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Last modified date
  • December 16, 2019
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Abstract
  • Brown adipose tissue (BAT) is a fat tissue present in most mammals, which is specialized in non-shivering thermogenesis. Thermogenic activity in BAT is commonly detected by PET/CT imaging with 18F-FDG, a glucose analog, in both rodents and humans. Increased uptake of the 18F-FDG within regions of BAT is interpreted as an indicator of increased thermogenic activity within the tissue. Despite its prevalence, recent studies have begun to question the use of 18F-FDG PET/CT for the detection of BAT thermogenesis, casting doubt on the direct relationship between glucose uptake and the degree of thermogenic activity. To this end, this study aims to compare 18F-FDG uptake within brown fat of mice that have very different BAT thermogenic capacity: wild type mice with functional BAT and mice lacking uncoupling protein 1– a protein in which brown fat thermogenesis is believed to be contingent upon. For this study, an imaging protocol was optimized to minimize variability in BAT stimulation. The data showed that 18F-FDG uptake in mice is highly variable, even under well-controlled experimental conditions. These results clearly indicate that, in general, the magnitude of 18F-FDG uptake in brown fat cannot be used as a surrogate measure for brown adipose tissue thermogenic activity.
Methodology
  • All recorded data are listed on corresponding sheets within this excel spreadsheet. These data can be used for different statistical analysis. For our study, we did the following: Statistical analysis of the data was done by using the JMP Pro (Version 14, SAS Institute Inc., Cary, NC) software. All means are presented with their corresponding standard deviation. To compare 18F-FDG uptake, significant differences with p<0.05 between groups were determined using one-way ANOVA with Tukey-Kramer HSD for multiple comparisons. Matched-pairs t-tests were also used to evaluate the effect of NE on the thermogenic activity in WT and KO mice according to glucose uptake within BAT, rectal temperature, and surface temperature. Bartlett’s test was used to determine a difference in variance in SUVpeak with and without NE injection. The area under the curve (AUC) generated from a receiver operated characteristic (ROC) curve and F-scores were calculated to determine how well SUVpeak can sort active from inactive BAT.
Date of publication
DOI
Kind of data
  • Numeric
Resource type
  • Dataset
License
  • CC0 1.0 Universal
Language
  • English

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file details FDGUptakeData.xlsx 2020-03-17 Public Download