Estimation of 18S gene copy number in marine eukaryotic plankton using a next-generation sequencing approach Public Deposited

Downloadable Content

Download PDF
Creator
  • Gong, W.
    • Affiliation: University of North Carolina at Chapel Hill
  • Marchetti, A.
    • Affiliation: University of North Carolina at Chapel Hill
Abstract
  • The small subunit 18S rRNA (18S) gene is the most commonly used marker for taxonomic identification in eukaryotes. However, protists may harbor substantial variation in their 18S gene copy number, which can lead to a rapid decline in concordance between 18S gene sequences and actual organismal abundances. Here we used a computational method to estimate 18S gene copy number in seven species of marine eukaryotic phytoplankton and found strong interspecies and strain-level differences across and within the examined species. Our results emphasize that variations in 18S gene copy number need to be taken into consideration and that corrections can improve the accuracy of quantitative eukaryotic microbial community profiles.
Date of publication
Keyword
DOI
Identifier
Resource type
  • Article
Rights statement
  • In Copyright
License
  • Attributon 4.0 International
Journal title
  • Frontiers in Marine Science
Journal volume
  • 6
Journal issue
  • APR
Language
  • English
Version
  • Publisher
ISSN
  • 2296-7745
Publisher
  • Frontiers Media S.A.
Parents:

This work has no parents.

In Collection:

Items