Two unequally redundant "helper" immune receptor families mediate Arabidopsis thaliana intracellular "sensor" immune receptor functions
Public Deposited- Creator
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Saile, S.C.
- Other Affiliation: University of Tübingen
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Jacob, P.
- Affiliation: College of Arts and Sciences, Department of Biology
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Castel, B.
- Other Affiliation: Sainsbury Laboratory
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Jubic, L.M.
- Affiliation: College of Arts and Sciences, Department of Biology
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Salas-Gonzáles, I.
- Affiliation: College of Arts and Sciences, Department of Biology
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Bäcker, M.
- Other Affiliation: University of Tübingen
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Jones, J.D.G.
- Other Affiliation: Sainsbury Laboratory
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Dangl, J.L.
- Affiliation: College of Arts and Sciences, Department of Biology
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El Kasmi, F.
- Other Affiliation: University of Tübingen
- Abstract
- Plant nucleotide-binding (NB) leucine-rich repeat (LRR) receptor (NLR) proteins function as intracellular immune receptors that perceive the presence of pathogen-derived virulence proteins (effectors) to induce immune responses. The 2 major types of plant NLRs that "sense"pathogen effectors differ in their N-terminal domains: these are Toll/interleukin-1 receptor resistance (TIR) domain-containing NLRs (TNLs) and coiled-coil (CC) domain-containing NLRs (CNLs). In many angiosperms, the RESISTANCE TO POWDERY MILDEW 8 (RPW8)-CC domain containing NLR (RNL) subclass of CNLs is encoded by 2 gene families, ACTIVATED DISEASE RESISTANCE 1 (ADR1) and N REQUIREMENT GENE 1 (NRG1), that act as "helper"NLRs during multiple sensor NLR-mediated immune responses. Despite their important role in sensor NLR-mediated immunity, knowledge of the specific, redundant, and synergistic functions of helper RNLs is limited. We demonstrate that the ADR1 and NRG1 families act in an unequally redundant manner in basal resistance, effector-triggered immunity (ETI) and regulation of defense gene expression. We define RNL redundancy in ETI conferred by some TNLs and in basal resistance against virulent pathogens. We demonstrate that, in Arabidopsis thaliana, the 2 RNL families contribute specific functions in ETI initiated by specific CNLs and TNLs. Time-resolved whole genome expression profiling revealed that RNLs and "classical"CNLs trigger similar transcriptome changes, suggesting that RNLs act like other CNLs to mediate ETI downstream of sensor NLR activation. Together, our genetic data confirm that RNLs contribute to basal resistance, are fully required for TNL signaling, and can also support defense activation during CNL-mediated ETI.
- Date of publication
- 2020
- Keyword
- NLR Proteins
- immunoglobulin receptor
- nonhuman
- n requirement gene 1
- Gene Expression Regulation, Plant
- plant disease
- nucleotide binding oligomerization domain like receptor
- Plant Immunity
- genetics
- plant immunity
- Receptors, Immunologic
- Arabidopsis protein
- whole genome sequencing
- Arabidopsis
- receptor
- multigene family
- signal peptide
- Plants, Genetically Modified
- gene expression
- signal transduction
- disease resistance
- virulence
- ADR1 gene
- Transcriptome
- transcriptome
- unclassified drug
- Plant Diseases
- controlled study
- amino terminal sequence
- gene expression profiling
- AT5G66900 protein, Arabidopsis
- AT5G66910 protein, Arabidopsis
- Signal Transduction
- transgenic plant
- plant gene
- interleukin 1 receptor
- angiosperm
- Intracellular Signaling Peptides and Proteins
- physiology
- Arabidopsis Proteins
- protein function
- Article
- activated disease resistance 1
- ADR1 protein, Arabidopsis
- gene expression regulation
- immunology
- Arabidopsis thaliana
- immune receptor
- NRG1 gene
- Disease Resistance
- Multigene Family
- DOI
- Identifier
- https://dx.doi.org/10.1371/journal.pbio.3000783
- PMID 32925907
- Resource type
- Article
- Rights statement
- In Copyright
- License
- Attribution 4.0 International
- Journal title
- PLoS Biology
- Journal volume
- 18
- Journal issue
- 9
- Language
- English
- Version
- Publisher
- ISSN
- 1544-9173
- Publisher
- Public Library of Science
Relations
- Parents:
- In Collection:
This work has no parents.
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