Reverse Genetic Analysis of the Transcription Regulatory Sequence of the Coronavirus Transmissible Gastroenteritis Virus

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Creator
  • Curtis, Kristopher M.
    • Affiliation: School of Medicine, Department of Microbiology and Immunology
  • Yount, Boyd
    • Affiliation: Gillings School of Global Public Health, Department of Epidemiology
  • Sims, Amy C.
    • Affiliation: Gillings School of Global Public Health, Department of Epidemiology
  • Baric, Ralph S.
    • Affiliation: School of Medicine, Department of Microbiology and Immunology
Abstract
  • Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned CS elements for synthesis of subgenomic RNAs.
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  • Article
Rights statement
  • In Copyright
Journal title
  • Journal of Virology
Journal volume
  • 78
Journal issue
  • 11
Page start
  • 6061
Page end
  • 6066
Language
  • English
ISSN
  • 0022-538X

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