Expression of interleukin-37 (IL37) and the genetic variations of IL37 in relation to chronic periodontitis

Expression of interleukin-37 (IL37) and the genetic variations of IL37 in relation to chronic periodontitis Public Deposited

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Zhang, Shaoping
- Affiliation: School of Dentistry, Department of Periodontology

Abstract

Objectives: To study the transcription of IL37 in gingival biopsies and the association between transcription of IL1B, IL6 and IL37 and genotypes at the rs3811046 and rs3811047 IL37 loci. Materials and Methods: Gingival biopsies were collected from chronic periodontitis patients and control subjects. Pyrosequencing was applied for genotyping. Real-time PCR was used to examine the transcription of genes from biopsies and cell culture models. Results: Transcription of IL6 was significantly higher in periodontitis samples. Transcription of IL37 was induced in oral epithelial and THP.1 cells by LPS. The presence of a minor allele at both loci in IL37 was significantly related to a reduced IL6 transcription in Caucasians, while the major allele was associated with a higher transcription of IL6 in African Americans. Conclusion: Gingival epithelium and monocytic cells are the major source for IL-37. The genetic variants of IL37 related to the altered transcription of IL6 are race specific.

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