Annotation of the Understudied Kinome and Preliminary Testing of Kinase Inhibitor Combinations Public Deposited

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Last Modified
  • March 20, 2019
Creator
  • Hall, Claire
    • Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering
Abstract
  • A technique utilizing multiplexed inhibitor beads and mass spectrometry (MIB/MS) detects functional protein kinases in breast cancer cell lines. Data from this technique was used to shed light on the understudied kinome, a portion of which is captured by the MIB/MS method. Regression analysis was performed to find correlations in kinase activity. The functional linkages were then used to annotate the understudied kinases. Annotations revealed new possible functions and disease relations for many understudied kinases. Kinase inhibitor combinations were suggested by principle components analysis (PCA) results performed on MIB/MS data from treated breast cancer cell lines. The combinations were preliminarily tested for signs of effectiveness. Dose curves and growth assays were performed to compare drug combinations in the SKBR3 cell line. The interpretation of in vitro experiment results was impeded because of poor accuracy and reproducibility. Possible designs for in vitro experiments producing interpretable results are presented.
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Rights statement
  • In Copyright
Advisor
  • Macdonald, Jeffrey
  • Gomez, Shawn
  • Walker, Glenn
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2017
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