Short-term Feeding Effects of High-Fat Diet on Hippocampal Neuroinflammation: Differences among C57BL/6J, C3H/HeN, and C3H/HeJ mice

Short-term Feeding Effects of High-Fat Diet on Hippocampal Neuroinflammation: Differences among C57BL/6J, C3H/HeN, and C3H/HeJ mice Public Deposited

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Millette, Drew
- Affiliation: Gillings School of Global Public Health, Department of Nutrition

Abstract

Neurodegenerative diseases represent a growing public health concern. Increasing evidence has implicated a potential role of dietary intake of saturated fats in Alzheimer’s development, possibly through Toll-like Receptor 4 (TLR-4) activation in microglia. We hypothesized that a diet high in saturated fats would increase neuroinflammation, and loss of TLR-4 would protect against this increase. C57BL/6, C3H/HeN, and C3H/HeJ mice were given either a low-fat or a high-fat diet for 8 weeks. HFD increased the expression of TNF-α and decreased expression of IL-10 in the hippocampus of C57BL/6 mice, and increased microglial TLR-2 and TLR-4 receptors. HFD did not increase neuroinflammation in C3H/HeN mice; however, IL-6, MIF and SOCS-3 transcription were decreased. No differences were discovered in C3H/HeJ mice. While microglia isolated from HFD-fed C3H/HeN mice had increased TLR-2 and TLR-4 expression, TNF-α was decreased following LPS-stimulation. In conclusion, C57BL/6 mice are an appropriate model for short-term HFD-induced hippocampal neuroinflammation.

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