The F-box protein Cdc4/Fbxw7 is a novel regulator of neural crest development in Xenopus laevis Public Deposited

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Creator
  • Hartley, Rebecca S
    • Other Affiliation: Department of Cell Biology and Physiology, and Cancer Center, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA
  • Wise, Helen M
    • Other Affiliation: Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QP, UK
  • Almeida, Alexandra D
    • Other Affiliation: Department of Oncology, University of Cambridge, Hutchison-MRC Research Centre, Addenbrookes Hospital, Hills Road, Cambridge, CB2 0XZ, UK
  • Philpott, Anna
    • Other Affiliation: Department of Oncology, University of Cambridge, Hutchison-MRC Research Centre, Addenbrookes Hospital, Hills Road, Cambridge, CB2 0XZ, UK
  • Hindley, Christopher J
    • Other Affiliation: Department of Oncology, University of Cambridge, Hutchison-MRC Research Centre, Addenbrookes Hospital, Hills Road, Cambridge, CB2 0XZ, UK
  • Slevin, Michael K
    • Affiliation: School of Medicine, Integrative Program for Biological and Genome Sciences
Abstract
  • Abstract Background The neural crest is a unique population of cells that arise in the vertebrate ectoderm at the neural plate border after which they migrate extensively throughout the embryo, giving rise to a wide range of derivatives. A number of proteins involved in neural crest development have dynamic expression patterns, and it is becoming clear that ubiquitin-mediated protein degradation is partly responsible for this. Results Here we demonstrate a novel role for the F-box protein Cdc4/Fbxw7 in neural crest development. Two isoforms of Xenopus laevis Cdc4 were identified, and designated xCdc4α and xCdc4β. These are highly conserved with vertebrate Cdc4 orthologs, and the Xenopus proteins are functionally equivalent in terms of their ability to degrade Cyclin E, an established vertebrate Cdc4 target. Blocking xCdc4 function specifically inhibited neural crest development at an early stage, prior to expression of c-Myc, Snail2 and Snail. Conclusions We demonstrate that Cdc4, an ubiquitin E3 ligase subunit previously identified as targeting primarily cell cycle regulators for proteolysis, has additional roles in control of formation of the neural crest. Hence, we identify Cdc4 as a protein with separable but complementary functions in control of cell proliferation and differentiation.
Date of publication
Identifier
  • doi:10.1186/1749-8104-5-1
  • 20047651
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Alexandra D Almeida et al.; licensee BioMed Central Ltd.
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Journal title
  • Neural Development
Journal volume
  • 5
Journal issue
  • 1
Page start
  • 1
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1749-8104
Bibliographic citation
  • Neural Development. 2010 Jan 04;5(1):1
Access
  • Open Access
Publisher
  • BioMed Central Ltd
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