B and T Cell Phenotypic Profiles of African HIV-Infected and HIV-Exposed Uninfected Infants: Associations with Antibody Responses to the Pentavalent Rotavirus Vaccine

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  • Weinberg, Adriana
    • Other Affiliation: Department of Pediatrics; Section of Pediatric Infectious Diseases; University of Colorado Anschutz Medical Campus
  • Lindsey, Jane
    • Other Affiliation: Center for Biostatistics in AIDS Research; Harvard School of Public Health
  • Bosch, Ronald
    • Other Affiliation: Center for Biostatistics in AIDS Research; Harvard School of Public Health
  • Persaud, Deborah
    • Other Affiliation: W. Harry Feinstone Department of Molecular Microbiology and Immunology; Johns Hopkins Bloomberg School of Public Health
  • Sato, Paul
    • Other Affiliation: Maternal Adolescent and Pediatric Research Branch; NIAID; NIH
  • Ogwu, Anthony
    • Other Affiliation: Trinity Medical Centre
  • Asmelash, Aida
    • Other Affiliation: Botswana Harvard AIDS Institute
  • Bwakura-Dangarambezi, Mutsa
    • Other Affiliation: Department of Paediatrics and Child Health; University of Zimbabwe College of Health Sciences
  • Chi, Benjamin H.
    • Affiliation: School of Medicine, Department of Obstetrics and Gynecology
  • Canniff, Jennifer
    • Other Affiliation: Department of Pediatrics; Section of Pediatric Infectious Diseases; University of Colorado Anschutz Medical Campus
  • Lockman, Shahin
    • Other Affiliation: Department of Pediatrics; Section of Pediatric Infectious Diseases; University of Colorado Anschutz Medical Campus
  • Gaseitsiwe, Simani
    • Other Affiliation: Department of Pediatrics; Section of Pediatric Infectious Diseases; University of Colorado Anschutz Medical Campus
  • Moyo, Sikhulile
    • Other Affiliation: Botswana Harvard AIDS Institute Partnership
  • Smith, Christiana Elizabeth
    • Other Affiliation: Department of Pediatrics; Section of Pediatric Infectious Diseases; University of Colorado Anschutz Medical Campus
  • Moraka, Natasha O.
    • Other Affiliation: Botswana Harvard AIDS Institute Partnership
  • Levin, Myron J.
    • Other Affiliation: Department of Pediatrics; Section of Pediatric Infectious Diseases; University of Colorado Anschutz Medical Campus
Abstract
  • We examined associations between B and T cell phenotypic profiles and antibody responses to the pentavalent rotavirus vaccine (RV5) in perinatally HIV-infected (PHIV) infants on antiretroviral therapy and in HIV-exposed uninfected (PHEU) infants enrolled in International Maternal Pediatric Adolescent AIDS Clinical Trials P1072 study ({"type":"clinical-trial","attrs":{"text":"NCT00880698","term_id":"NCT00880698"}}NCT00880698). Of 17 B and T cell subsets analyzed, PHIV and PHEU differed only in the number of CD4+ T cells and frequency of naive B cells, which were higher in PHEU than in PHIV. In contrast, the B and T cell phenotypic profiles of PHIV and PHEU markedly differed from those of geographically matched contemporary HIV-unexposed infants. The frequency of regulatory T and B cells (Treg, Breg) of PHIV and PHEU displayed two patterns of associations: FOXP3+ CD25+ Treg positively correlated with CD4+ T cell numbers; while TGFβ+ Treg and IL10+ Treg and Breg positively correlated with the frequencies of inflammatory and activated T cells. Moreover, the frequencies of activated and inflammatory T cells of PHIV and PHEU positively correlated with the frequency of immature B cells. Correlations were not affected by HIV status and persisted over time. PHIV and PHEU antibody responses to RV5 positively correlated with CD4+ T cell counts and negatively with the proportion of immature B cells, similarly to what has been previously described in chronic HIV infection. Unique to PHIV and PHEU, anti-RV5 antibodies positively correlated with CD4+/CD8+FOXP3+CD25+% and negatively with CD4+IL10+% Tregs. In conclusion, PHEU shared with PHIV abnormal B and T cell phenotypic profiles. PHIV and PHEU antibody responses to RV5 were modulated by typical HIV-associated immune response modifiers except for the association between CD4+/CD8+FOXP3+CD25+Treg and increased antibody production.
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  • Article
Rights statement
  • In Copyright
Journal title
  • Frontiers in Immunology
Journal volume
  • 8
Language
  • English
ISSN
  • 1664-3224

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