Luminal and Mucosal-Associated Intestinal Microbiota in Patients with Diarrhea-Predominant Irritable Bowel Syndrome Public Deposited

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Creator
  • Carroll, Ian
    • Affiliation: Center for Gastrointestinal Biology and Disease, School of Medicine, Department of Medicine, Division of Gastroenterology and Hepatology
  • Sartor, Balfour
    • Affiliation: Center for Gastrointestinal Biology and Disease, School of Medicine, Department of Medicine, Division of Gastroenterology and Hepatology
  • Ringel, Yehuda
    • Affiliation: Center for Gastrointestinal Biology and Disease, School of Medicine, Department of Medicine, Division of Gastroenterology and Hepatology
  • Chang, Young-Hyo
    • Other Affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), Dagjon, Korea
  • Park, Jiwon
    • Affiliation: Center for Gastrointestinal Biology and Disease, School of Medicine, Department of Medicine, Division of Gastroenterology and Hepatology
Abstract
  • Abstract Background Recent studies have suggested a role for an altered intestinal microbiota in the pathophysiology of irritable bowel syndrome (IBS). However, no consensus has been reached regarding the association between specific enteric bacterial groups and IBS. The aim of this study was to investigate the fecal and mucosal-associated microbiota using two independent techniques in intestinal samples from diarrhea-predominant IBS (D-IBS) and healthy controls. Methods Fecal and colonic mucosal biopsy samples were obtained from 10 D-IBS patients and 10 healthy controls. Colonic tissue was collected during a un-sedated un-prepped flexible sigmoidoscopy. Fecal and tissue samples were processed immediately upon collection for culture under aerobic and anaerobic conditions or frozen for further molecular analysis. DNA was extracted from all frozen samples and used to enumerate specific bacterial groups using quantitative real-time PCR (qPCR). Results Culture analysis of intestinal samples demonstrated a significant reduction in the concentration of aerobic bacteria in fecal samples from D-IBS patients when compared to healthy controls (1.4 × 107 vs. 8.4 × 108 CFUs/g feces, P = 0.002). qPCR analysis demonstrated a significant 3.6 fold increase (P = 0.02) in concentrations of fecal Lactobacillus species between D-IBS patients and healthy controls. Conclusions Our culture and molecular data indicate that quantitative differences exist in specific bacterial groups in the microbiota between D-IBS and healthy subjects.
Date of publication
Identifier
  • doi:10.1186/1757-4749-2-19
  • 21143915
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Ian M Carroll et al.; licensee BioMed Central Ltd.
Journal title
  • Gut Pathogens
Journal volume
  • 2
Journal issue
  • 1
Page start
  • 19
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1757-4749
Bibliographic citation
  • Gut Pathogens. 2010 Dec 09;2(1):19
Access
  • Open Access
Publisher
  • BioMed Central Ltd
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