Investigation of the molecular mechanisms linking cell-fate specification and shape change in C. elegans gastrulation

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  • March 20, 2019
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  • Roberts, Allyson
    • Affiliation: School of Medicine, Curriculum in Genetics and Molecular Biology
Abstract
  • There is incredible diversity amongst eukaryotic tissues and overall body plans. Changes in individual cell shapes are essential for generating higher levels of complexity through morphogenetic events. Various molecular mechanisms are responsible for driving cell shape changes during development under tight spatial and temporal regulation. Apical constriction is a process by which cells shrink their apical surfaces through actomyosin contractions to orchestrate tissue-bending events including vertebrate neural tube formation and gastrulation. C. elegans is a valuable system for studying mechanisms that drive apical constriction in vivo, including how one endodermal cell fate-specifying transcription factor, END-3, drives apical constriction of two endodermal cells, thus beginning gastrulation of the embryo. Here, we screened for new gastrulation genes from among genes regulated by END-3 using RNAi and identified 7 new candidate END-3 targets that contribute to gastrulation. We predict that some of these genes may directly or indirectly regulate apical constriction and gastrulation.
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  • In Copyright
Advisor
  • Duronio, Bob
  • McKay, Dan
  • Goldstein, Bob
  • Maddox, Amy
  • Arthur, Janelle
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2018
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