EGFR and EGFRvIII undergo stress- and EGFR kinase inhibitor-induced mitochondrial translocalization: A potential mechanism of EGFR-driven antagonism of apoptosis Public Deposited

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Creator
  • Lo, Hui-Wen
    • Other Affiliation: Division of Surgical Sciences, Department of Surgery, Duke University School of Medicine, 433A MSRB I, 103 Research Drive, Durham, NC 27710, USA; Duke Comprehensive Cancer Center, Durham, NC 27710, USA; Preston Robert Tisch Brain Tumor Center at Duke University, Durham, NC 27710, USA
  • Zhu, Hu
    • Affiliation: School of Medicine, Department of Pharmacology
    • Other Affiliation: Division of Surgical Sciences, Department of Surgery, Duke University School of Medicine, 433A MSRB I, 103 Research Drive, Durham, NC 27710, USA
  • Ali-Osman, Francis
    • Other Affiliation: Division of Surgical Sciences, Department of Surgery, Duke University School of Medicine, 433A MSRB I, 103 Research Drive, Durham, NC 27710, USADuke Comprehensive Cancer Center, Durham, NC 27710, USA; Preston Robert Tisch Brain Tumor Center at Duke University, Durham, NC 27710, USA
  • Cao, Xinyu
    • Other Affiliation: Division of Surgical Sciences, Department of Surgery, Duke University School of Medicine, 433A MSRB I, 103 Research Drive, Durham, NC 27710, USA
Abstract
  • Abstract Background Epidermal growth factor receptor (EGFR) plays an essential role in normal development, tumorigenesis and malignant biology of human cancers, and is known to undergo intracellular trafficking to subcellular organelles. Although several studies have shown that EGFR translocates into the mitochondria in cancer cells, it remains unclear whether mitochondrially localized EGFR has an impact on the cells and whether EGFRvIII, a constitutively activated variant of EGFR, undergoes mitochondrial transport similar to EGFR. Results We report that both receptors translocate into the mitochondria of human glioblastoma and breast cancer cells, following treatments with the apoptosis inducers, staurosporine and anisomycin, and with an EGFR kinase inhibitor. Using mutant EGFR/EGFRvIII receptors engineered to undergo enriched intracellular trafficking into the mitochondria, we showed that glioblastoma cells expressing the mitochondrially enriched EGFRvIII were more resistant to staurosporine- and anisomycin-induced growth suppression and apoptosis and were highly resistant to EGFR kinase inhibitor-mediated growth inhibition. Conclusions These findings indicate that apoptosis inducers and EGFR-targeted inhibitors enhance mitochondrial translocalization of both EGFR and EGFRvIII and that mitochondrial accumulation of these receptors contributes to tumor drug resistance. The findings also provide evidence for a potential link between the mitochondrial EGFR pathway and apoptosis.
Date of publication
Identifier
  • 21388543
  • doi:10.1186/1476-4598-10-26
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Xinyu Cao et al.; licensee BioMed Central Ltd.
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Journal title
  • Molecular Cancer
Journal volume
  • 10
Journal issue
  • 1
Page start
  • 26
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1476-4598
Bibliographic citation
  • Molecular Cancer. 2011 Mar 09;10(1):26
Access
  • Open Access
Publisher
  • BioMed Central Ltd
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