The Role of Thiocyanate in Defense of Airway Surfaces against Pseudomonas aeruginosa: Dysfunction in Cystic Fibrosis Public Deposited

Downloadable Content

Download PDF
Last Modified
  • June 7, 2019
Creator
  • Yuan, Yi
    • Affiliation: School of Dentistry, Oral and Craniofacial Biomedicine PhD Program
Abstract
  • Objectives: To understand the role of thiocyanate (SCN-) in CF airway infection, we will determine potential antimicrobial mechanisms of SCN- on P. aeruginosa and the influence of SCN- on hypochlorite (HClO) -killing (a major microbicidal mechanism of inflammatory cells) on P. aeruginosa; and compare the effects of these antimicrobial reagents on mucoid and non-mucoid strains of P. aeruginosa. Materials and Methods: The conventional strain PAO1 and its mucoidy mutant (PAM) and a mucoidy clinical isolate (C3873) of P. aeruginosa were included. Target bacteria were exposed to different treatments including H2O2, LPO/H2O2 and HClO with and without SCN-. Three distinct techniques were employed to examine the antimicrobial effects of these treatments including influence on planktonic growth, recovery of colony forming units and inhibition of growth on an agar surface. Results: SCN with LPO is able to enhance the inhibitory effect of H2O2 on P. aeruginosa, due to conversion to the antimicrobial species OSCN. SCN can also rescue the P. aeruginosa from HClO-killing at optimal concentrations. The mucoidy character did not afford protection to P. aeruginosa from inhibition by H2O2, HClO or LPO/SCN/H2O2 system under either planktonic or biofilm condition. Conclusion: SCN might play a role in airway defense by preventing P. aeruginosa infection. Diminshed amounts of SCN- resulting from CFTR mutation may contribute to CF lung damage.
Date of publication
Keyword
Subject
DOI
Identifier
Resource type
Rights statement
  • In Copyright
Advisor
  • Arnold, Roland
  • Henke, David
  • Barros, Silvana
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2015
Language
Publisher
Place of publication
  • Chapel Hill, NC
Access
  • There are no restrictions to this item.
Parents:

This work has no parents.

Items