Influence of Different Levels of Lipoic Acid Synthase Gene Expression on Diabetic Nephropathy

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  • Xu, Longquan
    • Affiliation: School of Medicine, Department of Pathology and Laboratory Medicine
  • Hiller, Sylvia
    • Affiliation: School of Medicine, Department of Pathology and Laboratory Medicine
  • Simington, Stephen
    • Affiliation: School of Medicine, Department of Pathology and Laboratory Medicine
  • Nickeleit, Volker
    • Affiliation: School of Medicine, Department of Pathology and Laboratory Medicine
  • Maeda, Nobuyo
    • Affiliation: School of Medicine, Department of Pathology and Laboratory Medicine
  • James, Leighton R.
    • Other Affiliation: Division of Nephrology and Hypertension; Department of Medicine; University of Florida
  • Yi, Xianwen
    • Affiliation: School of Medicine, Department of Pathology and Laboratory Medicine
Abstract
  • Oxidative stress is implicated in the pathogenesis of diabetic nephropathy (DN) but outcomes of many clinical trials are controversial. To define the role of antioxidants in kidney protection during the development of diabetic nephropathy, we have generated a novel genetic antioxidant mouse model with over- or under-expression of lipoic acid synthase gene (Lias). These models have been mated with Ins2Akita/+ mice, a type I diabetic mouse model. We compare the major pathologic changes and oxidative stress status in two new strains of the mice with controls. Our results show that Ins2Akita/+ mice with under-expressed Lias gene, exhibit higher oxidative stress and more severe DN features (albuminuria, glomerular basement membrane thickening and mesangial matrix expansion). In contrast, Ins2Akita/+ mice with highly-expressed Lias gene display lower oxidative stress and less DN pathologic changes. Our study demonstrates that strengthening endogenous antioxidant capacity could be an effective strategy for prevention and treatment of DN.
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  • Article
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  • In Copyright
Journal title
  • PloS One
Journal volume
  • 11
Journal issue
  • 10
Page start
  • e0163208
Language
  • English
ISSN
  • 1932-6203

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