microRNA expression in the prefrontal cortex of individuals with schizophrenia and schizoaffective disorder Public Deposited

Downloadable Content

Download PDF
Creator
  • Jin, Jianping
    • Affiliation: School of Medicine, Curriculum in Genetics and Molecular Biology
  • Perkins, Diana
    • Affiliation: School of Medicine, Department of Psychiatry
  • Newman, Martin A
    • Affiliation: School of Medicine, Department of Cell Biology and Physiology
  • Jarskog, L Fredrik
    • Affiliation: School of Medicine, Department of Psychiatry
  • Thomson, J Michael
    • Affiliation: School of Medicine, Department of Cell Biology and Physiology
  • Woods, Keith
    • Affiliation: School of Medicine, Department of Cell Biology and Physiology
  • Parker, Joel S
    • Other Affiliation: Constella Group, LLC, Meridian Parkway, Durham, NC 27713, USA
  • Hammond, Scott
    • Affiliation: School of Medicine, Department of Cell Biology and Physiology
  • Jeffries, Clark D
    • Affiliation: Renaissance Computing Institute, Eshelman School of Pharmacy, School of Medicine
Abstract
  • Abstract Background microRNAs (miRNAs) are small, noncoding RNA molecules that are now thought to regulate the expression of many mRNAs. They have been implicated in the etiology of a variety of complex diseases, including Tourette's syndrome, Fragile × syndrome, and several types of cancer. Results We hypothesized that schizophrenia might be associated with altered miRNA profiles. To investigate this possibility we compared the expression of 264 human miRNAs from postmortem prefrontal cortex tissue of individuals with schizophrenia (n = 13) or schizoaffective disorder (n = 2) to tissue of 21 psychiatrically unaffected individuals using a custom miRNA microarray. Allowing a 5% false discovery rate, we found that 16 miRNAs were differentially expressed in prefrontal cortex of patient subjects, with 15 expressed at lower levels (fold change 0.63 to 0.89) and 1 at a higher level (fold change 1.77) than in the psychiatrically unaffected comparison subjects. The expression levels of 12 selected miRNAs were also determined by quantitative RT-PCR in our lab. For the eight miRNAs distinguished by being expressed at lower microarray levels in schizophrenia samples versus comparison samples, seven were also expressed at lower levels with quantitative RT-PCR. Conclusion This study is the first to find altered miRNA profiles in postmortem prefrontal cortex from schizophrenia patients.
Date of publication
Identifier
  • doi:10.1186/gb-2007-8-2-r27
  • 17326821
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Diana O Perkins et al.; licensee BioMed Central Ltd.
License
Journal title
  • Genome Biology
Journal volume
  • 8
Journal issue
  • 2
Page start
  • R27
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1465-6906
Bibliographic citation
  • Genome Biology. 2007 Feb 27;8(2):R27
Access
  • Open Access
Publisher
  • BioMed Central Ltd
Parents:

This work has no parents.

Items