Drosophila, which lacks canonical transcription-coupled repair proteins, performs transcription-coupled repair
Public Deposited- Creator
-
Deger, Nazli
- College of Arts and Sciences, Department of Biology
-
Yang, Yanyan
- ORCID: https://orcid.org/0000-0001-9943-4279
- School of Medicine, Department of Biochemistry and Biophysics
-
Lindsey-Boltz, Laura A
- ORCID: https://orcid.org/0000-0001-6493-8194
- School of Medicine, Department of Biochemistry and Biophysics
-
Sancar, Aziz
- School of Medicine, Department of Biochemistry and Biophysics
-
Selby, Christopher P
- School of Medicine, Department of Biochemistry and Biophysics
-
Deger, Nazli
- Abstract
- Previous work with the classic T4 endonuclease V digestion of DNA from irradiated Drosophila cells followed by Southern hybridization led to the conclusion that Drosophila lacks transcription-coupled repair (TCR). This conclusion was reinforced by the Drosophila Genome Project, which revealed that Drosophila lacks Cockayne syndrome WD repeat protein (CSA), CSB, or UV-stimulated scaffold protein A (UVSSA) homologs, whose orthologs are present in eukaryotes ranging from Arabidopsis to humans that carry out TCR. A recently developed in vivo excision assay and the excision repair-sequencing (XR-Seq) method have enabled genome-wide analysis of nucleotide excision repair in various organisms at single-nucleotide resolution and in a strand-specific manner. Using these methods, we have discovered that Drosophila S2 cells carry out robust TCR comparable with that observed in mammalian cells. Our findings provide critical new insights into the mechanisms of TCR among various different species.
- Date of publication
- October 17, 2019
- Keyword
- previous work
- project
- Southern
- transcription-coupled repair
- repair sequences
- mammalian cells
- organization
- species
- Genome Project
- assay
- UV-stimulated scaffold protein A
- protein A
- repeat proteins
- findings
- CSB
- genome-wide analysis
- Southern hybridization
- DNA
- resolution
- excision repair
- cells
- homology
- excision repair sequencing
- Drosophila
- WD-repeat protein
- eukaryotes
- mechanisms of transcription-coupled repair
- single-nucleotide resolution
- excision
- orthologs
- hybrid
- protein
- manner
- repair
- nucleotide excision repair
- excision assay
- work
- genome
- mechanism
- humans
- strand-specific manner
- method
- analysis of nucleotide excision repair
- repair proteins
- DOI
- Identifier
- PMID: 31624146
- Dimensions ID: pub.1121888577
- DOI: https://dx.doi.org/10.1074/jbc.ac119.011448
- PMCID: PMC6885609
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Journal of Biological Chemistry
- Journal volume
- 294
- Journal issue
- 48
- Page start
- 18092
- Page end
- 18098
- Funder
- National Institute of General Medical Sciences
- Office of the Director
- National Institute of Environmental Health Sciences
- ISSN
- 0021-9258
- 1083-351X
- Publisher
- Elsevier
Relations
- Parents:
- In Collection:
This work has no parents.
Items
| Thumbnail | Title | Date Uploaded | Visibility | Actions |
|---|---|---|---|---|
|
|
zbc18092.pdf | 2024-08-26 | Public | Download |