Laser-scanning velocimetry: A confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae Public Deposited

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Creator
  • Sciaky, Noah
    • Affiliation: School of Medicine, Department of Pharmacology, N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
  • Hahn, Klaus
    • Affiliation: School of Medicine, Department of Pharmacology, N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
  • Linney, Elwood
    • Other Affiliation: Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27708, USA
  • Stalheim, Lisa
    • Affiliation: School of Medicine, Department of Pharmacology, N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
  • Johnson, Gary
    • Affiliation: School of Medicine, Department of Pharmacology, N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
  • Malone, Michael H
    • Affiliation: School of Medicine, Department of Pharmacology, N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
Abstract
  • Abstract Background The zebrafish Danio rerio is an important model system for drug discovery and to study cardiovascular development. Using a laser-scanning confocal microscope, we have developed a non-invasive method of measuring cardiac performance in zebrafish embryos and larvae that obtains cardiovascular parameters similar to those obtained using Doppler echocardiography in mammals. A laser scan line placed parallel to the path of blood in the dorsal aorta measures blood cell velocity, from which cardiac output and indices of vascular resistance and contractility are calculated. Results This technique, called laser-scanning velocimetry, was used to quantify the effects of pharmacological, developmental, and genetic modifiers of cardiac function. Laser-scanning velocimetry was applied to analyze the cardiovascular effects of morpholino knockdown of osmosensing scaffold for MEKK3 (OSM), which when mutated causes the human vascular disease cerebral cavernous malformations. OSM-deficient embryos had a constricted aortic arch and markedly increased peak cell velocity, a characteristic indicator of aortic stenosis. Conclusion These data validate laser-scanning velocimetry as a quantitative tool to measure cardiovascular performance for pharmacological and genetic analysis in zebrafish, which requires no specialized equipment other than a laser-scanning confocal microscope.
Date of publication
Identifier
  • doi:10.1186/1472-6750-7-40
  • 17623073
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Michael H Malone et al.; licensee BioMed Central Ltd.
License
Journal title
  • BMC Biotechnology
Journal volume
  • 7
Journal issue
  • 1
Page start
  • 40
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1472-6750
Bibliographic citation
  • BMC Biotechnology. 2007 Jul 10;7(1):40
Access
  • Open Access
Publisher
  • BioMed Central Ltd
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