Development and Characterization of a Focally Induced Mouse Model of Glioblastoma Multiforme Originating From Terminally Differentiated Astrocytes

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  • March 20, 2019
Creator
  • Bash, Ryan
    • Affiliation: School of Medicine, Curriculum in Genetics and Molecular Biology
Abstract
  • Glioblastoma multiforme (GBM) is a devastating tumor of the central nervous system and is the most frequently occurring primary brain tumor in adults. While much progress has been made in reducing the mortality associated with other forms of cancer, morbidity from GBM remains unchanged. The lack of progress in treatment of GBM highlights the continuing need for accurate preclinical animal models as a means of understanding pathogenesis and providing a resource for testing of potential therapies. To model the development of GBM, we have developed a system using genetically engineered mouse (GEM) models in which silent mutant alleles can be converted to active oncogenes or inactive tumor supressors using the cre-lox recombination system. Through stereotactic injection of replication incompetent lentivirus expressing Cre-recombinase into the cortex of these animals, we have assessed the impact of known effectors of astrocytoma to induce and advance tumors originating from terminally differentiated astrocytes in the context of surrounding normal brain tissue.
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  • In Copyright
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  • "... in partial fulfillment of the requirements for the degree of Master of Science in the Department of Genetics and Molecular Biology, School of Medicine."
Advisor
  • Van Dyke, Terry A.
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  • Chapel Hill, NC
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  • Open access

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