Salivary inflammatory biomarkers in a stent-induced biofilm overgrowth model Public Deposited

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  • March 21, 2019
  • Stella, Michael
    • Affiliation: School of Dentistry, Department of Periodontology
  • Introduction: Salivary diagnostics is an emerging field that has promise for improving understanding, diagnosis, and treatment of periodontitis. Inflammatory biomarker levels present in saliva have the potential to discriminate between disease states and predict disease progression. Objective: To determine whether baseline salivary inflammatory biomarkers between could discriminate between different clinical levels of disease and/or predict clinical progression over a 3-week stent-induced biofilm overgrowth (SIBO) induction period. Methods: 167 subjects ranging from healthy to periodontitis were recruited, 33 or 34 subjects per group: Healthy (BGI-H), Gingivitis (BGI-G), and Mild (BGI-P1), Moderate (BGI-P2), and Severe (BGI-P3) Periodontitis. Subjects wore stents to prevent plaque removal during brushing over one maxillary and one mandibular posterior dental sextant for 21 days. Clinical periodontal parameters and unstimulated salivary samples were collected at screening, baseline, and each week during biofilm overgrowth induction and assessed for levels of selected markers. Results: Higher salivary levels of IL-1β, MMP-3, MMP-8, MMP-9, and NGAL were found in diseased groups compared to healthy at baseline. Higher IL-1ra was found in healthy subjects at baseline. MMP-1, TIMP-1, TIMP-2, and TIMP-4 levels increased during induction across all subject groups. Conclusion: Salivary inflammatory biomarkers differ at baseline by periodontal status and show changes in the immunoregulators of host response during an experimental disease progression model.
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  • Offenbacher, Steven
  • Master of Science
Graduation year
  • 2013

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