  • Georgiou, George
  • Lavinder, Jason J.
  • Ippolito, Gregory C.
Abstract
  • The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies that circulate in blood plasma following SARS-CoV-2 infection are unknown. Proteomic deconvolution of the IgG repertoire to the spike glycoprotein in convalescent subjects revealed that the response is directed predominantly (>80%) against epitopes residing outside the receptor-binding domain (RBD). In one subject, just four IgG lineages accounted for 93.5% of the response, including an N-terminal domain (NTD)-directed antibody that was protective against lethal viral challenge. Genetic, structural, and functional characterization of a multi-donor class of “public” antibodies revealed an NTD epitope that is recurrently mutated among emerging SARS-CoV-2 variants of concern. These data show that “public” NTD-directed and other non-RBD plasma antibodies are prevalent and have implications for SARS-CoV-2 protection and antibody escape.
Date of publication
DOI
Identifier
Resource type
  • Article
Rights statement
  • In Copyright
Journal title
  • Science
ISSN
  • 0036-8075, 1095-9203

Relations

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details science.abg5268.full.pdf 2021-06-07 Public Download