Creation of a cardiotropic adeno-associated virus: the story of viral directed evolution

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  • Yang, Lin
    • Other Affiliation: Wuhan Institute of Virology, Chinese Academy of Sciences, 44 Xiaohongshan, Wuhan, 430071, Hubei, China
  • Xiao, Xiao
    • Affiliation: Eshelman School of Pharmacy, Division of Pharmacoengineering and Molecular Pharmaceutics
Abstract
  • Abstract Adeno-associated virus (AAV) is an important vector system for human gene therapy. Although use of AAV serotypes can result in efficient myocardial gene transfer, improvements in the transduction efficiency and specificity are still required. As a method for artificial modification and selection of gene function, directed evolution has been used for diverse applications in genetic engineering of enzymes and proteins. Since 2000, pioneering work has been performed on directed evolution of viral vectors. We further attempted to evolve the AAV using DNA shuffling and in vivo biopanning in a mouse model. An AAVM41 mutant was characterized, which was found to have improved transduction efficiency and specificity in myocardium, an attribute unknown for any natural AAV serotypes. This review focuses on the development of AAV vector for cardiac gene transfer, the history of directed evolution of viral vectors, and our creation of a cardiotropic AAV, which might have implications for the future design and application of viral vectors.
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  • Article
Rights statement
  • In Copyright
Rights holder
  • Lin Yang et al.; licensee BioMed Central Ltd.
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Journal title
  • Virology Journal
Journal volume
  • 10
Journal issue
  • 1
Page start
  • 50
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1743-422X
Bibliographic citation
  • Virology Journal. 2013 Feb 11;10(1):50
Publisher
  • BioMed Central Ltd
Access right
  • Open Access
Date uploaded
  • February 15, 2013

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