Possible Mechanism for Cold Denaturation of Proteins at High Pressure
Public Deposited- Creator
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Marqués, Manuel I.
- Other Affiliation: Boston University
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Borreguero, Jose M.
- Other Affiliation: Boston University
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Stanley, H. Eugene
- Other Affiliation: Boston University
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Dokholyan, Nikolay V.
- School of Medicine
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Marqués, Manuel I.
- Abstract
- We study cold denaturation of proteins at high pressures. Using multicanonical Monte Carlo simulations of a model protein in a water bath, we investigate the effect of water density fluctuations on protein stability. We find that above the pressure where water freezes to the dense ice phase (approximately 2 kbars) the mechanism for cold denaturation with decreasing temperature is the loss of local low-density water structure. We find our results in agreement with data of bovine pancreatic ribonuclease A.
- Date of publication
- 2003
- Keyword
- DOI
- Identifier
- Publisher DOI: https://doi.org/10.1103/PhysRevLett.91.138103
- Onescience id: d8a448250ac3e3d57f9804e9b146494638a77735
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Physical Review Letters
- Journal volume
- 91
- Journal issue
- 13
- Language
- English
- ISSN
- 1079-7114
- 0031-9007
- 1092-0145
Relations
- Parents:
This work has no parents.
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