Affordable, Abbreviated Roche Monitor Assay for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma

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Creator
  • Fiscus, Susan A.
    • University of North Carolina at Chapel Hill
  • Chen, Shu
    • Other Affiliation: Johns Hopkins Bloomberg School of Public Health
  • Hoover, Donald
    • Other Affiliation: Rutgers University–New Brunswick
  • Kerkau, Melissa G.
    • University of North Carolina at Chapel Hill
  • Alabanza, Paul
    • University of North Carolina at Chapel Hill
  • Siharath, Som
    • University of North Carolina at Chapel Hill
  • Schmitz, John
    • University of North Carolina at Chapel Hill
  • Kumwenda, Newton
    • Other Affiliation: Johns Hopkins Bloomberg School of Public Health
  • Taha, Taha E.
    • Other Affiliation: Johns Hopkins Bloomberg School of Public Health
Abstract
  • The cost for the Roche Monitor assay kit can be reduced 50% by using only the 1:1, 1:25, and 1:625 human immunodeficiency virus (HIV) and the 1:1 quantitation standard dilutions. This abbreviated test applied to 1,774 mostly African samples had results nearly identical to those obtained following the package insert instructions. To make this approach feasible, Roche would have to provide additional lysis buffer and master mix.
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Resource type
  • Article
Rights statement
  • In Copyright
Journal title
  • Journal of Clinical Microbiology
Journal volume
  • 43
Journal issue
  • 8
Page start
  • 4200
Page end
  • 4202
Language
  • English
ISSN
  • 1098-660X
  • 1070-633X
  • 0095-1137

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