Differentially expressed plasmatic microRNAs in Brazilian patients with Coronavirus disease 2019 (COVID-19): preliminary results

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Creator
  • Nicoletti A.S.
    • Other Affiliation: University of Campinas
  • Visacri M.B.
    • Other Affiliation: University of Campinas
  • da Ronda C.R.S.C.
    • Other Affiliation: University of Campinas
  • Vasconcelos P.E.N.S.
    • Other Affiliation: University of Campinas
  • Quintanilha J.C.F.
    • Affiliation: Eshelman School of Pharmacy
  • de Souza R.N.
    • Other Affiliation: University of Campinas
  • Ventura D.S.
    • Other Affiliation: Hospital Estadual Sumaré Dr. Leandro Francheschini
  • Eguti A.
    • Other Affiliation: Hospital Estadual Sumaré Dr. Leandro Francheschini
  • Silva L.F.S.
    • Other Affiliation: Hospital Estadual Sumaré Dr. Leandro Francheschini
  • Perroud Junior M.W.
    • Other Affiliation: University of Campinas
  • Catharino R.R.
    • Other Affiliation: University of Campinas
  • Reis L.O.
    • Other Affiliation: University of Campinas
  • dos Santos L.A.
    • Other Affiliation: Hospital Municipal de Paulínia
  • Durán N.
    • Other Affiliation: University of Campinas
  • Fávaro W.J.
    • Other Affiliation: University of Campinas
  • Lancellotti M.
    • Other Affiliation: University of Campinas
  • da Costa J.L.
    • Other Affiliation: University of Campinas
  • Moriel P.
    • Other Affiliation: University of Campinas
  • Pincinato E.C.
    • Other Affiliation: University of Campinas
Abstract
  • Background: Coronavirus disease 2019 (COVID-19) is caused by a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is known that host microRNAs (miRNAs) can be modulated to favor viral infection or to protect the host. Herein, we report preliminary results of a study aiming at identifying differentially expressed plasmatic miRNAs in Brazilian patients with COVID-19. Methods and results: miRNAs were extracted from the plasma of eight patients with COVID-19 (four patients with mild COVID-19 and four patients with severe/critical COVID-19) and four healthy controls. Patients and controls were matched for sex and age. miRNA expression levels were detected using high-throughput sequencing. Differential miRNA expression and enrichment analyses were further evaluated. A total of 18 miRNAs were differentially expressed between patients with COVID-19 and controls. miR-4433b-5p, miR-6780b-3p, miR-6883-3p, miR-320b, miR-7111-3p, miR-4755-3p, miR-320c, and miR-6511a-3p were the most important miRNAs significantly involved in the PI3K/AKT, Wnt/β-catenin, and STAT3 signaling pathways. Moreover, 42 miRNAs were differentially expressed between severe/critical and mild patients with COVID-19. miR-451a, miR-101-3p, miR-185-5p, miR-30d-5p, miR-25-3p, miR-342-3p, miR-30e-5p, miR-150-5p, miR-15b-5p, and miR-29c-3p were the most important miRNAs significantly involved in the Wnt/β-catenin, NF-κβ, and STAT3 signaling pathways. Conclusions: If validated by quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in a larger number of participants, the miRNAs identified in this study might be used as possible biomarkers for the diagnosis and severity of COVID-19.
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  • Article
Rights statement
  • In Copyright
Journal title
  • Molecular Biology Reports
Journal volume
  • 49
Journal issue
  • 7
Page start
  • 6931
Page end
  • 6943
Language
  • English
Funder
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, (88887.504453/2020-00, 88887.511334/2020-00, 88887.513100/2020-00)
  • Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, (2021/04669-9)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES, (001—88881.504454/2020-01, 88887.506965/2020-00)
ISSN
  • 0301-4851
Publisher
  • Springer Science and Business Media B.V.

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