Evidence for compensatory upregulation of expressed X-linked genes in mammals, Caenorhabditis elegans and Drosophila melanogaster Public Deposited
- Creator
- Deng, Xinxian
- Hiatt, Joseph B
- Nguyen, Di Kim
- Ercan, Sevinc
- Sturgill, David
- Hillier, LaDeana W
- Schlesinger, Felix
- Davis, Carrie A
- Reinke, Valerie J
- Gingeras, Thomas R
- Shendure, Jay
- Waterston, Robert H
- Oliver, Brian
- Lieb, Jason D
- Disteche, Christine M
- Abstract
- Many animal species use a chromosome-based mechanism of sex determination, which has led to the coordinate evolution of dosage-compensation systems. Dosage compensation not only corrects the imbalance in the number of X chromosomes between the sexes but also is hypothesized to correct dosage imbalance within cells that is due to monoallelic X-linked expression and biallelic autosomal expression, by upregulating X-linked genes twofold (termed ‘Ohno’s hypothesis’). Although this hypothesis is well supported by expression analyses of individual X-linked genes and by microarray-based transcriptome analyses, it was challenged by a recent study using RNA sequencing and proteomics. We obtained new, independent RNA-seq data, measured RNA polymerase distribution and reanalyzed published expression data in mammals, C. elegans and Drosophila. Our analyses, which take into account the skewed gene content of the X chromosome, support the hypothesis of upregulation of expressed X-linked genes to balance expression of the genome.
- Date of publication
- 2011
- Keyword
- Invertebrata
- Gene expression
- Nematoda
- Caenorhabditis elegans
- Gene
- Caractère lié au sexe
- Gen
- Sex linked character
- Nemathelminthia
- Cromosoma X
- Mammalia
- Insecta
- Diptera
- Chromosome X
- Arthropoda
- Drosophilidae
- Carácter ligado al sexo
- Vertebrata
- Expresión genética
- Gène
- X-Chromosome
- Drosophila melanogaster
- Expression génique
- Helmintha
- DOI
- Identifier
- Publisher DOI: https://doi.org/10.1038/ng.948
- PMCID: PMC3576853
- PMID: 22019781
- Onescience id: d7b26caf07a648819b0126827ea022057b85cc1a
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Nature Genetics
- Journal volume
- 43
- Journal issue
- 12
- Language
- English
- ISSN
- 1061-4036
- 1546-1718
- Parents:
This work has no parents.
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