Racial Differences in Vaginal Fluid Metabolites and Association with Systemic Inflammation Markers among Ovarian Cancer Patients: A Pilot Study

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  • Osazuwa-Peters, Oyomoare L.
    • Other Affiliation: Duke University School of Medicine
  • Deveaux, April
    • Other Affiliation: Duke University School of Medicine
  • Muehlbauer, Michael J.
    • Other Affiliation: Duke University School of Medicine
  • Ilkayeva, Olga
    • Other Affiliation: Duke University School of Medicine
  • Bain, James R.
    • Other Affiliation: Duke University School of Medicine
  • Keku, Temitope
    • Affiliation: School of Medicine, Department of Medicine, Division of Gastroenterology and Hepatology
  • Berchuck, Andrew
    • Other Affiliation: Duke University School of Medicine
  • Huang, Bin
    • Other Affiliation: University of Kentucky
  • Ward, Kevin
    • Other Affiliation: Emory University
  • Gates Kuliszewski, Margaret
    • Other Affiliation: New York State Department of Health
  • Akinyemiju, Tomi
    • Other Affiliation: Duke University School of Medicine
Abstract
  • The vaginal microbiome differs by race and contributes to inflammation by directly producing or consuming metabolites or by indirectly inducing host immune response, but its potential contributions to ovarian cancer (OC) disparities remain unclear. In this exploratory cross-sectional study, we examine whether vaginal fluid metabolites differ by race among patients with OC, if they are associated with systemic inflammation, and if such associations differ by race. Study participants were recruited from the Ovarian Cancer Epidemiology, Healthcare Access, and Disparities Study between March 2021 and September 2022. Our study included 36 study participants with ovarian cancer who provided biospecimens; 20 randomly selected White patients and all 16 eligible Black patients, aged 50–70 years. Acylcarnitines (n = 45 species), sphingomyelins (n = 34), and ceramides (n = 21) were assayed on cervicovaginal fluid, while four cytokines (IL-1β, IL-10, TNF-α, and IL-6) were assayed on saliva. Seven metabolites showed >2-fold differences, two showed significant differences using the Wilcoxon rank-sum test (p < 0.05; False Discovery Rate > 0.05), and 30 metabolites had coefficients > ±0.1 in a Penalized Discriminant Analysis that achieved two distinct clusters by race. Arachidonoylcarnitine, the carnitine adduct of arachidonic acid, appeared to be consistently different by race. Thirty-eight vaginal fluid metabolites were significantly correlated with systemic inflammation biomarkers, irrespective of race. These findings suggest that vaginal fluid metabolites may differ by race, are linked with systemic inflammation, and hint at a potential role for mitochondrial dysfunction and sphingolipid metabolism in OC disparities. Larger studies are needed to verify these findings and further establish specific biological mechanisms that may link the vaginal microbiome with OC racial disparities.
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  • Article
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  • In Copyright
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  • Attribution 4.0 International
Journal title
  • Cancers
Journal volume
  • 16
Journal issue
  • 7
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  • 1259
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  • English
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  • 2072-6694
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  • MDPI

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