MicroRNA expression distinguishes SCLC from NSCLC lung tumor cells and suggests a possible pathological relationship between SCLCs and NSCLCs Public Deposited

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Creator
  • Gazdar, Adi F
    • Other Affiliation: Simmons Comprehensive Cancer Center; Hamon Center for Therapeutic Oncology Research; Department of Pathology, UT Southwestern Medical Center, Dallas, Texas, USA
  • Minna, John D
    • Other Affiliation: Simmons Comprehensive Cancer Center; Hamon Center for Therapeutic Oncology Research; Department of Pharmacology; Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas, USA
  • Hammond, Scott
    • Affiliation: N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center, School of Medicine
  • Du, Liqin
    • Other Affiliation: Simmons Comprehensive Cancer Center, UT Southwestern Medical Center, Dallas, Texas, USA
  • Irnov
    • Other Affiliation: Division of Basic Sciences, Southwestern Graduate School of Biomedical Sciences, UT Southwestern Medical Center, Dallas, Texas, USA
  • Pertsemlidis, Alexander
    • Other Affiliation: Simmons Comprehensive Cancer Center; McDermott Center for Human Growth and Development; Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas, USA
  • Girard, Luc
    • Other Affiliation: Hamon Center for Therapeutic Oncology Research; Department of Pharmacology, UT Southwestern Medical Center, Dallas, Texas, USA
  • Schageman, Jeoffrey J
    • Other Affiliation: McDermott Center for Human Growth and Development, UT Southwestern Medical Center, Dallas, Texas, USA
Abstract
  • Abstract Background Recent studies have shown that microRNAs (miRNAs) play roles in tumorigenesis and are reliable classifiers of certain cancer types and subtypes. However, the role of miRNAs in the pathogenesis and diagnosis of small cell carcinoma (SCLC), the majority of which represent the most aggressive lung tumors, has not been investigated. Methods In order to explore miRNA involvement in the pathogenesis of small cell lung carcinoma (SCLC) and the potential role of miRNAs in SCLC diagnosis, we compared the miRNA expression profile of a set of SCLC cell lines to that of a set of non-small cell lung cancer (NSCLC) cell lines and normal immortalized human bronchial epithelial cells (HBECs) using microarray analysis. Results Our results show that miRNA profiles reliably distinguish SCLC cell lines from NSCLC and HBEC cell lines. Further analysis of the miRNA expression profile of the two subtypes of lung cancer cell lines indicates that the expression levels of the majority of the miRNAs that are differentially expressed in SCLC cells relative to NSCLC cells and HBECs show a progressive trend from HBECs to NSCLC cells to SCLC cells. Conclusions The distinctive miRNA expression signature of SCLCs relative to NSCLCs and HBECs suggests that miRNA profiles have the potential to serve as a diagnostic marker of SCLC lung tumors. The progressive trend of miRNA profile changes from HBECs to NSCLCs to SCLCs suggests a possible pathological relationship between SCLCs and NSCLCs, and suggests that the increasing dysregulation of miRNA expression may play a role in lung tumor progression. The specific role of these miRNAs in lung tumor pathogenesis and differentiation need to be investigated further in future studies.
Date of publication
Identifier
  • 20624269
  • doi:10.1186/1756-9966-29-75
Resource type
  • Article
Rights statement
  • In Copyright
Rights holder
  • Liqin Du et al.; licensee BioMed Central Ltd.
License
Journal title
  • Journal of Experimental & Clinical Cancer Research
Journal volume
  • 29
Journal issue
  • 1
Page start
  • 75
Language
  • English
Is the article or chapter peer-reviewed?
  • Yes
ISSN
  • 1756-9966
Bibliographic citation
  • Journal of Experimental & Clinical Cancer Research. 2010 Jun 17;29(1):75
Access
  • Open Access
Publisher
  • BioMed Central Ltd
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