Distinct SOX9 levels differentially mark stem/progenitor populations and enteroendocrine cells of the small intestine epithelium Public Deposited
- Creator
-
Formeister, Eric J.
- Affiliation: School of Medicine, Department of Medicine
- Magness, Scott T.
- Abstract
- SOX transcription factors have the capacity to modulate stem/progenitor cell proliferation and differentiation in a dose-dependent manner. SOX9 is expressed in the small intestine epithelial stem cell zone. Therefore, we hypothesized that differential levels of SOX9 may exist, influencing proliferation and/or differentiation of the small intestine epithelium. Sox9 expression levels in the small intestine were investigated using a Sox9 enhanced green fluorescent protein (Sox9EGFP) transgenic mouse. Sox9EGFP levels correlate with endogenous SOX9 levels, which are expressed at two steady-state levels, termed Sox9EGFPLO and Sox9EGFPHI. Crypt-based columnar cells are Sox9EGFPLO and demonstrate enriched expression of the stem cell marker, Lgr5. Sox9EGFPHI cells express chromogranin A and substance P but do not express Ki67 and neurogenin3, indicating that Sox9EGFPHI cells are postmitotic enteroendocrine cells. Overexpression of SOX9 in a crypt cell line stopped proliferation and induced morphological changes. These data support a bimodal role for SOX9 in the intestinal epithelium, where low SOX9 expression supports proliferative capacity, and high SOX9 expression suppresses proliferation.
- Date of publication
- May 2009
- Keyword
- DOI
- Identifier
- Related resource URL
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Gastrointestinal and Liver Physiology
- Journal volume
- 296
- Journal issue
- 5
- Language
- English
- Version
- Postprint
- Parents:
This work has no parents.
Items
Thumbnail | Title | Date Uploaded | Visibility | Actions |
---|---|---|---|---|
|
Distinct SOX9 Levels.pdf | 2019-05-03 | Public |
|