Molecular cloning of an enhancer binding protein:Isolation by screening of an expression library with a recognition site DNA Public Deposited

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Creator
  • Baldwin, Albert S., Jr.
    • ORCID: https://orcid.org/0000-0003-1246-1353
    • Other Affiliation: Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
  • LeBowitz, Jonathan H.
    • Other Affiliation: Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
  • Sharp, Phillip A.
    • Other Affiliation: Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
  • Singh, Harinder
    • Other Affiliation: Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Abstract
  • A novel strategy has been used to isolate a cDNA clone that encodes a DNA binding domain whose recognition properties overlap those of the mammalian transcription factors H2TF1 and NF-KB. These two factors are distinguished by their cell type distributions and their relative affinities for related sequence elements in the enhancers of the major histocompatibility complex (MHC) class I and immunoglobulin K chain genes. The human cDNA clone was detected by screening a ~ phage expression library with a binding site probe derived from the MHC enhancer. The phage encoded fusion protein binds specifically to both the MHC and K gene enhancers. The cDNA hybridizes to a single copy gene that is expressed as a 10 kb mRNA in both B and non-B cells. The strategy used in this study may prove generally useful in the cloning and analysis of sequence-specific DNA binding proteins.
Date of publication
Identifier
  • 2-s2.0-0024284826
  • doi:10.1016/S0092-8674(88)80034-5
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Resource type
  • Article
Rights statement
  • In Copyright
Journal title
  • Cell
Journal volume
  • 52
Journal issue
  • 3
Page start
  • 415
Page end
  • 423
Language
  • English
Version
  • Postprint
ISSN
  • 0092-8674
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