The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients
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Premkumar, Lakshmanane, et al. The Receptor Binding Domain of the Viral Spike Protein Is an Immunodominant and Highly Specific Target of Antibodies In Sars-cov-2 Patients. 2020. https://doi.org/10.17615/1wsx-jv69APA
Premkumar, L., Segovia Chumbez, B., Jadi, R., Martinez, D., Raut, R., Markmann, A., Cornaby, C., Bartlet, L., Weiss, S., Park, Y., Edwards, C., Weimer, E., Scherer, E., Rouphael, N., Edupuganti, S., Weiskopf, D., Tse, L., Hou, Y., Margolis, D., Sette, A., Collins, M., Schmitz, J., Baric, R., & De Silva, A. (2020). The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients. https://doi.org/10.17615/1wsx-jv69Chicago
Premkumar, Lakshmanane, Bruno Segovia Chumbez, Ramesh Jadi, David R Martinez, Rajendra Raut, Alena Markmann, Caleb Cornaby et al. 2020. The Receptor Binding Domain of the Viral Spike Protein Is an Immunodominant and Highly Specific Target of Antibodies In Sars-Cov-2 Patients. https://doi.org/10.17615/1wsx-jv69- Creator
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Premkumar, Lakshmanane
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Segovia-Chumbez, Bruno
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Jadi, Ramesh
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Martinez, David R.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Raut, Rajendra
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Markmann, Alena
- Affiliation: School of Medicine, Department of Medicine
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Cornaby, Caleb
- Affiliation: School of Medicine
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Bartlet, Luther
- Affiliation: School of Medicine, Department of Medicine
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Weiss, Susan
- Affiliation: School of Medicine, Department of Medicine
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Park, Yara
- Affiliation: School of Medicine, Department of Medicine
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Edwards, Caitlin E.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Weimer, Eric
- Affiliation: School of Medicine, Department of Pathology and Laboratory Medicine
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Scherer, Erin M.
- Other Affiliation: Emory University
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Rouphael, Nadine
- Other Affiliation: Emory University
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Edupuganti, Srilatha
- Other Affiliation: Emory University
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Weiskopf, Daniela
- Other Affiliation: La Jolla Institute for Immunology
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Tse, Longping V.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Hou, Yixuan J.
- Affiliation: Gillings School of Global Public Health, Department of Epidemiology
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Margolis, David
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Sette, Alessandro
- Other Affiliation: La Jolla Institute for Immunology
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Collins, Matthew H.
- Other Affiliation: Emory University
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Schmitz, John
- Affiliation: School of Medicine, Department of Pathology and Laboratory Medicine
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Baric, Ralph S.
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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de Silva, Aravinda
- Affiliation: School of Medicine, Department of Microbiology and Immunology
- Abstract
- The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that first emerged in late 2019 is responsible for a pandemic of severe respiratory illness. People infected with this highly contagious virus can present with clinically inapparent, mild, or severe disease. Currently, the virus infection in individuals and at the population level is being monitored by PCR testing of symptomatic patients for the presence of viral RNA. There is an urgent need for SARS-CoV-2 serologic tests to identify all infected individuals, irrespective of clinical symptoms, to conduct surveillance and implement strategies to contain spread. As the receptor binding domain (RBD) of the spike protein is poorly conserved between SARS-CoVs and other pathogenic human coronaviruses, the RBD represents a promising antigen for detecting CoV-specific antibodies in people. Here we use a large panel of human sera (63 SARS-CoV-2 patients and 71 control subjects) and hyperimmune sera from animals exposed to zoonotic CoVs to evaluate RBD's performance as an antigen for reliable detection of SARS-CoV-2-specific antibodies. By day 9 after the onset of symptoms, the recombinant SARS-CoV-2 RBD antigen was highly sensitive (98%) and specific (100%) for antibodies induced by SARS-CoVs. We observed a strong correlation between levels of RBD binding antibodies and SARS-CoV-2 neutralizing antibodies in patients. Our results, which reveal the early kinetics of SARS-CoV-2 antibody responses, support using the RBD antigen in serological diagnostic assays and RBD-specific antibody levels as a correlate of SARS-CoV-2 neutralizing antibodies in people.
- Date of publication
- 2020
- DOI
- Identifier
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Science Immunology
- Journal volume
- 5
- Journal issue
- 48
- Language
- English
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