Broad neutralization of SARS-related viruses by human monoclonal antibodies

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  • Wec, Anna Z.
  • Wrapp, Daniel
  • Herbert, Andrew S.
  • Maurer, Daniel P.
  • Haslwanter, Denise
  • Sakharkar, Mrunal
  • Jangra, Rohit K.
  • Dieterle, M. Eugenia
  • Lilov, Asparouh
  • Huang, Deli
  • Tse, Longping V.
    • Affiliation: Gillings School of Global Public Health, Department of Epidemiology
  • Johnson, Nicole V.
  • Hsieh, Ching-Lin
  • Wang, Nianshuang
  • Nett, Juergen H.
  • Champney, Elizabeth
  • Burnina, Irina
  • Brown, Michael
  • Lin, Shu
  • Sinclair, Melanie
  • Johnson, Carl
  • Fels, J. Maximilian
  • O'Brien, Cecilia M.
  • Graham, Barney S.
  • Nemazee, David
  • Burton, Dennis
  • Baric, Ralph S.
    • Affiliation: School of Medicine, Department of Microbiology and Immunology
  • Voss, James E.
  • Chandran, Kartik
  • Dye, John M.
  • McLellan, Jason S.
  • Walker, Laura M.
Abstract
  • Broadly protective vaccines against known and preemergent human coronaviruses (HCoVs) are urgently needed. To gain a deeper understanding of cross-neutralizing antibody responses, we mined the memory B cell repertoire of a convalescent severe acute respiratory syndrome (SARS) donor and identified 200 SARS coronavirus 2 (SARS-CoV-2) binding antibodies that target multiple conserved sites on the spike (S) protein. A large proportion of the non-neutralizing antibodies display high levels of somatic hypermutation and cross-react with circulating HCoVs, suggesting recall of preexisting memory B cells elicited by prior HCoV infections. Several antibodies potently cross-neutralize SARS-CoV, SARS-CoV-2, and the bat SARS-like virus WIV1 by blocking receptor attachment and inducing S1 shedding. These antibodies represent promising candidates for therapeutic intervention and reveal a target for the rational design of pan-sarbecovirus vaccines.
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  • Article
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  • In Copyright
Journal title
  • Science
Journal volume
  • 369
Journal issue
  • 6504
Page start
  • 731
Page end
  • 736
Language
  • English

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