Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein

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  • Zost, S.J.
    • Other Affiliation: Vanderbilt University Medical Center
  • Gilchuk, P.
    • Other Affiliation: Vanderbilt University Medical Center
  • Chen, R.E.
    • Other Affiliation: Washington University School of Medicine
  • Case, J.B.
    • Other Affiliation: Washington University School of Medicine
  • Reidy, J.X.
    • Other Affiliation: Vanderbilt University Medical Center
  • Trivette, A.
    • Other Affiliation: Vanderbilt University Medical Center
  • Nargi, R.S.
    • Other Affiliation: Vanderbilt University Medical Center
  • Sutton, R.E.
    • Other Affiliation: Vanderbilt University Medical Center
  • Suryadevara, N.
    • Other Affiliation: Vanderbilt University Medical Center
  • Chen, E.C.
    • Other Affiliation: Vanderbilt University Medical Center
  • Binshtein, E.
    • Other Affiliation: Vanderbilt University Medical Center
  • Shrihari, S.
    • Other Affiliation: Washington University School of Medicine
  • Ostrowski, M.
    • Other Affiliation: University of Toronto
  • Chu, H.Y.
    • Other Affiliation: University of Washington
  • Didier, J.E.
    • Other Affiliation: Berkeley Lights, Inc.
  • MacRenaris, K.W.
    • Other Affiliation: Berkeley Lights, Inc.
  • Jones, T.
    • Other Affiliation: Vanderbilt University Medical Center
  • Day, S.
    • Other Affiliation: Vanderbilt University Medical Center
  • Myers, L.
    • Other Affiliation: Vanderbilt University Medical Center
  • Eun-Hyung Lee, F.
    • Other Affiliation: Emory University
  • Nguyen, D.C.
    • Other Affiliation: Emory University
  • Sanz, I.
    • Other Affiliation: Emory University
  • Martinez, D.R.
    • Affiliation: Gillings School of Global Public Health, Department of Epidemiology
  • Rothlauf, P.W.
    • Other Affiliation: Washington University School of Medicine
  • Bloyet, L.-M.
    • Other Affiliation: Washington University School of Medicine
  • Whelan, S.P.J.
    • Other Affiliation: Washington University School of Medicine
  • Baric, R.S.
    • Affiliation: Gillings School of Global Public Health, Department of Epidemiology
  • Thackray, L.B.
    • Other Affiliation: Washington University School of Medicine
  • Diamond, M.S.
    • Other Affiliation: Washington University School of Medicine
  • Carnahan, R.H.
    • Other Affiliation: Vanderbilt University Medical Center
  • Crowe, J.E., Jr.
    • Other Affiliation: Vanderbilt University Medical Center
Abstract
  • Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused a global pandemic with millions of infections and hundreds of thousands of deaths to date1,2. In response, we used a rapid antibody discovery platform to isolate hundreds of human monoclonal antibodies (mAbs) against the SARS-CoV-2 spike (S) protein. We stratify these mAbs into five major classes on the basis of their reactivity to subdomains of S protein as well as their cross-reactivity to SARS-CoV. Many of these mAbs inhibit infection of authentic SARS-CoV-2 virus, with most neutralizing mAbs recognizing the receptor-binding domain (RBD) of S. This work defines sites of vulnerability on SARS-CoV-2 S and demonstrates the speed and robustness of advanced antibody discovery platforms.
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  • Article
Rights statement
  • In Copyright
Journal title
  • Nature Medicine
Journal volume
  • 26
Journal issue
  • 9
Page start
  • 1422
Page end
  • 1427
Language
  • English
ISSN
  • 1078-8956
Publisher
  • Nature Research

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